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Manual sectioning method for tree trunk

A technique of freehand slicing and slicing, which is applied in the direction of material analysis, measuring devices, and instruments by optical means, can solve problems such as difficulties, and achieve the effect of simple method, easy to master, and good slicing effect.

Inactive Publication Date: 2005-05-11
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to slice the cadre diseased tissue with rough skin, especially the cadre diseased tissue that has dried up in the next year.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Tools: double-sided or single-sided blade; microscope; glass slide; coverslip; brush or inoculation needle.

[0014] Floating agent: water

[0015] Specific operation: The thumb and forefinger of the left hand hold the cadre disease specimen to be cut steadily, so that the fruiting body on the lesion to be cut faces the operator, and the right hand holds the blade to slice directly on the fruiting body of the lesion. It should be noted that the posture of holding the diseased specimens of cadres is in principle to allow the two hands to move freely, but not to be close to the body or pressed on the table. When slicing, the blade should form an angle of about 90 degrees with the specimen to be cut, with the edge facing inward, and cut from top to bottom. When the blade moves from the front left to the rear right, do not stop halfway, so that the cut slices can reflect the pathogenicity more completely. Morphological characteristics.

[0016] In order to obtain better s...

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PUM

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Abstract

A sectioning method includes holding disease specimen to be cut on tree trunk steadily, making subentity to be cut on scab face to operator and carrying out sectioning directly on subentity of tree trunk disease scab with slicer held in hand.

Description

technical field [0001] The invention relates to identification technology of forest tree diseases, in particular to a free-hand slicing method for tree cadre diseases. Background technique [0002] Freehand sectioning is a commonly used technique in forest tree disease identification. To identify a forest disease, in addition to observing the external symptoms of the disease, it is important to observe the morphological characteristics of the pathogen with a microscope to identify the disease, which requires the tissue section of the disease specimen. [0003] There are two commonly used methods for sectioning: one is paraffin sectioning; the other is freehand sectioning. The paraffin section method is to use a microtome to cut the pre-treated materials such as selection, fixation, dehydration, transparency, wax dipping, and embedding into thin slices. Its production effect is better, and its storage time is long, but the procedure is complicated, the chemical treatment is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04G01N21/00
Inventor 赵敏
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI