Unlock instant, AI-driven research and patent intelligence for your innovation.

Process for enrishing bacteria of producing polyketone kinds compounds

A technology of polyketide compounds and bacteria, which is applied in the direction of biochemical equipment and methods, measurement/testing of bacteria and microorganisms, etc., can solve problems such as microbial enrichment methods that have not yet been seen, and achieve the effect of strong pertinence and simple operation

Inactive Publication Date: 2005-03-23
OCEAN UNIV OF CHINA
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing microbial enrichment technologies are mainly based on microbial species (such as fungi, actinomycetes, etc.) Method for Enrichment of Microorganisms of Specific Compounds

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] 1. Pretreatment of environmental samples:

[0007] If sea water, lake water or river water is used, impurities can be removed by filtering with a 200-mesh nylon sieve, the filtrate is filtered with a 0.22 μm microporous membrane, and the filter cake is suspended in PBS buffer to make the cell concentration 10 5 pieces / ml; if marine animal and plant samples are used, cut the marine animal and plant samples into small pieces, soak them in PBS, oscillate ultrasonically, filter with a 200-mesh nylon mesh to remove impurities, filter the filtrate with a 0.22 μm microporous membrane, and filter the cake Suspend with PBS buffer to make the cell concentration 10 6 samples / ml; if soil or sea mud samples are used, add an appropriate amount of PBS to the soil or sea mud, oscillate and mix well, take the supernatant after standing for 10 minutes, continue to add PBS to the sedimentation, and repeat the above operation three times. The supernatant was filtered with a 200-mesh nylon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention is a method of enriching polyketone compound-producing bacteria, and its characteristic: making environmental sample into liquid mixed suspension, filtering and centrifuging to collect microbes, then making them into bacterial suspension by buffer liquor, then making in-situ hybridization on fluorescent-labeled oligonucleotide and the bacterial suspension, fluorescently labeling the bacterial able to produce the polyketone compounds, separating the microbes from the bacterial suspension after hybridization by flow type cell apparatus, thus separating fluorescent-labeled bacteria from the bacterial suspension. It can enrich the bacteria producing polyketone compounds in the environment, such as soil, river water, lake water, sea water, sea mud, especially enriching large number of uncultured bacteria in the environmental sample, having strong pertinence, operating simplicity, speediness, sensitivity and high performance.

Description

technical field [0001] The invention relates to a microorganism enrichment method, in particular to a method for enriching polyketide-producing bacteria and a fluorescent labeling probe used therefor. Background technique [0002] Polyketides are a large class of structurally diverse natural products with important medical value, including macrolides, tetracyclines, anthracyclines, polyethers and many other antibiotics. The annual sales of drugs derived from polyketides amount to 8 billion US dollars in the world. Polyketides are mainly derived from the secondary metabolites of microorganisms, plants and other organisms. Due to the small size of microorganisms, fast reproduction speed, and mature large-scale cultivation technology, they have become important resources for finding new polyketides. The traditional method is to first isolate microorganisms, and then conduct activity screening to isolate active secondary metabolites. Whether this method can obtain polyketides i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/00C12N1/20C12Q1/02C12Q1/68
Inventor 朱天骄李静
Owner OCEAN UNIV OF CHINA