Reservoir floc degradation method based on microbial technology

Abstract

The present invention provides a method for degrading reservoir flocs based on microbial technology. The method for degrading reservoir flocs based on microbial technology comprises: a. conducting a comprehensive water quality analysis of the reservoir water body, including determination of organic matter concentration, floc quantity, pH value, dissolved oxygen content, and temperature parameters, to determine the cause of reservoir flocs and their distribution characteristics, and using high-throughput sequencing technology to analyze the existing microbial community structure in the water body, identify key microbial species and their functions, so as to optimize the formulation of subsequent microbial agents. The method for degrading reservoir flocs based on microbial technology effectively solves the problem of microbial activity being limited by environmental conditions, resulting in unstable degradation efficiency, by precisely controlling the dosage of microbial agents and the strain ratio. Specifically, through comprehensive water quality analysis and evaluation of microbial community structure, the formulation of microbial agents can be optimized to better adapt to the actual environment of the reservoir.

Description

Technical Field

[0001] The invention relates to the technical field of reservoir floc degradation, in particular to a reservoir floc degradation method based on microbial technology. Background Art

[0002] Methods for degrading reservoir flocs based on microbial technology mainly include aerobic microbial degradation, anaerobic microbial degradation, the application of composite microbial agents, and biofilm technology. These methods improve water quality by selecting specific biosafety microbial strains, such as aerobic or anaerobic bacteria such as Pseudomonas and Bacillus, to decompose organic matter and flocs in water bodies under suitable environmental conditions. The use of aerobic composite agents significantly reduces the concentration of organic matter and improves water transparency; in urban drinking water source protection areas, the use of anaerobic microbial degradation reduces the organic matter content in the bottom sediment and extends the service life of the water source protection area. In addition, biofilm technology continuously degrades organic pollutants in runoff through microbial communities on fixed carriers, promoting the recovery of shore ecosystems.

[0003] However, the activity of microorganisms is restricted by environmental conditions and fluctuates, which affects the degradation efficiency of microorganisms and leads to unstable treatment effects. Although biosafe microbial agents are selected, long-term and large-scale addition may change the structure of microbial communities in the water body, thereby affecting the ecological balance of the reservoir. Summary of the Invention

[0004] In response to the shortcomings of the existing technology, the present invention provides a reservoir floc degradation method based on microbial technology, which solves the problem that the activity of microorganisms is restricted by environmental conditions and fluctuates, affecting the degradation efficiency of microorganisms and resulting in unstable treatment effects; and the problem that long-term and large-scale addition may change the structure of microbial communities in the water body and thus affect the ecological balance of the reservoir.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: A method for degrading reservoir flocs based on microbial technology comprises the following steps:

[0006] a. Conduct a comprehensive water quality analysis of the reservoir water, including measurements of organic matter concentration, floc count, pH, dissolved oxygen content, and temperature, to determine the cause and distribution of flocs in the reservoir. High-throughput sequencing technology will be used to analyze the existing microbial community structure in the water, identify key microbial species and their functions, and optimize the formulation of subsequent microbial agents.

[0007] b. Cultivate aerobic bacteria, anaerobic bacteria and auxiliary microbial strains under sterile conditions, and amplify each strain through fermentation to ensure its activity and degradation ability. Mix the ingredients according to the following mass percentages:

[0008] Aerobic strains, accounting for 40% of the total inoculum, including 20% ​​of Pseudomonas and 20% of Bacillus;

[0009] Anaerobic bacteria, accounting for 30% of the total bacterial agent, including 15% of methanogens and 15% of sulfate-reducing bacteria;

[0010] Auxiliary microorganisms, accounting for 15% of the total amount of the inoculant, including 10% of fungi and 5% of enzyme-producing bacteria;

[0011] Nutritional additives, accounting for 10% of the total inoculum, include 4% amino acid mixture, 3% potassium dihydrogen phosphate, and 3% iron, zinc, and magnesium in total;

[0012] Protective agent, accounting for 5% of the total amount of the inoculant, including 3% glycerol and 2% sucrose;

[0013] Carrier material, accounting for 10% of the total amount of the microbial agent, including 5% bentonite and 5% activated carbon;

[0014] The above ingredients are fully mixed under sterile conditions so that the microorganisms and nutrients are evenly distributed on the carrier material, and then freeze-dried to extend the shelf life of the microbial agent and facilitate transportation and storage;

