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Agaricus blazei bacterium exopolysaccharide preparing process

A production method and a fungus cell technology are applied in the production field of edible fungal polysaccharides, can solve the problems of falsely high output of extracellular polysaccharides, problems of quality and purity, easy agglomeration, etc., so as to be beneficial to industrial application, ensure purity, and improve yield Effect

Inactive Publication Date: 2006-11-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Description
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Problems solved by technology

However, Agaricus blazei can not only produce a large amount of mycelia during the fermentation process in the culture medium, but also secrete and accumulate exopolysaccharides in the culture medium. In order to obtain this part of exopolysaccharides, we use the above-mentioned starch raw materials for cultivation Based on experiments, it was found that after high temperature sterilization, it is easy to agglomerate or ball, resulting in the formation of Agaricus blazei mycelium balls containing starch granules; In addition to the β-glucan, there is also some starch polysaccharide (raw material) that is α-glucan, which leads to a falsely high yield of exopolysaccharide, and the quality and purity become a problem

Method used

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Examples

Experimental program
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Embodiment 1

[0022] Agaricus brasiliensis strain: Agaricus brasiliensis 03, the strain was tissue isolated from Agaricus blazei fruiting bodies grown by mushroom farmers in Curitiba, Paraná, Brazil. Inoculate it on PDA (potato dextrose agar) slant medium. After incubating at 25~28℃ for 7-10 days, store it in a refrigerator at about 4℃ for later use. Inoculate the strain on PDA slant medium before use. Upper activation, that is, after 7-10 days of constant temperature culture at 25~30℃, the mycelium (without culture medium) is inoculated in a sterilized Erlenmeyer flask containing liquid seed culture medium and cultured statically for 5 days. The formula is (weight%): glucose 2%, yeast extract 0.2%, K 2 HPO 4 0.2%; MgSO 4 0.05%, the balance is water, pH6, and the culture temperature is 28~30℃. At this time, the mycelium grows white and prosperous. Use a sieve to collect the mycelium in the liquid, and remove the mycelium under aseptic conditions. Wash the bacteria three times with water, put th...

Embodiment 2

[0025] The formula of the production medium in this example is: 50 grams of sucrose, 8 grams of a 1:1 mixture of yeast extract and ammonium sulfate, K 2 HPO 4 5 grams, MgSO 4 2.5 g, the balance is water, prepared into 1000 ml of culture solution, adjusted to pH 6, and the rest of the steps from the preparation of the strain to the extraction and purification are the same as in Example 1. The extraction rate of extracellular polysaccharide in this case was 789.5 mg / L.

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Abstract

Disclosed is a method for producing polysaccharide, which has advanced the preparation to produce culture medium, and techniques of fermentation and abstraction, including manufacture of deformation, preparation of seed culture medium and inoculation, production of culture medium for inoculation, abstraction and purification of culture medium. It has increased the quality and purity of the productivity made its productivity to be 825.2mg / L; it has simple technique, low cast and can be applied in industry widely.

Description

Technical field [0001] The invention relates to the production field of edible fungal polysaccharides, in particular to a fermentation culture and extraction production method of Agaricus blazei Murrill extracellular polysaccharides. Background technique [0002] Agaricus blazei, also known as Brazilian mushroom, is a very rare medicinal and food fungus, and its scientific name is A. brasiliensis. Agaricus blazei Murrill is rich in nutrients, delicious, and has a strong almond flavor. Each 100g of Agaricus blazei dry product contains 40-45 grams of crude protein, 38-45 grams of sugar, 6-8 grams of fiber, and 3-4 grams of crude fat. , Is an edible fungus rich in protein and carbohydrates; in addition, it is also rich in vitamins, such as VB 1 0.3-0.4 mg, VB 2 3.2-3.7 mg, niacin 22-50 mg, ergosterol 100-200 mg (pro VD). The bacterium contains 18 kinds of amino acids, among which the essential amino acids of human body account for 39.7-42.8% of its total am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/04
Inventor 范雷法潘慧娟
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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