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Luminescence detecting device, luminescence detecting mocroarray plate

A luminescence detection and microarray technology, which is applied in the field of gene or protein determination, can solve the problems of poor measurement accuracy and short life time, and achieve the effect of reducing the interference of luminescence, reducing the loss, and reducing the loss of luminescence

Inactive Publication Date: 2007-08-08
HITACHI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to chemiluminescence, the lifetime of luminescence is also short, and measurement must be performed in a short time after adding a luminescent substrate, and the measurement accuracy of the micro-area scanning method after the luminescence reaction starts is not good.

Method used

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  • Luminescence detecting device, luminescence detecting mocroarray plate
  • Luminescence detecting device, luminescence detecting mocroarray plate
  • Luminescence detecting device, luminescence detecting mocroarray plate

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Embodiment Construction

[0026] Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings.

[0027] 1 is an explanatory diagram showing an example of a microarray plate for luminescence detection used in the present invention, FIG. 1(a) is a perspective view, and FIG. 1(b) is a cross-sectional view along line A-A of FIG. 1(a).

[0028]In the microarray plate 10 shown in FIG. 1, a shallow rectangular recess (sample introduction area) 11 is formed on a part of the surface of a glass plate such as a slide, and a plurality of circles in an array are formed on the bottom surface of the rectangular recess. Shaped pits (tiny pits) 12. Each of the plurality of minute wells 12 has a flat bottom, and a part of the bottom serves as a reaction molecule immobilization region where the target nucleic acid and the hybridized nucleic acid probe in the sample are immobilized. The sample introduction area 11 is provided for simultaneously dispensing one sample cont...

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Abstract

A luminescence microarray plate suitable for use in luminescent reaction, and a luminescence detecting device utilizing the microarray plate. A partition wall is provided around each microarray minute reaction region to prevent the entry of surrounding luminescence. Luminescence from each minute region is detected by a photo conducting guide. The amount of entry of surrounding luminescence can be reduced and improvements in accuracy and sensitivity can be achieved.

Description

technical field [0001] The invention relates to a method for luminescent detection of nucleic acid or protein in a biological sample, in particular to a technique for measuring genes or proteins in micro-sample. Background technique [0002] In recent years, the number of assays of genes and proteins has been increasing, and automation and simplification of assays are strongly demanded. However, the measurement sample is expensive, so it is desirable to reduce the amount of measurement used. Furthermore, in such samples, the concentration of the substance to be measured is often low, and high-sensitivity measurement is desired. Luminescent detection methods, known as chemiluminescence or bioluminescence, can increase sensitivity by more than a single digit compared to fluorescence or absorbance methods. In the past, microplates or optical elements were often used for luminescence detection methods (Japanese Patent Application Laid-Open No. 6-225752, Japanese Patent Laid-Op...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/76G01N33/566G01N33/53C12Q1/68
CPCG01N2201/0846G01N21/76G01N21/253
Inventor 保田健二高桥智
Owner HITACHI LTD