[0015] c. Determine the dosage of the microbial agent based on the water organic matter concentration, floc count, and water volume obtained in the pretreatment and assessment steps, and evenly distribute the prepared microbial agent in the reservoir water to ensure uniform distribution and effective contact of the microorganisms in the water;

[0016] d. Install temperature sensors, pH sensors and dissolved oxygen sensors in the reservoir to monitor water environment parameters in real time through an automated control system;

[0017] e. Regularly collect water samples and use high-throughput sequencing technology and water quality analysis methods to monitor the microbial community structure, residual flocculent matter, and organic matter degradation rate. Based on the monitoring results, adjust the dosage of microbial agents and the strain ratio in a timely manner to avoid significant changes in the microbial community structure and maintain the ecological balance of the reservoir;

[0018] f. During the floc degradation process, intermediate products or by-products are generated, which are monitored through water sampling and analysis. Physical filtration, chemical neutralization or further biodegradation methods are used to treat the by-products to prevent secondary pollution;

[0019] g. After the degradation process is completed, conduct a comprehensive water quality assessment to confirm the floc degradation effect and the degree of water quality improvement. Prepare a detailed project evaluation report, recording the changes in various parameters during the degradation process, degradation efficiency and ecological impact, as a basis for subsequent optimization and reference;

[0020] h. Through automated control systems and regular monitoring, abnormal environmental parameters or sudden problems during the degradation process can be discovered in a timely manner. According to the preset emergency plan, rapid adjustments to the dosage, suspension of addition or other remedial measures can be taken to ensure the safety and stability of the degradation process.

[0021] Preferably, the auxiliary microorganisms include fungi and enzyme-producing bacteria, which are used to enhance the degradation efficiency of flocs by decomposing complex organic polymers and secreting a variety of enzymes. The protective agent is selected from glycerol and sucrose, which is used to improve the survival rate and adaptability of microbial agents in water bodies.

[0022] Preferably, the dosage of the microbial agent is adjusted according to the total volume of the reservoir water body and the number of flocs. The dosage of the microbial agent is 1 kg to 5 kg per cubic meter of water body, and is added in stages and batches through an automated dosing device to ensure uniform distribution of microorganisms and not cause ecological imbalance in the water body.

[0023] Preferably, the microbial agent is stored in sealed packaging to prevent the external environment from affecting the activity of the agent, and the sealed packaging material is food-grade moisture-proof and oxidation-resistant material.

[0024] Preferably, during the addition of the microbial agent, multiple microbial distribution monitoring points are set in the reservoir, and data are transmitted to the control center in real time through a wireless sensor network, thereby achieving accurate addition of microorganisms and position optimization.

[0025] Preferably, the enzyme-producing bacteria in the microbial agent include β-glucosidase, lipase and protease, wherein β-glucosidase is used to decompose cellulose substances, lipase decomposes fat pollutants, and protease is used to degrade protein organic matter in water bodies.

[0026] Preferably, the water temperature is between 20°C and 30°C, the pH value is between 6.5 and 8.5, and the dissolved oxygen concentration is above 5 mg / L to maintain the optimal activity of microorganisms.

[0027] Preferably, the water quality analysis further analyzes heavy metal pollutants in the reservoir water, detects lead, mercury, and cadmium heavy metals in the water by high performance liquid chromatography or atomic absorption spectrometry, and adjusts the ratio of microbial agents according to the test results to increase the degradation or adsorption capacity of these heavy metals.

[0028] The present invention provides a method for degrading reservoir flocs based on microbial technology. It has the following beneficial effects:

[0029] This microbial-based reservoir floc degradation method effectively addresses the problem of environmentally limited microbial activity, leading to unstable degradation efficiency, by precisely controlling the dosage and strain ratio of the microbial agent. Specifically, through comprehensive water quality analysis and assessment of microbial community structure, the formulation of the microbial agent can be optimized to better suit the actual reservoir environment. This method precisely controls the distribution of microorganisms, avoiding the potential changes in the microbial community structure caused by long-term, large-scale addition of agents, thereby effectively preventing imbalances in the reservoir ecosystem.

