Method for improving transplanting survival rate and fragrance of pandan leaf tissue culture seedlings
A technology for transplanting survival rate and pandan leaf group, which is applied in the field of improving the transplanting survival rate and fragrance of pandan leaf tissue culture seedlings, and can solve the problem of many steps, lack of restrictions on the development of pandan leaf industry, and the inability to meet high-throughput planting requirements. Seedling breeding requirements and other issues
Active Publication Date: 2022-08-05
SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology
This method can grow the mother plant for at least two years, and the seedlings that need to be tilled are limited, which cannot meet the industry's requirements for high-throughput seedling breeding, and the lack of high-throughput seedling breeding technology has restricted the further development of the blue leaf industry. develop
In addition, after the large-scale breeding of pandan leaf tissue culture seedlings, there will also be differences in growth when transplanting, resulting in low survival rate of transplanting, many steps from hardening seedlings in the bottle to hardening seedlings outside the bottle and long time, and insufficient fragrance And other issues
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Embodiment 1
[0030] Select the tissue culture seedlings with leaves with roots of 6-10cm, complete roots and 2-6 developed leaves, soaked in 50mg / ml ZnSO 4In the liquid culture for 1 day, during the period, use float to ensure that only the roots of the seedlings are soaked, and spray water mist with a watering can to ensure that the leaves of the seedlings are moist. The light intensity during treatment is 3000lx, and the light time is 10h. Seedling temperature of 28°C; after liquid culture, the tissue culture seedlings need to be transplanted into the cultivation medium containing 0.5-0.8cm sand and gravel, and the humidity of the substrate should be kept at 80%-85% to prevent the substrate from getting too wet. Water, when the substrate is not dry, use an aqueous solution containing 10% ammonium bicarbonate + 5% calcium superphosphate + 10% potassium chloride, pour it to 70% ~ 80% humidity as the standard for seedling cultivation. The light intensity of the seedlings is 3000lx, and the ...
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The invention provides a method for improving transplanting survival rate and fragrance of pandan leaf tissue culture seedlings. The method comprises the following steps: S1) soaking roots of the pandan leaf tissue culture seedlings with roots in a zinc sulfate solution for treatment; s2) transplanting the treated pandan leaf tissue culture seedlings into a culture medium, and keeping the humidity of the culture medium at 70-85% for seedling hardening culture; and S3) performing field planting on the pandan leaf tissue culture seedlings subjected to seedling hardening culture. Compared with the prior art, the method provided by the invention has the advantages that the transplanting survival rate of the pandan leaf tissue culture seedlings can be increased, the in-bottle and out-bottle seedling culture time of the tissue culture seedlings can be shortened, and the content of main aroma components, especially the content of 2-acetyl-1-pyrroline, of the pandan leaf tissue culture seedlings can be increased, so that the production efficiency and the seedling quality of the pandan leaves are effectively improved.
Description
technical field [0001] The invention belongs to the technical field of plant breeding and cultivation, and in particular relates to a method for improving the transplanting survival rate and fragrance of pandan leaf tissue culture seedlings. Background technique [0002] Pandan leaf, scientific name Pandanus amaryllifolius, English translation trade name (Pandan leaf), domestic translation is Banlanxiang, also known as pandan leaf, pandan leaf, Banlan leaf, for the Pandanaceae (Pandanaceae) Pandanus is a perennial evergreen horticultural crop. There are about 800 species of plants in 3 genera of Pandanaceae. The native ecological environment of this genus is very different, and its economic uses are different. It can be used in medical and cooking fields. There are many species in the genus that can treat toothache and rheumatism. Development and utilization prospects are broad. Among them, pandan leaves is a plant with a unique "zongxiang" fragrance in this genus. Moreov...
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Login to View More IPC IPC(8): A01H4/00A01G22/00
CPCA01H4/00A01G22/00Y02P60/14
Inventor 吉训志秦晓威鱼欢张昂贺书珍宗迎初众唐冰郝朝运闫林
Owner SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI