Unlock instant, AI-driven research and patent intelligence for your innovation.

Collagen gel artificial skin substitute

A collagen gel and dermis technology, applied in the field of bioengineering, can solve the problems of no such products on the market or apply for patents, and achieve the effect of promoting the formation of granulation tissue, with simple technology and simple preparation method.

Inactive Publication Date: 2006-05-10
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There is no such product on the market or patent application in China; there are similar products abroad, but no artificial leather with the same composition is on the market or patent application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagen gel artificial skin substitute
  • Collagen gel artificial skin substitute

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] Take human fetal dermal fibroblasts of the 2nd-3rd generation in the exponential growth phase, digest with 0.25% trypsin solution, pipette, centrifuge, suspend in DMEM-10% fetal bovine serum culture medium, count on a hemocytometer, and adjust the cell concentration to 5.0×10 6 / ml, stand-by; prepare 0.2% collagen dispersion with 0.01M acetic acid solution (sterilized) and bovine tendon type I collagen (sterilized) extracted in this laboratory, take 40ml for stand-by; prepare concentrated DMEM (10 times) culture solution 5ml, 0.3M glutamine solution 0.5ml, 1M sodium bicarbonate solution 1.8ml, fetal bovine serum 6ml; the above 4 kinds of solutions and 40ml collagen dispersion were dropped into 100ml sterile cone Add 3ml of fibroblast suspension to a shaped bottle (placed on ice, that is, under ice bath conditions), mix quickly, and immediately drop the collagen-cell suspension into a 12-well plate with a pipette, each well 3ml, 37°C incubator for 3-5 minutes, after the...

example 2

[0030] Take human fetal dermal fibroblasts of the 2nd-3rd generation in the exponential growth phase, digest with 0.25% trypsin solution for 0.5-1 minute, add 2ml of fetal bovine serum to stop the digestion, pipette, disperse the cells into a single-cell suspension, and suspend In DMEM-10% fetal calf serum culture medium, count on a hemocytometer and adjust the cell concentration to 5.0×10 6 / ml, stand-by; prepare 0.2% collagen dispersion with 0.01M acetic acid solution (sterilized) and bovine tendon type I collagen (sterilized) extracted in this laboratory, take 80ml for stand-by; prepare concentrated DMEM (×10) 10ml of culture medium, 1ml of 0.3M glutamine solution, 3.6ml of 1M sodium bicarbonate solution, 12ml of fetal bovine serum; drop the above 4 kinds of solutions and 80ml of collagen dispersion into a 200ml sterile conical Add 6ml of human fetal dermal fibroblast suspension to the bottle (placed on ice), mix quickly, and immediately drop the collagen-cell suspension in...

example 3

[0032] Take human fetal dermal fibroblasts of the 2nd-3rd generation in the exponential growth phase, digest with 0.25% trypsin solution, pipette into a single cell suspension, suspend in DMEM-10% fetal bovine serum culture medium, and count with a hemocytometer , adjust the cell concentration to 5.0 x 10 7 / ml, stand-by; use 0.01M acetic acid solution (sterilized) and bovine tendon type I collagen (sterilized) extracted in this laboratory to prepare 0.2% collagen dispersion, get 20ml stand-by; prepare concentrated 2.5ml of DMEM (×10) culture solution, 0.25ml of 0.3M glutamine solution, 1ml of 8.4% sodium bicarbonate solution, 3ml of fetal bovine serum; drip the above four kinds of solutions and 40ml of collagen dispersion into 50ml of Add 1ml of human fetal dermal fibroblast suspension to the Erlenmeyer flask (placed on ice), mix quickly, and immediately drop the collagen-cell suspension into the 24-well culture plate with a pipette. 1ml, 37°C incubator for 3-5 minutes, afte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to bioengineering technology, in particular to collagen gel artificial dermis and a preparation method thereof. Including 80 parts of bovine tendon collagen dispersion, 10 parts of DMEM culture solution, 1 part of glutamine solution, 6 parts of sodium bicarbonate solution, 12 parts of fetal bovine serum, after mixing, add 6 parts of human fetal dermal fibroblast suspension, The collagen gel artificial dermis can be made by culturing in vitro. The preparation method of the collagen gel artificial dermis of the present invention is simple in technology, its composition and physical and chemical properties are close to normal dermis, has potential clinical application prospects, can be directly applied to transplantation to patients with skin defects, and promotes the formation of granulation tissue at the wound and the migration of adjacent epithelium. Promotes the repair and regeneration of skin defects.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to collagen gel artificial dermis and a preparation method thereof. Background technique [0002] There is no such product on the market or patent application in China; there are similar products abroad, but no artificial leather with the same composition is on the market or patent application. The technologies closely related to the present invention are collagen sponge artificial dermis, decellularized allogeneic dermis, and artificial material dermis substitutes. Collagen sponge artificial dermis requires cross-linking agents, which are toxic to the body after transplantation; acellular allogeneic dermis and artificial material dermis substitutes still have a certain gap in structure and composition from normal skin. Contents of the invention [0003] The object of the present invention is to provide a collagen gel artificial dermis and a preparation method thereof. The invention ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60
Inventor 张其清马东瑞
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI