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Prepn process of high-concentration photosynthesis bacterial prepn

A photosynthetic bacteria preparation and technology of photosynthetic bacteria, applied in the direction of bacteria, etc., can solve the problems affecting the quality of the bacteria agent, the cumbersome operation process, and the difficulty in guaranteeing the quality, and achieve the effects of shortening the production time, increasing the yield of bacteria, and improving production efficiency

Inactive Publication Date: 2005-12-14
郭伟光
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  • Application Information

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Problems solved by technology

What's more is to carry out regular stirring in the cultivation process (as the Chinese patent whose publication number is CN1400303A), it can be seen that the focus of attention of most photosynthetic bacteria preparation methods is to focus on carbon source, light intensity, temperature, oxygen content and The control of pH value is carried out by adding carbon source, oxygenation, stirring and other methods to carry out the production of bacterial agents. One of these methods is to add carbon source and fill in oxygen during production. The operation process is cumbersome and requires certain equipment; The second is that it is easy to infect miscellaneous bacteria, which affects the quality of the bacterial agent; the third is that the culture time is long, the bacterial content is low, and the quality is difficult to guarantee

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Prepare bacterial strains: photosynthetic bacteria adopt Rhodopseudomonas capsulata and Rhodopseudomonas palustris Rps.Palustris, respectively culture these two strains and mix them at a volume ratio of 1:1 to obtain a mixed strain;

[0017] 2. Preparation of culture medium: Weigh 1.5kg of yeast extract, 0.5kg of potassium dihydrogen phosphate, 0.3kg of magnesium sulfate, 2.5kg of sodium acetate, and 0.8kg of fulvic acid, dissolve them in 1000kg of water, add 1kg of papaya extract, and adjust The pH value is 7.0;

[0018] 3. Cultivate strains: Add 180kg of mixed strains into the culture medium, mix evenly, put it into a transparent plastic bucket, plug it with a cotton plug, and cultivate it under the conditions of temperature 32-35°C and light intensity 2000LX for 3-5 days , Shake well every 5 hours during the cultivation process, and when the concentration of viable bacteria in the bacterial solution reaches more than 3 billion / ml, it will be made into a photosynth...

Embodiment 2

[0020] 1. Prepare strains: photosynthetic bacteria adopt Rhodobacter capsulatus, Rhodobacter palustris Rps.rutila and Rhodopseudomonas globosa Rps.Globiformis. The three bacterial strains are cultured separately and mixed according to the volume ratio of 1:1:1 to obtain the mixed strain;

[0021] 2. Preparation of culture medium: Weigh 2kg of yeast extract, 0.5kg of potassium dihydrogen phosphate, 0.5kg of magnesium sulfate, 3kg of sodium acetate, and 0.5kg of fulvic acid, dissolve them in 1000kg of water, then add 1.5kg of papaya extract to adjust the pH Value is 7.0;

[0022] 3. Cultivation of strains: Add 200kg of mixed strains into the culture medium, mix evenly, put them into a transparent plastic bucket, plug them with cotton plugs, and cultivate them for 4 days at a temperature of 32-35°C and a light intensity of 2500LX. Shake once every 5 hours during the process, and when the concentration of viable bacteria in the bacterial solution reaches more than 3 billion / ml, i...

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Abstract

The preparation process of high-concentration photosynthesis bacterial preparation includes the following steps: adding fermentation promoter and papaya extracting liquid into basic photosynthesis bacterial culture medium to prepare bacteria culture liquid, adding strain in 10-20 vol% of the culture liquid, setting into transparent container, and micro-aerobic culturing at temperature of 28-35 deg.c and illumination intensity of 1500-2500 LX for 72-120 hr to prepare photosynthesis bacterial preparation with bacteria content over 3 billion / ml. The micro-aerobic culturing condition is formed by means of stopping the mouth of the culture container with sterilized cotton stopper and shaking the container once every 5-8 hr. compared with common culturing process, the present invention has simple operation, short culture period, fast growth speed, high thallus yield, stable preparation quality and certain bacteriostasis.

Description

technical field [0001] The invention relates to a preparation method of a high-concentration photosynthetic bacteria preparation. Background technique [0002] The preparation method of photosynthetic bacteria preparation is a lot, and as publication number is the method that the Chinese patent introduction of CN1104250A is: inoculate the mixed flora of two strains of more than 30% bacterial strains in bacterial culture liquid, the culture liquid after the inoculation is at temperature 26- 32°C, light intensity 3000-5000LX, pH 6-8, culture under anaerobic conditions for 48-72 hours, then put the cultured bacterial liquid in an open system under natural light, according to the remaining carbon source in the bacterial liquid Add the carbon source more than once in a timely manner, and cultivate for 72-120 hours under aerobic and aerated conditions at a temperature of 20-35°C, then collect the bacterial liquid and pack it to make a liquid microbial mixed preparation. What's mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 郭伟光
Owner 郭伟光