Methods of producing fc-containing proteins
IL328354A0Pending Publication Date: 2026-07-01ELI LILLY & CO
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Patent Information
- Application Number
- IL328354
- Authority / Receiving Office
- IL · IL
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2023-12-08
- Filing Date
- 2024-12-06
- Publication Date
- 2026-07-01
AI Technical Summary
Technical Problem
Current methods for producing recombinant Fc-containing proteins in cell cultures often result in issues such as protease clipping and unfavorable glycosylation, which can reduce protein yield and quality.
Method used
The method involves culturing mammalian cells expressing Fc-containing proteins in a bioreactor with a structured nutrient feeding schedule, including multiple nutrient feeds at specific time points during the culture period, to optimize protein production and minimize clipping and glycosylation issues.
Benefits of technology
This approach leads to efficient, high-titer production of Fc-containing proteins with improved quality profiles, reduced protease clipping, and favorable glycosylation patterns.
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Abstract
Provided herein are improved methods for producing an Fc-containing protein. The methods generally involve culturing a cell that expresses the Fc-containing protein in a production bioreactor for a first period of time followed by supplying a first nutrient feed and culturing the cell for a second period of time followed by supplying a second nutrient feed. The methods may further involve culturing the cell for a third period of time, followed by supplying a third nutrient feed.
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