Topical skin preparations

Abstract

To provide a topical skin composition for suppressing or restoring lymphatic vessel dysfunction. [Solution] The present invention uses one or more of the following as active ingredients: perilla extract, rice leaf hydrolysate, lotus extract, peony extract, angelica extract, mandarin orange extract, rice extract or its hydrolysate or fermented rice product, yeast extract, black soybean hydrolysate, fermented soybean product, and eggplant extract.

Description

[Technical Field]

[0001] The present invention relates to an external skin preparation that improves the function of lymphatic vessels. [Background Art]

[0002] In recent years, the relationship between aging and the function of lymphatic endothelial cells has attracted attention. For example, lymphatic vessels are known to have the function of discharging cellular waste products, excess water, and inflammatory cells generated by various factors such as ultraviolet radiation, air pollutants, and stress. When this function decreases due to aging or other causes, inflammatory cells are thought to accumulate in skin cells, which as a result leads to rough skin and aging phenomena of the skin. For this reason, there is a demand for active ingredients that are applicable to the skin and have effects of improving rough skin and preventing aging caused by decreased lymphatic vessel function. Conventionally, lymphatic function improving agents applicable to the skin are known from, for example, Patent Documents 1 and 2, however, there is still a demand for ingredients with higher safety and effectiveness. [Prior Art Documents] [Patent Documents]

[0003] [Patent Document 1] Japanese Patent Laid-Open No. 2003-221314 [Patent Document 2] Japanese Patent Laid-Open No. 2008-189609 [Non-Patent Documents]

[0004] [Non-Patent Document 1] Jie Chen, J. Steven Alexander, A. Wayne Orr1, International Journal of Cell Biology, Volume 2012, Article ID 853703, 12 pagesdoi:10.1155 / 2012 / 853703 [Summary of the Invention] [Problems to be Solved by the Invention]

[0005] In view of the problems of the prior art described above, the present inventors have conducted diligent research and have newly discovered that one or more of the following have the effect of suppressing or restoring the decline in lymphatic vessel function: perilla extract, rice leaf hydrolysate, lotus extract, peony extract, angelica extract, mandarin orange extract, rice extract or its hydrolysate or fermented rice product, yeast extract, black soybean hydrolysate, and eggplant extract. [Means for solving the problem]

[0006] The present invention relates to a topical skin composition for suppressing or restoring lymphatic vessel dysfunction, comprising one or more of the following as active ingredients: perilla extract, rice leaf hydrolysate, lotus extract, peony extract, angelica extract, mandarin orange extract, rice extract or its hydrolysate or fermented rice product, yeast extract, black soybean hydrolysate, fermented soybean product, and eggplant extract. [Effects of the Invention]

[0007] The present invention provides a composition for improving lymphatic vessel function, comprising one or more of the following as active ingredients: perilla extract, rice leaf hydrolysate, lotus extract, peony extract, angelica extract, mandarin orange extract, rice extract or its hydrolysate or fermented rice product, yeast extract, black soybean hydrolysate, fermented soybean product, and eggplant extract, characterized in that when applied to the skin, it suppresses or restores the decline in lymphatic vessel function within skin cells. Accordingly, the present invention provides an active ingredient that, when applied to the skin, promotes the excretion of inflammatory cells, cellular waste products, and excess water by lymphatic vessels, and suppresses rough skin, aging phenomena, or edema caused by the accumulation of inflammatory cells. [Brief explanation of the drawing]

[0008] [Figure 1] This figure shows the effect of the active ingredient according to the present invention on promoting the synthesis of integrin α9. [Figure 2] This figure shows the secretion-promoting effect of the chemokine CCL21, an active ingredient according to the present invention. [Modes for carrying out the invention]

[0009] In this invention, "shiso" refers to perilla of the genus Perilla in the family Lamiaceae, and generally includes those scientifically known as Perilla frutescensvar. Crispa or Perilla frutescensvar. Britton. Examples include green perilla, crinkled perilla, red perilla, spotted perilla, firm perilla, crinkled green perilla, or their varieties, subspecies, or hybrids, but this invention is not limited to these. Any part of the plant can be used, such as the whole plant, leaves, flowers, stems, seeds, fruits, or roots, but the use of leaves or the whole plant including leaves is preferred. Furthermore, there are no particular limitations on the harvesting time or size of the perilla parts used, and any size and harvesting time can be used.

