Method for determining regenerated silk fibroin water solution concentration
A technology for regenerating silk fibroin and silk fibroin, which is applied in the field of concentration measurement of regenerated silk fibroin aqueous solution, which can solve the problems of difficulty in precise control of solution concentration, inability to quickly reflect the change of solution concentration, and long time.
Inactive Publication Date: 2006-09-06
DONGHUA UNIV
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Problems solved by technology
For the regenerated silk fibroin aqueous solution used for remolding, its concentration is generally higher (such as more than 10%). At this time, the mensuration of the solution concentration is not suitable for adopting the above-mentioned biochemical classic analysis method, such as literature [WangM, Jin HJ, KaplanD L et al.Mechanical Properties of Electrospun Silk Fibers.Macromolecules, 2004, 37 (18): 68566856-6864.] Introduce generally can only adopt traditional dry weighing method
However, the time required for this method is too long, and it cannot quickly reflect the concentration change during the concentration process of the solution, which makes it difficult to accurately control the concentration of the prepared solution.
Method used
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Embodiment 1
[0017] 1. Purify the regenerated silk fibroin
[0018] Place the peeled cocoon layer into a boiled 0.5% aqueous solution of sodium carbonate, the ratio of the cocoon layer to the aqueous solution is 1:50 (W / V), boil for 30 minutes, remove the surface sericin, repeat 2 times like this , and then fully washed with deionized water, dried to obtain degummed silk fibroin; the obtained silk fibroin was dissolved in 9.3mol / L lithium bromide aqueous solution at 30°C to obtain a 12% silk fibroin solution. The silk fibroin solution was diluted 5 times and then filtered, and placed in a dialysis bag for continuous dialysis for 3 days to remove salt ions until the conductivity of the deionized water outside the dialysis bag was basically unchanged. The dilute silk fibroin aqueous solution after dialysis is concentrated to prepare a series of regenerated silk fibroin aqueous solutions with different concentrations.
[0019] 2. Determination of the concentration of silk fibroin aqueous sol...
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Abstract
The method comprises: at constant temperature, detecting reflective index of unknown solution with a Abbe refractometer; then, obtaining the concentration by the function relation of concentration and reflective index, wherein: drying and weighting the purified solution to obtain the concentration value; then, drawing fitting function relation with the reflective index as independent variable.
Description
technical field [0001] The invention relates to a method for measuring the concentration of regenerated silk fibroin aqueous solution, and is especially suitable for measuring the concentration of regenerated silk fibroin aqueous solution with relatively high concentration. Background technique [0002] Silk has excellent properties and has been a high-grade clothing material for thousands of years. In recent decades, it has been found that its molecular structure has good biocompatibility with organisms, and it is expected to be used in non-textile fields such as artificial tendons, artificial blood vessels, and surgical sutures. In order to meet the various special needs in the non-textile field mentioned above, or in order not to be affected by changes in natural conditions such as climate and geography, and to produce silk products of different specifications and outputs according to human wishes, it is necessary to reshape the silk. In addition, it is not only a pity t...
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IPC IPC(8): G01N21/41G06F19/00G01N5/04G01N1/34
Inventor 邵惠丽李继功朱晶心张慧慧胡学超
Owner DONGHUA UNIV