一种吸收比率 / 荧光淬灭双模式一氧化氮荧光探针及其制备方法和应用

By developing a dual-mode nitric oxide fluorescent probe with absorption ratio and fluorescence quenching, the problems of complex sample pretreatment, poor anti-interference ability and low spatiotemporal resolution in NO detection in existing technologies have been solved, and high-sensitivity self-calibrated quantitative detection in complex environments has been achieved.

CN122404356APending Publication Date: 2026-07-17ZHENGZHOU NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHENGZHOU NORMAL UNIV
Filing Date
2026-06-03
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing NO detection methods suffer from problems such as complex sample pretreatment, poor anti-interference ability, and low spatiotemporal resolution in real-world environments or biological samples. Traditional single-mode fluorescent probes are difficult to achieve accurate quantification in complex systems.

Method used

A dual-mode nitric oxide fluorescent probe based on absorption ratio and fluorescence quenching was developed. By utilizing the ratio of the two absorption peaks in the UV-Vis absorption spectrum and the fluorescence quenching characteristics in the fluorescence emission spectrum, self-calibrated quantitative detection can be achieved.

Benefits of technology

It achieves highly sensitive and interference-resistant quantitative detection of NO in complex environmental matrices, suitable for rapid on-site screening and precise laboratory quantification, and reduces preparation costs.

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Abstract

本发明属于化学检测领域,涉及一种吸收比率 / 荧光淬灭双模式一氧化氮荧光探针及其制备方法和应用;荧光探针NOP1的结构式如下:。该制备方法合成步骤简单,只需通过一步反应,产率较高。在紫外‑可见吸收光谱中,探针识别NO前后两个吸收峰的吸光度比值发生明显变化,呈现比率型响应,同时溶液颜色发生肉眼可辨的颜色变化。荧光光谱测试结果表明:探针NOP1对NO具有专一的荧光识别性能,并能克服其他常见分析物的干扰,具有较宽泛的pH值适用范围。该探针对NO的识别具有较好的灵敏度,其最低检出限为0.87 μM。探针NOP1在公共安全和生物领域具有广阔的应用前景。
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Citation Information

Patent Citations

  • Nitric oxide fluorescent probe as well as preparation method and use method thereof

    CN119874566A