Acetyl-coenzyme a carboxylase mutants

Abstract

The present disclosure provides plants and / or plant cells bearing one or more mutations in an acetyl-coenzyme A carboxylase (ACCase) gene, and methods of making and using such plants. In some embodiments, the plants and / or plant cells are resistant or tolerant to an herbicide which targets acetyl-coenzyme A carboxylase.

Description

W O 2 02 4 / 20 69 66 A l (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 03 October 2024 (03.10.2024) W I P O I P C T (10) International Publication Number WO 2024 / 206966 A1 (51) International Patent Classification: C 12N15 / 82 (2006.01) (21) International Application Number: PCT / US2024 / 022430 (22) International Filing Date: 31 March 2024 (31.03.2024) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 63 / 493,442 31 M arch2023 (31.03.2023) US (71) Applicants: CIBUS US LLC [US / US]; 6455 Nancy Ridge Drive, San Diego, California 92121 (US). CIBUS EU­ ROPE B.V. [NL / NL]; Ceresstraat 13,4811 CABreda(NL). (72) Inventors: GOCAL, Gregory F.W.; c / o CIBUS US LLC, 6455 Nancy Ridge Drive, San Diego, California 92121 (US). BEETHAM, Peter R.; c / o CIBUS US LLC, 6455 Nancy Ridge Drive, San Diego, California 92121 (US). WALKER, Keith A.; c / o CIBUS US LLC, 6455 Nan­ cy Ridge Drive, San Diego, California 92121 (US). SAN­ DERS, Steven L.; c / o CIBUS US LLC, 6455 Nancy Ridge Drive, San Diego, California 92121 (US). NAMEIRAK- PAM, Dolendro; c / o CIBUS US LLC, 6455 Nancy Ridge Drive, San Diego, California 92121 (US). (74) Agent: WHITTAKER, Michael A.; ACUITY LAW GROUP, PC, 991 Lomas Santa Fe Drive, Suite C229, Solana Beach, California 92075 (US). (81) Designated States (unless otherwise indicated, for every kind o f national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CV, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IQ, IR, IS, IT, JM, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, MG, MK, MN, MU, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, WS, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind o f regional protection available): ARIPO (BW, CV, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SC, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, ME, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, Cl, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration o f the time limit for amending the claims and to be republished in the event o f receipt o f amendments (Rule 48.2(h)) (54) Title: ACETYL-COENZYME A CARBOXYLASE MUTANTS (57) Abstract: The present disclosure provides plants and / or plant cells bearing one or more mutations in an acetyl-coenzyme A carboxylase (ACCase) gene, and methods of making and using such plants. In some embodiments, the plants and / or plant cells are resistant or tolerant to an herbicide which targets acetyl-coenzyme A carboxylase. WO 2024 / 206966 A1 WO 2024 / 206966 A1 WO 2024 / 206966 PCT / US2024 / 022430 ACETYL-COENZYME A CARBOXYLASE MUTANTS CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present invention claims the benefit of priority to United States Provisional Application Serial No. 63 / 493,442, filed on March 31, 2023, the contents of which are incorporated herein by reference in its entirety. FIELD

[0002] The present disclosure relates to compositions and methods pertaining to novel plant genes and gene products, and to plants having one or more gene mutations. In particular, the present disclosure provides acetyl-coenzyme A carboxylase (ACCase) genes and plants and / or plant cells bearing one or more mutations in an ACCase gene, as well as methods of making and using such plants. In some embodiments, the plant has increased resistance or tolerance to ACCase inhibitor herbicides including clethodim. BACKGROUND

[0003] Acetyl-CoA carboxylase or acetyl-coenzyme A carboxylase (ACCase) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACCase is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum. The most important function of ACCase is to provide the malonyl- CoA substrate for the biosynthesis of fatty acids.

[0004] ACCase inhibitors are a broadly used class of herbicides that preferentially kill grasses. ACCase inhibitors specifically target the plastidal form of the ACCase enzyme present in grass species.

[0005] PCT application WO / 2015 / 139008 (US2015 / 020622), hereby incorporated by reference in its entirety, discloses methods to cause targeted changes to a DNA sequence. PCT application WO 2011 / 028833 (US2013 / 0111618), hereby incorporated by reference in its entirety, discloses herbicide-tolerant plants and methods of producing and treating herbicide-tolerant plants. WO 2024 / 206966 PCT / US2024 / 022430 BRIEF SUMMARY

[0006] The present disclosure is based at least in part on the discovery that certain mutations in the ACCase gene in a plant may result in certain advantages or desirable properties; for example, in some embodiments, may result in resistance to herbicides.

[0007] Accordingly, provided herein include a plant or plant cell having one or more mutations in the ACCase gene and methods of generating such a plant or plant cell. In some embodiments the plant or plant cells are non-transgenic. In certain embodiments the plant has increased resistance or tolerance to an herbicide, for example an herbicide that is a member of the aryloxyphenoxypropanoate, cyclohexanedione, and / or phenylpyrazoline families of herbicides. In various embodiments such plant or plant cell exhibits substantially normal growth or development of the plant, its organs, tissues or cells, in the presence of an herbicide as compared to the corresponding wild-type plant or cell.

[0008] Unless specifically indicated otherwise, the blackgrass (,Alopecurus myosuroides) amino acid numbering depicted in SEQ ID NO: 2 is used herein as the reference for plastidal ACCase. Those of ordinary skill in the art are able to identify analogous corresponding amino acid residues in ACCase genes of ACCase homologs, including orthologs (ACCase from different species) and paralogs (ACCase from the same species). An example of such is shown in the below in which the ACCase protein sequences in Alopecurus myosuroides (Figure IB and SEQ ID NO: 2), Oryza sativa indica (Figure 2B and SEQ ID NO: 4) and Oryza sativa japonica (Figure 3 and SEQ ID NO: 6) are compared, and the listed corresponding amino acid positions identified. Exemplary Amino Acid Positions Am (blackgrass (Alopecurus myosuroides)) Os Indica Os Japonica L1543 L1548 L1548 V1529 V1534 V1534 R1672 R1683 R1670 D1681 D1692 D1679 11781 11792 11779 S1784 S1795 S1782 A1785 A1796 A1783 2 WO 2024 / 206966 PCT / US2024 / 022430 Exemplary Amino Acid Positions L1818 LI 829 L1816 K1840 K1851 K1838 M l 974 M1985 M l 972 A1957 A1968 A1955 V2075 V2086 V2073 D2078 D2089 D2076 P2083 P2094 P2081 C2088 C2099 C2086 N2097 N2108 N2095 V2098 V2109 V2096 E2112 E2123 E2110 12149 12161 12148 A2226 A2238 A2225 R2257 M2264 M2251 N2262 N2269 N2256

[0009] In conjunction with the various aspects, embodiments, compositions, and methods disclosed herein, a plant or plant cell may include a mutated ACCase gene wherein a position of the ACCase gene is changed from the plastidal ACCase amino acid residue to the cytosolic ACCase amino acid residue.

[00010] In one aspect provided is a plant or plant cell having a mutated ACCase gene wherein the gene encodes an ACCase protein including one or more substitutions at one or more amino acid positions corresponding to a position or position combinations selected from the group consisting of the mutations listed on Table 1 and / or methods of making such a plant and or plant cell. In one aspect provided is a plant or plant cell having a mutated ACCase gene wherein the gene encodes an ACCase protein comprising one or more substitutions at one or more amino acid positions corresponding to a position or position combinations selected from the group consisting of the mutations listed on Table 2 and / or methods of making such a plant and or plant cell. In one aspect provided is a plant or plant cell having a mutated ACCase gene wherein the gene encodes an ACCase protein comprising one or more substitutions at one or more amino acid positions corresponding to 3 WO 2024 / 206966 PCT / US2024 / 022430 a position or position combinations selected from the group consisting of the mutations listed on Table 3 and / or methods of making such a plant and or plant cell.

[00011] In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution at one or more amino acid positions corresponding to a position selected from the group consisting of N1460, V I529, L1543, L1555, C1575, S1605, G1605-6A*, N1607, L1649, R1672, D1681, F1683, V1715, D1725, R1734, M1738, T1739, A1752, Q1756, 11766, K1772, N1780, 11781, G1783, S1784, A1785, A1786, S1792, 11811, L1818, Q1824, A1837, K1840, V I864, P1870, S I922, K1930, K1938, E1946, A1957, V1966, 11967, M1974, M1975, V I992, W1999, A2004, W2027, E2039, 12041, V2049, A2059, A2067, W2074, V2075, D2078, S2079, K2080, 12081, P2083, R2085, C2088, K2095, G2096, N2097, V2098, E2112, D2127, 12149, E2150, K2154, Q2155, L2159, E2169, A2178, A2179, V2182, F2196, H2223, A2226, K2232, A2241, S2244, F2253, V2254, R2257, N2262, K2264, 12267, S2276, S2280, S2297, L2299, K2302, and K2317 of SEQ ID NO. 2.

[00012] In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to a substitution selected from the group consisting of N1460S, V1529M, L1543F, L1543V, L1555P, C1575S, S1605Y, G1605-6A*, N1607S, L1649M, R1672I, D1681E, D1681G, F1683L, V1715A, D1725E, R1734G, M1738L, T1739S, A1752T, Q1756P, Q1756E, 11766V, K1772E, K1772N, N1780D, I1781L, I1781T, I1781V, I1781A, G1783C, S1784G, S1784N, A1785G, A1786P, S1792L, 1181 IN, L1818H, L1818I, Q1824P, A1837V, K1840M, K1840R, V1864F, P1870R, S1922G, K1930N, K1938R, E1946G, E1946D, E1946V, A1957T, V1966L, I1967V, M1974T, M1975T, V1992D, W1999C, W1999G, W1999L, W1999S, A2004V, W2027C, W2027R, E2039D, E2039G, 1204ID, 1204IN, I2041V, V2049F, V2049I, V2049L, V2049T, V2049C, V2049A, V2049S, A2059V, A2067S, W2074L, V2075M, V2075G, V2075I, V2075L, D2078G, D2078K, D2078T, D2078V, S2079F, S2079P, K2080E, P2083N, P2083Q, P2083S, P2083T, R2085S, C2088M, C2088N, C2088R, C2088W, C2088F, C2088G, C2088H, C2088K, C2088S, C2088T, C2088L, C2088V, K2095E, K2095N, G2096A, G2096S, N2097D, N2097S, N2097R, N2097K, N2097E, N2097H, N2097A, N2097G, N2097C, N2097P, N2097L, N2097I, N2097M, N2097F, N2097W, N2097Q, N2097T, N2097Y, V2098A, V2098H, V2098P, V2098S, V2098G, V2098C, E2112G, E2112V, D2127N, I2149K, 12149V, E2150K, A2151T, K2154R, Q2155L, L2159P, E2169D, A2178I, A2179T, V2182A, F2196Y, H2223R, A2226S, K2232N, A2241T, F2253I, V2254I, 4 WO 2024 / 206966 PCT / US2024 / 022430 N2262K, K2264R, 12267V, S2276F, S2276P, S2280P, S2297C, L2299I, K2302Q, and K2317T of SEQ ID NO: 2.

[00013] In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to a substitution selected from the group consisting of V1529M, L1543F, L1543V, R1672I, D1681E, D1681G, S1784G, S1784N, A1785G, L1818H, L1818I, K1840M, K1840R, A1957T, M1974T, M1975T, D2078G, P2083T, N2097S, V2098G, E2112V, I2149K, A2226S, and N2262K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to a substitution selected from the group consisting of V1529M, L1543F, L1543V, L1555P, C1575S, S1605Y, G1605-6A*, L1649M, R1672I, D1681E, D1681G, F1683L, V1715A, D1725E, A1752T, I1766V, K1772E, S1784G, S1784N, L1818H, L1818I, K1840M, K1840R, K1930N, A1957T, V1966L, , M1974T, M1975T, V1992D, A2067S, R2085S, E2112V, D2127N, I2149K, E2150K, A2151T, K2154R, Q2155L, L2159P, I2267V, E2169D, A2178I, A2179T, V2182A, F2196Y, H2223R, A2226S, K2232N, F2253I, V2254I, N2262K, S2276F, S2276P, S2280P, S2297C, L2299I, K2302Q, and K2317T of SEQ ID NO: 2.

[00014] In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N1460S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1529M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1543F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to LI 543 V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1555P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C1575S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1605Y of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene 5 WO 2024 / 206966 PCT / US2024 / 022430 encodes an ACCase protein comprising a substitution corresponding to G1605-6A* as described herein. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N1607S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1649M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to R1672I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D1681E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D1681G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to F1683L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1715A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D1725E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to R1734G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to M1738L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to T1739S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1752T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to Q1756P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to Q1756E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene 6 WO 2024 / 206966 PCT / US2024 / 022430 encodes an ACCase protein comprising a substitution corresponding to I1766V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1772E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1772N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N1780D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to G1783C of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1784G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1784N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1785G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1786P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1792L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to 1181 IN of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase 7 WO 2024 / 206966 PCT / US2024 / 022430 protein comprising a substitution corresponding to L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L I8181 of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to Q1824P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1837V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1840M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1840R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1864F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P1870R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1922G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1930N, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1938R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E1946V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E1946G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E1946D, of SEQ ID NO: 2.In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1957T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a 8 WO 2024 / 206966 PCT / US2024 / 022430 substitution corresponding to V1966L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1967V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to M1974T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to M1975T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1992D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999L, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999S, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2004V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W2027C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W2027R, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2039D, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2039G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to 1204 ID, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to 9 WO 2024 / 206966 PCT / US2024 / 022430 1204IN, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I2041V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049C of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2059V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2067S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W2074L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075G of SEQ 10 WO 2024 / 206966 PCT / US2024 / 022430 ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2079F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2079P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2080E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083Q of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to R2085S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088R of SEQ ID NO: 2. In certain 11 WO 2024 / 206966 PCT / US2024 / 022430 embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088W of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2095E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2095N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to G2096A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to G2096S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097S, of SEQ ID NO: 2. In certain embodiments, a plant 12 WO 2024 / 206966 PCT / US2024 / 022430 or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097R, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097K, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097E, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097H, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097A, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097P, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097L, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097I, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097M, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097F, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097W, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097Q, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097T, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a 13 WO 2024 / 206966 PCT / US2024 / 022430 mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097Y, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098H, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098P, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098S, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2112G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2112V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2127N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I2149K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I2149V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2150K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2151T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2154R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase 14 WO 2024 / 206966 PCT / US2024 / 022430 gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to Q2155L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L2159P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2169D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2178I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2179T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2182A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to F2196Y of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to H2223R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2226S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2232N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2241T, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to F2253I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2254I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2262K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2264R, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene 15 WO 2024 / 206966 PCT / US2024 / 022430 encodes an ACCase protein comprising a substitution corresponding to I2267V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2276F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2276P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2280P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2297C of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L2299I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2302Q of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2317T of SEQ ID NO: 2.

