Method for preparing broad spectrum genetically engineered nano vaccine for malignant tumour
A genetic engineering and nano-vaccine technology, applied in the field of applied genetic engineering and vaccines, can solve the problems of the core part - the unsatisfactory antigen, the unsatisfactory immune adjuvant, and the lack of modern engineering technology penetration and development in the preparation process of tumor vaccine, and achieve the goal of killing Strong tumor effect, easy to use effect
Inactive Publication Date: 2005-10-05
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology
In recent years, countries all over the world are actively developing tumor vaccines. Many tumor vaccines have entered clinical trials. Ideal, and MHC limitation, and for a long time, the tumor vaccine preparation process lacks the penetration and development of modern engineering technology (such as nanotechnology), resulting in some difficult problems, such as the targeting of vaccines, the effective presentation of antigens, Sustained-release issues for vaccines, etc. have yet to be resolved
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[0008] The present invention will be further described in detail below in conjunction with the accompanying drawings and the embodiments made by the inventors based on the above-mentioned technical solutions.
[0009] Concrete implementation steps of the present invention:
[0010] 1. Cloning of tumor-specific consensus antigen genes
[0011] 1.1. Cloning and sequencing of MAGE-1 gene
[0012] 1.1.1. Sequence of human MAGE-1 gene (Homo sapiens)
[0013] 1 atgtctcttg agcagaggag tctgcactgc aagcctgagg aagcccttga ggcccaacaa
[0014] 61 gaggccctgg gcctggtgtg tgtgcaggct gccgcctcct cctcctctcc tctggtcctg
[0015] 121 ggcaccctgg aggaggtgcc cactgctggg tcaacagatc ctccccagag tcctcaggga
[0016] 181 gcctccgcct ttcccactac catcaacttc actcgacaga ggcaacccag tgagggttcc
[0017] 241 agcagccgtg aagaggaggg gccaagcacc tcttgtatcc tggagtcctt gttccgagca
[0018] 301 gtaatcacta agaaggtggc tgatttggtt ggttttctgc tcctcaaata tcgagccagg
[0019] 361 gagccagtca caaaggcaga aatgctggag agtgtcatca aaaatta...
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The present invention method of malignant tumor broad-spectrum gene engineering nano vaccine adopts the following steps: adopting tumor specific common antigen gene melanin tumor antigen-I (MAGE-1) and biological immunoadjuvant heat shock protein 70(Hsp70) to make gene connection to form fusion gene (MAGE-1-Hsp70), utilizing colibacillus expression, separation and purification to obtain MAGE-1-Hsp70 fusion protein, and utilizing denaturation and renaturation to obtain fusion protein with high activity, combining said fusion protein and superantigen staphylococcal enterotoxin A to form complex antigenic compound; preparing nano liposome, covering the complex antigenic compound, utilizing molecular connection of N-hydroxysuccinimide ester and Hsp70 to form N-Hsp70, and inserting it into outer layer of nano liposome phospholipid membrane.
Description
technical field [0001] The invention belongs to applied genetic engineering technology, relates to a vaccine, in particular to a preparation method of a genetically engineered nano vaccine for malignant tumors of liver cancer, gastric cancer and breast cancer. Background technique [0002] In my country, the incidence of malignant tumors ranks first in urban areas and second in rural areas, seriously threatening people's health and lives. The active immunotherapy of tumor patients with tumor vaccines can stimulate and enhance the immune rejection of tumors, which provides a new way for the treatment of tumors. In recent years, countries all over the world are actively developing tumor vaccines, and many tumor vaccines have entered clinical trials. Ideal, and MHC limitation, and for a long time, the tumor vaccine preparation process lacks the penetration and development of modern engineering technology (such as nanotechnology), resulting in some difficult problems, such as t...
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IPC IPC(8): A61K9/107A61K9/127A61K39/00A61K39/39A61K48/00A61P35/00C12N15/62
Inventor 隋延仿叶菁吴道澄李增山孙玉静陈广生曲萍
Owner FOURTH MILITARY MEDICAL UNIVERSITY