A recombinant bacterium for producing L-tyrosine and its construction method and application

By introducing specific enzyme genes into the host bacteria, building recombinant bacteria, using benzaldehyde and glycine as substrates, the efficient and green and environmentally friendly production of L-tyrosine is achieved, and the problem of using toxic substrates in the existing technology is solved, and there is good industrial application prospects.

CN116042500BActive Publication Date: 2025-03-25HANGZHOU VIABLIFE BIOTECH CO LTD
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Patent Information

Application Number
CN202211665015.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-23
Publication Date
2025-03-25
Estimated Expiration
2042-12-23

AI Technical Summary

Technical Problem

In the existing L-tyrosine preparation methods, phenol or formaldehyde, which is highly toxic, is used as substrates, resulting in safety management challenges during the production process and possible residual harmful substances in the finished product, limiting its application scope.

Method used

Recombinant bacteria were constructed by introducing genes encoding threonine aldolase, threonine deaminase, leucine dehydrogenase, phenylalanine hydroxylase, pterin 4a-methanolamine dehydrase and dihydropterin reductase into the host bacteria, and L-tyrosine was synthesized through a series of enzymatic reactions using benzaldehyde and glycine as substrates.

Benefits of technology

It realizes efficient and green environmentally friendly production of L-tyrosine, avoids the use of toxic substrates, reduces production costs, and has good industrial application prospects.

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Abstract

This invention discloses a recombinant bacterium for producing L-tyrosine, its construction method, and its applications. The genetically engineered bacterium of this invention incorporates genes for threonine aldolase, threonine deaminase, leucine dehydrogenase, phenylalanine hydroxylase, pterin 4α-methanolamine dehydratase, and dihydropterin reductase, enabling its use in the biosynthesis of L-tyrosine. This invention also discloses a method for constructing and applying a recombinant *Escherichia coli* engineered bacterium. The production of L-tyrosine using the recombinant bacterium of this invention is highly efficient, environmentally friendly, and low-cost, showing promising prospects for industrial application.
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