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Use of SR-15 protein molecules

A protein molecule, SR-15 technology, applied in the field of protein molecules

Inactive Publication Date: 2004-08-18
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic and foreign experts in this field have recognized that virus-binding receptors will indeed introduce specific signals to cells, but the genetic response caused by this signal—especially the early genetic response has no effect on the virus in the cell or adjacent to the cell. Various effects of the infection process in the same type of cells have not been specifically explored

Method used

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  • Use of SR-15 protein molecules
  • Use of SR-15 protein molecules
  • Use of SR-15 protein molecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Such as figure 1 As shown, mRNA differential display analysis was performed on KMB-17 cells stimulated by HSVI for 1 hour, and specific EST fragment probes were prepared. In the hybridization screening experiment of the KMB-17 cell cDNA library established under the same conditions, it was found that The positive clone HSP-975 was named SR-15.

Embodiment 2

[0039] Such as figure 2 As shown, the SR-15 protein cloned into the pGEX-5T expression vector was well expressed in fusion, and the fusion expression product was subjected to affinity chromatography. figure 2 Middle: 1. Expression of SR-15 in pGEX-5T; 2. Control; 3. Protein Marker.

Embodiment 3

[0041] Experimental study on the structural and functional characteristics of SR-15 protein. The structure of SR-15 protein is as follows: there is an RS repeat region at the N-terminus, a PPLP domain in the middle, and more arginine and glycine as basic characteristics. The data show that the RS repeat region is a characteristic of the SR protein family, which is an important component in the process of intracellular pre-mRNA splicing, but the RS repeat domain in its structure is located at the C-terminus of the molecule and has a characteristic The RNA recognition region (RRM); the PPLP region is a characteristic structure that binds to Src-like proteins (see figure 1 ). 35 S and 32 The results of P immunoprecipitation showed that SR-15 protein was expressed and phosphorylated after HSVI stimulated cells, suggesting that SR-15 protein, as an intermediary factor, mediated the relationship between virus infection and cell cycle. See the result image 3 .

[0042] exist ...

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Abstract

The present invention relates to a protein molecular with the function of inhibiting replication of herpes simplex virus I type and its preparation method, belonging to the field of molecular biology technology. The total length of said protein molecule gene is 904bp, it possesses intact 5'-non-coding region and 3'-non-coding region, its coding region can code one protein formed from 121 amino acids, and its molecular weight is 14.9 KD. Said protein can be structurally expressed as follows: N end possesses one RS iteron, its middle position has has one PPLP structural domain, and it is basically characterized by arginine containnig 16.5% and serine containing 12.4%.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a protein molecule specifically induced by herpes simplex virus after binding to a cell receptor. Background technique [0002] Herpes simplex virus (HSVI) is a highly infectious double-stranded DNA virus with a complex structure. During its infection of the human body, it has special pathological characteristics-latent infection of ganglion and epithelial cells and repeated manifestations onset, and certain potential carcinogenic properties. It has a variety of antigenic components and exhibits a variety of interactions with cells during intracellular proliferation. This area is a hotspot of virology research. However, the genetic responses caused by the interaction between HSVI and receptors and the significance of such responses in the process of virus infection are still unknown. [0003] Some experiments have shown that HSV will show diff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P31/22C07K14/00C07K14/435C07K14/47C12N7/01C12N15/31
Inventor 李琦涵董承红王丽春刘龙丁赵红玲姜莉孙明王炯董少忠王晶晶赵树栋胡方易红昆
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI