Methods for producing islet-like cells derived from stem cells, as well as populations and compositions that include them

Abstract

Methods for generating a stem cell-derived islet-like cell (SC-IC) population are described. These methods include culturing precursor cells, such as pancreatic progenitor cells, pancreatic endocrine precursor cells, or immature SC-ICs, using one or more differentiation media that promote the differentiation of the precursor cells into more differentiated cells to obtain an SC-IC population that includes mature pancreatic beta-cell-like cells. Cell differentiation compositions useful in performing the methods and SC-IC populations obtained by performing the methods are also described. Implantable compositions and devices comprising the SC-IC populations, which are useful for the treatment of metabolic disorders such as diabetes mellitus (diabetes), are also described.