[0010] In this invention, "rice" refers to any rice plant belonging to the genus Oryza of the family Poaceae, specifically any variety belonging to Oryza sativa. Examples include Koshihikari, Sasanishiki, Nihonbare, Akitakomachi, Kinuhikari, and Hanaechizen, but the variety is not limited to these. In this invention, the part of the rice plant used is preferably the leaves or the whole plant including the leaves. Furthermore, it is preferable to use leaves before heading, and the leaves may be heat-treated before the extraction process. This suppresses denaturation and deterioration caused by active components (enzymes, etc.) contained in the rice leaves, and prevents a decrease in activity during storage of the harvested rice leaves.

[0011] In this invention, "lotus" refers to any plant belonging to the genus Nelumbo of the family Nymphaeaceae, and any species of this genus can be used. Examples include the lotus flower (Nelumbo nucifera Gaertner) and the American yellow lotus (Nelumbo Lutea Pers.). In this invention, it is preferable to use the lotus "flower" alone, but it is also possible to include other parts such as leaves, seeds, stamens, pistils, stems, rhizomes, and embryos in addition to the flower.

[0012] In this invention, "peony" refers to the peony (Paeonia japonica) of the Paeoniaceae family, genus Paeonia. The plant species is not particularly limited, and examples include Paeonia lactiflora, Paeonia japonica, Paeonia obovata, and Paeonia suffruticosa. Furthermore, any part of the plant, such as the whole plant, leaves, flowers, stems, seeds, fruits, or roots, can be used for extraction, but the use of the whole plant or roots is preferred.

[0013] In this invention, "Angelica" refers to Angelica acutiloba, a plant belonging to the genus Angelica in the family Apiaceae, and the plant species is not particularly limited. The roots, leaves, or the entire plant can be used as the extraction part.

[0014] Furthermore, in this invention, "Tachibana" refers to the tachibana orange (Citrus tachibana), a member of the genus Citrus in the family Rutaceae, and the plant species is not particularly limited. In addition, the extracting part can be the peel or the fruit (including the pulp, peel, seeds, etc.).

[0015] In this invention, "rice" is not particularly limited and can include brown rice, germinated brown rice, polished rice, processed rice, or colored rice (black rice, purple rice, red rice, etc.), as well as the white bran and / or red bran contained in the brown rice of brown rice, germinated brown rice, and colored rice. Any type of rice can be used, such as non-glutinous rice or glutinous rice. Examples of processed rice include anti-allergenic rice, low-protein rice (e.g., low-glycerin rice), and fortified rice (e.g., gamma-aminobutyric acid rice).

[0016] In this invention, "soybean" refers to Glycine max, a species of soybean belonging to the genus Glycine of the family Fabaceae, and in particular, any of black soybeans, white soybeans, red soybeans, and green soybeans may be used.

[0017] The "yeast" used in the present invention is yeast of the genus *Saccharomyces*. In particular, the yeast used in the present invention is preferably yeast collected, isolated, preserved and selected from plants or soil. In plants, for example, sugar-containing portions contain yeast, and in the present invention, it is particularly preferable to use yeast (*Saccharomyces cerevisiae*) present in flower nectar, tree sap and the like. Examples of flowers include rose, cherry blossom, camellia, lily and the like. These yeasts are available from, for example, NBRC [NBRC (Biological Resource Center, NITE)], the Microbe Division / Japan Collection of Microorganisms (JCM)-RIKEN, the Microbe Section of the National Agriculture and Food Research Organization Genebank Project (MAFF), ATCC (American Type Culture Collection), and the like.

[0018] In the present invention, "eggplant" refers to *Solanum melongena* of the genus *Solanum* in the family Solanaceae, and is not particularly limited to any cultivar. For example, long eggplant, round eggplant, mizunasu water eggplant (*Solanum Melongena*; Egg plant c.v. 'Mizu-nasu', including for example 7 native lines, Mizunasu Yawara, Waisen Mizunasu, Senshu Kinugawa Mizunasu), Kamo eggplant and the like can be used.

[0019] A method for preparing an extract using the above materials is described below. First, usable extraction solvents include: water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene, and chloroform; and the like. These solvents may be used alone or as a mixture of two or more. Among these extraction solvents, the use of water, or a mixed solvent of water and a lower alcohol, or water and a polyhydric alcohol is preferred in the present invention, also from the viewpoint that the resulting extract can be widely applied to external skin preparations.

