SARS coronary virus full virus vaccine
A technology of SARS virus and whole virus, which is applied in the field of severe acute respiratory syndrome virus vaccine and its preparation, and can solve the problems of strong toxicity and strong infectious ability
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Embodiment 1
[0039] Preparation of Vaccine Using Early SARS Virus
[0040] The SARS early virus used in this embodiment is the GZ-02 virus strain, its sequence is preserved in GenBank, and the accession number is AY39556. The virus strain was purchased from the Chinese Center for Disease Control and Prevention (CDC).
[0041] Compared with TOR2, this virus strain mainly has six attenuating mutations in its nucleotide sequence: 9404→C, 17564→G, 21721→A, 22222→C, 23823→G, 27827→C.
[0042] Clinically, patients infected with this virus strain showed mild symptoms. At the same time, the infectivity is low. Therefore, this virus has good properties as an attenuated vaccine.
[0043] The virus strain was cultured in Vero, the supernatant was taken, and the purified virus was obtained by immunoaffinity chromatography. It is formulated into a vaccine with conventional adjuvants.
Embodiment 2
[0045] Evaluation of Immunized Animals with Early SARS Virus Vaccine
[0046] Rhesus macaques were immunized with the vaccine prepared in Example 1 according to conventional immunization methods.
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