Nucleic acid recovery chip and nucleic acid recovery device
A recovery device, nucleic acid technology, applied in biochemical cleaning devices, enzymology/microbiology devices, tissue cell/virus culture devices, etc., can solve the problem of lack of nucleic acid component separation, analysis, and lack of progress in research methods.
Inactive Publication Date: 2005-10-05
安田贤二
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Problems solved by technology
[0006] In order to analyze the structure of living organisms or their activities, etc., as mentioned above, there is a lack of separation and analysis of nucleic acid components in specific regions in specific cellular states, and little progress has been made in the research of methods for this.
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[0050] The application of the present invention has the characteristics as described above, and the embodiments thereof will be described below.
[0051] First, an example of the basic configuration of the nucleic acid recovery chip according to the present invention will be described using the example shown in FIG. 1 . In the nucleic acid recovery chip 100 , an optically absorbing thin film layer 102 such as a deposited layer of chromium is disposed on an optically transparent substrate 101 such as slide glass. When observing with transmitted light, it is preferable that the film thickness of the thin film layer 102 is such that it does not completely absorb light, and is so thin that there is no mottling. For example, when chromium is deposited with a film thickness of 50 Å, it transmits about 70% of the light in the visible light range, which does not hinder its use in the application of the present invention. In addition, any metal of Ti, V, Fe, Co, Ni, Mo, and W may be u...
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Nucleic acid recovery device, which is laminated on a glass substrate transparent to a specific wavelength and has an absorption region for the specific wavelength; a device that applies a potential to the conductive region; a device that binds nucleic acid to the region; A container for accommodating cells in the above-mentioned area; a device for culturing cells in the container; a device for observing the above-mentioned cells; by irradiating the above-mentioned area with a beam of light of a specific wavelength to locally heat the area near the beam, the heat will bind the cells A device that locally dissociates and recovers nucleic acid components in the above region. In order to clarify the distribution of nucleic acid components in cells in a specific state or the distribution of nucleic acid components in each cell in a tissue cell group, this device can selectively separate nucleic acid components in a specific region of each cell in a specific cell state ,Recycle.
Description
technical field [0001] The present application relates to a nucleic acid recovery chip and a nucleic acid recovery device that observe cells while culturing, and selectively separate and recover nucleic acid components in a specific region of the cells when the cells are in a specific state. Background technique [0002] In recent years, the development of methods and devices for the efficient and high-precision separation and analysis of DNA, nucleic acid, and protein, which are biologically related samples, has rapidly progressed. [0003] For example, the well-known DNA chip is a method for detecting a specific nucleotide sequence using micropatterning technology and DNA complementarity. In this method, oligonucleotides with various sequences are patterned on the array in advance on the substrate, the binding position of the DNA sample on the substrate is detected, and the sequence of the DNA in the sample is analyzed. In addition, it is well known that the PCR reaction ...
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IPC IPC(8): G01N1/28C07H21/00C12M1/00C12M1/42C12M3/00C12N15/09
CPCC07H21/00C12M35/02
Inventor 安田贤二一木隆范冈野和宣
Owner 安田贤二