Monoclonal antibody, testing kit including the monoclonal antibody and application
A monoclonal antibody and kit technology, applied in the field of immune applications, to achieve the effects of low temperature requirements, strong specificity, and a wide range of detection samples
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] 1. Preparation of anti-chicken IgG Fc monoclonal antibody:
[0027] Refer to the method in Xue Qingshan's "Principles and Techniques of In Vitro Culture" Science Press, 2001 edition: use the chicken IgG extracted by the inventor's Microbiology and Immunology Laboratory to immunize Balb / C mice (purchased from the Experimental Animal Center of Hubei Academy of Medical Sciences) The immunization procedure is to emulsify a protein solution containing 50-100 μg of chicken IgG with an equal volume of Freund's complete adjuvant (purchased from sigma company), inject into the abdominal cavity of mice, and then strengthen it every ten days, and replace it with incomplete adjuvant emulsification (sigma). Finally, three days before the fusion, (preferably more than 4 weeks after the last immunization), intraperitoneal booster immunization, double the amount of antigen, without adjuvant. At the time of fusion, take a Balb / C mouse that has undergone the final booster immunization, ...
Embodiment 2
[0031] Preparation of Chicken Avian Influenza Virus NP Protein:
[0032] For the molecular biology methods used, please refer to the literature: Sambrook J, Fritsch E F, Maniartis T (translated by Jin Dongyan, Li Mengfeng, etc.). Molecular Cloning Experiment Guide. Second Edition, Beijing Science Press, 1992 method provided in.
[0033] 1. Cloning and sequencing of NP gene
[0034] The NP gene involved in the present invention was cloned by the applicant himself, and the sequence of the gene has been registered in the GeneBank database with the accession number AY788915. The sequence has 97% homology with known sequences. The specific method of cloning and sequencing of NP gene is: a strain A / chicken / China / HSS2004 (H9N2) [GeneBank accession number is AY788915, the isolation and For identification, see literature: Li Zili et al. Diagnosis of avian influenza in Hubei Province and isolation and preliminary identification of virus strains, Chinese Journal of Preventive Veterina...
Embodiment 3
[0040] Preparation of rabbit anti-mouse IgG antibody:
[0041] Refer to editor-in-chief such as He Zhaoyang, "Animal Immunology Experimental Technology" Changchun: Jilin Science and Technology Press, the method in 2002: Utilize the Balb / C mouse IgG that the inventor's place extracts from the microbiology and immunity laboratory to immunize healthy rabbits, prepare high Specific and high-titer rabbit anti-mouse IgG hyperimmune serum, the hyperimmune serum is crudely extracted by saturated ammonium sulfate precipitation, and high-purity rabbit anti-mouse IgG antibody is obtained after passing through the G-200 column. Use this antibody as a quality control line.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More