Herbal solution for growing flesh in wounds and healing wounds and cuts using the plant Avyar Salam

Abstract

One of the current problems is the growth and proliferation of damaged cells and ultimately the effective healing of wounds. In medical science, we are faced with various types of wounds that are difficult to heal. The treatment of chronic wounds usually takes longer than expected. In such a way that the healing phase may last more than 4 weeks or in some cases, healing may not occur after 4 weeks. Many of the drugs available for wound healing are also expensive. And wound healing takes a long time, which allows the absorption of various bacteria and fungi through the wound to other parts of the body, tissues and organs, especially vital organs, and this problem can lead to infection and untreatability or difficult treatment of the wound itself, and even endangering other parts of the body. Therefore, effective wound healing is one problem, and healing it in the shortest possible time to prevent infection is another. Fibroblast is an important cell that is involved in all stages of wound healing. Fibroblasts play a pivotal role in the processes of intercellular substance deposition and tissue remodeling. Fibroblasts appear both as builder cells that produce a collagen-rich matrix and as messenger cells that secrete growth factors that are important for communication between two cells during the healing process. The occurrence of interruptions in the wound healing process is a major clinical problem that imposes high costs on society. The main cells of the dermis are fibroblasts, which produce extracellular matrix glycoproteins, elastic and reticular fibers, glycosaminoglycans, and various collagens. As a result, the regeneration, growth, proliferation, and stimulation of fibroblast cells can be the main factor in the healing of various wounds such as chronic diabetic ulcers, burns, gastric and intestinal ulcers, and wounds in other organs of the body, bedsores, postoperative wounds, and skin wounds. This herbal solution has been made more efficient and has had superior results than other drugs in terms of accelerating the healing process and effective wound healing, as well as being cheaper. The herbal solution prepared in 4 different doses has been able to double the growth of fibroblast cells and has no toxic effect on these cells, and in an effective dose, it has increased the number of fibroblast cells by more than two times.