[0030] The use of advanced microbial cultivation and freeze-drying technologies, along with automated control systems, ensures the effectiveness and stability of the microbial inoculant in water. During the dosing process, real-time monitoring of water quality changes in the reservoir, particularly key environmental parameters such as dissolved oxygen, pH, and temperature, helps maintain optimal microbial activity. Furthermore, the sealed packaging of the microbial inoculant prevents environmental influences on its activity, ensuring long-term storage and stability during transportation, thereby enhancing the effectiveness and reliability of the degradation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] Example 1:

[0034] like Figure 1 As shown, the embodiment of the present invention provides a reservoir floc degradation method based on microbial technology, including: a. conducting a comprehensive water quality analysis of the reservoir water body, including measuring organic matter concentration, floc quantity, pH value, dissolved oxygen content, and temperature parameters to determine the cause of the reservoir floc and its distribution characteristics; using high-throughput sequencing technology to analyze the existing microbial community structure in the water body, identify key microbial species and their functions, so as to optimize the formulation of subsequent microbial agents;

[0035] b. Cultivate aerobic bacteria, anaerobic bacteria and auxiliary microbial strains under sterile conditions, and amplify each strain through fermentation to ensure its activity and degradation ability. Mix the ingredients according to the following mass percentages:

[0036] Aerobic strains, accounting for 40% of the total inoculum, including 20% ​​of Pseudomonas and 20% of Bacillus;

[0037] Anaerobic bacteria, accounting for 30% of the total bacterial agent, including 15% of methanogens and 15% of sulfate-reducing bacteria;

[0038] Auxiliary microorganisms, accounting for 15% of the total amount of the microbial agent, including 10% of fungi and 5% of enzyme-producing bacteria, are used to enhance the degradation efficiency of flocculent matter by decomposing complex organic polymers and secreting a variety of enzymes. The protective agent is selected from glycerol and sucrose and is used to improve the survival rate and adaptability of the microbial agent in the water body;

[0039] Nutritional additives, accounting for 10% of the total inoculum, include 4% amino acid mixture, 3% potassium dihydrogen phosphate, and 3% iron, zinc, and magnesium in total;

[0040] Protective agent, accounting for 5% of the total amount of the inoculant, including 3% glycerol and 2% sucrose;

[0041] Carrier material, accounting for 10% of the total amount of the microbial agent, including 5% bentonite and 5% activated carbon;

[0042] The above ingredients are fully mixed under sterile conditions so that the microorganisms and nutrients are evenly distributed on the carrier material, and then freeze-dried to extend the shelf life of the microbial agent and facilitate transportation and storage;

[0043] c. Determine the dosage of the microbial agent based on the concentration of organic matter in the water, the number of flocs, and the water volume obtained in the pretreatment and assessment steps, and evenly distribute the prepared microbial agent in the reservoir water to ensure uniform distribution and effective contact of the microorganisms in the water. The dosage of the microbial agent is adjusted according to the total volume of the reservoir water and the number of flocs. The dosage of the microbial agent is 1 kg to 5 kg per cubic meter of water, and is added in stages and batches through an automated dosing device to ensure uniform distribution of microorganisms and not cause ecological imbalance in the water body. The microbial agent is stored in sealed packaging to prevent the external environment from affecting the agent. activity, the material of the sealed packaging is a food-grade moisture-proof and anti-oxidation material, and during the addition of the microbial agent, multiple microbial distribution monitoring points are set in the reservoir, and data is transmitted to the control center in real time through a wireless sensor network, thereby achieving precise addition of microorganisms and position optimization. The enzyme-producing bacteria in the microbial agent include β-glucosidase, lipase and protease, of which β-glucosidase is used to decompose cellulose substances, lipase decomposes fat pollutants, and protease is used to degrade protein organic matter in the water body. During the addition process, a layered addition strategy is adopted in combination with different areas and different depths of the reservoir water body, such as water surface and bottom water. The dosage can be increased appropriately in shallow water areas to ensure efficient degradation of flocs in shallow water areas; in deep water areas, the optimal dosage is calculated through diffusion and flow models to ensure uniform distribution of microorganisms and degradation efficiency;

[0044] d. Install temperature sensors, pH sensors, and dissolved oxygen sensors in the reservoir to monitor water environmental parameters in real time. Through an automated control system, the water temperature is maintained between 20°C and 30°C, the pH value is between 6.5 and 8.5, and the dissolved oxygen concentration is above 5 mg / L to maintain optimal microbial activity;

[0045] e. Regularly collect water samples and utilize high-throughput sequencing technology and water quality analysis methods to monitor microbial community structure, residual flocculent matter, and organic matter degradation rate. Based on the monitoring results, timely adjust the dosage and strain ratio of microbial agents to avoid significant changes in the microbial community structure and maintain the ecological balance of the reservoir. The water quality analysis further analyzes heavy metal pollutants in the reservoir water. Detect lead, mercury, and cadmium heavy metals in the water through high-performance liquid chromatography or atomic absorption spectrometry. Based on the test results, adjust the ratio of microbial agents to increase the degradation or adsorption capacity of these heavy metals.