[00015] In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N1460S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a mutation or mutation combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1529M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1543F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to LI543V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 16 WO 2024 / 206966 PCT / US2024 / 022430 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1555P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C1575S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1605Y of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to G1605-6A* of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N1607S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1649M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to R1672I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D1681E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a 17 WO 2024 / 206966 PCT / US2024 / 022430 substitution corresponding to D1681G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to F1683L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1715A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D1725E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to R1734G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to M1738L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to T1739S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1752T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a 18 WO 2024 / 206966 PCT / US2024 / 022430 substitution corresponding to Q1756P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to Q1756E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to II766V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1772E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1772N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N1780D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 19 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I1781A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to G1783C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1784G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1784N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1785G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1786P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1792L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to 1181 IN of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 20 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1818H of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L1818I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to Q1824P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1837V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1840M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1840R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1864F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P1870R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 21 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S1922G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1930N, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K1938R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E1946G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E1946D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E1946V, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A1957T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1966L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 22 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to II967V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to M1974T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to M1975T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V1992D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W1999S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 23 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2004V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W2027C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to W2027R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2039D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2039G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I2041D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I2041N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I2041V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 24 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2049S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2059V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 25 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2067S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2074L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2 or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2075L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 26 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2079F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2079P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2080E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083Q of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 27 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to R2085S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088W of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 28 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088H of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to C2088V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2095E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2095N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 29 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to G2096A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to G2096S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097S, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097R, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097K, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097E, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097H, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 30 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097A, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097G, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097C, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097P, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097L, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097I, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097M, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097F, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 31 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097W, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097Q, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097T, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2097Y, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098G, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098H, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098P, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 32 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098S, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2098C, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2112G, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2112V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2127N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to I2149K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2150K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2151T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 33 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2154R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to Q2155L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L2159P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to E2169D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2178I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2179T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2182A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to F2196Y of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a 34 WO 2024 / 206966 PCT / US2024 / 022430 substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to H2223R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2226S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2232N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to A2241T, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to F2253I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to V2254I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to N2262K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2264R, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the 35 WO 2024 / 206966 PCT / US2024 / 022430 gene encodes an ACCase protein comprising a substitution corresponding to I2267V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2276F, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2276P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2280P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to S2297C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to L2299I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2302Q of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to K2317T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). 36 WO 2024 / 206966 PCT / US2024 / 022430

[00016] In certain embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2 (i.e ., wherein the encoded protein has any amino acid other than an aspartate at the position corresponding to D2078 of SEQ ID NO: 2). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to D2078V of SEQ ID NO: 2. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided herein. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 1. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 2. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation as provided in Table 3. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2. 37 WO 2024 / 206966 PCT / US2024 / 022430

[00017] In certain embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (i.e ., wherein the encoded protein has any amino acid other than a proline at the position corresponding to P2083 of SEQ ID NO: 2). In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083Q of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein comprising a substitution corresponding to P2083T of SEQ ID NO: 2. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided herein. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 1. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 2. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation as provided in Table 3. In some embodiments, a plant or plant cell provided herein may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2. 38 WO 2024 / 206966 PCT / US2024 / 022430

[00018] In some embodiments, a plant or plant cell provided herein may have two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2078 and P2083 of SEQ ID NO: 2. In some embodiments, the substitution at the amino acid position corresponding to position D2078 of SEQ ID NO: 2 is selected from the group including, but not limited to: D2078G, D2078K, D2078T, and D2078V. In some embodiments, the substitution at the amino acid position corresponding to position D2078 of SEQ ID NO: 2 is D2078G. In some embodiments, the substitution at the amino acid position corresponding to position P2083 of SEQ ID NO: 2 is selected from the group including, but not limited to: P2083T, P2083S, P2083N, and P2083Q. In some embodiments, the substitution at the amino acid position corresponding to position P2083 of SEQ ID NO: 2 is P2083T. In some embodiments, a plant or plant cell provided herein may have two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2078G and P2083T of SEQ ID NO: 2.

[00019] In some embodiments, a plant or plant cell provided herein may have two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2089 and P2094 of SEQ ID NO: 4 or SEQ ID NO: 954. In some embodiments, the substitution at the amino acid position corresponding to position D2089 of SEQ ID NO: 4 or SEQ ID NO: 954 is selected from the group including, but not limited to: D2089G, D2089K, D2089T, and D2089V. In some embodiments, the substitution at the amino acid position corresponding to position D2089 of SEQ ID NO: 4 or SEQ ID NO: 954 is D2089G. In some embodiments, the substitution at the amino acid position corresponding to position P2094 of SEQ ID NO: 4 or SEQ ID NO: 954 is selected from the group including, but not limited to: P2094T, P2094S, P2094N, and P2094Q. In some embodiments, the substitution at the amino acid position corresponding to position P2094 of SEQ ID NO: 4 or SEQ ID NO: 954 is P2094T. In some embodiments, a plant or plant cell provided herein may have two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2089G and P2094T of SEQ ID NO: 4 or SEQ ID NO: 954.

[00020] In some embodiments, a plant or plant cell provided herein may have two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising at least two amino acid substitutions at the amino acid positions corresponding 39 WO 2024 / 206966 PCT / US2024 / 022430 to D2076 and P2081 of SEQ ID NO: 6. In some embodiments, the substitution at the amino acid position corresponding to position D2076 of SEQ ID NO: 6 is selected from the group including, but not limited to: D2076G, D2076K, D2076T, and D2076V. In some embodiments, the substitution at the amino acid position corresponding to position D2076 of SEQ ID NO: 2 is D2076G. In some embodiments, the substitution at the amino acid position corresponding to position P2081 of SEQ ID NO: 6 is selected from the group including, but not limited to: P2081T, P2081S, P2081N, and P2081Q. In some embodiments, the substitution at the amino acid position corresponding to position P2081 of SEQ ID NO: 6 is P2081T. In some embodiments, a plant or plant cell provided herein may have two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2076G and P2081T of SEQ ID NO: 6.

[00021] The present disclosure also relates to a method for producing a plant having a mutated ACCase gene that substantially maintains the catalytic activity of the wild-type protein regardless of the presence or absence of an herbicide of the aryloxyphenoxypropanoate, cyclohexanedione, and / or phenylpyrazoline families. The method may include introducing into a plant cell an ACCase gene that produces a gene product having one or more of the amino acid changes described herein or by mutating or engineering an ACCase gene such that the gene produces a gene product having one or more of the amino acid changes described herein. The method may include introducing into a plant cell an oligonucleobase with a targeted mutation(s) in the ACCase gene that produces a gene product having one or more of the amino acid changes described herein.

[00022] The method further includes identifying a cell, seed, or plant having a mutated ACCase gene and to culturing and regeneration methods to obtain a plant that produces seeds, henceforth a “fertile plant”, and the production of seeds and additional plants from such a fertile plant including descendant (progeny) plants that contain the mutated ACCase gene. By way of example, a D2078G mutation (numbering based on SEQ ID NO: 2) can exhibit normal plant growth and strong herbicide tolerance but sterility due to poor pollen viability and inability to produce seeds by self-pollination. A D2078G / P2083T (numbering based on SEQ ID NO: 2) double mutation can exhibit the normal growth rate and herbicide tolerance, but regain fertility.

[00023] The disclosure in various embodiments is also directed to novel mutations in the ACCase gene and resulting novel gene product that confer resistance or tolerance to a member of the aryloxyphenoxypropanoate, cyclohexanedione, and / or phenylpyrazoline 40 WO 2024 / 206966 PCT / US2024 / 022430 families, e.g ., one or more of alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, profoxydim, sethoxydim, tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, diclofop-methyl, fenoxaprop, fenoxaprop-P-ethyl, fenthiaprop, fluazifop, fluazifop-P- butyl, haloxyfop, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P-ethyl, trifop, pinoxaden, agronomically acceptable salts and esters of any of these herbicides, and combinations thereof, to an plant or wherein the mutated ACCase has substantially the same enzymatic activity as compared to wild-type ACCase.

[00024] In conjunction with the various aspects, embodiments, compositions, and methods disclosed herein, a plant or plant cell may express a mutated ACCase protein wherein one or more residues of the ACCase gene are changed from the plastidal amino acid residue to the cytosolic amino acid residue.

[00025] In a related aspect provided is a plant or plant cell expressing a mutated ACCase protein comprising one or more substitutions at one or more amino acid positions corresponding to a position or position combinations selected from the group consisting of the mutations listed on Table 1 and / or methods of making such a plant and or plant cell. In one embodiment, the ACCase protein comprises a substitution at one or more amino acid positions corresponding to a position or position combinations selected from the group consisting of the substitutions listed on Table 2. In one aspect provided is a plant or plant cell expressing a mutated ACCase protein comprising a substitution at one or more amino acid positions corresponding to a position or position combinations selected from the group consisting of the substitutions listed on Table 3 and / or methods of making such a plant and or plant cell.

[00026] In certain embodiments, a plant or plant cell may express a protein comprising one or more substitutions at one or more amino acid positions corresponding to a position selected from the group consisting of N1460, V1529, L1543, L1555, C1575, S1605, G1605-6A*, N1607, L1649, R1672, D1681, F1683, V1715, D1725, R1734, M1738, T1739, A1752, Q1756, 11766, K1772, N1780, 11781, G1783, S1784, A1785, A1786, S1792, 11811, L1818, Q1824, A1837, K1840, V1864, P1870, S1922, K1930, K1938, E1946, A1957, V1966, 11967, M1974, M1975, V1992, W1999, A2004, W2027, E2039, 12041, V2049, A2059, A2067, W2074, V2075, D2078, S2079, K2080, 12081, P2083, R2085, C2088, K2095, G2096, N2097, V2098, E2112, D2127, 12149, E2150, K2154, Q2155, L2159, E2169, A2178, A2179, V2182, F2196, H2223, A2226, K2232, A2241, S2244, F2253, V2254, R2257, N2262, K2264, 12267, S2276, S2280, S2297, L2299, K2302, and K2317 of SEQ ID NO: 2. 41 WO 2024 / 206966 PCT / US2024 / 022430

[00027] In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising a substitution at an amino acid position corresponding to a position selected from the group consisting of N1460S, V1529M, L1543F, L1543V, L1555P, C1575S, S1605Y, G1605-6A*, N1607S, L1649M, R1672I, D1681E, D1681G, F1683L, V1715A, D1725E, R1734G, M1738L, T1739S, A1752T, Q1756P, Q1756E, I1766V, K1772E, K1772N, N1780D, I1781L, I1781T, I1781V, I1781A, G1783C, S1784G, S1784N, A1785G, A1786P, S1792L, 1181 IN, L1818H, L1818I, Q1824P, A1837V, K1840M, K1840R, V1864F, P1870R, S1922G, K1930N, K1938R, E1946G, E1946D, El946V, A1957T, V1966L, I1967V, M1974T, M1975T, V1992D, W1999C, W1999G, W1999L, W1999S, A2004V, W2027C, W2027R, E2039D, E2039G, I2041D, I2041N, 1204IV, V2049F, V2049I, V2049L, V2049T, V2049C, V2049A, V2049S, A2059V, A2067S, W2074L, V2075M, V2075G, V2075I, V2075L, D2078G, D2078K, D2078T, D2078V, S2079F, S2079P, K2080E, P2083N, P2083Q, P2083S, P2083T, R2085S, C2088M, C2088N, C2088R, C2088W, C2088F, C2088G, C2088H, C2088K, C2088S, C2088T, C2088L, C2088V, K2095E, K2095N, G2096A, G2096S, N2097D, N2097S, N2097R, N2097K, N2097E, N2097H, N2097A, N2097G, N2097C, N2097P, N2097L, N2097I, N2097M, N2097F, N2097W, N2097Q, N2097T, N2097Y, V2098A, V2098H, V2098P, V2098S, V2098G, V2098C, E2112G, E2112V, D2127N, I2149K, I2149V, E2150K, A2151T, K2154R, Q2155L, L2159P, E2169D, A2178I, A2179T, V2182A, F2196Y, H2223R, A2226S, K2232N, A2241T, F2253I, V2254I, N2262K, K2264R, 12267V, S2276F, S2276P, S2280P, S2297C, L2299I, K2302Q, and K2317T of SEQ ID NO: 2.

[00028] In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising one or more substitutions corresponding to one or more substitutions selected from the group consisting of V1529M, L1543F, LI543V, R1672I, D 168IE, D1681G, S1784G, S1784N, A1785G, L1818H, L1818I, K1840M, K1840R, A1957T, M1974T, M1975T, D2078G, P2083T, N2097S, V2098G, E2112V, I2149K, A2226S, and N2262K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising one or more substitutions corresponding to one or more substitutions selected from the group consisting ofV1529M, L1543F, LI543V, L1555P, C1575S, S1605Y, G1605-6A*, L1649M, R1672I, D1681E, D1681G, F1683L, V1715A, D1725E, A1752T, I1766V, K1772E, S1784G, S1784N, L1818H, L1818I, K1840M, K1840R, K1930N, A1957T, V1966L, , M1974T, M1975T, V1992D, A2067S, R2085S, E2112V, D2127N, I2149K, E2150K, A2151T, K2154R, Q2155L, L2159P, 42 WO 2024 / 206966 PCT / US2024 / 022430 I2267V, E2169D, A2178I, A2179T, V2182A, F2196Y, H2223R, A2226S, K2232N, F2253I, V2254I, N2262K, S2276F, S2276P, S2280P, S2297C, L2299I, K2302Q, and K2317T of SEQ ID NO: 2.

[00029] In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N1460S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1529M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1543F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1543V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1555P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C1575S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1605Y of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G1605-6A* as described herein. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N1607S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1649M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to R1672I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D1681E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D1681G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to F1683L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1715A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 43 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to D1725E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to R1734G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to M1738L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to T1739S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1752T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q1756P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q1756E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1766V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1772E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1772N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N1780D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G1783C of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1784G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 44 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to S1784N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1785G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1786P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1792L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to 1181 IN of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1818I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q1824P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1837V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1840M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1840R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1864F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P1870R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1922G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1930N, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1938R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E1946V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 45 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to E1946G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E1946D, of SEQ ID NO: 2.In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1957T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1966L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1967V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to M1974T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to M1975T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1992D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999L, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999S, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2004V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W2027C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W2027R, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2039D, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2039G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 46 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to I2041D, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to 1204IN, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2041V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049C of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2059V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2067S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W2074L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 47 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2078K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2078T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2078V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2079F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2079P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2080E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083Q of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to R2085S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088W of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 48 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to C2088G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2095E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2095N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G2096A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G2096S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097S, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097R, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097K, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097E, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097H, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 49 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to N2097A, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097P, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097L, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097I, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097M, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097F, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097W, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097Q, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097T, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097Y, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098H, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098P, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098S, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 50 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to V2098C, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2112G, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2112V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2127N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2149K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2149V, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2150K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2151T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2154R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q2155L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L2159P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2169D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2178I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2179T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2182A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to F2196Y of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to H2223R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 51 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to A2226S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2232N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2241T, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to F2253I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2254I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2262K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2264R, of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2267V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2276F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2276P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2280P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2297C of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L2299I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2302Q of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2317T of SEQ ID NO: 2.

[00030] In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N1460S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein 52 WO 2024 / 206966 PCT / US2024 / 022430 comprising an amino acid substitution corresponding to V1529M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1543F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to LI543V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1555P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C1575S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1605Y of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G1605-6A* of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N1607S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1649M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 53 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to R1672I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D1681E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D1681G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to F1683L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1715A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D1725E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to R1734G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to M1738L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to T1739S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 54 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1752T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q1756P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q1756E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1766V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1772E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1772N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N1780D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 55 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I1781A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G1783C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1784G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1784N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1785G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1786P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1792L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 56 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to 1181 IN of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L1818H of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L I8181 of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q1824P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1837V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1840M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1840R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1864F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P1870R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 57 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S1922G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1930N, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K1938R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E1946G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E1946D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E1946V, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A1957T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1966L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 58 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to I1967V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to M1974T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to M1975T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V1992D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W1999S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2004V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 59 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W2027C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to W2027R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2039D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2039G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2041D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2041N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2041V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 60 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to V2049I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2049S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2059V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2067S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2074L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 61 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2 or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2075L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2078G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2078K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2078T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2078V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 62 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to S2079F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2079P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2080E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083Q of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to P2083T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to R2085S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088M of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 63 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088W of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088F of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088G of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088H of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 64 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to C2088T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to C2088V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2095E of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2095N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G2096A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to G2096S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097S, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 65 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097R, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097K, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097E, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097H, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097A, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097G, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097C, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097P, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 66 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to N2097L, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097I, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097M, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097F, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097W, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097Q, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097T, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2097Y, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 67 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098G, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098H, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098P, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098S, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2098C, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2112G, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2112V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to D2127N of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 68 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to I2149K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2150K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2151T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2154R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to Q2155L of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L2159P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to E2169D of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2178I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2179T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 69 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2182A of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to F2196Y of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to H2223R of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2226S of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2232N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to A2241T, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to F2253I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to V2254I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to N2262K of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution 70 WO 2024 / 206966 PCT / US2024 / 022430 or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2264R, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to I2267V of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2276F, of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2276P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2280P of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to S2297C of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to L2299I of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution corresponding to K2302Q of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an 71 WO 2024 / 206966 PCT / US2024 / 022430 amino acid substitution corresponding to K2317T of SEQ ID NO: 2 and an additional substitution or substitution combination such as provided herein (for example a substitution or substitution combination provided in Table 1, Table 2, or Table 3).

