METHOD FOR MAKING CINNAMON (Cinnamomum burmannii) BARK EXTRACT GEL AS A WOUND HEALING AGENT IN THE ORAL MUCOSA

Abstract

This invention concerns a method for making cinnamon bark (Cinnamomum burmannii) extract gel as a wound healing agent in the oral mucosa. The preparation of cinnamon bark (Cinnamomum burmannii) extract gel is carried out in two stages, namely: the extraction stage and the gel preparation stage. First, cinnamon bark (Cinnamomum burmannii) is extracted using the maceration method with 96% ethanol, with a ratio of fine powder weight to ethanol volume of 1:10. The mixture is stirred for 3 days at room temperature at a speed of 600 rpm, then filtered to obtain the filtrate. The filtrate is dissolved again with 96% ethanol in a ratio of filtrate weight to ethanol volume of 1:5 and stirred for 2 days. After filtering, the filtrate is heated at 60°C until the ethanol evaporates, leaving a concentrated extract. In the gel preparation stage, CMC-Na powder is dissolved in 100 ml of distilled water that has been heated at 80-90°C until homogeneous.Then, 30 grams of concentrated cinnamon (Cinnamomum burmanii) extract was mixed with the CMC-Na solution. Next, 0.2 grams of methylparaben was added to 10 grams of glycerin and 5 grams of propylene glycol, then mixed with the extract. This mixture was stirred until homogeneous, forming a gel mass.

Description

METHOD FOR MAKING CINNAMON BARK EXTRACT GEL (Cinnamomum burmannii) AS A WOUND HEALING AGENT IN ORAL MUCOSA Invention Engineering Field The present invention relates to a method of preparing a gel cinnamon bark extract (Cinnamomum burmannii) as wound healing agent in the oral mucosa. Background of the Invention Oral mucosa is the tissue lining the oral cavity. functions as a “gateway” to the body and becoming first line protection against potential pathogens, materials exogenous chemicals, as well as airborne allergens. The mucosa consists of cells epithelial cells that act as a barrier by forming layers continuously and protects the body through its physical and immunological barrier function (Senel, 2021j doi: 10.3390 / ijms22157821). Wounds occur due to changes continuity of skin or mucosal tissue both cellularly and anatomy. When tissue is damaged, the healing process wound healing will occur (Nguyen akk., 2023j DOI: 10.1039 / D2RA07673J). In recent decades, herbal medicines have begun to its use as an alternative treatment option is being explored. One of the spice commodities that is still traded well in both regional and international markets are cinnamon (Cinnamomum burmannii). Several studies have proven that Cinnamomum burmanii has benefits including as antioxidant, antiulcer, antibacterial, antidiabetic, and anti-inflammatory (Emilda, 2018: DOI: https: / / doi.org / 10.33096 / jffi.v5i1.316). Invention regarding Cinnamomum Burmannii cinnamon stick extract has been widely conducted. Research on cinnamon oleoresin extraction (Cinnemomum burmanii) has been reported in a technology journal Agricultural products vol VI. No. 1 year 2013. Wood extraction process sweet using maceration method to determine yield optimum in the cinnamon oleoresin production process (Widiyanto et al., 2013). However, this research still contains the disadvantage is that the filtration process for the filtrate and residue only was conducted once. In addition, the research was only shows the method of making cinnamon bark extract (Cinnemomum Burmannii) only. Not yet ready stock use on humans. Invention in the form of cinnamon leaf extract gel preparation (Cinnemomum Burmannii) has been reported in Makassar Dental Journal volume 12 No. 2 of 2013, shows that the leaves Cinnamon concentration 358 effectively increases healing buccal mucosal wounds of rats through a review of the number of fibroblast cells. However, this invention has a drawback, namely the process long drying of cinnamon leaves (Cinnamomum burmannii) that is, one month in a room that is not exposed to sunlight direct sunlight. This invention uses the leaf part of the cinnamon (Cinnamomum burmannii). Cinnamon stem extract gel sweet for healing wounds in the oral mucosa yet ever existed in previous research reports. Prior invention with patent number IDP000094027, related to the process of making hand sanitizer preparations which contains cinnamon extract (Cinnamomum burmannii). This invention process begins with the extraction of wood simplicia sweet (Cinnamomum burmannii) using ethanol solvent 968 with a ratio of cinnamon simples and solvent (1:4), followed by filtration and evaporation at a temperature of 60°C until a cinnamon extract is obtained in the form of a paste. Invention This is in the form of a hand sanitizer gel preparation containing cinnamon extract 208: carbopol 940 0.5 mg: TEA 0.2 mL: nipagin 0.2 mg, glycerin 1 mL: alcohol 5 ml and distilled water up to a total of 100 ml. The gel preparation invention has been proven to be capable of inhibits the growth of the total number of bacterial colonies on hand. However, this invention still has shortcomings, namely the manufacturing process only produces the preparations used limited as an antibacterial on the surface of the hands. Method for making cinnamon bark extract gel (Cinnamomum burmannii) can produce preparations in the form of gel used as an easy wound healing agent applied in the oral cavity in cases of damaged integrity Epithelial tissue and the tissue underneath by pushing formation of new blood vessels, reducing the diameter and increase the percentage of contraction of oral mucosal wounds thus accelerating the closure of mucosal wounds in the oral cavity. Furthermore, the proposed invention is intended to to overcome the problems stated above, namely provides a gel-shaped wound healing agent method easy to apply from cinnamon bark extract (Cinnemomum burmannii) for the oral mucosa. Brief Description of the Invention The main object of the present invention is to provide oral mucosa wound healing agent in gel form cinnamon bark extract (Cinnamomum burmannii) with how to encourage the formation of new blood vessels, reduce diameter and increase the percentage of mucosal wound contraction oral cavity thereby accelerating the closure of mucosal wounds in oral cavity. The present invention provides a method for preparing a gel cinnamon bark extract (Cinnamomum Burmannii) as wound healing agent in the oral mucosa. Gel preparation through the stages of making cinnamon bark gel extract (Cinnamomum burmannii) using the maceration method with ethanol solvent: filtration, maceration, re-filtration and heating until a thick extract is obtained. Complete Description of the Invention Gel-based wound healing agent for the mucosa of the oral cavity from cinnamon bark extract (Cinnamomum burmeannii) with a solid consistency, brown in color, has a distinctive aroma cinnamon, has good spreading and adhesive power so it is easy to apply to the injured area in the oral cavity. Cinnamon sticks (Cinnamomum burmannii) are used here refers to the cinnamon plant which comes from Jambi Province. Plant identification tests have been carried out on Phytochemistry Laboratory Unit 2, Faculty of Pharmacy, University Muhammadiyah Yogyakarta, Indonesia, with cinnamon products family lauraceae, species Cinnamomum burmannii. The process of making cinnamon extract gel (Cinnamomum burmannii) through two stages, namely the extraction stage and the gel making stage. The extraction making stage includes the following stages: a. prepare cinnamon bark (Cinnamomum burmannii): b. chopping cinnamon bark (Cinnamomum burmannii) as (a) with a length of 2-3 cm and a width of 1 cm cm to speed up the drying process: c. Wash the bark of cinnamon (Cinnamomum burmannii) as (b) twice with deionized waterj da. draining the bark of cinnamon (Cinnamomum burmannii) as (c): e. drying the bark of cinnamon (Cinnamomum burmannii) as (d) by using an oven on temperature of 60”C for 24 hours for more even drying and not affected by the weather: . soften the bark of cinnamon (Cinnamomum burmannii). has been dried as (e) using a cutter mill for 1 minute until it becomes a coarse powder, . sift coarse powder as (£) use sieve (mesh) with a size of 500 to 53 microns for get fine powder. . Mix the fine powder as in (g) using ethanol 968 with a powder to ethanol ratio of 1 10 (w : v) so that it becomes a fine powder solution: L. stir the fine powder solution as (h) using stirrer, at room temperature, for 3 x 24 hours with 600rpm speed: j. filter the fine powder solution as (1) using filter paper to produce a filtrate . . dissolve (macerate) the filtrate as (J7) using ethanol 968 with a ratio of filtrate and ethanol, namely 1:5 (W: vw): . stir the solution as (k) using a stirrer at a speed of 600 rpm, for 2 x 24 hours at a temperature room: . filter the solution as (1) using paper filter to produce filtrate: . heat the filtrate as (m) at a temperature of 60”C to evaporate the remaining ethanol until a concentrated extract is formed. The next stage in the method of making extract gel cinnamon bark (Cinnamomum Burmannii) includes the following stages: a. dissolve the CMC-Na powder with distilled water heated at a temperature of 80-90”C as much as 100 ml using mortar and pestle until homogeneous: . mix the concentrated extract as (n) as much as 30 grams with CMC-Na solution as (a):j c. adding 0.2 g of methyl paraben to 10 g of glycerin and 5 g of propylene glycol into the extract mixture as (b): and d. stir the mixture as (c) using a mortar and pestle until homogeneous and forms a gel mass. After making the bark extract gel sweet, several tests were carried out, one of which was the test Physical Quality. Oral mucosa wound healing agent in the form of gel preparation from cinnamon bark extract (Cinnamomum) Burmannii has the following physical quality tests: a) Test organoleptic test shows that the gel preparation has a solid texture, brown in color, and has a distinctive cinnamon smell: b) homogeneity test shows that the gel preparation looks homogeneous and there is no coarse particles, c) good spreadability and adhesion test, d) good pH indicates that the gel preparation has the appropriate pH. Quality test physical examination is carried out in accordance with Indonesian National Standards (SNI), with reference number SNI 16-4769-1998, presented in table 1. Table 1. Physical quality test of bark extract gel preparations sweet Cinnamomum Burmannii No. Test Criteria Test Results l. Organoleptic test Solid Texture Brown The distinctive smell of cinnamon 2. Homogeneity Test 3. Good spreadability test 4, Good adhesion test 5. pH test 5 In addition to physical quality testing, the extract gel preparation is also in vivo activity testing was carried out on mice (Rattus Norvegicus) alloxan-induced diabetes such as as shown in table 2. Table 2. Results of macroscopic and microscopic observations in vivo in mice after administration of bark extract gel cinnamon Cinnamomum Burmannii Time Wound Diameter Contraction Formation observation (cm?) of vascular wounds new blood Day 3 0.08 t 0.03 12.60 t 8.18 6.33 t 2.19 Day 7 0.08 t 0.04 14.99 £ 5.53 10.33 £ t 2.15 Day 14 0.05 t 0.01 23.28 t 5.01 8.21 £t 1.85 The conclusion from the test results above is the gel method cinnamon extract (Cinnamomum Burmannii) as an agent oral mucosal wound healing test results show optimal physical quality according to the gel preparation criteria good and the results of macroscopic physical quality testing and microscopically shows increased mucosal wound healing labial oral cavity is more quickly characterized by the presence of formation of new blood vessels, decrease in diameter and increased percentage of contraction of oral mucosal wounds compared to control.