[0046] f. During the floc degradation process, intermediate products or by-products are generated, which are monitored through water sampling and analysis. Physical filtration, chemical neutralization or further biodegradation methods are used to treat the by-products to prevent secondary pollution;

[0047] g. After the degradation process is completed, a comprehensive water quality assessment should be conducted to confirm the floc degradation effect and the degree of water quality improvement. A detailed project evaluation report should be prepared, recording the changes in various parameters, degradation efficiency, and ecological impact during the degradation process. This report will serve as a basis for subsequent optimization and reference. By introducing microclimate control technologies such as regular aeration, temperature and humidity control, and ecological restoration measures such as plant planting and the introduction of aquatic animals, the overall balance of the reservoir ecosystem can be further adjusted to ensure that the long-term application of microbial agents will not cause ecological degradation or imbalance, and maintain the diversity and self-purification capacity of the water body. In addition to evaluating the floc degradation effect, the water quality assessment process should also include a comprehensive assessment of other ecological indicators in the reservoir, such as dissolved oxygen, nitrogen-to-phosphorus ratio, and plankton count, an ecological health indicator, to ensure that the use of microbial agents will not lead to the homogenization of the reservoir ecosystem. The assessment results will be used to regularly adjust the application strategy of microbial agents and optimize the water quality of the reservoir in combination with ecological restoration measures.

[0048] h. Through automated control systems and regular monitoring, abnormal environmental parameters or sudden problems during the degradation process can be discovered in a timely manner. According to the preset emergency plan, rapid adjustments to the dosage, suspension of addition or other remedial measures can be taken to ensure the safety and stability of the degradation process.

[0049] Example 2: This example involved the administration of a microbial agent and floc degradation experiment in a typical reservoir. The reservoir water contained high concentrations of organic matter and flocs. Analysis revealed that the primary pollutants in the water were organic matter (e.g., plant debris, animal carcasses, etc.) and the flocs produced by the decomposition of these organic matter.

[0050] Reservoir water quality analysis:

[0051] Organic matter concentration: about 150 mg / L.

[0052] Floc quantity: about 3,000 mg / L.

[0053] pH value: 7.4.

[0054] Dissolved oxygen: 6.2mg / L.

[0055] Temperature: 26℃.

[0056] Microbial community: Through high-throughput sequencing technology analysis, it was found that the main bacteria in the water body were Pseudomonas, Bacillus, sulfate-reducing bacteria, and methanogens, but the overall number was relatively low.

[0057] Microbial agent formula:

[0058] Based on the above water quality analysis, the composition of the microbial agent was optimized and the following ratio was selected:

[0059] Aerobic strains: 40% of the total bacterial agent

[0060] Pseudomonas: 20%

[0061] Bacillus: 20%

[0062] Anaerobic strains: 30% of the total bacterial agent

[0063] Methanogens: 15%

[0064] Sulfate-reducing bacteria: 15%

[0065] Auxiliary microorganisms: 15% of the total amount of microbial agents

[0066] Fungal species: 10%

[0067] Enzyme-producing bacteria: 5%

[0068] Nutritional additives: 10% of the total amount of microbial agent

[0069] Amino Acid Mix: 4%

[0070] Potassium dihydrogen phosphate: 3%

[0071] Iron, zinc, magnesium: 3% in total

[0072] Protective agent: 5% of the total amount of microbial agent

[0073] Glycerin: 3%

[0074] Sucrose: 2%

[0075] Carrier material: 10% of the total amount of the microbial agent

[0076] Bentonite: 5%

[0077] Activated carbon: 5%

[0078] Preparation of microbial agents:

[0079] Aerobic bacteria, anaerobic bacteria and auxiliary microbial strains are cultured under sterile conditions for fermentation amplification.

