Kuhseng-targeting preparation and its preparing method
A technology of Sophora flavescens and preparations, which is applied in the field of Sophora flavescens targeted preparations and its preparation, which can solve the problems of drug delivery, lack of targeting, and lack of liver targeting of drugs, so as to change the peak and valley phenomenon, poor therapeutic effect, The effect of increasing drug safety
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Embodiment 1
[0035] Embodiment 1: Sophora flavescens targeting poly(n-butyl cyanoacrylate) nanosphere injection (by making 1000ml)
[0036] prescription:
[0037] Matrine: 200g
[0038] n-Butyl cyanoacrylate: 100ml
[0039] Sodium metabisulfite: 25g
[0040] Dextran-70: 100g
[0041] Preparation:
[0042]Mix 200g of matrine, 25g of sodium pyrosulfite, and 100g of dextran-70 in a beaker, add water for injection to dissolve, adjust the pH value to 2 with 0.1mol / L dilute hydrochloric acid, transfer it into the beaker, and slowly add cyano group under magnetic stirring n-butyl acrylate, after stirring at room temperature for 3 hours, adjust the pH value to 7 with 0.1mol / L dilute sodium hydroxide, and use G 3 Suction filtration through a vertical fused glass funnel, and add water for injection to 1000ml. Sophora flavescens targeting polycyanoacrylate n-butyl nanosphere injection.
Embodiment 2
[0043] Example 2: Sophora flavescens targeting polylactic acid nanospheres
[0044] prescription:
[0045] Oxymatrine: 100g
[0046] Polylactic acid: 50ml
[0047] Dichloromethane: 500ml
[0048] Polyvinyl alcohol: 50ml
[0049] Preparation:
[0050] First, polylactic acid was dissolved in volatile organic solvent dichloromethane to prepare a 10% solution, and 100 mg of oxymatrine was suspended in the solution. Then the obtained mixture is suspended in the stirring 1% polyvinyl alcohol solution, stirring constantly, the solvent is volatilized from the formed micro-droplets, centrifuged to obtain solid drug-loaded microspheres, collected microspheres and used Wash with deionized water, filter, and dry under vacuum at room temperature.
Embodiment 3
[0051] Embodiment 3: oxidized Sophora flavescens targeted liposome injection (by making 1000ml)
[0052] prescription:
[0053] Oxymatrine: 200g
[0054] Soy lecithin: 50g
[0055] Cholesterol: 125g
[0056] Methanol and chloroform: 50ml
[0057] Preparation:
[0058] Dissolve the prescribed amount of soybean lecithin, cholesterol, matrine, etc. in a mixed solvent of 50ml methanol and chloroform, pour it into a rotary thin film evaporator, and remove organic solvents such as methanol and chloroform under vacuum and reduced pressure at a temperature of 35°C , so that it forms a uniform dry film on the bottle wall; add 50ml of phosphate buffer, shake to form large multilayered liposomes, put it in a high-speed tissue masher, 12000r / min×2min×4 times, After standing, pass through a 0.45 μm microporous membrane to obtain a milky white uniform Sophora flavescens targeted liposome injection.
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