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Substrates, preparation and use

A substrate, methyl technology, applied in the field of substrate, preparation and application, can solve difficult microarray and other problems

Inactive Publication Date: 2007-04-18
CYTEC SURFACE SPECIALTIES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Difficulty obtaining microarrays with increased functional and / or active site density and / or graft density

Method used

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  • Substrates, preparation and use
  • Substrates, preparation and use
  • Substrates, preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Synthesis of hydroxy-functional urethane acrylates

[0131]A 444 g amount of preheated 5-isocyanato-1-isocyanatomethyl-1,3,3-trimethylcyclohexane (also known as IPDI, commercially available from Degussa-Huels, Germany ) into a 2-liter four-necked round-bottomed flask equipped with a stirrer, thermometer, water-cooled condenser and dropping funnel. The mixture was heated at 45°C and then 0.37 g of dibutyltin dilaurate (also known as DBTL, commercially available from Akcros) was added as catalyst. 232 g of hydroxyethyl acrylate and 0.925 g of hydroquinone monomethyl ether were added slowly from the dropping funnel while maintaining the temperature of the reaction mixture at a maximum of 65°C. The mixture was kept at this temperature for one hour until I NCO Reached 2.96meq / g. Then add 250g of two-trimethylolpropane (I OH 898mgKOH / g), while further heating the reactant at 65°C until 1 NCO dropped below 0.05meq / g. The oligomer was cooled at 40°C and diluted with 927 g...

Embodiment 2

[0133] Synthesis of Polyols

[0134] Mix 1,6-hexanediol (1,144.2 g) and adipic acid (1,135.6 g) in a three-liter reactor equipped with stirrer, packed column, condenser, thermometer and inert gas inlet (both can be commercially available from BASF) with DBTL catalyst (0.02 g). The reactor is flushed with an inert gas and the reactants are heated to a temperature of 195° C. to 200° C. while removing water produced by the esterification. The reaction was continued for 5 hours until I H + is 5mg KOH / g and I OH is 117 mg KOH / g, thus obtaining a polyol product with a M of 1000 n and a final I of 112 mg KOH / g OH . This polyol was used directly in the formulation described below.

[0135] Substrate Formulation and Coating

[0136] Substrate

[0137] The functionalized substrates of the present invention were prepared from commercial compact discs (uncoated plain polycarbonate CDs commercially available from ISP, Belgium) and coated with a wire-wound bar coater as described he...

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Abstract

There is disclosed a process for preparing a substrate having thereon sites reactive with the reactive groups of a molecular probe, the process comprising the steps of applying to the substrate surface a material comprising one or more reactive sites having an activated ethylenically unsaturated double bond of the formula I. Wherein, if n=0, m=1, X3 is carbon and X1 is nitrogen, the bond between them being triple, and R4 does not exist; if n=1, m=1, X3 is carbon and X1 is oxygen or sulphur, the bond between them being double, and X2 is oxygen or sulphur or nitrogen substituted by an organo substituent; if n=1, m=1 or 2, X3 is sulphur and X1 is oxygen, the bond between them being double, and X2 is nitrogen substituted by an organo substituent; R1, R2, each independently represent hydrogen, hydroxyl or organo group, R3 and R4 each independently represent hydrogen or organo group, R1 and R2 being not linked to R4, and, in a further step, of covalently binding the reactive group of the molecular probe to the activated ethylenically unsaturated double bond of the reactive sites having formula I. Preferred activated unsaturated moiet(ies) comprises unsaturated ester moiet(ies) such as (meth)acrylate moiet(ies). Preferred probes comprise amino groups thereon. Preferred strong links are covalent bonds. Preferred linking reactions are Michael addition reactions. The substrate having probes deposed thereon may be used to interact with a target. Other aspects of the invention relate to substrates and multi-reactive systems (such as micro-arrays) so prepared; uses thereof; and products and information obtained therefrom.

Description

technical field [0001] The present invention relates to improvements in active substrates that can be used to form functionalized substrates for use in multi-reaction systems incorporating probes that, for example, interact with target molecules. Aspects of the invention relate to methods of chemically modifying the surface of a substrate to covalently bind molecules (e.g., molecular probes) thereon; substrates obtained and / or obtainable from said method; using said substrate to prepare Further methods of multi-reaction systems; multi-reaction systems obtained or obtainable by said method; use of said multi-reaction systems in analysis and / or synthesis; products synthesized directly and / or indirectly using said multi-reaction systems ; and / or use of information obtained from said multiple reaction system. [0002] In embodiments herein, the multiple reaction system may be a microarray (e.g., a DNA microarray - also known as a biochip); the probes may be DNA sequences and / or o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J19/00C12Q1/68B01L3/00G01N33/543C08F20/02C12N15/09C08F291/00C08G85/00C40B40/06
CPCB01J2219/00605B01J2219/00722B01J2219/00585G01N33/54386B01J2219/00626B01J19/0046C40B40/06B01J2219/00608B01J2219/00659Y10T428/31504B01J2219/00707B01J2219/0072B01J2219/00527B01J2219/0061B01J2219/00637B01J2219/00596G01N33/54366G01N33/54393B01J19/00
Inventor L·林德肯斯M·泰勒曼斯S·凯普勒J·利麦科勒B·德贝克尔
Owner CYTEC SURFACE SPECIALTIES INC