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Purified polyporus laccases and nucleic acids encoding same

一种多孔菌、漆酶的技术，应用在酚氧化酶,纯化酶,漆酶的核酸片段领域，能够解决低产率等问题

Inactive Publication Date: 2007-06-20
NOVO NORDISK AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous attempts to express basidiomycete laccases in recombinant hosts resulted in extremely low yields

Method used

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  • Purified polyporus laccases and nucleic acids encoding same
  • Purified polyporus laccases and nucleic acids encoding same
  • Purified polyporus laccases and nucleic acids encoding same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] I. Isolation of Polyporus pinsitus laccase

[0054] Materials and methods

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Abstract

The present invention relates to isolated nucleic acid constructs containing a sequence encoding a Polyporus laccase, and the laccase proteins encoded thereby.

Description

[0001] This application is a divisional application of the application dated June 15, 1995, application number 03142563.1, and invention titled "Purified Polyporus lacquer and nucleic acid encoding the enzyme". And application 03142563.1 is a divisional application of application 95194301.4. technical field [0002] The present invention relates to isolated nucleic acid fragments encoding a fungal oxidoreductase, and purified enzymes derived from the fragments. More particularly, the present invention relates to nucleic acid fragments encoding a phenoloxidase, in particular a laccase from Basidiomycetes-Polypores. Background technique [0003] Laccases (benzenediol:oxygen oxidoreductases) are multi-copper enzymes that catalyze the oxidation of phenolics. Oxidation of suitable phenolic substrates by laccases leads to the production of aryloxy intermediates that undergo terminal polymerization to form a mixture of dimerization, oligomerization, and polymerization reaction pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/70C12N15/80C12N1/15C12R1/645A61K8/66A61Q5/10D06M16/00D06P1/00D06P1/32D21C5/00
CPCC12N9/0061D06M16/003D06P1/32A61K8/66D21C5/005D06P1/0048D06P1/0004C12N15/80A61Q5/10
Inventor D·S·亚夫尔F·许H·达尔伯吉P·施耐德D·A·阿斯林格
Owner NOVO NORDISK AS