Testing method for chlorophyll of phytoplankton in water

A technique for planktonic algae and a determination method, which is applied in the field of measuring algae chlorophyll a in water bodies by spectrophotometry, can solve the problems that ultramicro algae cannot be intercepted, the extraction solution is easy to lose, time-consuming and labor-intensive, etc., so as to avoid the loss of chlorophyll and heavy results. Good performance and easy operation

Inactive Publication Date: 2007-05-16
NANKAI UNIV
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Problems solved by technology

However, there are two main shortcomings of this method: the first is to use a 0.8μm filter membrane, the pore size is too large, and some ultramicroalgae

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  • Testing method for chlorophyll of phytoplankton in water

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[0022] The present invention will be further described below in conjunction with specific embodiments:

[0023] Take several water bodies of a certain lake as test water samples, filter 100ml water samples with cellulose acetate filter membrane (50cm) with a pore size of 0.45μm, put the filter membrane into a 10ml centrifuge tube with stopper, add 10ml analytically pure absolute ethanol, cover Put the stopper on, shake it several times and place it in a dark place. Take out the centrifuge tube after 6-24h, shake well and use a medical centrifuge to run at 3500r·min -1 Centrifuge, pour the supernatant into a 1cm glass cuvette, use absolute ethanol as a reference, measure the absorbance with a grating spectrophotometer at 630, 647, 664, and 750nm wavelengths, and calculate the chlorophyll a content using the above formula. There are 3 parallels in the experiment.

[0024] In order to verify the accuracy and reproducibility of this method, the same water sample was taken at the same...

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Abstract

This invention discloses one test method for water errant algae chlorophyll a, which comprises the following steps: getting water errant algae by use of acetate fiber filter film; extracting algae chlorophyll a by use of alcohol; through centrifugation to get upper supernatant layer compared with alcohol without water and using visible light at 630,647,664,750nm to compute water sample chlorophyll content.

Description

technical field [0001] The invention belongs to a method for measuring algae chlorophyll, in particular to a method for measuring algae chlorophyll-a in a water body by using a spectrophotometric method, and is suitable for the determination of various surface waters. Background technique [0002] Chlorophyll is an important photosynthetic pigment in plant photosynthesis. By measuring the chlorophyll of phytoplankton, the situation of primary productivity in the water body can be grasped. In environmental monitoring, the content of chlorophyll a in water can well reflect the biomass of planktonic algae in water, so it is regarded as one of the indicators of eutrophication in water. [0003] There are three methods for the determination of phytoplankton chlorophyll-a in water, namely spectrophotometry, fluorescence and high performance liquid chromatography. Among them, spectrophotometry requires simple instruments, easy operation and low cost, and is the main method curren...

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Application Information

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IPC IPC(8): G01N21/31G01N21/17G01N15/00
Inventor 刘春光孙红文孙凌李贺钟远庄源益
Owner NANKAI UNIV
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