[0080] The ingredients are mixed according to the above ratio and freeze-dried to ensure the activity and stability of the microorganisms.

[0081] Microbial agents are stored in sealed packaging using food-grade moisture-proof and anti-oxidation materials.

[0082] Delivery plan:

[0083] Determination of the release amount:

[0084] The dosage of the inoculant is determined based on the reservoir's water volume and floc count. The total reservoir volume is 500,000 cubic meters, and the floc count is 3,000 mg / L.

[0085] The recommended dosage of microbial agents is 1-5 kg ​​per cubic meter of water. Therefore, the recommended dosage is:

[0086] Minimum delivery volume: 500,000m 3 ×1kg / m 3 =500,000kg

[0087] Maximum delivery volume: 500,000m 3 ×5kg / m 3 =2,500,000kg

[0088] The median amount released is: 1,500,000 kg.

[0089] Delivery method:

[0090] Through the automated dosing system, the microbial agent is added in stages and batches. In the initial stage, 1,000 kg of microbial agent can be added, and then gradually increased to 1,500,000 kg to ensure the uniform distribution and degradation effect of the microbial agent in the reservoir water.

[0091] Microbial monitoring and regulation:

[0092] Multiple microbial distribution monitoring points are set up in the reservoir, and data are collected in real time through a wireless sensor network.

[0093] According to real-time data, the dosage of microbial agents is adjusted to ensure the uniform distribution and degradation effect of microorganisms, while avoiding ecological imbalance of water bodies caused by excessive addition.

[0094] Monitoring and data feedback:

[0095] Temperature sensors, pH sensors and dissolved oxygen sensors are installed in the reservoir to monitor water environment changes in real time.

[0096] An automated control system is used to adjust the aeration equipment, pH adjustment equipment and dosing equipment according to environmental data, maintaining the water temperature between 20°C and 30°C, the pH value between 6.5 and 8.5, and the dissolved oxygen concentration above 5mg / L to ensure optimal activity of microorganisms.

[0097] Water quality monitoring and adjustment:

[0098] Collect water samples regularly, analyze water quality changes, and monitor the progress of floc degradation.

[0099] High-throughput sequencing technology is used to analyze the microbial community structure in water bodies to ensure the stability of the microbial community and avoid over-reliance on a single bacterial species leading to ecological imbalance.

[0100] According to water quality monitoring data, the addition ratio and amount of microbial agents should be adjusted in a timely manner, especially adaptive adjustments should be made to heavy metal pollutants in water (such as lead, mercury, and cadmium).

[0101] Treatment of by-products during degradation:

[0102] The intermediate and by-products produced during the floc degradation process are monitored through regular water sampling and analysis.

[0103] The by-products produced are treated by physical filtration, chemical neutralization or further biodegradation to prevent secondary pollution.

[0104] Degradation effect evaluation:

[0105] Degradation effect: The floc degradation effect was confirmed through water quality assessment, and the floc removal rate was expected to be 60% to 80%.

[0106] Water quality improvement: The organic matter concentration in the water body is expected to decrease by 50%-70%, the dissolved oxygen concentration will be stabilized at above 5 mg / L, and the pH value will be maintained between 6.5 and 8.5.

[0107] Ecological impact: By monitoring the changes in the structure of microbial communities, we ensure that the application of microbial agents will not cause imbalance in the water ecosystem, and that the water quality and ecological environment of the reservoir remain stable.

[0108] result:

[0109] The microbial reservoir floc degradation method of this embodiment achieved remarkable results in this reservoir, achieving a floc removal rate of 70% as expected. Water quality was significantly improved, and the microbial community maintained good stability, with no signs of ecological imbalance. Long-term monitoring showed that after the addition of the microbial agent, floc degradation efficiency in the water remained consistently high, with no degradation degradation or byproduct accumulation.

