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Determination for specificity of Candida albicans DNA sequence (2) and use thereof

A technology of Candida albicans and DNA sequence, which is applied in the field of biomedicine and achieves the effect of low cost and easy operation

Inactive Publication Date: 2004-09-29
KUNMING HUANJI BIOLOGICAL CHIP IND
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  • Summary
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  • Claims
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Problems solved by technology

[0002] Candida albicans is a pathogen that frequently infects the vagina of women. Accurate diagnosis is the basis for timely and reliable treatment. At present, it can only be diagnosed according to clinical indications

Method used

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  • Determination for specificity of Candida albicans DNA sequence (2) and use thereof
  • Determination for specificity of Candida albicans DNA sequence (2) and use thereof
  • Determination for specificity of Candida albicans DNA sequence (2) and use thereof

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Embodiment Construction

[0020] Firstly, the nucleotide sequence of Candida albicans was searched in the gene database with Candida albicans as the keyword. A total of several closely related nucleotide sequences were obtained, and these nucleotide sequences were compared with the nucleotide sequences (nr) in the gene database using the Blast online software on the NCBI network to exclude non-specific nucleotide sequences, A 1 Kb specific nucleotide sequence was obtained. More than 20 pairs of PCR primers were designed based on this sequence, DNA was extracted from clinical specimens, PCR amplification was performed, and a pair of sequences and primers suitable for PCR amplification were screened out. The products obtained by PCR amplification were consistent with the expected size (see Fig. 1. The serial number in the picture is: 1. Standard molecular weight DNA, the molecular weight from top to bottom is: 622, 527, 404, 309, 242 / 238, 217, 201, 190, 180, 160+160, 147+147, 123 ; 2.4.5 clinical negati...

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Abstract

The present invention utilizes the search and analysis of gene data base to define the specificity of a segmentum of Candida albicans DNA sequence (2) and PCR amplification primer. Said sequence length is 184 bp, and said sequence can be obtained by means of PCR amplification and clone of female genital tract candida albicans infection specimen. Said invention can be used as probe for gene chip diagnosis and PCR amplification detection sequence for accurately diagnosing candida albicans infection.

Description

Technical field: [0001] The invention relates to the determination and application of a specific DNA sequence (2) of Candida albicans (Candida albicans), which belongs to the technical field of biomedicine. Background technique: [0002] Candida albicans is a pathogen that frequently infects women's vagina. Accurate diagnosis is the basis for timely and reliable treatment. At present, it can only be diagnosed according to clinical indications. PCR and gene chip diagnosis is a fast and accurate detection method. Diagnostic methods of this type rely on the availability of specific nucleic acid sequences. Invention content: [0003] The invention is to determine a section of candida albicans specific nucleotide sequence and its PCR primer through bioinformatics and molecular biology techniques. The sequence and corresponding PCR primers can be used to establish gene chip and PCR methods to diagnose Candida albicans. [0004] The technical scheme of the present invention is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H21/04C12Q1/02C12Q1/68
Inventor 谭德勇朱宝生
Owner KUNMING HUANJI BIOLOGICAL CHIP IND
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