Halogen insect poison detection method and its detection box

Abstract

The invention discloses an Artemia virus detection testing method and box, including the steps: 1) firstly placing Artemia cysts in a light room of the special testing box to make artificial sea water incubation by light culturing for 24-48 hours, then switching the light room with a dark room, where because the hatched larvas tend to the light, they swim through micro-pores on a transverse clapboard into the light room and the dead cysts remains in the dark room, using suction tube to eliminate these wastes and completing the hatching course; 2) after injected in the light room, the liquor to be tested diffuses through these micro-pores, and finally the concentration of the tested liquor balances in the light and dark rooms; under this environment, feeding these larvas with viruses for 24 hours, switching the light room with the dark room, where the unpoisoned larvas swim through the micro-pores into the light room and the poisoned ones remain in the dark room, sucking up the positioned ones from the dark room for counting so as to obtain an accurate lethality. The invention is rapid and safe, simple but practical, and accurate and reliable, reducing the subjective factors of artificial judging and suitable for rapid, large-scale activity screening and toxicity monitoring operation.

Description

technical field [0001] The invention relates to a test method for Artemia poison detection and a test box thereof. Background technique [0002] At present, it is an internationally recognized biological detection method to use the lethality of the total number of Artemia as an index of physiological activity to conduct primary toxicity screening of food, plant extracts, medicines, water sources, etc. Its operation is simple, fast, wide coverage, easy to master technology. [0003] However, for a long time, the commonly used method is to put Artemia eggs into large test tubes, add artificial seawater, and hatch under certain conditions. The surviving larvae were poured into another large test tube with fresh artificial seawater to continue hatching to obtain the larvae needed for the experiment. Then, according to the living habits of Artemia, the number of swimming times of Artemia within 10 seconds was determined on the drip reaction plate as the poisoning threshold inde...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a test method for Artemia poison detection and its test box in order to overcome the shortcomings of many steps, time-consuming and poor accuracy in the current Artemia virus detection method

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