[0020] In preparing the extract, there is no particular limitation on the pH of the extract, but it is preferably in the range of pH 2 to 8. For pH adjustment, for example, depending on the plant species, lactic acid, citric acid, phosphoric acid, tartaric acid, hydrochloric acid, acetic acid, succinic acid or salts thereof, potassium hydroxide, sodium hydroxide, sodium carbonate or sodium bicarbonate, etc. may be mentioned.

[0021] Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, in the case of an immersion method using water as the extraction solvent, the extraction temperature is generally in the range of 1 to 90°C, preferably 5°C to 80°C, and the extraction time is generally in the range of 0.5 to 7 days, preferably 1 to 24 hours.

[0022] The extract obtained under the above conditions may generally be used directly as a component of an external preparation for skin while adjusting the pH to 4 to 8, or may be used after adjusting to a desired concentration by vacuum concentration or the like.

[0023] Next, a method for hydrolyzing an extract of rice leaves will be described below. Examples of the hydrolysis treatment method include decomposition treatment methods using an acid, an alkali or an enzyme.

[0024] When performing hydrolysis treatment using an enzyme, at least one enzyme selected from proteases, amylolytic enzymes, pectinolytic enzymes, fibrinolytic enzymes and lipolytic enzymes can be used.

[0025] As the protease, for example, actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase, bromelain, and complex proteases derived from microorganisms (for example, Neurase [manufactured by Amano Enzyme Inc.]) can be used.

[0026] Examples of starch-degrading enzymes that can be used include α-amylase, β-amylase, glucoamylase, and β-galactosidase.

[0027] Examples of pectin-degrading enzymes that can be used include pectinase, pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase, and polygalacturonase.

[0028] Examples of fibrin-degrading enzymes that can be used include cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, carrageenanase, arginase, fucoidanase, inulase, xylanase, and ligninase.

[0029] Examples of lipid-degrading enzymes that can be used include lipase and phospholipase.

[0030] Next, when preparing hydrolysates from rice or soybean extracts, hydrolysis is performed before, after, or in parallel with the extraction. Hydrolysis can be performed using acids, alkalis, or enzymes, but hydrolysis using enzymes is preferred.

[0031] Enzymes used in hydrolysis include proteolytic enzymes and lipolytic enzymes. Depending on the plant species, these enzymes may be used individually or in combination.

[0032] Examples of proteolytic enzymes include actinases and other actinases, pepsins and other pepsins, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase, and aminopeptidase, and bromelain. Examples of lipolytic enzymes include lipase. When using proteolytic enzymes and lipolytic enzymes, any of the above enzymes may be used individually or in combination.

[0033] Enzymatic hydrolysis is carried out by adding one or more enzymes to the respective extract solutions of rice and soybeans mentioned above, and allowing the enzymatic reaction to occur under conditions near the optimal pH and temperature of the enzymes used. When using two or more enzymes in combination, depending on the characteristics of the enzymes used, the two or more enzymes may be reacted simultaneously, or they may be reacted sequentially with or without changing the reaction conditions.

[0034] After the hydrolysis treatment using the above enzymes is completed, the enzymes are deactivated by an appropriate method, such as heating the enzyme treatment solution to 80°C or higher, to obtain an enzyme-treated hydrolysis product solution.

[0035] Furthermore, in this invention, rice or soybeans may be fermented by microorganisms to prepare and use a fermented product. When fermentation is performed, the rice or black soybeans themselves may be used as the source of fermentation, or extracts obtained by extracting them with a solvent may be used. In addition, when using extracts, it is possible to perform fermentation while the rice or black soybeans are still included, without removing the plant material to be extracted by solid-liquid separation.

[0036] For the fermentation of rice or soybeans, it is preferable to use one or more of the following microorganisms: lactic acid bacteria, yeast, and koji mold. Examples of lactic acid bacteria include Lactobacillus species such as Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus delbrueckii; Carnobacterium species such as Carnobacterium divergens and Carnobacterium piscicola; and Leuconostoc mesenteroides, Leuconostoc lactis, and Leuconostoc citreum. Lactic acid bacteria of the genus Leuconostoc, such as *Leuconostoc citreum*; lactic acid bacteria of the genus Streptococcus, such as *Streptococcus faecalis* and *Streptococcus pyogenes*; lactic acid bacteria of the genus Enterococcus, such as *Enterococcus caseliflavus* and *Enterococcus sulfreus*; lactic acid bacteria of the genus Lactococcus, such as *Lactococcus plantarum* and *Lactococcus rafinolactis*; lactic acid bacteria of the genus Weissella, such as *Weissella confusa* and *Weissella kandleri*; Atopovium Lactobacillus species of the genus Atopobium, such as Atopobium minutum and Atopobium parvulus;Examples include lactic acid bacteria of the genus Vagococcus, such as Vagococcus fluvialis and Vagococcus salmoninarum; and lactic acid bacteria of the genus Pediococcus, such as Pediococcus damnosus and Pediococcus pentosaceus.

