Unlock instant, AI-driven research and patent intelligence for your innovation.

Coronavirus subunit vaccine for presenting coronavirus receptor binding region based on virus-like particles

A virus-like, chikungunya virus technology, applied in the field of biomedicine, can solve the problems of unclear safety and weak immunogenicity

Pending Publication Date: 2022-08-05
TSINGHUA UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immunogenicity of RBD monomer or dimer is relatively weak, and there is currently no RNA vaccine approved for marketing, and its long-term safety is still unclear. Therefore, RBD with higher safety and high immunogenicity has been developed Subunit vaccines are necessary

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Coronavirus subunit vaccine for presenting coronavirus receptor binding region based on virus-like particles
  • Coronavirus subunit vaccine for presenting coronavirus receptor binding region based on virus-like particles
  • Coronavirus subunit vaccine for presenting coronavirus receptor binding region based on virus-like particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1. Preparation of virus-like particles

[0087] 1. Construction of recombinant plasmids

[0088] The double-stranded DNA molecule shown in SEQ ID NO: 6 was substituted for the small fragment between KpnI and XhoI of the pcDNA3.1(+) vector to obtain the recombinant plasmid pcDNA3.1-CHIKV-VLP. The double-stranded DNA molecule shown in SEQ ID NO:6 encodes the CHIKV polyprotein shown in SEQ ID NO:2 (see the schematic diagram of the structural domain). figure 1 ). In the CHIKV polyprotein shown in SEQ ID NO: 2, the 1-261 amino acid residues constitute the capsid protein, the 262-321 amino acid residues constitute the E3 protein, and the 322-325 amino acid residues constitute the Furin cleavage site , amino acid residues 326-747 constitute E2 protein, amino acid residues 748-809 constitute 6K protein, and amino acid residues 810-1248 constitute E1 protein. CHIKV polyprotein is cleaved by host proteases to produce capsid protein, E1 protein, E2 protein, E3 protein, ...

Embodiment 2

[0111] Example 2. Evaluation of immune effect

[0112] Imject Alum: Thermo Scientific Corp, Cat. No. 77161.

[0113] The CHIKV-VLP solution or the CHIKV-RBD-Flag1-VLP solution prepared in Example 1 was used to adjust the protein concentration with PBS buffer at pH 7.4.

[0114] 1. Group immunization

[0115] Balb / c female mice aged 4-6 weeks were randomly divided into three groups with 3 mice in each group. Immunization process: the first immunization was performed on the first day of the experiment, and the booster immunization was performed on the 15th day of the experiment. Immunization methods are: right hind limb intramuscular injection. A single mouse was immunized with 100 μl of the immunized material at a time. CHIKV-VLP group: each 100 μl of the immune substance was emulsified by 50 μl of CHIKV-VLP solution (with a protein content of 2 μg) and 50 μl of aluminum glue adjuvant. CHIKV-RBD-Flag1-VLP group: each 100 μl of the immune substance was obtained by emulsific...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a coronavirus subunit vaccine for presenting a coronavirus receptor binding region based on virus-like particles. The present invention provides virus-like particles presenting specific proteins. The specific protein is an RBD (Radial Basis Decision) segment of S protein of SARS-CoV-2. The virus-like particles presenting the specific protein are obtained by performing the following modification on chikungunya virus-like particles: inserting the specific protein into E2 protein of chikungunya virus. The insertion position specifically can be between the 204 amino acid residue and the 205 amino acid residue of the E2 protein of the chikungunya virus. The virus-like particles can be used for preparing products. The application of the product is as follows: (e1) the product is used as a novel coronavirus vaccine; (e2) as a medicine for preventing and / or treating new coronal pneumonia. The method disclosed by the invention has important application and popularization values on the SARS-CoV-2.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a coronavirus subunit vaccine that presents a coronavirus receptor binding region based on virus-like particles. Background technique [0002] Coronavirus is an enveloped positive-sense RNA virus belonging to the family Coronaviridae. Some coronaviruses seriously threaten human health, such as SARS-CoV that causes the outbreak of severe respiratory syndrome (SARS) and MERS-CoV that causes the outbreak of Middle East respiratory syndrome (MERS), and SARS-CoV that causes the new coronavirus pneumonia (COVID-19) CoV-2. In addition, there are some coronaviruses that cause milder diseases in humans, such as HCoV-NL63, HCoV-229E, HCoV-OC43, and HCoV-HKU1. Some coronaviruses can also infect animals and have an impact on pet health and livestock production, such as feline peritonitis virus (FIPV), which causes peritonitis and ascites in cats, with high fatality rates, and porcine epi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/85A61K39/215A61P31/14A61P11/00
CPCC07K14/005C12N15/85A61K39/12A61P31/14A61P11/00C12N2770/20022C12N2770/20034C12N2770/36122C12N2770/36123C12N2770/36152C07K2319/00C07K2319/43C12N2800/107A61K2039/5258A61K2039/53Y02A50/30
Inventor 向烨汪林荣苗
Owner TSINGHUA UNIV