Unlock instant, AI-driven research and patent intelligence for your innovation.

Peptides and mixtures thereof for use in the detection of severe acute respiratory syndrome-associated coronavirus (SARS)

An immune complex and active technology, applied in the direction of viral peptides, positive-sense single-stranded RNA viruses, viruses, etc., can solve the problem of low specificity of whole virus lysate analysis

Inactive Publication Date: 2006-08-16
ADALTIS
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the same reason, the specificity of these whole virus lysate assays is also unacceptably low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Peptides and mixtures thereof for use in the detection of severe acute respiratory syndrome-associated coronavirus (SARS)
  • Peptides and mixtures thereof for use in the detection of severe acute respiratory syndrome-associated coronavirus (SARS)
  • Peptides and mixtures thereof for use in the detection of severe acute respiratory syndrome-associated coronavirus (SARS)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Embodiment 1: The peptide of the present invention detects the efficiency of anti-SARS-CoV antibody

[0126] SARS-CoV peptides corresponding to the sequences Seq ID nos: 3, 17, 22, 23, 31, 34, 37, 101, 136 and 137 were chemically synthesized and used in microtiter plate EIA ( Enzyme immunoassay) to detect anti-SARS CoV IgG antibodies.

[0127] To examine the sensitivity of these peptides, a set of 55 serum samples collected from SARS-positive patients was prepared. The serological status of all these samples was confirmed as SARS-positive by IFA (Indirect Fluorescence Assay). To test the specificity of these peptides, a set of 22 serum samples were prepared from serum pools collected from patients afflicted with other respiratory diseases in 2000 and 2001, at least two years before SARS was reported.

[0128]The results are shown in Table 1. For each peptide, the cutoff level was chosen based on the mean plus 3 standard deviations (mean+3SD) of the results obtained w...

Embodiment 2

[0130] Embodiment 2: The peptide of the present invention detects the efficiency of SARS-CoV protein

[0131] Rabbits were immunized with peptides of Seq ID no: 37 or Seq ID no: 136 (both conjugated to KLH). After 3 months, sera were collected from these rabbits and subsequently tested according to the protocol described in Protocol 6 with microplates coated with peptides of Seq ID nos: 37, 136 and 137. Addition of goat anti-IgG-peroxidase conjugate revealed the presence of specific anti-peptide antibodies in all tested antisera, but when tested with microplates coated with peptide Seq ID nos 136 or 137 There was no significant reaction when the antiserum to Seq ID no 37 was tested and when the antiserum to Seq ID no 136 was assayed on microplates coated with peptides Seq ID nos 37 or 137.

[0132] In the next set of experiments, 1 microgram (μg) of recombinant nucleocapsid (N) protein (amino acids 1-49; Biodesign International, Saco, Maine, USA) was added to the microwells a...

Embodiment 3

[0133] Embodiment 3 Peptide of the present invention detects the efficiency of SARS-CoV antigen

[0134] Microplates (1 μg / mL; 100 μL / well) were coated with recombinant N protein as described in Example 2. Next, the antiserum described in Example 2 was added to the plate and IgG bound to the coated antigen was detected following the protocol described in Protocol 6. The results obtained can be seen in Table 3. They show that antisera raised against the SARS-CoV N peptide can be used to detect the N protein of SARS-CoV.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to novel peptides and mixtures thereof useful for detecting Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV) infections in humans and animals. Therefore, the present invention provides SARS-CoV diagnostic methods and kits.

Description

field of invention [0001] The present invention relates to novel peptides and mixtures thereof effective for the detection of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection in humans and animals. Background of the invention [0002] SARS, a type of atypical pneumonia of unknown etiology, was recognized by the World Health Organization (World Health Organization) at the end of February 2003. In April 2003, scientists worldwide confirmed that a previously unrecognized coronavirus (SARS-CoV) could be the cause of SARS (Drosten et al., 2003; Ksiazek et al, 2003; Peiris et al, 2003) . [0003] To date, very few serological diagnostic tests for SARS-CoV have been developed (Drosten et al., 2003; Ksiazek et al, 2003; Peiris et al, 2003). The preferred introduction was the indirect immunofluorescence assay (IFA) (Drosten et al., 2003; Ksiazek et al., 2003; Peiris et al., 2003), followed by ELISA (Ksiazek et al., 2003; Peiris et al., 2003). The IFAs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/165
CPCC12N2770/20022C07K14/005A61P31/12
Inventor M·霍德J·M·拉克鲁瓦
Owner ADALTIS