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Apparatus and method for ion production enhancement

A technology of ions and ion sources, applied in the field of mass spectrometry, can solve problems such as heating and heat conduction limitations

Inactive Publication Date: 2006-09-06
AGILENT TECH INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this technique is that the heating and heat conduction of this system is limited by the material used in the capillary
Furthermore, the sensitivity of the AP-MALDI source has been limited by many factors including the geometry of the target and its position relative to the capillary, the laser beam fluence at the target surface, and the overall hydrodynamics of the system.

Method used

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  • Apparatus and method for ion production enhancement
  • Apparatus and method for ion production enhancement
  • Apparatus and method for ion production enhancement

Examples

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example 1

[0070] A Bruker Esquire-LC ion trap mass spectrometer was used for AP-MALDI studies. Modifications to the ion optics of a mass spectrometer (a splitter, dual octopole guides with partitions), the instrument's ion sampling inlet includes an ion sampling tube extension with a capillary extension that is concentric with the capillary extension pipeline. The ion sampling inlet receives a 4-10 L / min hot nitrogen flow. A laser beam (337.1 nm, 10 Hz) is delivered to the target by a 400 micron fiber through a single focusing lens. Laser power is estimated to be around 50 to 70 microjoules. Mass spectrometer data were acquired by setting the maximum capture time of the mass spectrometer scan spectrum to 300 milliseconds (3 laser shots) using ion charge control. Each spectrum is the average of 8 microscans from 400 to 2200 AMU. The matrix used was 8 mM a-cyano-4-hydroxy-benzene acrylic acid, 12% TPA, 67% water containing 1% acetic acid in 25% methanol. The matrix target was premixe...

example 2

[0072] The same target was prepared and used as above, except that the heating gas was applied to the target (ionization region) at about 100 degrees Celsius. Figure 10 The effect of applying heated gas on the target in the ionization region is shown. The graph shows the presence of a vertex (ion enhancement) at higher mass-to-charge ratios.

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Abstract

The present invention relates to an apparatus and method for use with a mass spectrometer. The ion enhancement system of the present invention is used to direct a heated gas toward ions produced by a matrix based ion source and detected by a detector. The ion enhancement system is interposed between the ion source and the detector. The analyte ions that contact the heated gas are enhanced and an increased number of ions are more easily detected by a detector. The method of the invention comprises producing analyte ions from a matrix based ion source, enhancing the analyte ions with an ion enhancement system and detecting the enhanced analyte ions with a detector.

Description

technical field [0001] The present invention relates generally to the field of mass spectrometry, and more particularly to an ion enhancement system for providing a heated gas flow to enhance analyte ions in an atmospheric pressure matrix assisted laser desorption / ionization (AP-MALDI) mass spectrometer. Background technique [0002] Most complex biochemical targets require the application of complementary multidimensional analysis tools and methods to compensate for target and matrix interferences. Proper analysis and separation are important to obtain reliable qualitative and quantitative information about the target. In this regard, mass spectrometers have been widely adopted as detectors for various separation methods. However, until recently, most spectroscopic methods provided patterns of fragmentation processes that were too complex for rapid and efficient analysis. Results have been substantially improved using atmospheric pressure ionization (API) and matrix assis...

Claims

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Application Information

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IPC IPC(8): H01J49/10H01J37/08H01J27/08H01J49/26G01N30/72
Inventor 琼鲁克·图克白剑提莫森·乔伊斯
Owner AGILENT TECH INC
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