Description

Description of the invention Title of the invention Making a herbal solution from the plant Cyperus Rotundus L. to heal all types of wounds Technical background of the relevant invention The technical field of the invention is in the medical branch, section A, human needs, sub-section Health and Hygiene, and a herbal solution for growing flesh and repairing wounds and damaged cells, and ultimately treating all types of wounds. Inventor Asana Ahmadi Master's student in phytochemistry Technical problem and stating the objectives of the invention One of the current problems is the growth and proliferation of damaged cells and ultimately the effective healing of wounds. In medicine, we are faced with various types of wounds that are difficult to repair. Wounds are physical injuries that lead to opening or tearing of the skin and can cause physical and functional disorders. Skin wounds cause the loss of epithelial attachment or the loss of underlying connective tissue or without the loss of underlying connective tissue. Wound and tissue healing is a four-stage biological process planned, overlapping, dynamic and natural in the body, which includes homeostasis, inflammation, proliferation and regeneration. The treatment of chronic wounds usually takes longer than expected. So that the healing phase may last more than 4 weeks. Burn wounds are considered as a separate group. Because they differ from other skin wounds in their pathophysiology and in their natural course. Many of the drugs available for wound healing are also expensive.And wound healing takes a long time, which allows the absorption of various types of bacteria and fungi through the wound to other parts and areas of the body, tissues and organs, especially vital organs, and this problem can lead to infection and incurability or difficult treatment of the wound itself and even endangering other areas of the body. Therefore, effective wound healing is one problem and healing it in the shortest possible time to prevent infection is another problem, which must be considered in the manufacture of an effective drug to treat one or even both problems, as solving these two problems depends on the growth and stimulation of the cells involved in this matter. Therefore, making a herbal solution without side effects to repair damaged cells in all types of wounds, including skin wounds, diabetic wounds, stomach and intestinal ulcers, and other wounds such as burn wounds, etc., at a low and effective cost that is easily produced and made available to patients at a low price can solve the two problems mentioned in the treatment process and, on the other hand, eliminate the excessive burden of producing other herbal or chemical drugs and the side effects of chemical drugs such as causing skin inflammation. A description of the state of the prior art and the history of developments related to the claimed invention. Fibroblasts are important cells involved in all stages of wound healing. A large proportion of wound fibroblasts are myofibroblasts that appear in wound closure. Fibroblasts play a central role in the processes of extracellular matrix deposition and tissue remodeling. Fibroblasts appear both as builder cells that produce a collagen-rich matrix and as messenger cells that secrete growth factors that are important for communication between two cells during the healing process. The occurrence of interruptions in the wound healing process is a major clinical problem that imposes high costs on society. The papillary layer, which contains the main fibroblasts of the skin, produces an important factor required to initiate the process of epidermal proliferation. The main cells of the dermis are fibroblasts that produce extracellular matrix glycoproteins, elastic and reticular fibers, glycosaminoglycans, and various collagens. These cells are key proteins connecting the epidermis to the basal layer and are involved in the migration and proliferation of epidermal cells.Fibronectin, a key derivative of fibroblast cells, is a healing agent. Collagens are the most abundant proteins in animal tissues that play an important role in connective tissue homeostasis. Fibroblasts are the main cells that produce collagen and are responsible for its regeneration. Fibroblasts also secrete growth factors. As a result, the regeneration, growth, proliferation and stimulation of fibroblast cells can be the main factor in the healing of various wounds such as chronic diabetic ulcers, burns, gastric and intestinal ulcers and wounds in other organs of the body, bedsores, post-surgical wounds and skin wounds. The drugs developed so far have not succeeded in effective and rapid wound healing due to the problem of this problem in the medical community and the healing process is either not done properly or has been used for a long time, which has caused wound infection, and they are expensive, which has made it difficult for patients to afford them. This herbal solution has been more efficient and has had excellent results compared to other medications in terms of accelerating the wound healing process, effective wound healing, and being inexpensive.On the other hand, high-volume, low-cost production is very desirable for the manufacturer (pharmaceutical factories), which makes it easier for the consumer (patients suffering from various types of wounds) to obtain the medicine. On the other hand, among the minerals, calcium, sulfur, zinc, and boron are very effective in healing wounds. Providing a solution to an existing technical problem along with an accurate, sufficient, and integrated description of the invention Preparation of a herbal solution from the plant Cyperus Rotundus L. for rapid healing of chronic skin wounds such as diabetes, burns, stomach ulcers, intestinal ulcers, bedsores, post-surgical wounds and other wounds through rapid growth and proliferation of fibroblast cells without having a toxic effect on these cells and without complications. To prepare the herbal solution, the plant of Oyar Salam was dried and extracted and solubilized by maceration method. Then, the effect of different doses of the drug was examined in vitro on fibroblast cells, and the results of the MTT method were analyzed and evaluated by treating fibroblast cells. In this method, two factors were examined: first, that the solution did not have a toxic effect by not reducing the percentage of fibroblast cell viability compared to the control, and second, that the growth and proliferation of fibroblast cells was proven and measured by increasing the percentage of fibroblast cell viability compared to the control through growth-stimulating factors in the plant. According to the graph of all doses 1.5, 3, 5, 7, 10 mg / ml compared to the control, in which 100% of the cells are alive, in all doses the survival percentage is more than 100%, which indicates that the solution made is non-toxic to fibroblast cells, which is considered an advantage. On the other hand, survival percentages greater than 100 indicate growth, proliferation, and increase in fibroblast cells.The highest effect on fibroblast growth was observed at a dose of 5 mg / ml with a viability of 223.6552%, which is approximately 223%. This means that the prepared solution was able to more than double the number of fibroblast cells with its growth-stimulating factors. At doses of 3 mg / ml with a viability of 204.069% and 1.5 mg / ml with a viability of 200.6897%, after a dose of 5 mg / ml, it showed the property of doubling the number of fibroblast cells, respectively. At a dose of 7 mg / ml with a viability of 199.7931%, it showed a nearly doubling of fibroblast cells, and at a dose of 10 mg / ml with a viability of 137.931, it showed a nearly 1.3-fold increase in fibroblast cells. As can be seen in the graph, there was no toxic effect at any of the doses, and we witnessed the growth and proliferation of fibroblast cells at all of them.Therefore, this solution in three doses of 5, 3 and 1.5 mg / ml had a maximum effect in increasing the number of fibroblast cells and by doubling the number of fibroblast cells, it showed the effect of rapid wound healing with the growth of these cells and then an increase in collagen, fibronectin, extracellular matrix glycoproteins, elastic and reticular fibers and the secretion of growth factors by them. Therefore, this solution is considered an excellent therapeutic option for effective and rapid wound healing compared to other existing drugs in the healing of all types of wounds through its mechanism of action on the growth and proliferation of fibroblast cells. Calcium, sulfur, boron and zinc were considered in an effective amount and in sufficient doses in this solution. Explanation of shapes, maps and diagrams The vertical axis (Y-axis) shows the percentage of fibroblast cell viability and in fact the growth and proliferation of these cells at different doses and the horizontal axis (x-axis) shows the drug doses. In the photographs taken by microscope of the condition of the cells at different doses, it shows an increase in fibroblasts at doses of 1.5 to 10 and at doses of 3 and 5 mg / ml, a maximum increase compared to the control was observed. A clear and precise statement of the advantages of the claimed invention over prior inventions. This invention uses a herbal solution that has no side effects on healthy cells in the body, and the herbal solution can be produced at low cost and easily on an industrial scale in pharmaceutical factories. Given the needs of society, its low-cost production is possible to a large extent. It is effective and usable for all types of wounds such as skin wounds, diabetic wounds, stomach and intestinal ulcers, burns, bedsores, surgical wounds and other wounds through the rapid growth and proliferation of fibroblast cells without having a toxic effect on these cells and without complications. Given the low cost of its production, we can make the medicine available to the public cheaply. Description of at least one implementation method for implementing the invention Herbal solutions can be easily produced on a laboratory and industrial scale with inexpensive solvents and other materials. The plant does not require cultivation, is self-sufficient, and is one of the plants available in large quantities, requiring no special costs to harvest. Explicit mention of the industrial application of the invention Its industrial application is in medical uses and in the pharmaceutical industry, which requires a shaker and industrial oven to produce.

