Compositions for reducing noxious sensation and/or other health benefits in infants and young children
A technology of composition, nutritional composition, applied in the direction of application, food science, function of food ingredients, etc.
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Embodiment 1
[0210] Example 1 HMO 2'FL increases the amount of KYNA (kynuric acid) produced by the microflora Fecal fermentation and kynurine Acid quantification
[0211] Fecal samples from 10 3- to 4-month-old infants (7 breast-fed (BF), 3 formula-fed (FF)) were used to inoculate YCFA medium without carbohydrates (without CHO) . The study design involved supplementing CHO-free YCFA medium with different carbohydrates at a final concentration of 0.5%, either lactose alone (F1 control) or a combination of 2'FL, 2'FL and LnNT, or Combinations of 2'FL, diFL, 3'SL, 6'SL, LNT and LNnT. Four replicate fermentations (F1 to F4) were performed on each individual and supernatants were collected after 24 and 48 hours. YCFA is a complex medium containing tryptone, yeast extract and various minerals, vitamins and short chain fatty acids; however, typical CHO components (glucose, maltose and cellobiose) are not included in this example.
[0212] For metabolite analysis, samples were extracted and...
Embodiment 2
[0216] 6′SL+LNT and KYNA have additive effects on GPR35 activation
[0217] method
[0218] Cell lines stably expressing GPR35 were expanded from frozen stocks according to standard procedures. Cells were seeded into white-walled 384-well microplates in a total volume of 20 μL and incubated at 37° C. for the appropriate time prior to testing. All tests are performed in duplicate. On each test day, 5x compound working intermediates were prepared in phosphate buffered saline (PBS) for each test concentration. 5 μL of 5x sample was added to cells and incubated for 90 minutes at 37°C or room temperature. Assay signals were generated by a single addition of 12.5 μL or 15 μL (50% v / v) PathHunter Detection Reagent Mix (DiscoverX) followed by one hour incubation at room temperature. Microplates were read after signal generation with a PerkinElmer EnvisionTM instrument for chemiluminescent signal detection. Compound activity was analyzed using the CBIS Data Analysis Suite (Che...
Embodiment 3
[0223] Table 1 below gives an example of the composition of a nutritional composition (eg, infant formula) according to the present invention. The composition is given by way of illustration only.
[0224] Table 1 : Composition of the infant formula of Example 1
[0225]
[0226]
PUM
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