Preparation method of detection filter membrane used for bacteria and virus sampling in air

Abstract

The invention discloses a preparation method of a composite membrane used for sampling and detecting microorganisms in the air. Place the supporting porous membrane on a horizontal glass plate, adjust the height of the membrane-making knife, and move the knife holder at a speed of 5-6 cm per minute, so that the membrane-making liquid is evenly distributed on the surface of the porous membrane to form a composite membrane skin layer, and the reaction temperature is 20 ~30℃, humidity 20~50%, pressure is negative pressure below atmospheric pressure, then heat treatment at 40~80℃ for 1~3 hours, and at the same time, composite membranes with different pore sizes can be prepared by adjusting the concentration of chitosan membrane-making liquid . The macromolecular substances in the composite membrane can maintain the activity of the entrapped microorganisms to a certain extent. Compared with other filter membranes, the composite membrane can reduce the formation of filter cake, and the flux is significantly increased compared with other filter membranes with the same pore size under the same test conditions. At the same time, the macromolecule coating solution used has biological activity, which can help microorganisms to maintain activity to a certain extent, so that the sample can reflect the air condition more realistically, and it also improves the problem of microbial activity maintenance during microbial sampling and reduces detection errors.

Description

technical field [0001] The invention relates to a preparation method of a filter membrane used for sampling and detecting bacteria and viruses in the air. Background technique [0002] The use of membrane filtration to collect aerosol particles is the most common method for aerosol sampling. The so-called aerosol refers to a dispersion system in which solid or liquid particles are suspended in a gaseous medium. Pasteur in 1861 and Petri in 1888 began to collect microbial aerosols through filter membranes. It is a simple and inexpensive method of collecting aerosol samples. [0003] In recent years, membrane filtration has been widely used for aerosol sampling. Many materials are used for filter membrane materials, the most important filter membrane materials are fibrous filter materials and porous membrane filter materials. [0004] Most of the fiber filter materials are arranged in the direction perpendicular to the air flow, the porosity is 70-9...

AI Technical Summary

Problems solved by technology

[0010] 2. Can not make a beneficial combination of microbial action and retention efficiency

From the point of view of the filter membrane materials used in domestic filter samplers, it is difficult to better solve the above-mentioned shortcomings.

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