Method for detecting triglyceride double bond structural isomers

By studying the mass spectrometry fragmentation characteristics and abundance ratios of triglyceride double bond structural isomers, a comprehensive characterization strategy was constructed, which solved the problem of low efficiency in detecting triglyceride double bond structural isomers in existing technologies, and achieved efficient and accurate double bond structure characterization.

CN119534609BActive Publication Date: 2025-11-07NATIONAL INSTITUTE OF METROLOGY CHINA
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Patent Information

Application Number
CN202411706640.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-26
Publication Date
2025-11-07
Estimated Expiration
2044-11-26

AI Technical Summary

Technical Problem

Existing technologies are insufficient for efficiently and accurately detecting triglyceride double bond isomers, which hinders in-depth research into lipid structures.

Method used

By studying the positional isomers of double bonds, the characteristic fragments of cis-trans isomers by mass spectrometry, fragment abundance, and abundance ratio, and combining this with high-resolution mass spectrometry detection of triglyceride samples, a comprehensive TAG double bond isomer characterization strategy was constructed to determine the position and cis-trans structure of the double bonds.

Benefits of technology

It improves the efficiency and accuracy of triglyceride double bond structure characterization, providing support for in-depth exploration of lipid structure, and has high detection accuracy and sensitivity.

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Abstract

The application discloses a method for detecting triglyceride double bond structural isomers. The method comprises the following steps: based on the molecular ion peak [M+N]+, the fragment ion [M+N-R]+ and the fragment ion [M+N-2R]+ in the primary mass spectrum result and the secondary mass spectrum result of a triglyceride sample, comparing with a lipid compound database, so as to obtain the structure type of each branched chain of the triglyceride sample and determine the mass-to-charge ratio range of the fragment ion [M+N-R]+; based on the abundance of the acyl chain fragment ion between the molecular ion peak [M+N]+ and the fragment ion [M+N-R]+, the ratio of the abundance and the difference from the adjacent mass-to-charge ratio of the characteristic acyl chain fragment ion, so as to determine the position and cis-trans structure of each branched chain double bond. The method discloses the fine fragmentation rule of TAG double bond isomers, and constructs a comprehensive TAG double bond isomer characterization strategy.
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