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Skin cell with anti-inflammatory activity, and its preparing method and use for skin infective wound surface

A skin cell, active technology, applied in biochemical equipment and methods, medical preparations containing active ingredients, skin diseases, etc., can solve the problems of poor seed cell activity, lack of anti-infection ability, etc. The effect of reducing the infection rate of transplants

Inactive Publication Date: 2005-10-05
TIANJIN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the poor activity of the seed cells themselves and the lack of anti-infection ability in the existing skin transplantation process, and provide a kind of skin cells with good proliferative activity and anti-infection activity and anti-inflammatory activity and its preparation method and the Application of Cells in Skin Infected Wounds

Method used

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  • Skin cell with anti-inflammatory activity, and its preparing method and use for skin infective wound surface
  • Skin cell with anti-inflammatory activity, and its preparing method and use for skin infective wound surface
  • Skin cell with anti-inflammatory activity, and its preparing method and use for skin infective wound surface

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Embodiment Construction

[0024] The present invention adopts conventional aseptic operation technique, takes normal mammalian or human skin, thickness 1mm~3mm, removes the hair on skin surface and subcutaneous connective tissue, washes with Hank's liquid containing penicillin 10U / ml, streptomycin 10μg / ml for 3 Second-rate. Trim the skin tissue into a size of 1mm×3mm, enter the first step of enzyme digestion: add 5-10 times the amount of 0.01-0.25% Dispase II solution, and digest in a refrigerator at 4°C for 8-24 hours. Digestion was terminated with a small amount of fetal calf serum; the epidermis and dermis were separated after washing with Hank's solution. The second step of enzymatic digestion: put the obtained epidermis into 0.01-0.25% trypsin solution, and digest in a shaker incubator at 37°C for 1-5 hours. Fetal calf serum was added to terminate the digestion, and the cell suspension was filtered through a 100-mesh steel mesh to obtain a single-cell suspension of keratinocytes. Put the obtaine...

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Abstract

This invention discloses a skin cell with anti-inflammatory reactivity, its facture and the utilization in skin infected surface. It adopts two-step enzyme digestion method to separate the skins of animals or humans, and the separated epidermis cells and fibroblast cells are co-cultured in the anti-inflammatory liquid containing 50-90% MEM basic culture liquid, 5-40% serum and 0.5-10% Chinese medicine distilling liquid, the intensity of the cells is 1X10 to the power 4 -1X10 to the power 6 / ml, and makes the cells have anti-inflammatory and functional reactivity. The reactivated cells are planted on the infected surface and they will grow well on the surface to promote the formation of granulation tissue and speed the restoring procedure of the infected surface.

Description

technical field [0001] The invention relates to a medical preparation derived from skin, in particular to a skin cell with anti-inflammatory activity, a preparation method thereof and the application of the cell in infected wounds of the skin. Background technique [0002] Skin defects and skin ulcers are increasing due to trauma, burns, infection and diabetes. Accelerating the repair of large-area skin defects and skin ulcers and reducing the disability rate are important issues in the field of surgery. With the development of modern medical technology, great progress has been made in skin transplantation techniques such as autologous and allogeneic skin transplantation, allogeneic or heterogeneous cell-free dermal substitutes, and synthetic substitutes. Especially in recent years, the intersection of life science and engineering has given birth to tissue engineering. The establishment and development of tissue engineering has opened up a new way for the repair of skin de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/36A61L27/60A61P17/02C12N5/08
Inventor 李秀兰白仁骁张杨师宜健静蔼晨
Owner TIANJIN HOSPITAL