[0037] Examples of yeasts include Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, Saccharomyces bayonus, and other yeasts of the Saccharomyces genus; Galactomyces yeasts; Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei, and other yeasts of the Torulaspora genus; Zygosaccharomyces rouxii, Zygosaccharomyces Yeasts of the genus Zygosaccharomyces, such as Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, and Zygosaccharomyces lactis; yeasts of the genus Candida, such as Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scottii; Aureobasidium pullulans, Aureobasidium mansonii, and Aureobasidium Examples include yeasts of the genus Aureobasideium, such as Aureobasideium microstictum. Furthermore, the yeast according to the present invention may be any of the following: sake yeast, wine yeast, beer yeast, yeast derived from plant flowers (roses, lilies, cherry blossoms, etc.), or yeast derived from the sea.

[0038] Examples of koji mold include yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, and Aspergillus sojae; black koji molds such as Aspergillus awamori, Aspergillus kawauchii, Aspergillus usami, and Aspergillus niger; and red koji molds such as Monascus anka and Monascus pilosus.

[0039] When fermenting the above-mentioned rice or black soybeans with microorganisms, it is necessary to sterilize the material before the fermentation process to remove any unwanted bacteria that could hinder fermentation. As a method for sterilizing and removing these unwanted bacteria, the fermentation material may be washed beforehand with sterilizing ethanol or the like and then suspended in a sterile solvent such as sterile water. Alternatively, the fermentation material may be suspended in a solvent and then sterilized by heat sterilization or the like. Commonly used heat sterilization methods include autoclave sterilization, in which the suspension is heated at 120-130°C for 10-20 minutes, and intermittent sterilization, in which the suspension is kept at 80-90°C for 60-120 minutes, once a day for 2-3 days.

[0040] Next, the sterilized suspension is placed in a fermentation tank, and microorganisms are inoculated into it to induce fermentation. The amount of microorganisms inoculated is 10 7 ~10 8 The appropriate amount is cells / mL. If the inoculation amount exceeds the above range, the fermentation time remains largely unchanged. Conversely, if it falls below the above range, it takes a long time for fermentation to complete, which is undesirable.

[0041] The fermentation temperature is generally in the range of 5 to 50°C, preferably in the range of 20 to 40°C, which is the optimal growth temperature for each microorganism (for example, 30 to 40°C for lactic acid bacteria and 25 to 30°C for yeast). The fermentation period is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimal temperature. If the fermentation period is shorter than the above general range, fermentation will not occur sufficiently and the effectiveness of the fermented product tends to decrease. On the other hand, if it is extended beyond 10 days, not only will no further increase in effectiveness not be observed, but discoloration and an increase in fermentation odor will occur, both of which are undesirable.

[0042] Next, yeast extract can be prepared as follows. For example, yeast extract can be obtained by culturing yeast in a culture medium, by hydrolysis in which the cellular components of cultured yeast are solubilized with acid or alkali, by autolysis using proteolytic enzymes contained in the yeast cells, by enzymatic methods using enzyme preparations such as proteolytic enzymes, or by methods combining these. Yeast extract may also be concentrated or dried by conventional methods.

[0043] There are no particular limitations on the carbon source used when culturing yeast; examples of carbon sources include glucose, fructose, lactose, and raffinose. In addition to the carbon source, a nitrogen source may also be added; examples of nitrogen sources include amino acids and peptones.

[0044] The culture temperature for yeast is in the range of 25°C to 40°C, preferably 28°C to 35°C. The pH is in the range of 3.0 to 8.5, preferably 3.5 to 7.5.

[0045] Yeast extract concentrates can be obtained by concentrating a yeast culture medium, or a liquid obtained by hydrolysis with an acidic or alkaline solution, autolysis of yeast, or an enzymatic method, using a vacuum concentrator or the like.

[0046] Furthermore, dried yeast extracts can be obtained by drying the yeast culture solution or its concentrate using methods such as vacuum drying or spray drying.

[0047] Furthermore, the dried yeast extract may be prepared as a dried product to which excipients have been added for the purpose of improving chemical stability and low hygroscopicity. Excipients can include sugars such as starch, glucose, crystalline cellulose, lactose, and dextrin, as well as sugar alcohols such as sorbitol, xylitol, erythritol, mannitol, maltitol, and lactitol. Any substance that can be mixed with the yeast extract and dried into a powder is acceptable.

[0048] When incorporating the extract, hydrolysate, or fermented product of the present invention into a topical skin preparation, in addition to the essential active ingredient, other ingredients commonly used in topical skin preparations (cosmetics, quasi-drugs, or topical pharmaceuticals), such as oily components, surfactants (synthetic or natural), humectants, thickeners, emulsifiers or emulsifying aids, preservatives / bactericides, powder components, UV absorbers, antioxidants, metal ion chelating agents, pigments, fragrances, anti-wrinkle agents, pigmentation inhibitors, and other physiologically active ingredients, may be appropriately incorporated as needed.

[0049] Oily components include, for example, plant-derived oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, lemon peel oil, camphor bark oil, mandarin orange oil, cardamom oil, and orange oil; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; and liquid paraffin. Examples include hydrocarbons such as petrolatum, paraffin wax, and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, and higher fatty acid octyldodecyl (octyldodecyl stearate, etc.).

[0050] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, and polyoxyethylene Anionic surfactants such as ethylene alkylphenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammonium betaine, N,N,N-trialkyl-N-alkyleneammonium carboxybetaine, and N-acylamidopropyl-N′,N′-dimethyl-N′-β-hydroxypropylammonium sulfobetaine can be used.

[0051] As emulsifiers or emulsifying aids, the following may also be included: stevia derivatives such as enzyme-treated stevia, saponins or their derivatives, casein or its salts (sodium, etc.), sugar-protein complexes, sucrose or its esters, lactose, water-soluble polysaccharides derived from soybeans, complexes of soybean-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (such as enzyme-treated stevia), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their derivatives, lecithin and its derivatives (such as hydrogenated lecithin), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, grains, etc.), etc.

[0052] Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid ferment filtrate, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extracts, estradiol, various amino acids and their derivatives.

[0053] Examples of thickening agents include components derived from brown algae, green algae, or red algae such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin, pullulan, and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, and hydroxypropylcellulose; synthetic polymers such as carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, polyacrylic acid, etc.

[0054] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or its derivatives.

[0055] Examples of preservatives and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and aluminum bromide. These include chloroisoquinolinium, resorcinol, jamal (imidazodinylurea), isopropylmethylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, tree bark distillates, radish ferment filtrate, plant-derived ethanol such as sugarcane and corn, or 1,3-butylene glycol.

[0056] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0057] Examples of anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer 201, pyridoxine dicaprylate, and isopropylmethylphenol.

[0058] Examples of powder components include sericite, titanium dioxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powders, grain powders (rice, wheat, corn, millet, etc.), and legume powders (soybeans, adzuki beans, etc.).

[0059] Examples of UV absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0060] Examples of antioxidants include carotenoids such as butylhydroxyanisole, butylhydroxytoluene, propyl gallate, and astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), and vitamin A or its derivatives (e.g., retinol palmitate).

[0061] Examples of metal ion chelating agents include disodium edetate, trisodium edetate, tetrasodium edetate, etidronic acid or its salts (e.g., tetrasodium etidronate), gluconic acid or its salts (e.g., sodium gluconate), diethylenetriaminepentaacetic acid or its salts (e.g., pentasodium diethylenetriaminepentaacetic acid), hydroxyethanediphosphonic acid or its salts (e.g., tetrasodium hydroxyethanediphosphonic acid), and phytic acid or its salts (e.g., sodium phytate).

[0062] Examples of pigmentation preventatives include one or more selected from kojic acid or its derivatives, ascorbic acid or its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, resorcinol derivatives, potassium 4-methoxysalicylate, vitamin E or its derivatives, nicotinic acid or its derivatives, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), placental extract (placenta extract), and linoleic acid.

