Method for reinforcing diphenyl polychlorisde biodegradation utilizing sucrose fat
A technology of biodegradation and polychlorinated biphenyls, applied in the field of environmental bioengineering, can solve the problems of low bioavailability, achieve the effect of promoting bioremediation rate, eliminating inhibition, and increasing mass transfer efficiency
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Embodiment 1
[0049] Screening of Bioavailable Surfactants
[0050] Anionic surfactant sodium dodecylbenzenesulfonate, nonionic surfactant OP-10, Tween20, Tween80, TritonX-100, and biosurfactant sucrose lipid were selected as carbon sources. Prepare the primary screening medium of surfactant plus mineral salt, its content is: surfactant 2g / L, KH 2 PO 4 1.7g / L, K 2 HPO 4 4.4g / L, NH 4 Cl 2.1g / L, NaCl 3.0g / L, yeast extract 0.05g / L, MgSO 4 ·7H 2 O0.195g / L, MnSO 4 ·H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.01g / L, CaCl 2 2H 2O 0.003g / L, deionized water 1L, pH 7.0-7.2. Take 1 ml of the suspension of PCBs-degrading bacteria Raoultella terrigena LY402, add them to 50 ml of synthetic medium containing different surfactants, and cultivate them in a shaker at 30°C and 150 rpm. The growth time is 0, 5 , 20, 26, 44, 57, 68, 95, and 110 hours respectively, and use a spectrophotometer to detect its absorbance value at 650nm, that is, OD 650nm , to draw the growth curve. When the culture time reaches...
Embodiment 2
[0052] Screening of surfactants capable of promoting PCB biodegradation
[0053] Using biphenyl, nonionic surfactant OP-10, Tween-20, Tween-80, Triton-100 and biosurfactant sucrose lipid as carbon sources respectively, plus minerals with the same content as the culture medium in Example 1, Six kinds of liquid synthetic media containing different surfactants were prepared, and the amount of carbon source was 2g / L.
[0054] Cultivate Raoultella terrigenaLY402 in a shaker at 30°C and 150 rpm using the above-mentioned synthetic medium, when its OD 650nm = 1.0, stop culturing and store in a refrigerator for later use.
[0055] Prepare 2mg / ml 2,3′,4,4′-tetrachlorobiphenyl-n-hexane solution, add 1ml into 5ml sterilized bottles, blow dry with nitrogen to make PCBs adsorb to the bottom of the glass bottle, the same Methods 14 bottles were prepared. The OD grown in the above-mentioned surfactant synthetic medium 650nm =1.0 of the bacterial suspension was taken out and added to the a...
Embodiment 3
[0058] Application of Sucrose Lipid in Biodegradation of PCBs Contaminated Soil
[0059] Dissolve Aroclor1242 containing more than 70 kinds of PCBs in n-hexane solution (Aroclor is the trade name of PCBs mixture, 12 represents 12 carbon atoms on the biphenyl ring, 42 represents that the chlorine content in PCBs mixture is 42%), and a certain amount The clay was mixed evenly, and n-hexane was removed by rotary evaporation, and PCBs were evenly adsorbed on the clay surface. The simulated PCBs-contaminated soil containing 2.8mg Aroclor1242 (2.8ppm) per Kg soil was prepared by this method. Cultivate Raoultella terrigena LY402400ml with sucrose lipid synthesis medium, the culture conditions and the composition of the medium are the same as in Example 2, so that the concentration reaches OD 650nm =2.0, take it out, centrifuge the bacterial suspension of 400ml, remove the supernatant, divide the bacterial sludge into 8 parts on average, add to 30 milliliters respectively without suc...
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