A kit for improving gene editing efficiency and application thereof

By leveraging the synergistic effect of T5 exonuclease and histone deacetylase inhibitors, the problem of low editing efficiency of the CRISPR/Cas12i complex in eukaryotes has been solved, enabling efficient gene editing in different species and genes.

CN122189095APending Publication Date: 2026-06-12INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Filing Date
2026-04-01
Publication Date
2026-06-12

AI Technical Summary

⚠Technical Problem

In eukaryotes, the gene editing efficiency of the CRISPR/Cas12i complex is limited by the spatial structure of chromatin, resulting in low editing efficiency, especially in large livestock.

⚗Method used

The editing activity of Cas12iMax was enhanced by the synergistic effect of T5 exonuclease and histone deacetylase inhibitors (such as trichostatin A and ronostatin).

🎯Benefits of technology

It significantly improves the gene editing efficiency of Cas12iMax in different species such as cattle, humans, and pigs, enhances the editing effect of multiple genes, and expands the scope of application.

✦ Generated by Eureka AI based on patent content.
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Abstract

The application provides a kit for improving gene editing efficiency and application thereof, and belongs to the technical field of gene editing. The kit comprises T5 exonuclease and / or histone deacetylase inhibitor. The application provides a method for improving CRISPR / Cas12iMax gene editing efficiency, that is, T5 exonuclease and / or histone deacetylase (HDAC) inhibitor is combined with Cas12iMax to perform gene editing on multiple species cells to be edited, so that the gene editing efficiency is greatly improved. The method helps to accelerate the breeding process of excellent varieties in the field of agricultural biotechnology, provides new tools and strategies for genetic improvement of cattle and other domestic animals, and has important scientific value and application prospect.
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