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Ethanol feedback control flow added high density fermentation method for yeast and use thereof

A technology of ethanol concentration and high-density culture is applied in the field of high-density culture of yeast cells and the production of yeast metabolites to achieve the effect of improving the control effect

Inactive Publication Date: 2007-03-07
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using an ethanol concentration monitor to monitor the ethanol concentration in the fermentation broth on-line, which is applied to the high-density fermentation of yeast cells, has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: high-density culture Candida utilis produces yeast cell

[0033] Use a 30L general-purpose fermenter for fermentation. Prepare 9L fermentation medium (medium formula: glucose 60g / L, yeast powder 15g / L, wort 40g / L, diammonium hydrogen phosphate 10g / L, magnesium sulfate 5g / L, peptone 10g / L, dipotassium hydrogen phosphate 20g / L, potassium dihydrogen phosphate 12g / L, Zn 2+ 10ppm, Fe 2+ 、Cu 2+ , Mn 2+ Each 6ppm), the real tank is sterilized. After the medium temperature drops to 30°C, inoculate, adjust the ventilation ratio to 1.2VVM, the stirring speed to 400 rpm, and start fermentation. Add glucose aqueous solution with a concentration of 650g / L from 11.5 hours. Fermentation proceeds to 15.4 hours, and the ethanol concentration drops to 6g / L. At this time, the flow acceleration rate is adjusted according to the ethanol concentration. When the ethanol concentration rises, the flow acceleration rate is reduced. , when the ethanol concentration drops, incr...

Embodiment 2

[0034] Example 2: High-density cultivation of Candida tropicalis production yeast cells

[0035] Use a 50L general-purpose fermenter for fermentation. Prepare 18L fermentation medium (medium formula: glucose 73g / L, yeast powder 9g / L, wort 28g / L, diammonium hydrogen phosphate 9g / L, magnesium sulfate 6g / L, peptone 8g / L, dipotassium hydrogen phosphate 16g / L, potassium dihydrogen phosphate 18g / L, Zn 2+ 13ppm, Fe 2+ 、Cu 2+ , Mn 2+ Each 4ppm), the real tank is sterilized. After the medium temperature drops to 30°C, inoculate, adjust the ventilation ratio to 1.2VVM, the stirring speed to 300 rpm, and start fermentation. Add glucose aqueous solution with a concentration of 650g / L from 12 hours. Fermentation proceeds to 17.1 hours, and the ethanol concentration drops to 8g / L. At this time, the flow acceleration rate is adjusted according to the ethanol concentration. When the ethanol concentration rises, the flow acceleration rate is reduced. , when the ethanol concentration drop...

Embodiment 3

[0036] Example 3: High-density culture of Saccharomyces cerevisiae cells

[0037] Use a 150L general-purpose fermenter for fermentation. Prepare 50L fermentation medium (media formula: glucose 80g / L, yeast powder 18g / L, wort 40g / L, diammonium hydrogen phosphate 9g / L, magnesium sulfate 3g / L, peptone 10g / L, dipotassium hydrogen phosphate 12g / L, potassium dihydrogen phosphate 12g / L, Zn 2+ 10ppm, Fe 2+ 、Cu 2+ , Mn 2+ Each 6ppm), the real tank is sterilized. After the medium temperature drops to 30°C, inoculate, adjust the ventilation ratio to 1.1VVM, the stirring speed to 200 rpm, and start fermentation. Add glucose aqueous solution with a concentration of 650g / L from 13 hours. Fermentation proceeds to 18 hours, and the ethanol concentration drops to 6g / L. At this time, adjust the flow acceleration rate according to the ethanol concentration. When the ethanol concentration rises, reduce the flow acceleration rate. , when the ethanol concentration drops, increase the flow acc...

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PUM

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Abstract

The present invention is alcohol feedback controlled high density yeast fermentation method and its application, and relates to method of high density method of culturing yeast cells and producing yeast metabolite. Alcohol concentration monitoring instrument is used in on-line monitoring of alcohol concentration in the fermented liquid and controlling the optimal alcohol concentration. The saccharomycetes adopted is saccharomyces cerevisiae, protein generating candidan or candida Tropicalis. The optimal culture medium consists of glucose, yeast powder, wheat juice, diammonium biphosphate, magnesium sulfate, peptone, diportasium biphosphate, portasium dihydrogen phosphate, Zn ion, Fe ion, Cu ion and Mn ion. By means of the alcohol concentration feedback control, the present invention culture high density yeast cells and produces the target product.

Description

(1) Technical field [0001] The invention relates to a method for cultivating yeast cells at high density and producing yeast metabolites. It is used to control feeding through ethanol concentration feedback, and is used for high-density cultivation of yeast cells and production of target products through yeast cells. (2) Background technology [0002] Yeast is one of the main carrier microorganisms for the fermentation of biological products, and is also a host cell often used in recombinant genetic engineering products. In order to improve the economy of the production process and the market competitiveness of products, biotechnology researchers continue to create more economical and effective methods, optimize the production process, and achieve high productivity. High-density culture of yeast cells is an important means to improve the production level of yeast expression products. Due to the limitations of the physiological characteristics of microbial cells and factors...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/865C12R1/72C12P21/02C12P33/00
Inventor 谭天伟温少红王峥尚飞
Owner BEIJING UNIV OF CHEM TECH