Application and formulation of dammarane type gen-seng low valence alcohol derivative
A dammarane-type, derivative technology, applied in the field of medicine
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Embodiment 1
[0042] Pharmacokinetic study of the preparation of the present invention
[0043] Experimental drug: take the tablet of the present invention (with Panaxadiol Potassium Succinate as raw material) and powder injection of the present invention (with Panaxatriol Sodium Succinate as raw material) as an example to carry out experiments
[0044] Experimental animals: 4 healthy male dogs, weighing 18-21kg.
[0045] Experimental method: get 2 dogs, the tablet of the present invention is given by intragastric administration according to the dosage of 6 mg / kg, blood is taken from a side forelimb vein at different times, heparin anticoagulant plasma is prepared, and panaxadiol is determined by high performance liquid chromatography Content, the blood concentration data at different times were automatically fitted on the Great Wall 0520CH computer (with the MCPKP program compiled by Xia Wenjiang, Lanzhou Institute of Traditional Chinese Veterinary Medicine, Chinese Academy of Agricultural...
Embodiment 2
[0067] Acute Toxicity Test
[0068] Experimental animals: healthy mice 18-22g, half male and half male.
[0069] Experimental drugs: tablets of the present invention, water injections, infusions, and powder injections (provided by the laboratory of some companies in Guangdong Tianzhijiao Drug Development)
[0070] Normal saline (provided by the laboratory of some companies in Guangdong Tianzhijiao drug development)
[0071] Experimental method: with tablet of the present invention according to 10 times, 20 times, 50 times of normal dosage, i.e. intragastric administration of 300mg / kg, 600mg / kg, 1500mg / kg, administration 3 times every day, continuously for 7 days, observe small Rat death situation, record data, calculate the LD50 of tablet of the present invention, see table 3; Similarly, inject preparation of the present invention according to 10 times, 20 times, 50 times of normal dosage, i.e. tail vein administration 15mg / kg, 30mg / kg, 75mg / kg, administered once a day, con...
Embodiment 3
[0095] Long-Term Toxicology Studies
[0096] Experimental animals: 8-week-old SD rats, weighing 100-120 g.
[0097] Experimental drugs: tablets of the present invention, water injections, infusions, and powder injections (provided by the laboratory of some companies in Guangdong Tianzhijiao Drug Development)
[0098] Normal saline (provided by the laboratory of some companies in Guangdong Tianzhijiao drug development)
[0099] Instrument: automatic biochemical analyzer (Hitachi 7600, JAPAN)
[0100] Experimental method: Take rats, take oral tablets and administer them by intragastric administration according to the dosage of 21 mg / kg (calculated according to the safe dosage for human administration), and administer the normal saline group by intragastric administration with equal volume and different volumes, 3 times a day , continuously for 16 weeks, observe the rats, without a case of death, the rats are dissected, and the tablet of the present invention is calculated to a...
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