[0063] Examples of kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, as well as kojic acid ethers and kojic acid sugar derivatives such as kojic acid glucoside. Examples of ascorbic acid derivatives include L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, and L-ascorbic acid-2-sulfate. Ascorbic acid ester salts such as magnesium, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid), 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups include hexanoyl, octanoyl, and decanoyl groups), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralau Examples of hydroquinone derivatives include L-ascorbic acid tetra fatty acid esters such as phosphate esters, ascorbyl tetrahexyldecanoate, 3-O-ethyl ascorbic acid, and sodium L-ascorbic acid-2-phosphate-6-O-palmitate. Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside). Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate; examples of vitamin E derivatives include vitamin E nicotinate and vitamin E linoleate; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0064] Examples of anti-wrinkle agents include vitamin A or its derivatives, vitamin E or its derivatives (such as tocopherol acetate), and niacinamide.

[0065] The application sites for topical skin preparations (including cosmetics, quasi-drugs, and topical pharmaceuticals) containing extracts, hydrolysates, or fermented products according to the present invention include the entire skin, including the scalp, and are not particularly limited. Accordingly, examples of dosage forms include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, blushes, face powders, facial cleansers, body shampoos, soaps, mask preparations, and bath additives, but the present invention is not limited to these.

[0066] Next, the present invention will be described in more detail by examples of manufacturing, formulations, examples of implementation, and test examples, but the present invention is not limited thereto. In the following, all parts refer to parts by weight, and all percentages refer to weight percent.

[0067] Manufacturing Example 1. Preparation of Perilla Extract (1) 40g of dried perilla leaves were added to 400g of purified water and extracted at 10°C for 18 hours. This was filtered to obtain 336g of a brown extract (solids content 2.35%).

[0068] Manufacturing Example 2. Preparation of Perilla Extract (2) 40 g of dried perilla leaves were mixed with 400 g of a 30% 1,3-butylene glycol aqueous solution and extracted at 10°C for 18 hours. This was filtered to obtain 342 g of a brown extract (solids content 2.40%).

[0069] Manufacturing Example 3. Preparation of Perilla Extract (3) 40g of dried perilla leaves were added to 400g of a 20% ethanol aqueous solution and extracted at 10°C for 18 hours. This was filtered to obtain 348g of a brown extract (solids content 2.45%).

[0070] Production Example 4. Preparation of Rice Leaf Hydrolysate (1) 200 g of dried and pulverized rice leaves just before heading (panicle emergence stage) were added to 1000 g of purified water, and the mixture was extracted at 80°C for 1 hour. After filtration, 550 g of a pale yellow, transparent rice leaf extract solution (solid content concentration 2.5%) was obtained. 0.025 g of pectinase was added to 500 g of the obtained extract solution, and hydrolysis was carried out at 40°C for 4 hours. Subsequently, the enzyme was inactivated by heating at 90°C for 1 hour, and the mixture was filtered to obtain 460 g of a pale yellow, transparent enzyme hydrolysate solution of the rice leaf extract (solid content concentration 2.8%).

[0071] Manufacturing Example 5. Preparation of Rice Leaf Hydrolysate (2) Except for using cellulase instead of pectinase, the same procedure as in Production Example 5 was used to obtain 450 g of an enzymatic hydrolysate solution of rice leaf extract (solid content concentration 2.3%).

[0072] Production Example 6. Preparation of Rice Leaf Hydrolysate (3) Except for using xylanase instead of pectinase, the same procedure as in Production Example 5 was used to obtain 455 g of enzymatic hydrolysate of rice leaf extract (solid content concentration 2.5%).

[0073] Manufacturing Example 7. Preparation of Lotus Flower Extract 25 g of dried lotus flower parts (including petals, stamens, etc.) were powdered, and 5 g of the dried product was mixed with 500 g of purified water and 1,3-butylene glycol (1:1 ratio of purified water to 1,3-butylene glycol). Extraction was carried out at 40°C for 2 hours. After extraction, the solution was filtered to obtain 399 g of a dark brown, transparent lotus flower extract solution (solid content concentration 1.47%). Next, an aqueous potassium hydroxide solution was added to this extract solution to adjust the pH to 8.0. Then, a cation exchange resin (approximately 10 times the mass of solids in the extract solution) was added, and the mixture was stirred at room temperature for 18 hours and filtered to obtain a dark brown, transparent lotus flower extract solution (solid content concentration 1.34%).

