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Methods for determining oils in seeds

A technology for seeds and oil particles, which is used in measuring devices, food testing, scattering characteristics measurement, etc., and can solve the problems of time-consuming gravimetric analysis, inapplicability of gravimetric analysis, and low sensitivity.

Inactive Publication Date: 2006-11-22
MONSANTO CO (MONSANTO CY)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gravimetric analysis is not suitable for small numbers of samples (including small numbers of seeds and seeds with little oil content), because the oil content of these samples is below the minimum sensitivity of the technique
In addition, using gravimetric analysis is time-consuming and not amenable to high-throughput automation

Method used

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  • Methods for determining oils in seeds
  • Methods for determining oils in seeds
  • Methods for determining oils in seeds

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0079] The detector can be calibrated with soybean oil at a known concentration of 0.1-3.5 mg oil / ml solvent. Calibration results such as figure 2 shown. figure 2 is the curve of the chromatographic peak area (Y axis) - the amount of extracted oil contained in the solvent (X axis). Soy samples were crushed with a Tecator Cyclotec 1093 sample grinder (Fos Tecator, P.O. Box 70, S-26321 Hoeganaes, Sweden). Add 20 mg of crushed seeds to a microcentrifuge tube. 1 ml of hexane was added to the tube and the tube was stirred on a rotary wheel for 5 minutes at room temperature. The tube was then centrifuged for 5 minutes in a microcentrifuge. The supernatant was transferred to an HPLC autosampler vial and injected into the HPLC machine. Add 5 ml of hexane containing the extracted oil. The HPLC machine was a Hewlett-Packard 1090 with Micra NPS, 33 x 4.6 mm, 1.5 μplus Guard column. The second solvent is 10% / 90% isopropanol / hexane, the flow rate is 0.5ml / min, the column temperatu...

example 2

[0081] Figure 4-6 Results are shown comparing the oil content of the present invention (light scattering or LS) with the conventional technique of Accelerated Solvent Extraction (ASE). The oil content determination conditions of LS are the same as those in Example 1, and ASE is carried out by conventional operation. Figure 4 The percentage of oil determined by LS and ASE is shown, and the ratio of oil content (LS / ASE) has been determined by each method on 12 different samples. like Figure 4 As shown, the ratio of oil content is obviously between 0.80-0.90. Figure 5 The same ratio of oil content is shown for the 10 sorghum samples. Also, the ratio of oil content is within a narrow range, apparently between 0.60-0.65. Image 6 The same oil content ratios are shown for corn. In this case, the ratio of oil content is clearly between 0.7-0.8.

example 3

[0083] Figure 7 The reproducibility of one example of the method of the invention was demonstrated. like Figure 7 As shown, the oil content of 10 corn samples measured during 10 identical runs ranged from 2.75 to 3.25 percent oil. The determination of the oil content of the sample is the same as in Example 1.

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Abstract

The present invention relates to methods of analyzing agricultural products. More specifically, the present invention relates to a method of analyzing the oil content of one or more seeds comprising the steps of: extracting the oil from the seeds with a solvent; evaporating the solvent in a gas stream to form particles; detecting the reflected light and measure the oil content.

Description

field of invention [0001] The invention relates to an analysis method of agricultural products. More specifically, the present invention relates to methods of analyzing the oil content of one or more seeds. Background of the invention [0002] It has long been a goal to improve techniques for analyzing agricultural products for desired traits. In order to analyze the presence of a particular quality in a sample, several methods have traditionally been used. Quantitative analysis of seed oil content is often accomplished by several conventional methods such as near infrared analysis (NIR), nuclear magnetic resonance imaging (NMR), Soxhlet extraction, accelerated solvent extraction (ASE) , microwave extraction and supercritical fluid extraction. However, these conventional methods cannot accurately discern relative or absolute levels of oil in very few seed samples. [0003] Over the past decade, near-infrared (NIR) spectroscopy has become the standard method for screening...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/03G01N15/00G01N21/47G01N21/53G01N30/74
CPCG01N15/00G01N21/47G01N21/53G01N30/74G01N33/03G01N2021/4709
Inventor D·V·文雅莫里J·A·龙R·K·达斯
Owner MONSANTO CO (MONSANTO CY)