Sheep lamb increasing essence
A technology of goat and androstenedione, which is applied in the fields of peptide/protein components, diseases, antibody medical components, etc., can solve the problems of high price of human serum albumin, high production cost, and economical uneconomical, etc., and achieve shortening of genetic breeding process, increase the lambing rate, and the effect of simple and easy operation
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[0021] A kind of goat lambsin, the chemical structure of its compound is androstenedione-17-carboxymethyloxime-bovine serum albumin. Use mineral oil to emulsify the adjuvant and antigen to form a two-phase emulsion of oil-in-water-in-water. The adjuvant ingredients are No. 25 white oil, Span 85 (sorbitan trioleate), and Tween 80 (polyethylene sorbitan monooleate).
[0022] Firstly, after freeze-drying the synthesized androstenedione-17-carboxymethyloxime-bovine serum albumin, the antigen aqueous solution is prepared according to production requirements; and then an emulsifier (adjuvant) is used. The emulsifier is to mix No. 25 white oil, Span 85 and Tween 80 in a ratio of 1:0.1:0.25, mix and stir in the colloid mill, and then slowly add the prepared antigen aqueous solution to the colloid mill. 1 part of emulsifier, 2 parts of antigen solution. The speed of the colloid mill is 5000 rpm.
[0023] The test method for the success or failure of emulsification:
[0024] ① Injec...
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