[0074] Production Example 8. Preparation of Peony Extract Peony roots were dried, and 500g of purified water was added to 100g of the dried roots. The mixture was extracted at 4°C for 1 day. Furthermore, 500g of 1,3-butylene glycol was added, and the mixture was extracted at 4°C for 3 days. This was filtered to obtain 890g of yellowish-brown peony extract (solid content concentration 2.96%).

[0075] Manufacturing Example 9. Preparation of Angelica Extract The roots of Angelica acutiloba were dried, and 100g of the dried material was mixed with 500g of purified water and extracted at 40°C for 2 hours. Furthermore, 500g of 1,3-butylene glycol was added, and the mixture was extracted at 4°C for 3 days. This was filtered to obtain 960g of pale yellowish-brown Angelica acutiloba extract (solid content concentration 2.83%).

[0076] Preparation Example 10. Preparation of Tachibana Extract The pericarp of the tachibana orange was dried, and 500g of 1,3-butylene glycol was added to 100g of the dried material. The mixture was stirred and extracted at 60°C for 2 hours and then at 80°C for 1 hour. This was filtered, and 440g of purified water was added. This was filtered again, and 625g of an aqueous solution of 1,3-butylene glycol was added to obtain 1350g of pale yellowish-brown tachibana extract. (Solid content concentration 1.87%)

[0077] Preparation Example 11. Preparation of a mixture of peony extract, angelica extract, and mandarin orange extract. 800g of peony extract, 800g of angelica extract, and 400g of mandarin orange extract were mixed. This was filtered to obtain 1800g of a yellowish-brown mixed solution (solid content concentration 2.96%). Note that the mixing ratio of the extracts in production examples 8 to 10 is not limited to the mixing ratio in production example 12, and the solid content ratio can be adjusted to mandarin orange extract:peony extract = 1:1 to 5, or mandarin orange extract:angelica extract = 1:1 to 5.

[0078] Manufacturing Example 12. Preparation of Rice Hydrolyzate Solution (1) 250 g of polished rice was mixed with 1000 g of 0.1% sodium hydroxide aqueous solution, stirred and extracted for 1 day, and then roughly filtered through a filter cloth to remove the remaining rice residue. The extract was neutralized with dilute hydrochloric acid, and proteolytic enzymes (actinase AS 0.02%, papain 0.02%) were added relative to the volume of the extract. Enzymatic hydrolysis was carried out at 40°C for 2 hours, then heated at 80°C for 1 hour to inactivate the enzymes, and cooled to room temperature. The resulting enzyme-treated solution was purified and filtered to obtain 810 g of a light brown, transparent rice hydrolysate solution (solid content concentration: 1.40%).

[0079] Manufacturing Example 13. Preparation of Rice Hydrolyzate Solution (2) Except for using bromelain instead of papain as used in Production Example 8, 797 g of a light brown, transparent rice hydrolysate solution was obtained in the same manner as in Production Example 8 (solid content concentration: 1.41%).

[0080] Manufacturing Example 14. Preparation of Rice Fermented Products (1) 100g of polished rice was washed with water, 900g of purified water was added to prepare a suspension, and it was heat-sterilized. Yeast (Saccharomyces cerevisiae) was added to this liquid for 10 minutes. 8 The cells / mL were inoculated and incubated statically at 30°C for 3 days. After incubation, the culture medium was heated and sterilized, then cooled to room temperature to obtain a rice ferment (solid content concentration 2.5% by weight).

[0081] Manufacturing Example 15. Preparation of Soy Hydrolyzed Product (1) 10 g of dried and ground black soybean seeds were added to 200 g of purified water and extracted at 80°C for 1 hour. The resulting extract was coarsely filtered and adjusted to pH 5 with dilute hydrochloric acid. Then, lipase and protease (papain) were added to a concentration of 0.01%, and the mixture was allowed to react at 40°C for 3 hours. Next, the mixture was treated at 80°C for 1 hour to inactivate the enzymes, and then filtered to obtain 164 g of a light brown, transparent hydrolyzed black soybean extract solution (solid content concentration 1.00%).

[0082] Manufacturing Example 16. Preparation of Fermented Soybean Product 3 g of crushed soybean seeds were added to 297 g of purified water and heat-sterilized. 108 Lactobacillus delbrueckii cells / mL were inoculated into this suspension, and the mixture was incubated under a nitrogen stream at 37°C for 3 days. After incubation, the mixture was heat-sterilized, and the culture solution was filtered to obtain 203 g of fermented soybean seed solution (solid content 5.17%).