Claims

Claims What is claimed: Claim 1) The invention is to prepare a herbal solution from the Avyar Salam plant for the rapid and effective treatment and repair of wounds, from superficial and skin wounds to chronic wounds such as diabetic wounds, stomach and intestinal ulcers, bedsores, burn wounds, post-surgical wounds and other wounds, and in general any type of wound with healing by ingrowth of flesh. Claim 2) The herbal solution was prepared using 100 grams of Oyar Salam plant powder by maceration at 25 degrees Celsius for 48 hours in a dark environment. Claim 3) To analyze the therapeutic properties of the herbal solution, the fibroblast cell line was used as the basic and main cells for wound healing. Claim 4) After culturing fibroblast cells, different doses of the herbal solution were prepared and its effect on the target cells was evaluated using the MTT method. Claim 5) After the MTT test, the analysis data was evaluated and the cell proliferation rate was analyzed according to the Viability Cell %, and the results were clearly reported in the description of the invention. Claim 6) In the diagram and microscopic photographs included in the description of the invention, the effect of different doses of the drug on fibroblast cells was examined and its non-toxicity was proven. It was also shown that in 4 doses, it caused a doubling of fibroblast cells, and in one dose, its effect was more than 2 times, which indicates the excellent result in the wound healing process due to the increase in the number of fibroblast cells. Claim 7) The mechanism of action of the medicinal solution is on the growth and proliferation of fibroblast cells and the increase in collagen, fibronectin, extracellular matrix glycoproteins, elastic and reticular fibers and the secretion of growth factors by them, and ultimately the growth of flesh and healing of wounds due to the large increase in fibroblast cells and the above. Claim 8) According to the results obtained and expressed in the description of the invention, this herbal solution has no toxic effects on target cells, which indicates the above advantages without side effects or minimal side effects. Claim 9) The prepared herbal solution can be produced on an industrial scale at low cost and in large quantities and made available to the consumer.

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