Alternaria alternata, herbicide and application thereof
A technology for isolating Alternaria alternata and herbicides, applied in the field of microorganisms, achieving good development prospects, simple preparation methods, and high selectivity
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Embodiment 1
[0069] Example 1 Isolation and identification of Alternaria alternata
[0070] 1. Collection of diseased plants of Artemisia annua
[0071] The susceptible plants of Artemisia somnifera were collected in Jimo District, Qingdao City, Shandong Province in June 2020. The aerial parts of the susceptible plants were cut off and dried indoors for storage.
[0072] 2. Cultivation of Artemisia annua
[0073] Select the seeds of Artemisia spp. after the low-temperature stratification treatment, disinfect 2m with 0.1% mercuric chloride solution, wash with sterile water several times, sow them in small flowerpots equipped with loose soil, and place them in a greenhouse after covering with soil (15- 25°C), grow to the seedling stage and then transplant, one plant in each pot, and continue to cultivate until bolting.
[0074] 3. Isolation and identification of pathogenic bacteria of Artemisia somnifera
[0075] (1) Isolation and purification of strains
[0076] Cut out about 0.2cm × 0....
Embodiment 2
[0090] Example 2 Mycelial growth characteristics of Alternaria alternata JM strain
[0091] 1. The effect of culture medium on the growth of Alternaria alternata JM strain mycelium
[0092] (1) Experimental method: activate the bacterial strain according to the conditions of separation and purification of the aforementioned bacterial strain, when the diameter of the colony reaches 80 mm, take a bacterial cake with a diameter of 6 mm, transfer it to a different medium, and cultivate it in the dark at a constant temperature of 28 ° C for 7 d. Colony diameter was measured by the criss-cross method. A total of 7 media, PDA, PSA, CA, OMA, SDA, CMA and WA, were investigated, and each treatment was repeated 3 times.
[0093] (2) Experimental results and analysis: from Figure 8 The results showed that the JM strain could grow on all 7 kinds of media, and it grew the fastest on PSA and PDA media, and the hyphae grew densely. in other media, but there was no significant difference b...
Embodiment 3
[0109] Embodiment 3 Alternaria alternata JM strain solid culture sporulation characteristics
[0110] 1. Preparation of solid medium
[0111] A 250mL conical flask was used as the fermentation container, the bottling amount was 20.00g / bottle, the water mixing amount was 75%, pre-soaked for 24 hours, sterilized at 121° C. for 20 m and used for later use.
[0112] 2. Fermentation culture and spore content determination method
[0113] Unless otherwise mentioned, all were carried out in this way. Two 10 mm diameter bacterial blocks of Alternaria alternans JM strain were taken, inoculated into the medium, and incubated at 28°C in the dark for 7 days, and each treatment was repeated 3 times. Take all the medium and add it to 50 mL of sterile water with glass beads, let stand for 30 minutes, shake at 200 rpm for 30 minutes, filter with four layers of gauze, drop the spore suspension onto a hemocytometer, observe under a microscope, and record 5 medium grids The total number of sp...
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