[00031] In certain embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078 of SEQ ID NO: 2 (i.e ., wherein the encoded protein has any amino acid other than an aspartate at the position corresponding to D2078 of SEQ ID NO: 2). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078V of SEQ ID NO: 2. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided herein. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 1. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 2. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation as provided in Table 3. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to D2078 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation at an amino acid position corresponding to P2083 of SEQ ID NO: 2. 72 WO 2024 / 206966 PCT / US2024 / 022430

[00032] In certain embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising a substitution at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (i.e ., wherein the encoded protein has any amino acid other than a proline at the position corresponding to P2083 of SEQ ID NO: 2). In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to P2083N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to P2083Q of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to P2083S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell expresses a mutated ACCase protein comprising an amino acid substitution at a position corresponding to P2083T of SEQ ID NO: 2. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising a substitution at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided herein. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising a substitution at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 1. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising a substitution at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation or mutations as provided in Table 2. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising a substitution at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation as provided in Table 3. In some embodiments, a plant or plant cell provided herein expresses a mutated ACCase protein comprising a substitution at an amino acid position corresponding to P2083 of SEQ ID NO: 2 (such as a substitution as provided herein) in combination with an additional ACCase mutation at an amino acid position corresponding to D2078 of SEQ ID NO: 2.

[00033] In some embodiments, a plant or plant cell provided herein expresses a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2078 and P2083 of SEQ ID NO: 2. In some embodiments, the substitution at the amino 73 WO 2024 / 206966 PCT / US2024 / 022430 acid position corresponding to position D2078 of SEQ ID NO: 2 is selected from the group including, but not limited to: D2078G, D2078K, D2078T, and D2078V. In some embodiments, the substitution at the amino acid position corresponding to position D2078 of SEQ ID NO: 2 is D2078G. In some embodiments, the substitution at the amino acid position corresponding to position P2083 of SEQ ID NO: 2 is selected from the group including, but not limited to: P2083T, P2083S, P2083N, and P2083Q. In some embodiments, the substitution at the amino acid position corresponding to position P2083 of SEQ ID NO: 2 is P2083T. In some embodiments, a plant or plant cell provided herein expresses a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2078G and P2083T of SEQ ID NO: 2.

[00034] In some embodiments, a plant or plant cell provided herein expresses a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2089 and P2094 of SEQ ID NO: 4 or SEQ ID NO: 954. In some embodiments, the substitution at the amino acid position corresponding to position D2089 of SEQ ID NO: 4 or SEQ ID NO: 954 is selected from the group including, but not limited to: D2089G, D2089K, D2089T, and D2089V. In some embodiments, the substitution at the amino acid position corresponding to position D2089 of SEQ ID NO: 4 or SEQ ID NO: 954 is D2089G. In some embodiments, the substitution at the amino acid position corresponding to position P2094 of SEQ ID NO: 4 or SEQ ID NO: 954 is selected from the group including, but not limited to: P2094T, P2094S, P2094N, and P2094Q. In some embodiments, the substitution at the amino acid position corresponding to position P2094 of SEQ ID NO: 4 or SEQ ID NO: 954 is P2094T. In some embodiments, a plant or plant cell provided herein expresses a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2089G and P2094T of SEQ ID NO: 4 or SEQ ID NO: 954.

[00035] In some embodiments, a plant or plant cell provided herein expresses a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2076 and P2081 of SEQ ID NO: 6. In some embodiments, the substitution at the amino acid position corresponding to position D2076 of SEQ ID NO: 6 is selected from the group including, but not limited to: D2076G, D2076K, D2076T, and D2076V. In some embodiments, the substitution at the amino acid position corresponding to position D2076 of SEQ ID NO: 2 is D2076G. In some embodiments, the substitution at the amino acid position corresponding to position P2081 of SEQ ID NO: 6 is selected from the group including, but not limited to: P2081T, P2081S, P2081N, and P2081Q. In some embodiments, the substitution at the amino acid position corresponding to position P2081 74 WO 2024 / 206966 PCT / US2024 / 022430 of SEQ ID NO: 6 is P2081T. In some embodiments, a plant or plant cell provided herein expresses a protein comprising at least two amino acid substitutions at the amino acid positions corresponding to D2076G and P2081T of SEQ ID NO: 6.

[00036] The present disclosure also relates to a method for producing a plant having a mutated ACCase gene that substantially maintains the catalytic activity of the wild-type protein regardless of the presence or absence of an herbicide of the aryloxyphenoxypropanoate, cyclohexanedione, and / or phenylpyrazoline families. The method may include introducing into a plant cell an ACCase gene that produces a gene product having one or more of the amino acid changes described herein or by mutating or engineering an ACCase gene such that the gene produces a gene product having one or more of the amino acid changes described herein. The method may include introducing into a plant cell an oligonucleobase with a targeted mutation(s) in the ACCase gene that produces a gene product having one or more of the amino acid changes described herein. The method further includes identifying a cell, seed, or plant having a mutated ACCase gene and to culturing and regeneration methods to obtain a plant that produces seeds, henceforth a “fertile plant”, and the production of seeds and additional plants from such a fertile plant including descendant (progeny) plants that contain the mutated ACCase gene.

[00037] The present disclosure also relates to modified ACCase proteins as described herein, nucleic acides encoding such proteins, and methods of expressing such proteins.

[00038] In certain embodiments, a mutated plant as provided herein is resistant to one or more of alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, profoxydim, sethoxydim, tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, diclofop-methyl, fenoxaprop, fenoxaprop-P-ethyl, fenthiaprop, fluazifop, fluazifop-P-butyl, haloxyfop, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P-ethyl, trifop, pinoxaden, agronomically acceptable salts and esters of any of these herbicides, and combinations thereof, and in certain aspects retains substantially the same catalytic activity as compared to the wild-type corresponding ACCase protein.

[00039] The disclosure is further directed to a method of selectively controlling weeds in a field. The field may include plants with the disclosed ACCase gene alterations. The method comprises application to the field of an herbicide of the aryloxyphenoxypropanoate, cyclohexanedione, and / or phenylpyrazoline families to which the plants are rendered resistant, and the weeds are controlled. A preferred herbicide may be one or more of alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, profoxydim, sethoxydim, tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, 75 WO 2024 / 206966 PCT / US2024 / 022430 diclofop-methyl, fenoxaprop, fenoxaprop-P-ethyl, fenthiaprop, fluazifop, fluazifop-P- butyl, haloxyfop, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P-ethyl, trifop, pinoxaden, agronomically acceptable salts and esters of any of these herbicides, and combinations thereof. BRIEF DESCRIPTION OF THE FIGURES

[00040] Fig. 1A depicts a cDNA sequence of A lo p ecu ru s m yo su ro id es plastidal ACCase AMY310767 (SEQ ID NO: 1).

[00041] Fig. IB depicts an amino acid sequence of A lo p ecu ru s m yo su ro id es plastidal ACCase AMY310767 (SEQ ID NO: 2).

[00042] Fig. 2A depicts a cDNA sequence of O ryza sa tiv a (Cyprus) plastidal ACCase Os05g22940 (SEQ ID NO: 3).

[00043] Fig. 2B depicts an amino acid sequence of O ryza sa tiv a (Cyprus) plastidal ACCase Os05g22940 (SEQ ID NO: 4).

[00044] Fig. 2C depicts a genomic DNA - reverse complemented fragment sequence of O ryza sa tiva (Cyprus) plastidal ACCase Os05g22940 (SEQ ID NO: 5).

[00045] Fig. 3 depicts an amino acid sequence of O ryza sa tiva (Japonica) plastidal ACCase Os05g22940 (SEQ ID NO: 6).

[00046] Fig. 4A depicts a cDNA sequence of T riticum a estivu m plastidal ACCase AF29895 (SEQ ID NO: 7).

[00047] Fig. 4B depicts an amino acid sequence of T riticum a estivu m plastidal ACCase AF29895 (SEQ ID NO: 8).

[00048] Fig. 5A depicts a cDNA sequence of Z ea m a ys plastidal ACCase la AY312171 (SEQ ID NO: 9).

[00049] Fig. 5B depicts an amino acid sequence of Z ea m a ys plastidal ACCase la AY312171 (SEQ ID NO: 10).

[00050] Fig. 6A depicts a cDNA sequence of Z ea m a ys plastidal ACCase lb AY312172 (SEQ ID NO: 11).

[00051] Fig. 6B depicts an amino acid sequence of Z ea m a ys plastidal ACCase lb AY312172 (SEQ ID NO: 12.

[00052] Fig. 7A depicts a cDNA sequence of Z ea m a ys plastidal ACCase ZMU19183 (SEQ ID NO: 13). 76 WO 2024 / 206966 PCT / US2024 / 022430

[00053] Fig. 7B depicts an amino acid sequence of Z ea m a ys plastidal ACCase ZMU19183 (SEQ ID NO: 14).

[00054] Fig. 8A depicts a cDNA sequence of S e ta r ia itá lica plastidal ACCase AY219174 (SEQ ID NO: 15).

[00055] Fig. 8B depicts an amino acid sequence of S e ta ria itá lica plastidal ACCase AY219174 (SEQ ID NO: 16).

[00056] Fig. 9A depicts a cDNA sequence of S e ta r ia itá lica plastidal ACCase AY219175 DIM tolerant (SEQ ID NO: 17).

[00057] Fig. 9B depicts an amino acid sequence of S e ta ria itá lica plastidal ACCase AY219175 DIM tolerant (SEQ ID NO: 18).

[00058] Fig. 10A depicts a cDNA sequence of S e ta ria itá lica plastidal ACCase AF294805 (SEQ ID NO: 19).

[00059] Fig. 10B depicts an amino acid sequence of S e ta ria itá lica plastidal ACCase AF294805 (SEQ ID NO: 20).

[00060] Fig. 11A depicts a cDNA sequence of So rg h u m b ico lo r plastidal ACCase Sb06003090 (SEQ ID NO: 21).

[00061] Fig. 11B depicts an amino acid sequence of S o rg h u m b ico lo r plastidal ACCase Sb06003090 (SEQ ID NO: 22).

[00062] Fig. 12 depicts an amino acid sequence of B ra ch yp o d iu m d is ta ch yo n plastidal ACCase Bradi5g03860 (SEQ ID NO: 23).

[00063] Fig. 13A depicts a cDNA sequence of Z ea m a ys plastidal ACCase NC050105.1 (SEQ ID NO: 950)

[00064] Fig. 13B depicts an amino acid sequence of Z ea m a ys plastidal ACCase NC050105.1 (SEQ ID NO: 951)

[00065] Fig. 14A depicts a cDNA sequence of So rg h u m b ico lo r plastidal ACCase NC012875 (SEQ ID NO: 952)

[00066] Fig. 14B depicts an amino acid sequence of S o rg h u m b ico lo r plastidal ACCase NC012875 (SEQ ID NO: 953)

[00067] Fig. 15 depicts an amino acid sequence of O ryza sa tiva subsp. Japónica plastidal ACCase NP001407229.1 (SEQ ID NO: 954)

[00068] Fig. 16A depicts a cDNA sequence of H o rd eu m vu lg a re plastidal ACCase NC058519.1 (SEQ ID NO: 955)

[00069] Fig. 16B depicts an amino acid sequence of H o rd eu m vu lg a re plastidal ACCase NC058519.1 (SEQ ID NO: 956) 77 WO 2024 / 206966 PCT / US2024 / 022430

[00070] Fig. 17A depicts a cDNA sequence of T riticum a estivu m plastidal ACCase NC057799.1 (SEQ ID NO: 957)

[00071] Fig. 17B depicts an amino acid sequence of Triticum a estivu m plastidal ACCase NC057799.1 (SEQ ID NO: 958)

[00072] Fig. 18A depicts a cDNA sequence of T riticum a estivu m plastidal ACCase NC057798.1 (SEQ ID NO: 959)

[00073] Fig. 18B depicts an amino acid sequence of Triticum a estivu m plastidal ACCase NC057798.1 (SEQ ID NO: 960)

[00074] Fig. 19A depicts a cDNA sequence of T riticum a estivu m plastidal ACCase NC057797.1 (SEQ ID NO: 961)

[00075] Fig. 19B depicts an amino acid sequence of Triticum a estivu m plastidal ACCase NC057797.1 (SEQ ID NO: 962)

[00076] Fig. 20A depicts a cDNA sequence of S e ta ria itá lica plastidal ACCase NC028456.1 (SEQ ID NO: 963)

[00077] Fig. 20B depicts an amino acid sequence of S e ta ria itá lica plastidal ACCase NC028456.1 (SEQ ID NO: 964)

[00078] Fig. 21A depicts a cDNA sequence of L o liu m p e re n n e plastidal ACCase NC067245.1 (SEQ ID NO: 965)

[00079] Fig. 21B depicts an amino acid sequence of L o liu m p e re n n e plastidal ACCase NC067245.1 (SEQ ID NO: 966)

[00080] Fig. 22A depicts a cDNA sequence of P a n icu m virg a tu m plastidal ACCase NC053142.1 (SEQ ID NO: 967)

[00081] Fig. 22B depicts an amino acid sequence of P a n icu m virg a tu m plastidal ACCase NC053142.1 (SEQ ID NO: 968)

[00082] Fig. 23A depicts a cDNA sequence of A v e n a sa tiv a plastidal ACCase PepsiCoV2 chr4C (SEQ ID NO: 969)

[00083] Fig. 23B depicts an amino acid sequence of A v e n a sa tiva plastidal ACCase PepsiCoV2 chr4C (SEQ ID NO: 970)

[00084] Fig. 24A depicts a cDNA sequence of A v e n a sa tiv a plastidal ACCase PepsiCoV2 chr5D (SEQ ID NO: 971)

[00085] Fig. 24B depicts an amino acid sequence of A v e n a sa tiva plastidal ACCase PepsiCoV2 chr5D (SEQ ID NO: 972)

[00086] Fig. 25A depicts a cDNA sequence of A v e n a sa tiv a plastidal ACCase PepsiCoV2 chr6A (SEQ ID NO: 973) 78 WO 2024 / 206966 PCT / US2024 / 022430

[00087] Fig. 25B depicts an amino acid sequence of A v e n a sa tiva plastidal ACCase PepsiCoV2 chr6A (SEQ ID NO: 974)

[00088] Fig. 26A depicts a cDNA sequence of P o a p ra te n s is plastidal ACCase CAMZMU010000063 (SEQ ID NO: 975)

[00089] Fig. 26B depicts an amino acid sequence of P o a p ra te n s is plastidal ACCase CAMZMU010000063 (SEQ ID NO: 976)

[00090] Fig. 27A depicts a cDNA sequence of P o a p ra te n s is plastidal ACCase CAMZMU010000098 (SEQ ID NO: 977)

[00091] Fig. 27B depicts an amino acid sequence of P o a p ra te n s is plastidal ACCase CAMZMUO10000098 (SEQ ID NO: 978) DETAILED DESCRIPTION

[00092] In various aspects and embodiments of the present disclosure, provided include a plant or plant cell having one or more ACCase mutations and / or mutation combinations, methods of making such a plant or plant cell, and methods for producing plants having desirable clethodim tolerance. In various aspects and embodiments of the present disclosure, provided include a plant or plant cell having one or more ACCase mutations and / or mutation combinations, methods of making such a plant or plant cell, and methods for producing plants having desirable fertility.

