Cho transcription control elements and uses thereof

IL328963APending Publication Date: 2026-08-01LONZA BIOLOGICS PLC
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Patent Information

Application Number
IL328963
Authority / Receiving Office
IL · IL
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-12-29
Filing Date
2024-12-23
Publication Date
2026-08-01

AI Technical Summary

Technical Problem

Existing recombinant gene transcription in CHO cells using cytomegalovirus (CMV) promoters is suboptimal due to lower activity in CHO cells, lifecycle issues, and limited control over expression ratios for complex multichain products, leading to unpredictable output.

Method used

Development of transcription control elements comprising a murine or human CMV enhancer sequence operably linked with mammalian endogenous promoters and optionally introns, featuring specific NFKB binding sites, to enhance and control gene expression in CHO cells.

Benefits of technology

The new transcription control elements provide higher and more stable recombinant protein expression, improved expression ratios for multichain products, and better long-term expression stability, reducing cellular toxicity and process variability.

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Abstract

The present disclosure provides novel transcription control elements comprising an enhancer element operably linked to a mammalian endogenous promoter sequence and optionally a mammalian intron sequence, resulting in strong fusion promoters for constitutive high-level recombinant gene transcription in mammalian cells including CHO cells or CHO-derived cells.
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