A hybrid rice breeding method
A technology of hybrid rice and inbred lines, applied in rice cultivation, botany equipment and methods, plant genetic improvement, etc., can solve the problems of seed production failure, self-fertilization recovery of photothermo-sensitive genic male sterile lines, and climate change differences To achieve the effect of increasing the yield of seed production, reducing the risk of seed production failure and solving the problems of seed production
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Embodiment 1
[0027] Example 1 Preparation of Pei'ai 64DS / Pei'ai 64DS' / / 9311 combination
[0028] Parents provided by photothermo-sensitive sterile gene: Yannong S, indica low-temperature sterile photothermo-sensitive male sterile line Yannong S (Xiang Pin Shen 2001 No. 313; "Hybrid Rice" 1998, 13(4): 37- 38), bred a low-temperature sterile photothermo-sensitive male sterile line approved by Hunan Province for Hengyang Agricultural Science Institute of Hunan Province.
[0029] Genomic male sterile interaction gene provides parents: 7001S (Anhui Provincial Science and Technology Progress Award 1994 Nonglin-1-005: Research on Breeding and Utilization of Japonica Photosensitive Genic Male Sterile Line 7001S; Female of Anhui Provincial Approved Variety Wandao 48 Book).
[0030] Restorer line: the male parent commonly used in hybrid rice seed production, 9311, the male parent of the two-line hybrid rice combination Liangyoupeijiu (National Approved Rice 2001001; "Jiangsu Agricultural Journal", ...
Embodiment 2
[0049] Example 2 Preparation of Yannong S / Y58-7001S' / / 9311 combination
[0050] Parents provided by the photothermosensitive sterile gene: Yannong S (Xiang Pin Shen No. 313 in 2001; "Hybrid Rice" 1998, 13(4): 37-38), bred by Hunan Hengyang Agricultural Science Institute It is a low-temperature sterile photothermo-sensitive sterile line approved by the Variety Approval Committee.
[0051] Parents provided by GMS interaction genes: 7001S (Anhui Provincial Science and Technology Progress Award 1994 Nonglin-1-005: Research on the Breeding and Utilization of Japonica Photosensitive GMS Line 7001S; Female Parent of Wandao 48 Approved Variety in Anhui Province ).
[0052] Recipient parent of GMS interaction gene: Y58S (variety right number CNA20030431.3).
[0053] Restorer line: the male parent commonly used in hybrid rice seed production, 9311, the male parent of the two-line hybrid rice combination Liangyoupeijiu (National Approved Rice 2001001; "Jiangsu Agricultural Journal", 20...
Embodiment 3
[0061] Embodiment 3 Preparation of 2610S / Y58-7001S' / / 9311 combination
[0062] 2610S is a short-day low-temperature sterile photothermo-sensitive male sterile line discovered in Xiang 125S by Sun Meiyuan of Hunan Hybrid Rice Research Center, which was directly cited in the breeding and matching of this combination.
[0063] Breeding of Y58-7001S' Reference Example 2
[0064] Refer to Example 2 for the reproductive technology of the sterile line (2610S / Y58-7001S') of the 2610S / Y58-7001S' / / 9311 combination and the seed production of the 2610S / Y58-7001S' / / 9311 hybrid seeds.
[0065] The results of observation and identification showed that the F1 of 2610S / Y58-7001S' showed stable sterility in Changsha, and was male sterile for life; the yield of 2610S / Y58-7001S' / / 9311 was high, reaching 3720 kg / ha; -7001S' / / 9311 hybrid F1 showed high yield, which was 3.9% higher than Shanyou 63, a large-area cultivation combination; the seed purity reached 99.5%.
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