[00093] It will be readily apparent to a person skilled in the art that varying substitutions and modifications may be made to this disclosure without departing from the scope and spirit of the disclosure.

[00094] The disclosure illustratively described herein suitably may be practiced in the absence of any element or elements, limitation or limitations which is not specifically disclosed herein. Thus, for example, in each instance herein any of the terms “comprising”, “consisting essentially o f ’ and “consisting o f ’ may be replaced with either of the other two terms. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention that in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the disclosure claimed. Thus, it should be understood that although the present disclosure has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, 79 WO 2024 / 206966 PCT / US2024 / 022430 and that such modifications and variations are considered to be within the scope of this disclosure as defined by the appended claims.

[00095] Thus, it should be understood that although the present disclosure has been specifically disclosed by preferred embodiments and optional features, modification, improvement, and variation of the disclosures disclosed may be resorted to by those skilled in the art, and that such modifications, improvements and variations are considered to be within the scope of this disclosure. The materials, methods, and examples provided here are representative of preferred embodiments, are exemplary, and are not intended as limitations on the scope of the disclosure.

[00096] The disclosure has been described broadly and generically herein. Each of the narrower species and subgeneric groupings falling within the generic disclosure also form part of the disclosure. This includes the generic description of the disclosure with a proviso or negative limitation removing any subject matter from the genus, regardless of whether the excised material is specifically recited herein.

[00097] In addition, where features or aspects of the disclosure are described in terms of Markush groups, those skilled in the art will recognize that the disclosure is also thereby described in terms of any individual member or subgroup of members of the Markush group.

[00098] All publications, patent applications, patents, and other references mentioned herein are expressly incorporated by reference in their entirety, to the same extent as if each were incorporated by reference individually. In case of conflict, the present specification, including definitions, will control. Acetyl-CoA Carboxylase (ACCase) genes

[00099] The present disclosure generally relates to plants having mutations in acetyl- CoA carboxylase (ACCase) genes. In some embodiments, one or more mutations in an ACCase gene results in increased herbicide resistance or tolerance. In some embodiments, one or more mutations in an ACCase gene results in increased clethodim resistance or tolerance. A set of exemplary nucleic acid and corresponding amino acid sequences are provided in the Figures. [000100] The A lo p ecu ru s m yo su ro id es plastidal ACCase O ryza sa tiva ACCase gene product has the following sequence (SEQ ID NO: 2; Figure IB) 1 MGSTHLPIVG FNASTTPSLS TLRQINSAAA AFQSSSPSRS SKKKSRRVKS 80 WO 2024 / 206966 PCT / US2024 / 022430 51 IRDDGDGSVP DPAGHGQSIR QGLAGIIDLP KEGASAPDVD ISHGSEDHKA 101 SYQMNGILNE SHNGRHASLS KVYEFCTELG GKTPIHSVLV ANNGMAAAKF 151 MRSVRTWAND TFGSEKAIQL IÄMATPEDMR INAEHIRIAD QFVEVPGGTN 201 NNNYANVQLI VEIAERTGVS AVWPGWGHAS ENPELPDALT AKGIVFLGPP 251 ASSMNALGDK VGSALIAQAA GVPTLAWSGS HVEIPLELCL DSIPEEMYRK 301 ACVT TADEAV ASCQMIGYPA MIKASWGGGG KGIRKVNNDD EVKALFKQVQ 351 GEVPGSPIFI MRLASQSRHL EVQLLCDEYG NVAALHSRDC SVQRRHQKII 401 EEGPVTVAPR ETVKELEQAA RRLAKAVGYV GAATVEYLYS METGEYYFLE 451 LNPRLQVEHP VTESIAEVNL PAAQVAVGMG IPLWQIPEIR RFYGMDNGGG 501 YDIWRKTAAL ATPFNFDEVD SQWPKGHCVA VRITSENPDD GFKPTGGKVK 551 EISFKSKPNV WGYFSVKSGG GIHEFADSQF GHVFAYGETR SAAITSMSLA 601 LKEIQIRGEI HTNVDYTVDL LNAPDFRENT IHTGWLDTRI AMRVQAERPP 651 WYISWGGAL YKTITTNAET VSEYVSYLIK GQIPPKHISL VHSTISLNIE 701 ESKYTIEIVR SGQGSYRLRL NGSLIEANVQ TLCDGGLLMQ LDGNSHVIYA 751 EEEAGGTRLL IDGKTCLLQN DHDPSRLLAE TPCKLLRFLI ADGAHVDADV 801 PYAEVEVMKM CMPLLSPAAG VINVLLSEGQ AMQAGDLIAR LDLDDPSAVK 851 RAEPFEGSFP EMSLPIAASG QVHKRCAASL NAARMVLAGY DHAANKWQD 901 LVWCLDTPAL PFLQWEELMS VLATRLPRRL KSELEGKYNE YKLNVDHVKI 951 KDFPTEMLRE TIEENLACVS EKEMVTIERL VDPLMSLLKS YEGGRESHAH 1001 FIVKSLFEEY LSVEELFSDG IQSDVIERLR LQYSKDLQKV VDIVLSHQGV 81 WO 2024 / 206966 PCT / US2024 / 022430 1051 RNKTKLILAL MEKLVYPNPA AYRDQLIRFS SLNHKRYYKL ALKASELLEQ 1101 TKLSELRTSI ARNLSALDMF TEEKADFSLQ DRKLAINESM GDLVTAPLPV 1151 EDALVSLFDC TDQTLQQRVI QTYISRLYQP QLVKDSIQLK YQDSGVIALW 1201 EFTEGNHEKR LGAMVILKSL ESVSTAIGAA LKDASHYASS AGNTVHIALL 1251 DADTQLNTTE DSGDNDQAQD KMDKLSFVLK QDWMADLRA ADVKWSCIV 1301 QRDGAIMPMR RTFLLSEEKL CYEEEPILRH VEPPLSALLE LDKLKVKGYN 1351 EMKYTPSRDR QWHIYTLRNT ENPKMLHRVF FRTLVRQPSA GNRFTSDHIT 1401 DVEVGHAEEP LSFTSSSILK SLKIAKEELE LHAIRTGHSH MYLCILKEQK 1451 LLDLVPVSGN TWDVGQDEA TACSLLKEMA LKIHELVGAR MHHLSVCQWE 1501 VKLKLVSDGP ASGSWRWTT NVTGHTCTVD IYREVEDTES QKLVYHSTAL 1551 SSGPLHGVAL NTSYQPLSVI DLKRCSARNN KTTYCYDFPL TFEAAVQKSW 1601 SNISSENNQC YVKATELVFA EKNGSWGTPI IPMQRAAGLN DIGMVAWILD 1651 MSTPEFPSGR QIIVIANDIT FRAGSFGPRE DAFFEAVTNL ACEKKLPLIY 1701 LAANSGARIG IADEVKSCFR VGWTDDSSPE RGFRYIYMTD EDHDRIGSSV 1751 IAHKMQLDSG EIRWVIDSW GKEDGLGVEN IHGSAAIASA YSRAYEETFT 1801 LTFVTGRTVG IGAYLARLGI RCIQRIDQPI ILTGFSALNK LLGREVYSSH 1851 MQLGGPKIMA TNGWHLTVP DDLEGVSNIL RWLSYVPANI GGPLPITKSL 1901 DPIDRPVAYI PENTCDPRAA ISGIDDSQGK WLGGMFDKDS FVETFEGWAK 1951 TWTGRAKLG GIPVGVIAVE TQTMMQLVPA DPGQPDSHER SVPRAGQVWF 2001 PDSATKTAQA MLDFNREGLP LFILANWRGF SGGQRDLFEG ILQAGSTIVE 82 WO 2024 / 206966 PCT / US2024 / 022430 2051 NLRTYNQPAF VYIPKAAELR GGAWWIDSK INPDRIECYA ERTAKGNVLE 2101 PQGLIEIKFR SEELKECMGR LDPELIDLKA RLQGANGSLS DGESLQKSIE 2151 ARKKQLLPLY TQIAVRFAEL HDTSLRMAAK GVIRKWDWE DSRSFFYKRL 2201 RRRLSEDVLA KEIRGVIGEK FPHKSAIELI KKWYLASEAA AAGSTDWDDD 2251 DAFVAWRENP ENYKEYIKEL RAQRVSRLLS DVAGSSSDLQ ALPQGLSMLL 2301 DKMDPSKRAQ FIEEVMKVLK [000101] The O ryza sa tiva ACCase gene product has the following sequence (Swiss- Prot B9FK36, SEQ ID NO: 4; Figure 2B): 10 20 30 40 50 60 MTSTHVATLG VGAQAPPRHQ KKSAGTAFVS SGSSRPSYRK NGQRTRSLRE ESNGGVSDSK 70 80 90 100 110 120 KLNHSIRQGL AGIIDLPNDA ASEVDISHGS EDPRGPTVPG SYQMNGIINE THNGRHASVS 130 140 150 160 170 180 KWEFCTALG GKTPIHSVLV ANNGMAAAKF MRSVRTWAND TFGSEKAIQL IAMATPEDLR 190 200 210 220 230 240 INAEHIRIAD QFVEVPGGTN NNNYANVQLI VEIAERTGVS AVWPGWGHAS ENPELPDALT 250 260 270 280 290 300 AKGIVFLGPP ASSMHALGDK VGSALIAQAA GVPTLAWSGS HVEVPLECCL DSIPDEMYRK 310 320 330 340 350 360 ACVTTTEEAV ASCQWGYPA MIKASWGGGG KGIRKVHNDD EVRTLFKQVQ GEVPGSPIFI 370 380 390 400 410 420 83 W O 2024 / 206966 PCT / US2024 / 022430 MRLAAQSRHL EVQLLCDQYG NVAALHSRDC SVQRRHQKII EEGPVTVAPR ETVKELEQAA 430 440 450 460 470 480 RRLAKAVGYV GAATVEYLYS METGEYYFLE LNPRLQVEHP VTEWIAEVNL PAAQVAVGMG 490 500 510 520 530 540 IPLWQIPEIR RFYGMNHGGG YDLWRKTAAL ATPFNFDEVD SKWPKGHCVA VRITSEDPDD 550 560 570 580 590 600 GFKPTGGKVK EISFKSKPNV WAYFSVKSGG GIHEFADSQF GHVFAYGTTR SAAITTMALA 610 620 630 640 650 660 LKEVQIRGEI HSNVDYTVDL LNASDFRENK IHTGWLDTRI AMRVQAERPP WYISWGGAL 670 680 690 700 710 720 YKTVT ANTAT VSDYVGYLTK GQIPPKHISL VYTTVALNID GKKYTIDTVR SGHGSYRLRM 730 740 750 760 770 780 NGSTVDANVQ ILCDGGLLMQ LDGNSHVIYA EEEASGTRLL IDGKTCMLQN DHDPSKLLAE 790 800 810 820 830 840 TPCKLLRFLV ADGAHVDADV PYAEVEVMKM CMPLLSPASG VIHWMSEGQ AMQAGDLIAR 850 860 870 880 890 900 LDLDDPSAVK RAEPFEDTFP QMGLPIAASG QVHKLCAASL NACRMILAGY EHDIDKWPE 910 920 930 940 950 960 LVYCLDTPEL PFLQWEELMS VLATRLPRNL KSELEGKYEE YKVKFDSGII NDFPANMLRV 970 980 990 1000 1010 1020 IIEENLACGS EKEKATNERL VEPLMSLLKS YEGGRESHAH FWKSLFEEY LYVEELFSDG 1030 1040 1050 1060 1070 1080 IQSDVIERLR LQHSKDLQKV VDIVLSHQSV RNKTKLILKL MESLVYPNPA AYRDQLIRFS 84 W O 2024 / 206966 1090 1100 1110 1120 1130 PCT / US2024 / 022430 1140 SLNHKAYYKL ALKASELLEQ TKLSELRARI ARSLSELEMF TEESKGLSMH KREIAIKESM 1150 1160 1170 1180 1190 1200 EDLVTAPLPV EDALISLFDC SDTTVQQRVI ETYIARLYQP HLVKDSIKMK WIESGVIALW 1210 1220 1230 1240 1250 1260 EFPEGHFDAR NGGAVLGDKR WGAMVIVKSL ESLSMAIRFA LKETSHYTSS EGNMMHIALL 1270 1280 1290 1300 1310 1320 GADNKMHIIQ ESGDDADRIA KLPLILKDNV TDLHASGVKT ISFIVQRDEA RMTMRRTFLW 1330 1340 1350 1360 1370 1380 SDEKLSYEEE PILRHVEPPL SALLELDKLK VKGYNEMKYT PSRDRQWHIY TLRNTENPKM 1390 1400 1410 1420 1430 1440 LHRVFFRTLV RQPSVSNKFS SGQIGDMEVG SAEEPLSFTS TSILRSLMTA IEELELHAIR 1450 1460 1470 1480 1490 1500 TGHSHMYLHV LKEQKLLDLV PVSGNTVLDV GQDEATAYSL LKEMAMKIHE LVGARMHHLS 1510 1520 1530 1540 1550 1560 VCQWEVKLKL DCDGPASGTW RIVTTNVTSH TCTVDIYREM EDKESRKLVY HPATPAAGPL 1570 1580 1590 1600 1610 1620 HGVALNNPYQ PLSVIDLKRC SARNNRTTYC YDFPLAFETA VRKSWSSSTS GASKGVENAQ 1630 1640 1650 1660 1670 1680 CYVKATELVF ADKHGSWGTP LVQMDRPAGL NDIGMVAWTL KMSTPEFPSG REIIWANDI 1690 1700 1710 1720 1730 1740 TFRAGSFGPR EDAFFEAVTN LACEKKLPLI YLAANSGARI GIADEVKSCF RVGWSDDGSP 1750 1760 1770 1780 1790 1800 85 PCT / US2024 / 022430WO 2024 / 206966 ERGFQYIYLS EEDYARIGTS VIAHKMQLDS GEIRWVIDSV VGKEDGLGVE NIHGSAAIAS 1810 1820 1830 1840 1850 1860 AYSRAYKETF TLTFVTGRTV GIGAYLARLG IRCIQRLDQP IILTGYSALN KLLGREVYSS 1870 1880 1890 1900 1910 1920 HMQLGGPKIM ATNGWHLTV SDDLEGVSNI LRWLSYVPAY IGGPLPVTTP LDPPDRPVAY 1930 1940 1950 1960 1970 1980 IPENSCDPRA AIRGVDDSQG KWLGGMFDKD SFVETFEGWA KTWTGRAKL GGIPVGVIAV 1990 2000 2010 2020 2030 2040 ETQTMMQTIP ADPGQLDSRE QSVPRAGQVW FPDSATKTAQ ALLDFNREGL PLFILANWRG 2050 2060 2070 2080 2090 2100 FSGGQRDLFE GILQAGSTIV ENLRTYNQPA FVYIPMAAEL RGGAWVWDS KINPDRIECY 2110 2120 2130 2140 2150 2160 AERTAKGNVL EPQGLIEIKF RSEELQDCMS RLDPTLIDLK AKLEVANKNG SADTKSLQEN 2170 2180 2190 2200 2210 2220 IEARTKQLMP LYTQIAIRFA ELHDTSLRMA AKGVIKKWD WEESRSFFYK RLRRRISEDV 2230 2240 2250 2260 2270 2280 LAKEIRAVAG EQFSHQPAIE LIKKWYSASH AAEWDDDDAF VAWMDNPENY KDYIQYLKAQ 2290 2300 2310 2320 RVSQSLSSLS DSSSDLQALP QGLSMLLDKM DPSRRAQLVE EIRKVLG [000102] Some species have more than one ACCase gene. In such a case one or more of the genes are mutated according to the present description to make herbicide resistant mutant. If the expression levels of the various ACCase genes is known and is different, then it is preferred to mutate the higher expressing ACCase genes. In a preferred embodiment all of the ACCase genes in a crop are mutated to make a one or more of 86 WO 2024 / 206966 PCT / US2024 / 022430 alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, diclofop, fenoxaprop, fenoxaprop-P, fenthiaprop, fluazifop, fluazifop-P, haloxyfop, haloxyfop-P, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P, trifop, pinoxaden, agronomically acceptable salts and esters of any of these herbicides, and combinations thereof phenotype. [000103] Mutations and mutant ACCase genes that will produce a gene product that provides resistance to one or more herbicides can be identified, for example, using techniques known in the art. For example, in vitro screening can be done in a microbial system to save time and resources. Growth curves of microbial colonies expressing candidate mutant ACCase genes can be generated to evaluate the mutant ACCase genes in providing a phenotype having resistance to one or more herbicides. For example, U.S. Patent 6,306,636 discloses a Saccharomyces cerevisiae ACC1 mutant complemented with a chimeric wheat cytosolic ACCase construct to identify one or more of alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, diclofop, fenoxaprop, fenoxaprop-P, fenthiaprop, fluazifop, fluazifop-P, haloxyfop, haloxyfop-P, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P, trifop, pinoxaden, agronomically acceptable salts and esters of any of these herbicides, and combinations thereof resistance assay employing Oryza mutant ACCase genes transformed into a Saccharomyces cerevisiae strain lacking the native accl gene. Growth curve assays and enzymatic assays measuring Ki and Km values for candidate mutants are conducted according to well-known assay techniques. [000104] In various aspects and embodiments of the present disclosure, provided include a plant having one or more ACCase mutations and / or mutation combinations selected from the group consisting of the mutations / combinations listed on Table 1 below and / or methods of making such a plant. As denoted at the bottom of Table 1, some amino acids in certain ACCase proteins (e .g ., the rice plastidal ACCase protein) are not found in the blackgrass (Alopecurus myosuroides) protein sequence. These positions have been noted with a double number identification that indicates the surrounding positions in the blackgrass (.Alopecurus myosuroides) protein. By way of example “rice G1605-6A*” refers to a glycine residue in the rice ACCase protein that lies between residues 1605 and 1606 of the blackgrass {Alopecurus myosuroides) ACCase sequence and has been substituted with an alanine residue. [000105] Table 1. Exemplary mutations; position numbering is rcited relative to blackgrass {Alopecurus myosuroides) plastidal ACCase positions (Figure IB; SEQ ID NO. 87 WO 2024 / 206966 PCT / US2024 / 022430 2). By way of example, an N-to-S substitution at postion 1460 of SEQ ID NO: 2 may be written as “N —>■ S” or “N1460S.” ACCase Position Specific Changes and / or Specific Combinations 1460 N~> S 1529 V —» M 1529 V1529M + L1818H 1529 V1529M + L1818I 1543 L F 1543 L -> V 1543 L1543F +D1681E 1543 L1543F + D1681G 1543 L1543V + D1681E 1543 L1543V + D1681G 1555 L P 1555 L1555P + S1605Y + D2078G 1575 C - > S 1575 C1575S + G1605-6A* + V2075L 1605 S —> Y 1605 S1605Y + V2098A 1605 S1605Y + L1555P + D2078G 1605-6* <tO 1605-6* G1605-6A* + C1575S + V2075L 1607 N — S 1649 L - > M 1649 L1649M + V1715A + D2078G 1672 R —» I 1672 R1672I + D2078G 1672 R1672I + M1975T + D2078G 1681 D E 1681 D ^ G 1681 D 168 IE + L1543V 1681 D1681E + L1543F 88 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 1681 D1681G + L1543V 1681 D1681G + L1543F 1683 ! •' > L 1683 F1683L + A1752T + K 193 ON + V2Q75L + E2169D 1715 V —» A 1715 V1715A + L1649M + D2078G 1725 D — E 1725 D1725E + 1178 IT + V2075L 1734 R > G 1738 M - > L 1739 T -> S 1752 A —> T 1752 A1752T + F1683L + K 193 ON + V2075L + E2169D 1756 Q P 1756 q > [ •: 1766 I - > V 1766 11766V + D2078G + D2127N 1772 K E 1772 K ~ > N 1772 K1772E+ S1784G 1772 K1772E + S1784N 1772 K1772E+ S1784G 1772 K1772E+ S1784N 1780 N ~ > D 1780 N1780D + I1781L 1781 ! > S, 1781 I - > T 1781 I - >V 1781 I A 1781 11781L + N1780D 1781 I1781L +A1785G 1781 I1781L + Q1824P 89 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 1781 I1781L + V1864F 1781 I1781L +W 1999G 1781 I1781L + W2027C 1781 I1781L + W2027R 1781 I1781L +1204 IN 1781 I1781L +V2049F 1781 I1781L +V2075L 1781 11781L + D2078G 1781 L1781L + C2088M 1781 117 81L + C2088R 1781 I 1781L +V2098G 1781 117 81L + A1785G + C2088M 1781 I1781T +V2075L 1781 1178 IT + D1725E + V2075L 1783 G -> C 1784 S G 1784 S - > N 1784 S1784G + L1818H 1784 S1784G + LI 8181 1784 S1784N + L1818H 1784 S1784N + LI 8181 1784 S1784G + K1772E 1784 S1784N + K1772E 1785 A —► G 1785 A1785G + I1781L 1785 A1785G + D2078G 1785 A1785G +Y2098A 1785 A1785G + V2098G 1785 I1781L + A1785G + C2088M 1786 A —> P 1792 S ^ L 1792 S1792L + Y2049F 90 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 1811 I --> N 1818 L H 1818 L - > I 1818 L1818H +V1534M 1818 L1818H + K184QM 1818 L1818H + K1840R 1818 L1818H+ S1784N 1818 L1818H+ S1784G 1818 L1818I + S1784N 1818 LI 8181 + S1784G 1818 L1818I +V1534M 1818 LI 8181 + K1840M 1818 L1818I + K1840R 1824 Q ^ P 1824 Q1824P + I1781L 1824 Q1824P + D2078G 1837 A —► V 1837 A1837V +V2075L 1837 A1837V +V2075I 1840 K -> R 1840 K —»• M 1840 K1840R + L1818H 1840 K1840M + L1818H 1840 K1840R + LI 8181 1840 K1840M + LI 8181 1864 V —» F 1864 V1864F + I1781L 1864 V1864F + W1999G 1870 P —>■ R 1922 S > G 1930 K -»• N 1930 K1930N + Al 7521 + F1683L + V2075L + E2169D 91 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 1938 K -> R 1946 E G 1946 E —>D 1946 E - > V 1946 El 946V + D2078G + A2179T + F2253I 1957 A > T 1957 A1957T +V2075L 1966 V —»■ L 1966 V1966L + D2078G + V2182A + K2317T 1967 I - > V 1967 11967V + V2075L 1974 M - * T 1974 M l 9741 + D2078G 1975 M -»• T 1975 M1975T + D2078G 1975 M1975T + R1672I + D2078G 1992 V —» D 1992 V1992D + V2075L + F2196Y + L2299I 1999 W - > C 1999 W --> G 1999 W ^ L 1999 w --> s 1999 W1999C + V2075I 1999 W1999G + I1781L 1999 W1999G + V1864F 1999 W1999G + V2049I 1999 W1999G + V2075L 1999 W 1999G + D2078G 1999 W1999G + G2096A 1999 W1999G +V2098A 1999 W1999L +V2049F 2004 A —> V 92 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2112 E > V 2112 E2112 V + D2078G 2027 W ->C 2027 W —> R 2027 W2027R + I1781L 2027 W20207R + I1781L 2039 E -> D 2039 E G 2039 E2039G + D2078G 2041 I - > D 2041 I —> N 2041 I - > V 2041 1204 IN + I1781L 2041 1204 IN + D2078G 2041 12041V + D2078G 2041 1204 IN + C2088R 2049 V —»• F 2049 V - + I 2049 V —> L 2049 V —> T 2049 v - > c 2049 V > A 2049 v -> s 2049 V2049F + I1781L 2049 V2049F +11781V 2049 V2049F + S1792L 2049 V2049I + W1999G 2049 V2049F + W1999L 2049 V2049F + C2088F 2049 V2049A + D2078G or V2098A 2049 V2049C + D2078G or V2098A 2049 V2049L + D2078G or V2098A 93 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2049 V2Q49S + D2078G or V2Q98A 2049 V2049T + D2078G or V2098A 2049 V2049I + V2075L + A2178I 2059 A —» V 2059 A2059G + D2078G 2059 A2059V + D2078G + S228QP + S2297C 2067 A S 2067 A2067S + V2075L + L2159P 2074 W ^ L 2075 V —» M 2075 V - > L 2075 V - + I 2075 V —>■ G 2075 V2075L + C1575S + G1605-6A* 2075 V2075L+ 1781L 2075 V2075L + I1781T 2075 V2075I + A1837V 2075 V2075I + W1999C 2075 V2075L + W1999G 2075 V2075M + K2080E 2075 V2075L + C2088W 2075 V2075L + N2097S 2075 V2075L + II967V 2075 V2075L + D1725E + I1781T 2075 V2075L + K 193 ON + A1752T + F1683L + E2169D 2078 D —> G 2078 D~ > K 2078 D —> T 2078 D -^ V 2078 D2078G + I1249K 2078 D2078G + G1605-6A* 2078 D2078G + I1781L 94 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2078 D2078G + I1785G 2078 D2078G + Q1824P 2078 D2078G +W 1999G 2078 D2078G + E2039G 2078 D2078G +1204 IN 2078 D2078G +12041V 2078 D2078G + V2049A / C / L / S / T 2078 D2078G + A2059G 2078 D2078G+ S2079F 2078 D2078G+ S2079P 2078 D2078G + P2083T 2078 D2078G + C2088G 2078 D2078G + C2088N 2078 D2078G + N2097S 2078 D2078G + E2112V 2078 D2078G + N2262K 2078 D2078G + I2267V + E2150K 2078 D2078G + S2276P + K2302Q 2078 D2078G + L1555P + S1605Y 2078 D2078G + L1649M + V1715A 2078 D2078G + R 16721 2078 D2078G + R1672I + M1975T 2078 D2078G + 11766V + D2127N 2078 D2078G + E l 946V + A2179T + F2253I 2078 D2078G + M1974T 2078 D2078G+ G1605-6A* 2078 D2078G + V1966L + V2182A + K2317T 2078 D2078G + A2059V + S2280P + S2297C 2078 D2078G + R2085S + Q2155L 2078 D2078G + N2097S + H2223R + S2276F 2078 D2078G + K2154R + K2232N 2078 D2078G + I2149K 95 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2079 S > F 2079 S - + P 2079 S2079F + D2078G 2079 S2079P + D2078G 2080 k > ! •: 2080 K —»■ deleted 2080 K2080E + V2075M 2081 I deleted 2081 2080 deleted + 2081 deleted 2083 P —» N 2083 I O 2083 P~> s 2083 P - * T 2083 P2083T + D2078G 2085 R » S 2085 R2085S + D2078G + Q2155L 2088 C —► R 2088 n i s? 2088 ( > 1 2088 C G 2088 C > H 2088 C > K 2088 c --> s 2088 C - > T 2088 C - ^ L 2088 C~> V 2088 C —> N 2088 C M 2088 C2088R + I1781L 2088 C2088R + 1204 IN 2088 C2088F + V2049F 2088 C2088W + V2075L 96 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2088 C2088G + D2078G 2088 C2088R+ D2078G 2088 C2088W + V2098C 2088 C2088T + V2098A 2088 C2088G + V2098G 2088 C2088H+ V2098A 2088 C2088H+ V2098G 2088 C2088K + V2098A 2088 C2088L + V2098A 2088 C2088L + V2098G 2088 C2088S + V2098G 2088 C2088V+ V2098G 2088 C2088M + I1781L 2088 C2088M + 11781L + A1785G 2088 C2088N + D2078G 2095 K —► E 2095 K -» N 2095 K2095E + V2075M 2096 G~> A 2096 G~> S 2096 G2096A + V2Q98A 2096 G2096A +W 1995G 2097 N -> R 2097 N -» K 2097 N -> E 2097 N —»■ H 2097 N —►D 2097 N —> A 2097 N —> G 2097 N - ^ C 2097 N V 2097 N ^ P 97 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2097 N - > L 2097 N --> I 2097 N —► M 2097 N F 2097 N —> W 2097 N —> Q 2097 N — S 2097 N -»• T 2097 N Y 2097 N2097R + V2075L or V2075I 2097 N2097K + V2075L or V2075I 2097 N2097E + V2075L or V2075I 2097 N2097H + Y2075L or V2075I 2097 N2097D + V2075L or V2075I 2097 N2097A + V2075L or V2075I 2097 N2097G + V2075L or V2075I 2097 N2097C + V2075L or V2075I 2097 N2097V + Y2075L or V2075I 2097 N2097P + V2075L or V2075I 2097 N2097L + V2075L or V2075I 2097 N2097I + V2Q75L or V2075I 2097 N2097M + V2075L or V2075I 2097 N2097F + V2075L or V2075I 2097 N2097W + V2075L or V2075I 2097 N2097Q + V2075L or V2075I 2097 N2097S + V2075L or V2075I 2097 N2097T + V2075L or V2075I 2097 N2097Y + V2075L or V2075I 2097 N2097R + D2078G 2097 N2097K + D2078G 2097 N2097E + D2078G 2097 N2097H + D2078G 98 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2097 N2097D + D2078G 2097 N2097A + D2078G 2097 N2097G + D2078G 2097 N2097C + D2078G 2097 N2097V + D2078G 2097 N2097P + D2078G 2097 N2097L + D2078G 2097 N2097I + D2078G 2097 N2097M + D2078G 2097 N2097F + D2078G 2097 N2097W + D2078G 2097 N2097Q + D2078G 2097 N2097S + D2078G 2097 N2097T + D2078G 2097 N2097Y + D2078G 2097 N2097R + V2098A or V2098G 2097 N2097K + V2Q98A or V2098G 2097 N2097E + V2098A or V2098G 2097 N2097H + V2098A or V2098G 2097 N2097D + V2098A or V2098G 2097 N2097A + V2098A or V2098G 2097 N2097G + V2098A or V2098G 2097 N2097C + V2098A or V2098G 2097 N2097V + V2098A or V2098G 2097 N2097P + V2098A or V2098G 2097 N2097L + V2098A or V2098G 2097 N2097I + V2098A or V2098G 2097 N2097M + V2098A or V2098G 2097 N2097F + V2098A or V2098G 2097 N2097W + V2098A or V2098G 2097 N2097Q + V2098A or V2098G 2097 N2097S + V2Q98A or V2098G 99 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2097 N2097T + V2098A or V2098G 2097 N2097Y + V2Q98A or V2098G 2097 N2Q97S + D2078G + H2223R + S2276F 2098 V —» A 2098 V —»■ H 2098 v ~ > p 2098 s 2098 V > G 2098 V -> c 2098 V2098G + I1781L 2098 V2098A +A1785G 2098 V2098G + N2087S 2098 V2098A + N2087S 2098 V2098G + A1785G 2098 V2098A + W1999G 2098 V2098C + C2088W 2098 V2098A + C2088F / H / K / L / T 2098 V2098G + C2088G / H / L / S / T / V 2098 V2098A + G2096A 2098 V2098A + A2151T + V2254I 2098 V2098A + V2049A / C / L / S / T 2098 V2098G + A2226S 2112 E -> G 2112 E -> V 2112 E2112G + D2078G 2112 E2112V + D2078G 2127 D - + N 2127 D2127N + I1766V + D2078G 2149 I —> K 2149 I V 2149 I2149K + D2078G 2150 E -> K 100 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2150 E2150K + D2078G + 12267V 2154 K -> R 2154 K2154R + D2078G + K2232N 2155 Q -> L 2155 Q2155L + R2085S + D2078G 2169 E -> D 2169 V2075L + K 193 ON + A1752T + F1683L + E2169D 2179 A -»• T 2179 A2179T + E l 946V + D2078G + F2253I 2182 V —>• A 2182 V2182A + V1966L + D2078G + K2317T 2223 II -> R 2223 H2223R + D2078G + N2097S + S2276F 2232 K -> N 2232 K2232N + D2078G + K2154R 2241 A - > T 2253 i > 1 2253 F2253I + A2179T + El 946V + D2078G 2262 N —> K 2262 N2262K + D2078G 2264 K ^ R 2267 I - > V 2267 I2267V + D2078G + N2097S + H2223R 2276 S - ^ P 2276 S > !