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New vaccine adjuvant

An adjuvant and vaccine technology, applied in the field of immunology, can solve the problems of high price, toxicity and side effects, and achieve the effect of low price and induction of cellular immune response

Active Publication Date: 2007-04-11
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of toxic side effects and high price in existing vaccine adjuvants, the inventors of the present invention provided a safe, effective and stable , cheap new vaccine adjuvants

Method used

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  • New vaccine adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A. Immunity

[0019] BALB / c mice were divided into vaccine group, control group and blank group, with 25 mice in each group. The vaccine group was injected with a mixture of low molecular weight heparan acetyl sulfate and hepatitis B surface antigen, and the injection dose was 50 μg of low molecular weight heparan acetyl sulfate and 5 μg of hepatitis B surface antigen per mouse; the control group was injected with recombinant (yeast) hepatitis B vaccine containing aluminum adjuvant , at a dose of 0.4 mg Al(OH) per mouse 3 5ug HBsAg was adsorbed; the blank group was only injected with normal saline, 100μL / rat.

[0020] Immunization scheme: a total of 3 times of immunization, subcutaneous injection of BALB / c mice at 0, 1, and 6 months respectively.

[0021] B. Detection of serum anti-HBs IgG level by ELISA

[0022] At 4, 8, 16, 24, and 32 weeks after the initial immunization, the tail vein blood of the mice was collected, the serum was separated, and the serum anti-HBs...

Embodiment 2

[0046] A. Immunity

[0047] BALB / c mice were divided into vaccine group, control group and blank group, with 25 mice in each group. The vaccine group was injected with a mixture of low molecular weight heparan acetyl sulfate and hepatitis B surface antigen, and the dose was 250 μg of low molecular weight heparan acetyl sulfate and 5 μg of hepatitis B surface antigen per mouse; the control group was injected with recombinant (yeast) hepatitis B vaccine (containing aluminum adjuvant) , at a dose of 0.4 mg Al(OH) per mouse 3 5ug HBsAg was adsorbed; the blank group was only injected with normal saline, 100μL / rat.

[0048] Immunization scheme: a total of 3 times of immunization, subcutaneous injection of BALB / c mice at 0, 1, and 6 months respectively.

[0049] B. Detection of serum anti-HBs IgG level by ELISA

[0050] 4, 8, 16, 24, and 32 weeks after the initial immunization, the tail vein blood of the mice was collected, the serum was separated, and the anti-HBs IgG level in th...

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Abstract

A new type vaccine adjuvant, low molecular weight heparan sulfates, and its application in the preparation of vaccine adjuvant. The combination of the said low molecular weight heparan sulfates and vaccine can effectively enhance the humoral immune response, resemble to aluminum adjuvant and the new type vaccine adjuvant has a remarkable enhancing effect on the cellular immune response and was adapted to therapeutic vaccine.

Description

technical field [0001] The invention relates to a vaccine adjuvant, in particular to the application of low-molecular-weight heparan acetyl sulfate in preparing vaccine immune adjuvants, and belongs to the field of immunology. Background technique [0002] The basic questions of immune recognition are: ①Why does the immune system know which substances are acceptable and which substances are not acceptable? ② What is the material basis of immune recognition? ③ What are the initiating factors of the immune response? ④ Does self-tolerance exist? ……etc. The answers to the above questions have successively produced the following four different explanation modes, which cannot replace each other, but can complement each other: (1) "harmful-harmless" mode - 100 years ago, Paul Ehrlich proposed the " According to the side chain theory, the immune response is determined by the "noxious and non-noxious" properties of the antigen itself. (2) "Self-non-self" discrimination model - F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P31/12A61P1/16
Inventor 胡云章胡凝珠瞿素
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI