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Application of schisandra fruit in preparation of tyrosine-inhibiting medicine

The technology of tyrosine kinase and Schisandra chinensis is applied in the directions of drug combination, antitumor drug, pharmaceutical formulation, etc., which can solve the problems of drug efficacy and sensitization of tyrosine kinase inhibitory drugs, and achieve the effect of good clinical application prospect.

Inactive Publication Date: 2007-07-11
胡汛 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Schisandra chinensis, the dry mature fruit of Schisandra chihensis (Turcz.) Baill. or Schisandra sphenanthera Rehd.et Wils. of Magnoliaceae, Schisandra sphenanthera Rehd. et Wils. , Yiqi Shengjin, tonifying the kidney and calming the heart, is a nourishing and strong medicine commonly used in traditional Chinese medicine, and has a variety of pharmacological effects, but there is no report on its sensitization to the efficacy of tyrosine kinase inhibitory drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the preparation of Schisandra chinensis ethanol extract

[0015] Percolation method: take 5000g Schisandra chinensis powder and percolate with 6 times the mass of 90% ethanol at a percolation speed of 15ml / min. Recover the ethanol leachate, recover the ethanol under reduced pressure until there is no ethanol smell, concentrate to obtain an extract with a specific gravity of 1.15 g / ml, and obtain the Schisandra chinensis extract for future use. The ethanol used in the above extraction can also be replaced by various alcohols such as methanol and propanol, and the alcohol concentration can be between 75% and 95%; other organic solvents such as ethyl acetate and ether solvents can also be used instead.

Embodiment 2

[0016] Embodiment 2: supercritical extraction

[0017] Supercritical extraction: crush 1000 g of the schisandra sample into a coarse powder (through a 40-mesh sieve), put 1L of supercritical CO 2 In the extraction kettle, adjust the pressure: 25MPa, temperature: 45°C, flow rate: 30kg / h, extraction time: 2h. A brown extract was collected.

Embodiment 3

[0018] Example 3: Schisandra extract enhances the efficacy of Gleevec in killing leukemia cells.

[0019] Experimental method: Schisandra chinensis ethanol extract and supercritical extract were respectively prepared into 20mg / ml mother liquor with DMSO. Human leukemia cell lines Ku812 and K562 (ATCC, USA). K562 or Ku812 are cultured in RPMI-1640 culture medium containing 10% calf serum, inoculated into 24-well cell culture plates, and the cells in each well are 40,000 cells, and then add Example 1 or Example 1 in each well. 2. For the obtained Schisandra extract, add Gleevec to the wells with the same concentration of the Schisandra ethanol extract to the final concentrations of 0, 6, 12, 25, 50, and 100 ng / ml. After culturing for 3 days, count the number of cells in each well with a flow cytometer (FACScan, Becton Dickinson, USA), and then calculate the half inhibitory concentration IC 50 .

[0020] Experimental results:

[0021] Ku812 and K562 are chronic myeloid leukem...

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PUM

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Abstract

The invention relates to a method for using schisandra fruit to prepare the sensitivity-increase drug of tyrosinase restrainer, wherein said restrainer is cancer resistant drug, or the drug that treats abnormal tyrosinase condition. The invention confirms the effect of schisandra fruit to improve the sensitivity of restrainer, with wide application.

Description

(1) Technical field [0001] The invention relates to the application of schisandra chinensis in the preparation of sensitizing drugs for tyrosine kinase inhibitory drugs. (2) Background technology [0002] Currently, tyrosine kinases have been widely accepted as targets for cancer therapy. There are approximately 90 genes for tyrosine kinases in the human genome. In tumors, tyrosine kinases are often abnormal. Gleevec (English name gleevec, or imatinib, or STI571) is an inhibitor against abnormal tyrosine kinases in tumors developed by Novartis. The drug can inhibit abnormal tyrosine kinases in various tumors and is used to treat various tumors, such as leukemia, gastric stromal tumor, glioblastoma, sarcoma, lung cancer and so on. However, the therapeutic effect of Gleevec varies from patient to patient. Some patients are more sensitive to the drug, while others are not. Other tyrosine kinase inhibitors such as gefitinib, erlotinib, Erbitux, etc. also have the same proble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/79A61P35/00A61K131/00
Inventor 胡汛
Owner 胡汛