• 2276 S2276P + D2078G + K2302Q 2276 S2276F + H2223R + D2078G + N2097S 2280 S »P 2280 A2059V + D2078G + S2280P + S2297C 2297 S ^ C 2297 S2297C + A2059V + D2078G + S2280P 2302 I O 101 WO 2024 / 206966 PCT / US2024 / 022430 ACCase Position Specific Changes and / or Specific Combinations 2302 K2302Q + D2078G + S2276P 2317 K —> T 2317 K2317T + V1966L + D2078G + V2182A *G1605-6 denotes a residue found in the rice ACCase enzymes but not in the blackgrass (A lopecurus m yosuroides) reference sequence. The rice G 1605-6 residue aligns between positions 1605 and 1606 of the blackgrass (.A lopecurus m yosuroides) enzyme. [000106] In various aspects and embodiments of the present disclosure, provided include a plant having one or more ACCase mutations and / or mutation combinations selected from the group consisting of the mutations / combinations listed on Table 2 below and / or methods of making such a plant. [000107] Table 2, Exemplary mutations relative to blackgrass {A lopecurus m yo su ro id es) plastidal ACCase positions (Figure IB; SEQ ID NO. 2) Mutation(s) Alternative Substitution(s) L1543F + D1681G L 1543 V / R / K / E / H / D / A / G / C7P / I / M / W / Q / T / Y / S / N; D 1681E / V / L / F / R / K / H / A / C / P / I / M / W / Q / T / Y / S / N A1785G + I1781L A 178 5R / K / E / H / D / C / V / P / L / I / M / F / W / Q / T / Y / S / N; I1781A / M / N / S / T / V A1785G + C2088M A 178 5R / K / E / H / D / C / V / P / L / I / M / F / W / Q / T / Y / S / N; C2088F / G / H / K / L / P / Q / R / S / T / V / W / N L1818H+ S1784N L 1818I / R / K / E / D / A / G / C / V / P / M / F / W / Q / T / Y / S / N; S1784G / R / K / E / H / D / A / C / V / P / L / I / M / F / W / Q / T / Y L1818H +V1529M L 1818I / R / K / E / D / A / G / C / V / P / M / F / W / Q / T / Y / S / N; VI529R / K / E / H / D / A / G / C / P / L / I / F / W / Q / T / Y / S / N L1818H + K1840R L 1818I / R / K / E / D / A / G / C / V / P / M / F / W / Q / T / Y / S / N; K 1840M / E / H / D / A / G / C / V / P / L / I / F / W / Q / T / Y / S / N V2075L + N2097S V2075I; N2097R / K / E / H / D / A / G / C / V / P / L / I / M / F / W / Q / T / Y 102 WO 2024 / 206966 PCT / US2024 / 022430 Mutation(s) Alternative Substitution(s) V2075L + I1781T V2075I; I1781A / M / N / S / L / V D2078G + N2097S D2078K / T; N2097R / K / E / H / D / A / G / C / V / P / L / I / M / F / W / Q / TA7 D2078G + P2083T D2078K / T; P2083N / Q / S D2078G + C2088N D2078K / T; C2088F / G / H / K / L / P / Q / R / S / T / V / W / M D2078G + R1672I D2078K / T; R1672K / E / H / D / A / G / C / V / P / L / M / F / W / Q / T / Y / S / N D2078G + M1974T D2078K / T; M 1974R / K / E / H / D / A / G / C / V / P / L / I / F / W / Q / Y / S / N V2098A + N2097S N2097R / K / E / H / D / A / G / C / V / P / L / I / M / F / W / Q / TA7 V2098G + N2097S N2097R / K / E / H / D / A / G / C / V / P / L / I / M / F / W / Q / TA7 V1529M VI529R / K / E / H / D / A / G / C / P / L / I / F / W / Q / T / Y / S / N L1543F L 1543V / R / K / E / H / D / A / G / C / P / I / M / W / Q / T / Y / S / N D1681G D 1681E / V / L / F / R / K / H / A / C / P / I / M / W / Q / T / Y / S / N L1818H L 1818I / R / K / E / D / A / G / C / V / P / M / F / W / Q / T / Y / S / N K1840R K 1840M / E / H / D / A / G / C / V / P / L / I / F / W / Q / T / Y / S / N RI 6721 R1672K / E / H / D / A / G / C / V / P / L / M / F / W / Q / T / Y / S / N I1781L I1781A / M / N / S / T / V I1781T I1781A / M / N / S / L / V S1784N S1784G / R / K / E / H / D / A / C / V / P / L / I / M / F / W / Q / T / Y A1785G A 178 5R / K / E / H / D / C / V / P / L / I / M / F / W / Q / T / Y / S / N M1974T M 1974R / K / E / H / D / A / G / C / V / P / L / I / F / W / Q / Y / S / N V2075L V2075I D2078G D2078K / T 103 WO 2024 / 206966 PCT / US2024 / 022430 Mutation(s) Alternative Substitution(s) P2083T P2083N / Q / S C2088N C208 8R / K / E / H / D / A / G / V / P / L / I / M / F / W / Q / T / Y / S N2097S N2097R / K / E / H / D / A / G / C / V / P / L / I / M / F / W / Q / T / Y V2098A V2098G [000108] In various aspects and embodiments of the present disclosure, provided include a plant having one or more ACCase mutations and / or mutation combinations selected from the group consisting of the mutations / combinations listed on Table 3 below and / or methods of making such a plant. [000109] Table 3, Exemplary mutations relative to blackgrass (,A lopecurus m yo su ro id es) plastidal ACCase positions (Figure IB; SEQ ID NO. 2) Mutation(s) V1529M + L1818H L1555P + S1605Y + L1985P + D2078G C1575S + G1605-6A* + V2075L S1602F + D1725E + I1781T + V2075L S1603N + L1649M + V1715A + D2078G S1605Y +V2098A A1957T +V2075L V1966L + D2078G + V2182A + A2244T + K2317T Il 967V + V2075L + S2119C R1672I + M1975T + D2078G R1672I + D2078G F1683L + A1752T + K1930N + V2075L + E2169D I1766V + D2078G + D2127N K1772E + S1784N + V1924D E l 946V + D2078G + A2179T + F2253I M1974T+ D2078G + M2257I M1974T+ D2078G M1975T + D2078G V1992D + V2075L + F2196Y + L2299I 104 WO 2024 / 206966 PCT / US2024 / 022430 Mutation(s) V2049I + V2075L + A2178I A2059V + D2078G + S2280P + S2297C A2059V + D2078G + S2280P + S2297C M2065K + D2078G + R2085S + Q2155L + Q2220L A2067S + V2075L + L2159P V2075L + N2097S D2078G D2078G + P2083T D2078G + N2097S + H2223R + S2276F D2078G + E2112V D2078G + I2149K D2078G + E2150K + I2267V D2078G + K2154R + K2232N D2078G + M2257I D2078G + N2262K D2078G + S2276P + K2302Q V2098A + V2134I + A2151T + V2254I V2098G V2098G+ A2226S [000110] In some embodiments the plant or plant cell includes a mutated ACCase gene, wherein the gene encodes a protein including a mutation at one or more amino acid positions corresponding to a position or position combinations selected from the group consisting of L1543V / D1681E; L1543F / D1681E; L1543V / D1681G; L1543F / D1681G; A1785G / I1781L; A1785G / I1781T; A1785G / I1781V; A1785G / C2088M; A1785G / C2088N; A1785G / C2088N; A1785G / C2088R; L1818H / S1784N; L1818H / S1784G: L1818I / S1784N: L1818I / S1784G: V1529M / L1818H: V1529M / L1818I: K1840R / L1818H; N2097D / D2078G; N2097D / V2075I; N2097S / V2098A; D2078G / P2083T; K1840R / L1818I; N2097S / D2078G; N2097S / V2075L; N2097S / V2098G; D2078G / C2088M; K1840M / L1818H; N2097D / V2075L; N2097D / V2098A; R1672I / D2078G; D2078G / C2088N; K1840M / L1818I; N2097S / V2075I; N2097D / V2098G; M1974T / D2078G; D2078G / C2088R; 105 WO 2024 / 206966 PCT / US2024 / 022430 K1840R / L1818H; S1784N / L1818H, D1725E / I1781T7V2075L; I1781T / V2075L; I1781L / V2075I; I1781L / V2075L; I1781T / V2075I; and I1781V / V2075I; I1781V / V2075L; I1781V / V2075I; and I1781V / V2075L of SEQ ID NO: 2. [000111] In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1543V / D1681E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1543F / D1681E of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1543V / D1681G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1543F / D1681G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1785G / I1781L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1785G / I1781T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1785G / I1781V of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1785G / C2088M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1785G / C2088M of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1785G / C2088N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1785G / C2088R of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a 106 WO 2024 / 206966 PCT / US2024 / 022430 mutation at an amino acid position(s) corresponding to L1818H / S1784N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1818H / S1784G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1818I / S1784N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1818I / S1784G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V1529M / L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V1529M / L1818I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K1840R / L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K1840R / L1818I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K1840M / L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K1840M / L1818I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to N2097S / D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to N2097S / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to N2097S / V2098A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene 107 WO 2024 / 206966 PCT / US2024 / 022430 wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to N2097S / V2098G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to R1672I / D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to M1974T / D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D2078G / P2083T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D2078G / C2088N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K1840R / L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to S1784N / L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to S1784N / L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D1725E / I1781T / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1781T / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1781L / V2075Iof SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1781L / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1781T / V2075I of SEQ ID NO: 2. In certain embodiments, a plant or 108 WO 2024 / 206966 PCT / US2024 / 022430 plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1781V / V2075I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1781V / V2075L of SEQ ID NO: 2. [000112] In some embodiments the plant or plant cell includes a mutated ACCase gene, wherein the gene encodes a protein including a mutation at one or more amino acid positions corresponding to a position or position combinations selected from the group consisting of D1725E / I1781T / V2075L, V2098G, A2059V / D2078G / S2280P / S2297C, E2112V / D2078G, D1681G, D2078G / R1672I / M1975T, H2223R / D2078G / N2097S / S2276F, C1575S / G1605-6AW 2075L, D2078G / S2276P / K2302Q, R2085S / D2078G / Q2155L, M1974T / D2078G, D2078G / I2267V / E2150K, K 1930N / A1752T7F1683L / V2075L / E2169D, 11967V / V2075L, K1772E / S1784G, K2154R / D2078G / K2232N, I2149K / D2078G, A2179T / E1946V / D2078G / F2253I, S1605Y / V2098A, V1529M / L1818H, V 1992D / V2075L / F2196Y / L2299I, V2049EV2075L / A2178I, V2075L / N2097S, V2098A / A2151T / V2254I, L1649M / V1715A / D2078G, L1555P / S1605Y / D20786G, I1766V / D2078G / D2127N, D2078G, D2078G / P2083T, V1966L / D2078G / V2182A / K2317T, A2067S / V2075L / L2159P, V2098G / A2226S, A1957T / V2075L, and N2262K / D2078G of SEQ ID NO: 2. [000113] In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D1725E / I1781T / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V2098G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A2059V / D2078G / S2280P / S2297C of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to E2112V / D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D1681G of SEQ ID NO: 2. In 109 WO 2024 / 206966 PCT / US2024 / 022430 certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D2078G / R1672I / M1975T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to H2223R / D2078G / N2097S / S2276F of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to C1575S / G1605- 6A* / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D2078G / S2276P / K2302Q of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to R2085S / D2078G / Q2155L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to M1974T / D2078G 0f s e q jq NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D2078G / I2267V / E2150K of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K1930N / A1752T7F1683L / V2075L / E2169D of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1967V / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K1772E / S1784G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to K2154R / D2078G / K2232N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I2149K / D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an no WO 2024 / 206966 PCT / US2024 / 022430 ACCase protein including a mutation at an amino acid position(s) corresponding to A2179TVE1946V / D2078G / F2253I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to S1605Y / V2098A of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V1529M / L1818H of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V1992D / V2075L / F2196Y / L2299I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V2049I / V2075L / A2178I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V2075L / N2097S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V2098A / A2151T / V2254I of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1649M / V1715A / D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to L1555P / S1605Y / D20786G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to I1766V / D2078G / D2127N of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to D2078G of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V1966L / D2078G / V2182A / K2317T of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) i l l WO 2024 / 206966 PCT / US2024 / 022430 corresponding to A2067S / V2075L / L2159P of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to V2098G / A2226S of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to A1957T / V2075L of SEQ ID NO: 2. In certain embodiments, a plant or plant cell may include a mutated ACCase gene wherein the gene encodes an ACCase protein including a mutation at an amino acid position(s) corresponding to N2262K / D2078G of SEQ ID NO: 2. [000114] In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein A1785G enhances the herbicide tolerance of an I1781L mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein A1785G enhances the herbicide tolerance of an S1784G mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein A1785G enhances the herbicide tolerance of an S1784N mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein an A1785G mutation enhances the herbicide tolerance of a D2078G mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein an A1785G mutation enhances the herbicide tolerance of an C2088M mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein an N2097S mutation enhances the herbicide tolerance of a D2078G mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein a N2097S mutation enhances the herbicide tolerance of a V2075L mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein a N2097S mutation enhances the herbicide tolerance of a V2098A mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein a N2097S mutation enhances the herbicide tolerance of a V2098G mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein a R1672I mutation enhances the herbicide tolerance of a D2078G mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein a M1974T mutation enhances the herbicide tolerance of a D2078G mutation. In some embodiments provided is a plant or a plant cell having an 112 WO 2024 / 206966 PCT / US2024 / 022430 ACCase gene with one or more mutations wherein a P2083T mutation enhances the herbicide tolerance of a D2078G mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein a K1840R mutation enhances the herbicide tolerance of a L1818H mutation. In some embodiments provided is a plant or a plant cell having an ACCase gene with one or more mutations wherein an I1781T mutation enhances the herbicide tolerance of a V2075L mutation. In each case, the numbering of the ACCase is relative to SEQ ID NO: 2 [000115] In certain embodiments, a plant or plant cell comprises at least two mutations in an ACCase gene such that the mutated gene encodes a protein comprising a first substitution at a position corresponding to D2078 of SEQ ID NO: 2 and a second substitution at a position corresponding to P2083 of SEQ ID NO: 2. Preferably, the plant or plant cell is not transgenic with respect to the D2078 / P2083 substitutions (e.g ., the mutations creating these substitutions are introduced through a gene editing procedure). As noted herein, SEQ ID NO: 2 is used herein as a reference sequence for other homologous ACCase sequences. Thus, the phrase “substitution corresponding to <amino acid substitution at a specified location> in SEQ ID NO: 2” as used herein refers to the same substitution at a corresponding amino acid location in a homologous ACCase protein sequence. By way of example, “an ACCase protein comprising a substitution corresponding to D2078G of SEQ ID NO: 2” includes a D2076G substitution in SEQ ID NO: 6 (O ryza sa tiv a subsp. Ja p ó n ica A C C a se ) and a D2083G substitution in SEQ ID NO: 22 (,Sorghum bico lor). Exemplary D2078 / P2083 amino acid positions compared across species are shown, but not limited to, those listed below: Sequence No. Species Substitution 1 Position Substitution 2 Position 1 (SEQ ID NO: 2) A lo p ecu ru s m yo su ro id es D2078 P2083 2 (SEQ ID NO: 970) A v e n a sa tiva D2078 P2083 3 (SEQ ID NO: 972) A v e n a sa tiva D2078 P2083 4 (SEQ ID NO: 974) A v e n a sa tiva D2078 P2083 5 (SEQ ID NO: 23) B ra ch yp o d iu m d ista ch yo n D2090 P2095 6 (SEQ ID NO: 956) H o rd eu m vu lgare subsp. vu lgare D2066 P2071 113 WO 2024 / 206966 PCT / US2024 / 022430 7 (SEQ ID NO: 966) L o liu m p e ren n e D2076 P2081 8 (SEQ ID NO: 954) O ryza sa tiv a subsp. Ja p ó n ica D2089 P2094 9 (SEQ ID NO: 6) O ryza sa tiv a subsp. Ja p ó n ica D2076 P2081 10 (SEQ ID NO: 4) O ryza sa tiv a cu ltiva r C ypress D2089 P2094 11 (SEQ ID NO: 976) P o a p ra te n s is D2078 P2083 12 (SEQ ID NO: 978) P o a p ra te n s is D2078 P2083 13 (SEQ ID NO: 968) P a n icu m virga tum D2077 P2082 14 (SEQ ID NO: 22) S o rg h u m b ico lo r D2083 P2088 15 (SEQ ID NO: 953) S o rg h u m b ico lo r D2083 P2088 16 (SEQ ID NO: 16) S e ta ria itá lica D2077 P2082 17 (SEQ ID NO: 18) S e ta ria itá lica D2077 P2082 18 (SEQ ID NO: 20) S e ta ria itá lica D2077 P2082 19 (SEQ ID NO: 964) S e ta ria itá lica D2078 P2083 20 (SEQ ID NO: 8) T riticum aestivu m D2066 P2071 21 (SEQ ID NO: 958) T riticum aestivu m D2066 P2071 22 (SEQ ID NO: 960) T riticum aestivu m D2066 P2071 23 (SEQ ID NO: 962) T riticum aestivu m D2066 P2071 24 (SEQ ID NO: 10) Z ea m a ys D2080 P2085 25 (SEQ ID NO: 12) Z ea m a ys D2081 P2086 26 (SEQ ID NO: 14) Z ea m a ys D2081 P2086 27 (SEQ ID NO: 951) Z ea m a ys D2081 P2086 114 WO 2024 / 206966 PCT / US2024 / 022430 [000116] The term “ACCase homolog” or any variation therefore refers to an ACCase gene or ACCase gene product found in the same or another species that performs the same or substantially the same biological function as the blackgrass (A lo p ecu ru s m yo su ro id es) ACCase genes and gene products disclosed herein and where the nucleic acid sequences or polypeptide sequences (of the ACCase gene product) are said to be “identical” or at least 50% similar (also referred to as “percent identity” or “substantially identical”) as described herein. Methods of Identifying Sequence Similarity [000117] Two polynucleotides or polypeptides are identical if the sequence of nucleotides or amino acid residues, respectively, in the two sequences is the same when aligned for maximum correspondence as described below. The terms “identical” or “percent identity,” in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence over a comparison window, as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. For polypeptides where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Means for making this adjustment are well known to those of skill in the art. Typically, this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated according to, e .g ., the algorithm of Meyers & Miller, Computer Applic. Biol. Sci. 4: 11-17 (1988) e.g ., as implemented in the program PC / GENE (Intelligenetics, Mountain View, Calif., U.S.A.), [000118] The phrases “substantially identical,” and “percent identity” in the context of two nucleic acids or polypeptides, refer to sequences or subsequences that have at least 50%, advantageously 60%, preferably 70%, more preferably 80%, and most preferably 90- 95% nucleotide or amino acid residue identity when aligned for maximum correspondence over a comparison window as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. This definition also refers to the 115 WO 2024 / 206966 PCT / US2024 / 022430 complement of a test sequence, which has substantial sequence or subsequence complementarity when the test sequence has substantial identity to a reference sequence. [000119] One of skill in the art will recognize that two polypeptides can also be “substantially identical” if the two polypeptides are immunologically similar. Thus, overall protein structure may be similar while the primary structure of the two polypeptides displays significant variation. Therefore, a method to measure whether two polypeptides are substantially identical involves measuring the binding of monoclonal or polyclonal antibodies to each polypeptide. Two polypeptides are substantially identical if the antibodies specific for a first polypeptide bind to a second polypeptide with an affinity of at least one third of the affinity for the first polypeptide. For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. [000120] Optimal alignment of sequences for comparison can be conducted, e .g ., by the local homology algorithm of Smith & Waterman, 0.4dv. Appl. Math. 2:482 (I 98 I), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Nat'l. Acad. Sci. U.S.A. 5 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), by software for alignments such as VECTOR NTI Version #11.5 by Life Technologies, Carlsbad, CA, U.S.A., by the procedures described in ClustalW, Thompson, J. D., Higgins, D. G. and Gibson, T. J. (1994) CLTiSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position—specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680 or by visual inspection (see generally, Protocols in Molecular Biology, F. M. Ausubel et al., eds., Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (1995 Supplement) (Ausubel)). [000121] Examples of algorithms that are suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al. (1990) J. Mol. Biol. 215: 403-410 and Altschul et al. (1977) Nucleic Acids Res. 25: 33 89-3402, respectively. Software for performing BLAST 116 WO 2024 / 206966 PCT / US2024 / 022430 analyses is publicly available through the National Center for Biotechnology Information (http: / / www.ncbi.nlm.nih.gov / ). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always>0) and N (penalty score for mismatching residues; always<0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a word length (W) of 11, an expectation (E) of 10, M=5, N=-4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a word length (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff, Proc. Natl. Acad. Sci. U.S.A. 89:10915 (1989)). In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e .g ., Karlin & Altschul, Proc. Nat'l. Acad. Sci. U.S.A. 90:5873-5787 (1993)). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.1, more preferably less than about 0.01, and most preferably less than about 0.001. Nucleic Acids and Delivery Thereof to Cells [000122] Certain aspects of the present disclosure involve nucleic acids (e.g ., ACCase genes), as well as nucleic acids having one or more mutations. Various methods exist for 117 WO 2024 / 206966 PCT / US2024 / 022430 inducing mutations in a nucleic acid, as described herein. In some embodiments, one or more nucleic acids may be delivered to a cell, as described herein. O lig o n u c leo b a ses [000123] As used herein, an “oligonucleobase” is a polymer of nucleobases, which polymer can hybridize by Watson-Crick base pairing to a DNA having the complementary sequence. [000124] Nucleobases comprise a base, which may be a purine, pyrimidine, or a derivative or analog thereof. Nucleobases include peptide nucleobases, the subunits of peptide nucleic acids, and morpholine nucleobases as well as nucleosides and nucleotides. Nucleosides are nucleobases that contain a pentosefuranosyl moiety, e .g ., an optionally substituted riboside or 2’-deoxyriboside. Nucleosides can be linked by one of several linkage moieties, which may or may not contain phosphorus. Nucleosides that are linked by unsubstituted phosphodiester linkages are termed nucleotides. [000125] An oligonucleobase chain may have a single 5’ and 3’ terminus, which are the ultimate nucleobases of the polymer. A particular oligonucleobase chain can contain nucleobases of all types. An oligonucleobase compound is a compound comprising one or more oligonucleobase chains that are complementary and hybridized by Watson-Crick base pairing. Nucleobases are either deoxyribo-type or ribo-type. Ribo-type nucleobases are pentosefuranosyl containing nucleobases wherein the T carbon is a methylene substituted with a hydroxyl, alkyloxy or halogen. Deoxyrib—type nucleobases are nucleobases other than ribo-type nucleobases and include all nucleobases that do not contain a pentosefuranosyl moiety. [000126] An oligonucleobase strand generically includes both oligonucleobase chains and segments or regions of oligonucleobase chains. An oligonucleobase strand has a 3’ end and a 5’ end. When an oligonucleobase strand is coextensive with a chain, the 3’ and 5’ ends of the strand are also 3 ’ and 5’ termini of the chain. [000127] The oligonucleobase can be introduced into a plant cell using any method commonly used in the art, including but not limited to, microcarriers (biolistic delivery), microfibers (whiskers), electroporation, nucleofection, PEG-mediated delivery, direct DNA uptake and microinjection. Illustrative examples of an oligonucleobase are described below. 118 WO 2024 / 206966 PCT / US2024 / 022430 [000128] The description can be practiced with oligonucleobases having the conformations and chemistries described in the Kmiec I and Kmiec II patents which are incorporated herein by reference. Kmiec I discloses a method for introducing specific genetic alterations into a target gene. The oligonucleobases in Kmiec I and / or Kmiec II contain two complementary strands, one of which contains at least one segment of RNA- type nucleotides (an “RNA segment”) that are base paired to DNA-type nucleotides of the other strand. [000129] Kmiec II discloses that purine and pyrimidine base-containing non­ nucleotides can be substituted for nucleotides. U.S. Patent Nos. 5,756,325; 5,871,984; 5,760,012; 5,888,983; 5,795,972; 5,780,296; 5,945,339; 6,004,804; and 6,010,907 and in International Patent No. PCT / US00 / 23457; and in International Patent Publication Nos. WO 98 / 49350; WO 99 / 07865; WO 99 / 58723; WO 99 / 58702; WO 99 / 40789; U.S. Patent No. 6,870,075; and U.S. Published Patent Application 20030084473, which are each hereby incorporated in their entirety, disclose additional molecules that can be used for the present description. The term “oligonucleobase” is used herein to denote the molecules that can be used in the methods of the present disclosure and include mixed duplex oligonucleotides, non-nucleotide containing molecules taught in Kmiec II, single stranded oligodeoxynucleotides and other molecules taught in the above noted patents and patent publications. [000130] In one embodiment, the oligonucleobase is a mixed duplex oligonucleotide in which the RNA-type nucleotides of the mixed duplex oligonucleotide are made RNase resistant by replacing the 2'-hydroxyl with a fluoro, chloro or bromo functionality or by placing a substituent on the 2'-0. Suitable substituents include the substituents taught by the Kmiec II. Alternative substituents include the substituents taught by U.S. Pat. No. 5,334,711 (Sproat) and the substituents taught by patent publications EP 629 387 and EP 679 657 (collectively, the Martin Applications), which are incorporated herein by reference. As used herein, a 2'-fluoro, chloro or bromo derivative of a ribonucleotide or a ribonucleotide having a 2'-OH substituted with a substituent described in the Martin Applications or Sproat is termed a “2'-substituted ribonucleotide.” As used herein the term “RNA-type nucleotide” means a 2'-hydroxyl or 2'-substituted nucleotide that is linked to other nucleotides of a mixed duplex oligonucleotide by an unsubstituted phosphodiester linkage or any of the non-natural linkages taught by Kmiec I or Kmiec II. As used herein the term “deoxyribo-type nucleotide” means a nucleotide having a 2'-H, which can be 119 WO 2024 / 206966 PCT / US2024 / 022430 linked to other nucleotides of a MDON by an unsubstituted phosphodiester linkage or any of the non-natural linkages taught by Kmiec I or Kmiec II. [000131] In one embodiment of the present disclosure, the oligonucleobase or GRON is a mixed duplex oligonucleotide that is linked solely by unsubstituted phosphodiester bonds. In alternative embodiments, the linkage is by substituted phosphodiesters, phosphodiester derivatives and non-phosphorus-based linkages as taught by Kmiec II. In yet another embodiment, each RNA-type nucleotide in the mixed duplex oligonucleotide is a 2'- substituted nucleotide. Particularly preferred embodiments of 2'-substituted ribonucleotides are 2'-fluoro, 2'-methoxy, 2'-propyloxy, 2'-allyloxy, 2'-hydroxylethyloxy, 2'-methoxyethyloxy, 2'-fluoropropyloxy and 2'-trifluoropropyloxy substituted ribonucleotides. More preferred embodiments of 2'-substituted ribonucleotides are 2'- fluoro, 2'-methoxy, 2'-methoxyethyloxy, and 2'-allyloxy substituted nucleotides. In another embodiment the mixed duplex oligonucleotide is linked by unsubstituted phosphodiester bonds. [000132] Although mixed duplex oligonucleotides having only a single type of 2'- substituted RNA-type nucleotide are more conveniently synthesized, the methods of the disclosure can be practiced with mixed duplex oligonucleotides having two or more types of RNA-type nucleotides. The function of an RNA segment may not be affected by an interruption caused by the introduction of a deoxynucleotide between two RNA-type trinucleotides, accordingly, the term RNA segment encompasses such an “interrupted RNA segment.” An uninterrupted RNA segment is termed a contiguous RNA segment. In an alternative embodiment an RNA segment can contain alternating RNase-resistant and unsubstituted 2'-OH nucleotides. The mixed duplex oligonucleotides preferably have fewer than 100 nucleotides and more preferably fewer than 85 nucleotides, but more than 50 nucleotides. The first and second strands are Watson-Crick base paired. In one embodiment the strands of the mixed duplex oligonucleotide are covalently bonded by a linker, such as a single stranded hexa, penta or tetranucleotide so that the first and second strands are segments of a single oligonucleotide chain having a single 3' and a single 5' end. The 3' and 5' ends can be protected by the addition of a “hairpin cap” whereby the 3' and 5' terminal nucleotides are Watson-Crick paired to adjacent nucleotides. A second hairpin cap can, additionally, be placed at the junction between the first and second strands distant from the 3' and 5' ends, so that the Watson-Crick pairing between the first and second strands is stabilized. 120 WO 2024 / 206966 PCT / US2024 / 022430 [000133] The first and second strands contain two regions that are homologous with two fragments of the target ACCase gene, i.e ., have the same sequence as the target gene. A homologous region contains the nucleotides of an RNA segment and may contain one or more DNA-type nucleotides of connecting DNA segment and may also contain DNA-type nucleotides that are not within the intervening DNA segment. The two regions of homology are separated by, and each is adjacent to, a region having a sequence that differs from the sequence of the target gene, termed a “heterologous region.” The heterologous region can contain one, two or three mismatched nucleotides. The mismatched nucleotides can be contiguous or alternatively can be separated by one or two nucleotides that are homologous with the target gene. Alternatively, the heterologous region can also contain an insertion or one, two, three or of five or fewer nucleotides. Alternatively, the sequence of the mixed duplex oligonucleotide may differ from the sequence of the target gene only by the deletion of one, two, three, or five or fewer nucleotides from the mixed duplex oligonucleotide. The length and position of the heterologous region is, in this case, deemed to be the length of the deletion, even though no nucleotides of the mixed duplex oligonucleotide are within the heterologous region. The distance between the fragments of the target gene that are complementary to the two homologous regions is identically the length of the heterologous region when a substitution or substitutions is intended. When the heterologous region contains an insertion, the homologous regions are thereby separated in the mixed duplex oligonucleotide farther than their complementary homologous fragments are in the gene, and the converse is applicable when the heterologous region encodes a deletion. [000134] The RNA segments of the mixed duplex oligonucleotides are each a part of a homologous region, i.e., a region that is identical in sequence to a fragment of the target gene, which segments together preferably contain at least 13 RNA-type nucleotides and preferably from 16 to 25 RNA-type nucleotides or yet more preferably 18-22 RNA-type nucleotides or most preferably 20 nucleotides. In one embodiment, RNA segments of the homology regions are separated by and adjacent to, i.e., “connected by” an intervening DNA segment. In one embodiment, each nucleotide of the heterologous region is a nucleotide of the intervening DNA segment. An intervening DNA segment that contains the heterologous region of a mixed duplex oligonucleotide is termed a “mutator segment.” [000135] The change to be introduced into the target gene is encoded by the heterologous region. The change to be introduced into the ACCase gene may be a change 121 WO 2024 / 206966 PCT / US2024 / 022430 in one or more bases of the target gene sequence that changes the native amino acid in that position to the desired amino acid. [000136] In another embodiment of the present disclosure, the oligonucleobase is a single stranded oligodeoxynucleotide mutational vector or SSOMV, which is disclosed in International Patent Application PCT / US00 / 23457, which is incorporated herein by reference in its entirety. The sequence of the SSOMV is based on the same principles as the mutational vectors described in U.S. Pat. Nos. 5,756,325; 5,871,984; 5,760,012; 5,888,983; 5,795,972; 5,780,296; 5,945,339; 6,004,804; and 6,010,907 and in International Publication Nos. WO 98 / 49350; WO 99 / 07865; WO 99 / 58723; WO 99 / 58702; WO 99 / 40789; U.S. 6,870,075; and U.S. Published Patent Application 20030084473. The sequence of the SSOMV contains two regions that are homologous with the target sequence separated by a region that contains the desired genetic alteration termed the mutator region. The mutator region can have a sequence that is the same length as the sequence that separates the homologous regions in the target sequence but having a different sequence. Such a mutator region will cause a substitution. [000137] The nucleotides of the SSOMV are deoxyribonucleotides that are linked by unmodified phosphodiester bonds except that the 3' terminal and / or 5' terminal internucleotide linkage or alternatively the two 3' terminal and / or 5' terminal internucleotide linkages can be a phosphorothioate or phosphoamidate. As used herein an internucleotide linkage is the linkage between nucleotides of the SSOMV and does not include the linkage between the 3' end nucleotide or 5' end nucleotide and a blocking substituent, see supra. In a specific embodiment the length of the SSOMV is between 21 and 55 deoxynucleotides and the lengths of the homology regions are, accordingly, a total length of at least 20 deoxynucleotides and at least two homology regions should each have lengths of at least 8 deoxynucleotides. [000138] The SSOMV can be designed to be complementary to either the coding or the non-coding strand of the target gene. When the desired mutation is a substitution of a single base, it is preferred that both mutator nucleotides be a pyrimidine. To the extent that is consistent with achieving the desired functional result it is preferred that both the mutator nucleotide and the targeted nucleotide in the complementary strand be pyrimidines. Particularly preferred are SSOMV that encode transversion mutations, i.e., a C or T mutator nucleotide is mismatched, respectively, with a C or T nucleotide in the complementary strand. 122 WO 2024 / 206966 PCT / US2024 / 022430 [000139] In addition to the oligodeoxynucleotide the SSOMV can contain a 5' blocking substituent that is attached to the 5' terminal carbons through a linker. The chemistry of the linker is not critical other than its length, which should preferably be at least 6 atoms long and that the linker should be flexible. A variety of non-toxic substituents such as biotin, cholesterol or other steroids or a non-intercalating cationic fluorescent dye can be used. Particularly preferred as reagents to make SSOMV are the reagents sold as Cy3™ and Cy5™ by Glen Research, Sterling VA (acquired by Maravai LifeSciences), which are blocked phosphoroamidites that upon incorporation into an oligonucleotide yield 3,3,3',3'- tetramethyl N,N'-isopropyl substituted indomonocarbocyanine and indodicarbocyanine dyes, respe...