[0110] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for degrading reservoir flocs based on microbial technology, characterized in that: The following steps are involved: a. Conduct a comprehensive water quality analysis of the reservoir water, including measurements of organic matter concentration, floc count, pH, dissolved oxygen content, and temperature, to determine the cause and distribution of flocs in the reservoir. High-throughput sequencing technology will be used to analyze the existing microbial community structure in the water, identify key microbial species and their functions, and optimize the formulation of subsequent microbial agents. b. Cultivate aerobic bacteria, anaerobic bacteria and auxiliary microbial strains under sterile conditions, and amplify each strain through fermentation to ensure its activity and degradation ability. Mix the ingredients according to the following mass percentages: Aerobic strains, accounting for 40% of the total inoculum, including 20% ​​of Pseudomonas and 20% of Bacillus; Anaerobic bacteria, accounting for 30% of the total bacterial agent, including 15% of methanogens and 15% of sulfate-reducing bacteria; Auxiliary microorganisms, accounting for 15% of the total amount of the inoculant, including 10% of fungi and 5% of enzyme-producing bacteria; Nutritional additives, accounting for 10% of the total inoculum, include 4% amino acid mixture, 3% potassium dihydrogen phosphate, and 3% iron, zinc, and magnesium in total; Protective agent, accounting for 5% of the total amount of the inoculant, including 3% glycerol and 2% sucrose; Carrier material, accounting for 10% of the total amount of the microbial agent, including 5% bentonite and 5% activated carbon; The above ingredients are fully mixed under sterile conditions so that the microorganisms and nutrients are evenly distributed on the carrier material, and then freeze-dried to extend the shelf life of the microbial agent and facilitate transportation and storage; c. Determine the dosage of the microbial agent based on the water organic matter concentration, floc count, and water volume obtained in the pretreatment and assessment steps, and evenly distribute the prepared microbial agent in the reservoir water to ensure uniform distribution and effective contact of the microorganisms in the water; d. Install temperature sensors, pH sensors and dissolved oxygen sensors in the reservoir to monitor water environment parameters in real time through an automated control system; e. Regularly collect water samples and use high-throughput sequencing technology and water quality analysis methods to monitor the microbial community structure, residual flocculent matter, and organic matter degradation rate. Based on the monitoring results, adjust the dosage of microbial agents and the strain ratio in a timely manner to avoid significant changes in the microbial community structure and maintain the ecological balance of the reservoir; f. During the floc degradation process, intermediate products or by-products are generated, which are monitored through water sampling and analysis. Physical filtration, chemical neutralization or further biodegradation methods are used to treat the by-products to prevent secondary pollution; g. After the degradation process is completed, conduct a comprehensive water quality assessment to confirm the floc degradation effect and the degree of water quality improvement. Prepare a detailed project evaluation report, recording the changes in various parameters during the degradation process, degradation efficiency and ecological impact, as a basis for subsequent optimization and reference; h. Through automated control systems and regular monitoring, abnormal environmental parameters or sudden problems during the degradation process can be discovered in a timely manner. According to the preset emergency plan, rapid adjustments to the dosage, suspension of addition or other remedial measures can be taken to ensure the safety and stability of the degradation process.

2. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The auxiliary microorganisms include fungi and enzyme-producing bacteria, which are used to enhance the degradation efficiency of flocs by decomposing complex organic polymers and secreting multiple enzymes. The protective agent is selected from glycerol and sucrose, which is used to improve the survival rate and adaptability of microbial agents in water bodies.

3. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The dosage of the microbial agent is adjusted according to the total volume of the reservoir water body and the number of flocs. The dosage of the microbial agent is 1 kg to 5 kg per cubic meter of water body, and is added in stages and batches through an automated dosing device to ensure uniform distribution of microorganisms and not cause ecological imbalance in the water body.

4. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The microbial agent is stored in sealed packaging to prevent the external environment from affecting the activity of the agent. The sealed packaging material is food-grade moisture-proof and oxidation-resistant material.

5. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: During the addition of the microbial agent, multiple microbial distribution monitoring points are set up in the reservoir, and data is transmitted to the control center in real time through a wireless sensor network, thereby achieving accurate addition of microorganisms and position optimization.

6. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The enzyme-producing bacteria in the microbial agent include β-glucosidase, lipase and protease, wherein β-glucosidase is used to decompose cellulose substances, lipase decomposes fat pollutants, and protease is used to degrade protein organic matter in water bodies.

7. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The water body temperature is between 20° C. and 30° C., the pH value is between 6.5 and 8.5, and the dissolved oxygen concentration is above 5 mg / L to maintain the optimal activity of microorganisms.

8. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The water quality analysis further analyzes the heavy metal pollutants in the reservoir water, detects the lead, mercury and cadmium heavy metals in the water by high performance liquid chromatography or atomic absorption spectrometry, and adjusts the ratio of microbial agents according to the test results to increase the degradation or adsorption capacity of these heavy metals.

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