Fetal-derived atherosclerosis susceptibility model and construction method and application thereof

CN122056256APending Publication Date: 2026-05-19CENT SOUTH UNIV
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Patent Information

Application Number
CN202610326029.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-17
Publication Date
2026-05-19

AI Technical Summary

Technical Problem

Existing animal models of atherosclerosis cannot simulate fetal characteristics caused by adverse intrauterine environmental exposure, which limits in-depth exploration of the developmental origin mechanism of atherosclerosis and research on early life intervention strategies.

Method used

A fetal atherosclerosis susceptibility model was established by subcutaneously injecting dexamethasone into healthy ApoE-/- mice at 16-17 days of gestation, combined with feeding F1 generation mice with a high-fat, high-cholesterol diet. Plaque morphology and biochemical indicators were then examined.

Benefits of technology

A fetal susceptibility model for atherosclerosis was successfully constructed, which significantly aggravated atherosclerotic lesions in offspring. This provides a research tool with more typical pathological features and faster disease progression, supporting research on the mechanisms of atherosclerosis progression and drug evaluation.

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Abstract

The invention relates to the technical field of animal model construction, in particular to a fetal-derived atherosclerosis susceptibility model and a construction method and application thereof. The construction method of the fetal-derived atherosclerosis susceptibility model comprises the following steps that S1, a healthy fertilized ApoE- / -mouse is selected, dexamethasone subcutaneous injection is conducted twice a day after 16-17 days of pregnancy, the injection amount of dexamethasone every time is 0.6-0.8 mg / kg of the weight of a fertilized female mouse, and the fertilized female mouse freely diets; s2, the fertilized female rats produce naturally to obtain an F1 generation, weaning is conducted 3-4 weeks after birth, male and female newborn rats are separated into cages, normal diet feeding continues to be conducted till 6-7 weeks after birth, and then the newborn rats are fed with high-fat and high-cholesterol feed for 18-19 weeks; the fetal-derived atherosclerosis susceptibility model is obtained. The dexamethasone is injected for a specific time, a fetal-derived atherosclerosis susceptible model is successfully constructed, and atherosclerosis lesion of the model shows a remarkable aggravating effect.
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