Claims

Attorney Docket: CIBUS-045-PCT CLAIMS We claim:

1. A plant or plant cell having two or more mutations in an ACCase gene such that the mutated gene encodes an ACCase protein comprising a first amino acid substitution at a position corresponding to position D2078 of SEQ ID NO: 2 and a second amino acid substitution at a position corresponding to position P2083 of SEQ ID NO:

2.

2. The plant or plant cell of claim 1, wherein the first substitution is selected from D2078G, D2078K, D2078T, and D2078V.

3. The plant or plant cell of claim 1 or 2, wherein the first substitution is D2078G.

4. The plant or plant cell of any of the preceding claims, wherein the second substitution is selected from P2083T, P2083S, P2083N, and P2083Q.

5. The plant or plant cell of any of the preceding claims, wherein the second substitution is P2083T.

6. The plant or plant cell of any of the preceding claims, wherein the first substitution is D2078G, and the second substitution is P2083T.

7. The plant or plant cell of any of the preceding claims, wherein the plant or plant cell belongs to the genus Oryza.

8. An Oryza plant or plant cell having two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising a first amino acid substitution at a position corresponding to position D2089 of SEQ ID NO: 4 and a second amino acid substitution at a position corresponding to position P2094 of SEQ ID NO: 4; or a first amino acid substitution at a position corresponding to position D2089 of SEQ ID NO: 954 and a second amino acid substitution at a position corresponding to position P2094 of SEQ ID NO:

954.

9. The Oryza plant or plant cell of claim 8, wherein the first substitution is D2089G.

10. The Oryza plant or plant cell of any of the preceding claims, wherein the second substitution is P2094T.

11. The Oryza plant or plant cell of any of the preceding claims, wherein the first substitution is D2089G, and the second substitution is P2094T.Attorney Docket: CIBUS-045-PCT 12. An Oryza plant or plant cell having two or more mutations in an ACCase gene such that the mutated gene encodes a protein comprising a first amino acid substitution at a position corresponding to position D2076 of SEQ ID NO: 6 and a second amino acid substitution at a position corresponding to position P2081 of SEQ ID NO:

6.

13. The Oryza plant or plant cell of claim 12, wherein the first substitution D2076G.

14. The Oryza plant or plant cell of any of the preceding claims, wherein the second substitution is P2081T.

15. The Oryza plant or plant cell of any of the preceding claims, wherein the first substitution is D2076G, and the second substitution is P2081T.

16. A plant or plant cell expressing an ACCase protein comprising a first amino acid substitution at a position corresponding to position D2078 of SEQ ID NO: 2 and a second amino acid substitution at a position corresponding to position P2083 of SEQ ID NO:

2.

17. The plant or plant cell of claim 16, wherein the first substitution is selected from D2078G, D2078K, D2078T, and D2078V.

18. The plant or plant cell of claim 16 or 17, wherein the first substitution is D2078G.

19. The plant or plant cell of one of claims 16-18, wherein the second substitution is selected from P2083T, P2083S, P2083N, and P2083Q.

20. The plant or plant cell of one of claims 16-19, wherein the second substitution is P2083T.

21. The plant or plant cell of one of claims 16-20, wherein the first substitution is D2078G, and the second substitution is P2083T.

22. The plant or plant cell of one of claims 16-21, wherein the plant or plant cell belongs to the genus Oryza.

23. An Oryza plant or plant cell expressing a protein comprising a first amino acid substitution at a position corresponding to position D2089 of SEQ ID NO: 4 and a second amino acid substitution at a position corresponding to position P2094 of SEQ ID NO: 4; or a first amino acid substitution at a position corresponding to position D2089 of SEQ ID NO: 954 and a second amino acid substitution at a position corresponding to position P2094 of SEQ ID NO:

954.

24. The Oryza plant or plant cell of claim 23, wherein the first substitution is D2089G.

25. The Oryza plant or plant cell of one of claims 23 or 24, wherein the second substitution is P2094T.Attorney Docket: CIBUS-045-PCT 26. The Oryza plant or plant cell of one of claims 23-25, wherein the first substitution is D2089G, and the second substitution is P2094T.

27. An Oryza plant or plant cell expressing a protein comprising a first amino acid substitution at a position corresponding to position D2076 of SEQ ID NO: 6 and a second amino acid substitution at a position corresponding to position P2081 of SEQ ID NO:

6.

28. The Oryza plant or plant cell of claim 27, wherein the first substitution D2076G.

29. The Oryza plant or plant cell of one of claims 27 or 28, wherein the second substitution is P2081T.

30. The Oryza plant or plant cell of one of claims 27-29, wherein the first substitution is D2076G, and the second substitution is P2081T.

31. The plant or plant cell of any of the preceding claims wherein such plant or plant cell is resistant to one or more of alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, profoxydim, sethoxydim, tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, diclofop-methyl, fenoxaprop, fenoxaprop-P-ethyl, fenthiaprop, fluazifop, fluazifop-P-butyl, haloxyfop, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P-ethyl, trifop, pinoxaden, or an agronomically acceptable salts or esters thereof.

32. The plant or plant cell of any of the preceding claims wherein such plant or plant cell is resistant to clethodim.

33. The plant or plant cell of any of the preceding claims wherein such plant or plant cell is fertile.

34. The plant or plant cell of any of the preceding claims wherein such plant or plant cell nontransgenic with respect to the ACCase protein.

35. The plant or plant cell of any of the preceding claims wherein the plant species is selected from the group consisting of canola, sunflower, corn, tobacco, sugar beet, cotton, wheat (including but not limited to Triticum spp., Triticum aestivum, Triticum durum Triticum timopheevii, Triticum monococcum, Triticum spelta, Triticum zhukovskyi and Triticum urartu and hybrids thereof), barley (including but not limited to Hordeum vulgare L., Hordeum comosum, Hordeum depressum, Hordeum intercedens, Hordeum jubatum, Hordeum marinum, Hordeum marinum, Hordeum parodii, Hordeum pusillum, Hordeum secalinum, and Hordeum spontaneum), rice (including but not limited to Oryza sativa subsp. indica, Oryza sativa subsp. japonica, Oryza sativa subsp. javanica, Oryza sativa subsp. glutinosaAttorney Docket: CIBUS-045-PCT (glutinous rice), Oryza sativa Aromatica group (e.g., basmati), and Oryza sativa (floating rice group)), alfalfa, sorghum (e.g., Sorghum bicolor), foxtail millet (e.g., Setaria italica), switchgrass (e.g., Panicum virgatum), Kentucky blue-grass (e.g., Poa pratensis), tomato, mango, peach, apple, pear, strawberry, banana, melon, cassava, potato, carrot, lettuce, onion, soy bean, soya spp, sugar cane, pea, chickpea, field pea, fava bean, lentils, turnip, rutabaga, brussels sprouts, lupin, cauliflower, kale, field beans, poplar, pine, eucalyptus, grape, citrus, triticale, alfalfa, rye (including but not limited to Secale sylvestre, Secale strictum, Secale cereale, Secale vavilovii, Secale africanum, Secale ciliatoglume, Secale ancestrale, and Secale montanum), perennial ryegrass (e.g., Lolium perenne), oats (e.g., Avena sativa), turf (including but not limited to Turf grass include Zoysia japonica, Agrostris palustris, Poa pratensis, Poa annua, Digitaria sanguinalis, Cyperus rotundus, Kyllinga brevifolia, Cyperus amuricus, Erigeron canadensis, Hydrocotyle sibthorpioides, Kummerowia striata, Euphorbia humifusa, and Viola arvensis) and forage grasses, flax, oilseed rape, cotton, mustard, cucumber, morning glory, balsam, pepper, eggplant, marigold, lotus, cabbage, daisy, carnation, tulip, iris, and lily.

36. A method of making a plant or plant cell of any of the preceding claims comprising modifying an ACCase gene of a plant or plant cell.

37. A method of making a plant or plant cell of any of the preceding claims, said method comprising exposing a plant cell to a DNA cutter and a modified GRON configured to introduce one or more base pair mismatches with a genomic target site.

38. A method according to claim 37, wherein the DNA cutter is site-specific and is configured to introduce a double strand break or a single strand nick within 2kb of the genomic target site.

39. A method for making a plant or plant cell of any of the preceding claims comprising: a) introducing into plant cells a gene repair oligonucleobase configured to introduce one or more a targeted mutations in the ACCase gene to produce plant cells with a mutant ACCase gene that expresses an ACCase protein that is substituted at one or more amino acid positions, b) selecting a plant cell exhibiting improved tolerance to one or more of alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, sethoxydim,Attorney Docket: CIBUS-045-PCT tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, diclofop, fenoxaprop, fenoxaprop-P, fenthiaprop, fluazifop, fluazifop-P, haloxyfop, haloxyfop-P, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P, trifop, pinoxaden, agronomically acceptable salts and esters of any of these herbicides, and combinations thereof as compared to a corresponding wild-type plant cell; and c) regenerating a non-transgenic herbicide resistant plant having a mutated ACCase gene from said selected plant cell.

40. A method for making a plant or plant cell of any of the preceding claims comprising: a) introducing into plant cells a gene repair oligonucleobase with a targeted mutation in the ACCase gene to produce plant cells with a mutant ACCase gene that expresses an ACCase protein that is substituted at one or more amino acid positions, b) selecting a plant cell exhibiting improved tolerance to one or more of alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tepraloxydim, tralkoxydim, chlorazifop, clodinafop, clofop, diclofop, fenoxaprop, fenoxaprop-P, fenthiaprop, fluazifop, fluazifop-P, haloxyfop, haloxyfop-P, isoxapyrifop, propaquizafop, quizalofop, quizalofop-P, trifop, pinoxaden, agronomically acceptable salts and esters of any of these herbicides, and combinations thereof as compared to a corresponding wild-type plant cell; and c) regenerating a non-transgenic herbicide tolerant plant having a mutated ACCase gene from said selected plant cell.

41. A method according to claim 39 or 40, further comprising introducing into the plant cells a DNA cutter, wherein the DNA cutter is site-specific and is configured to introduce a double strand break or a single strand nick within 2kb of the targeted mutation.

42. A method according to claim 41, further compising culturing and regeneration methods to obtain a plant that produces seeds, and the production of seeds and additional plants including descendant (progeny) plants that contain the mutated ACCase gene.

43. The method of any of the preceeding embodiments, wherein said plant is fertile.

44. A plant ACCase protein comprising a first amino acid substitution at a position corresponding to position D2078 of SEQ ID NO: 2 and a second amino acid substitution at a position corresponding to position P2083 of SEQ ID NO: 2.Attorney Docket: CIBUS-045-PCT 45. The plant ACCase protein of claim 44, wherein the first substitution is selected from D2078G, D2078K, D2078T, and D2078V.

46. The plant ACCase protein of claim 44 or 45, wherein the first substitution is D2078G.

47. The plant ACCase protein of one of claims 44-46, wherein the second substitution is selected from P2083T, P2083S, P2083N, and P2083Q.

48. The plant ACCase protein of one of claims 44-47, wherein the second substitution is P2083T.

49. The plant ACCase protein of one of claims 44-47, wherein the first substitution is D2078G and the second substitution is P2083T.

50. The plant ACCase protein of one of claims 44-49, wherein the plant ACCase protein belongs to the genus Oryza.

51. An Oryza ACCase protein comprising a first amino acid substitution at a position corresponding to position D2089 of SEQ ID NO: 4 and a second amino acid substitution at a position corresponding to position P2094 of SEQ ID NO: 4; or a first amino acid substitution at a position corresponding to position D2089 of SEQ ID NO: 954 and a second amino acid substitution at a position corresponding to position P2094 of SEQ ID NO:

954.

52. The Oryza ACCase protein of claim 48, wherein the first substitution is D2089G.

53. The Oryza ACCase protein of one of claims 48 or 49, wherein the second substitution is P2094T.

54. The Oryza ACCase protein of one of claims 48-50, wherein the first substitution is D2089G, and the second substitution is P2094T.

55. An Oryza ACCase proteinexpressing a protein comprising a first amino acid substitution at a position corresponding to position D2076 of SEQ ID NO: 6 and a second amino acid substitution at a position corresponding to position P2081 of SEQ ID NO:

6.

56. The Oryza ACCase protein of claim 52, wherein the first substitution D2076G.

57. The Oryza ACCase protein of one of claims 52-53, wherein the second substitution is P2081T.

58. The Oryza ACCase protein of one of claims 52-54, wherein the first substitution is D2076G, and the second substitution is P2081T.