Claims

1. A method for making bark extract gel sweet (Cinnamomum burmannii) as a healing agent wounds in the oral mucosa consisting of: e. prepare cinnamon bark (Cinnamomum burmannii): £. chop the bark of cinnamon (Cinnamomum burmannii) as (La) with the size 2-3 cm long and 1 cm wide to speed up drying process, g. wash the bark of cinnamon (Cinnamomum burmannii) as (1l.b) twice with deionized water, h. drain the bark of the cinnamon tree (Cinnamomum burmannii) as (1.c):

1. dry the bark of cinnamon (Cinnamomum burmannii) as in (1.d) by using oven at 60°C for 24 hours for drying more even and not affected by the weather, J7. Smoothing cinnamon bark (Cinnamomum burmannii) which has been dried as (1l.e) using a cutter mill for 1 minute until it becomes a coarse powder, k. sift the coarse powder as (1.£) using a sieve (mesh) with a size of 500 up to 53 microns to obtain fine powder.

1. Mix the fine powder as in (lg) using ethanol 968 with a powder ratio and ethanol, namely 1:10 (w:v) so that it becomes fine powder solution: . stir the fine powder solution as (lh) using a stirrer, at room temperature, for 3 x 24 hours at 600rpm: . filter the fine powder solution as (1.1) using filter paper to produce filtrate :j . dissolve (macerate) the filtrate as (1.yj) using ethanol 9658 with a ratio filtrate and ethanol, namely 1:5 (w : v):j . stir the solution as (1.k) using stirrer at a speed of 600 rpm, for 2 x 24 hours at room temperature: . filter the solution as (1.1) using filter paper to produce filtrate: . heat the filtrate as (1.m) at a temperature 60” Cc to evaporate the remaining ethanol until concentrated extract is formed: . dissolve the CMC-Na powder with distilled water has been heated at a temperature of 80-90”C for 100 ml using a mortar and pestle until homogeneous: . mix the concentrated extract as (1l.n) as much as 30 grams with CMC-Na solution as (1.o): . adding 0.2 g of methyl paraben to 10 g glycerin and 5 g of propylene glycol into the mixture extract as (1.p): and . stir the mixture as (1.g) using mortar and pestle until homogeneous and forms gel mass.