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Transfer factor specific for SARS virus and method for preparing same

A transfer factor and specific technology, applied in antiviral agents, antiviral immunoglobulins, pharmaceutical formulations, etc., can solve problems such as drugs that have not been developed for SARS

Inactive Publication Date: 2004-11-17
陆家海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, people have not yet developed a drug that can effectively treat SARS

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 SARS inactivated vaccine

[0034] The bottom area is 275cm 2 Cultivate VeroE6 (National Institute of Virology, China) in cell flasks. After the cells grow into a dense monolayer, wash the cells with serum-free medium for 3 times, add 100ml of serum-free medium, and inoculate F69 and Z 2 -Z 3 SARS-Cov virus strain, when cytopathic to 75%-100%, titrate virus, TCID 50 is log10×10 7 / ml. Freeze and thaw the culture flask 3 times in a -20°C refrigerator, shake well and place in a 2-8°C refrigerator overnight, inactivate with 0.4% formaldehyde for 24 hours, 5000rpm×30min, discard the precipitate, and use the supernatant as a SARS inactivated vaccine.

Embodiment 2

[0035] Embodiment 2 immunization program (SARS inactivated vaccine immunization alone)

[0036]17 healthy castrated boars (body weight 50Kg, fine breed pig farm in Guangzhou City) use embodiment SARS inactivated vaccine (TCID50Log107 / ml). A total of 4 times, the first time was Freund's complete adjuvant + SARS inactivated vaccine; the second time was Freund's incomplete adjuvant + SARS inactivated vaccine, and the third and fourth times were simple SARS inactivated vaccine immunization; Dosage 3×TCID50; Inoculation method Multi-point intramuscular injection on the back. The time interval of immunization is 0d, 15d, 21d, 40d. 1-3ml of whole blood was collected from the ear vein, and the intervals of serum collection were 0d, 5d, 7d, 10d, 15d, 21d, 28d, 35d and 42d. The serum was then centrifuged and stored at -20°C for future use.

[0037] Embodiment 2. Immunization program (SARS inactivated vaccine and influenza vaccine combined immunization)

[0038] 13 healthy cast...

Embodiment 3

[0039] Example 3. Test of immune effect Detection of neutralizing antibody

[0040] The immunized pigs in Examples 1 and 2 were carried out according to the method for evaluating and detecting the immune effect of inactivated vaccines.

[0041] 1. Virus dilution Dilute the titrated virus to 100TCID 50 / 25ul.

[0042] 2. Animal serum The serum of pigs immunized with SARS virus in different periods was collected in the

[0043] Start from 1:10 on a sterile 96-well plate, and serially dilute to 1:

[0044] 10240, 2 wells for each dilution, and collect the pre-immunization at the same time

[0045] Various animals were used as controls.

[0046] 3. Neutralization test Add 25ul 100TCID to the above serum dilution well 50 / 25ul

[0047] For Z2-Y3 virus application solution, shake gently and place at 36°C 5% CO 2

[0048] Culture in an incubator, and set normal cell control and virus titer back at the same t...

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Abstract

The invention discloses a transfer factor specific for SARS virus and method for preparing same, wherein the process according to the invention comprises the steps of, deactivation of SARS virus, immunizing animals, extracting specific transfer factors, and refining and purification of specific transfer factors. The transfer factors specific for SARS virus can be prepared by using deactivation SARS virus as antigen, or by using the combination of deactivation SARS viruses and influenza viruses inactivated vaccine as antigens.

Description

technical field [0001] The present invention relates to a "SARS" virus-specific transfer factor and a preparation method thereof, in particular, the present invention relates to a "SARS" virus-specific transfer factor prepared from an inactivated "SARS" virus as an antigen and its Preparation. Background technique [0002] The SARS virus is the culprit of the SARS disease that has been raging in my country and even in the world this year. Its spread has seriously affected people's normal life order. The SARS virus is a brand new coronavirus, not a recent variant of a known coronavirus. Coronavirus is an RNA virus. Since March 2003, the gene sequence of the SARS coronavirus has been reported successively at home and abroad. The virus contains a complete 5' end sequence, coding sequence and 3' end sequence. The composition structure of the virus is similar to that of other coronaviruses, including multiple open reading frames (ORFs), which encode the virus's polymerase 1a,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/26A61K35/407A61K38/02A61K39/215A61K39/395C07K2/00C07K16/10C12N7/04
Inventor 陆家海王一飞潘兴华
Owner 陆家海