[0083] Manufacturing Example 17. Preparation of Eggplant Extract (1) 2800g of eggplant (water eggplant) fruit was processed into a paste using a food processor, and the resulting liquid was filtered to obtain 2010g of a clear, yellowish-brown eggplant extract (solid content concentration 4.10%).

[0084] Manufacturing Example 18. Preparation of Eggplant Extract (2) 100g of dried eggplant (Kamo eggplant) fruit was mixed with 1000g of purified water, extracted at 40°C for 4 hours, and then filtered to obtain 800g of a clear yellow watery eggplant fruit extract (solid content concentration 1.50%).

[0085] Manufacturing Example 19. Preparation of Yeast Extract 2700g of sterilized GP liquid medium was mixed with 300g of pre-cultured yeast (Saccharomyces cerevisiae) that had been cultured in the same medium beforehand, and incubated at 30°C for 20 hours with aeration and stirring. After heat sterilization, the pH was adjusted to 8.5 with sodium hydroxide solution, and the mixture was heat-treated at 90°C for 3 hours with stirring. After pH adjustment of this solution, it was filtered to obtain a yeast culture extract (1604g) (solid content concentration 1.12%).

[0086] The present invention aims to find an active ingredient that is applicable to the skin and suppresses or restores the decline in lymphatic vessel function. Therefore, focusing on "integrin α9" and "chemokine CCL21" related to lymphatic vessel function, the effect of suppressing or restoring the decline in lymphatic vessel function was evaluated by the following method.

[0087] Test Example 1: Effect of promoting integrin α9 synthesis in lymphoid endothelial cells Human cutaneous lymphoid endothelial cells (HDLEC Adult) were placed in a 96-well microplate containing endothelial cell proliferation medium MV2 kit (Takara Bio) in 1 × 10⁶ units. 4 Cells were seeded per hole and pre-cultured for 1 day at 37°C and 5.0% CO2. Then, the same medium containing the prepared sample solution was added, and the cells were cultured for a further 3 days under the same conditions. Here, the sample solutions used were extracts from Production Example 1, hydrolysate from Production Example 4, extracts from Production Examples 7-10, and a mixture from Production Example 11, respectively, with their concentrations adjusted so that the final concentration as a solution in the medium was 1.0%. After culturing for 3 days with the sample solution added, the cultured cells were fixed by treating them with 15% neutral formalin solution for 30 minutes, and then blocked by 5-fold dilution of Blocking One P solution at 4°C overnight. After blocking, anti-integrin α9 antibody was added and gently mixed at room temperature for 2 hours. Subsequently, the cells were washed with 0.2% Triton / PBS(-), fluorescently labeled secondary antibody (Alexa Fluor546) was added, and the cells were gently mixed in the dark for a certain period of time. Afterward, the samples were washed, and the fluorescence intensity was measured at Ex=544nm and Em=590nm (using a fluorescence microplate reader (Fluoroscan Ascent, Thermo Fisher Scientific)). DNA staining with Hoechst33342 was also performed, and measurements were taken at Ex=355nm and Em=460nm. The degree of integrin α9 synthesis was determined by dividing the fluorescence intensity of Alexa Fluor546 in each test group by the fluorescence intensity of Hoechst33342. The same procedure was also performed for samples without the addition of the above-mentioned solvent (control), and the relative value of the degree of integrin α9 synthesis with each sample solution added to the degree of integrin α9 synthesis obtained here was calculated and expressed as the integrin α9 synthesis amount (%).

[0088] The results of Test Example 1 are shown in Figure 1. As shown in Figure 1, it was confirmed that the extract of Production Example 1, the hydrolysate of Production Example 4, the extracts of Production Examples 7-10, and the mixture of Production Example 11 all have the effect of promoting the synthesis of integrin α9 in lymphoid endothelial cells. Here, "integrin α9," as shown in Non-Patent Literature 1 above, for example, is present in lymphatic valve cells and plays the role of a ligand that adheres the extracellular matrix to lymphoid endothelial cells. From this, it is thought that when "integrin α9" decreases in lymphoid endothelial cells, the structure of lymphatic vessels in the dermis becomes unstable, and as a result, the important function of lymphatic vessels, which is to drain tissue fluid and excess water from the dermis, decreases, leading to dermal deterioration and edema. Furthermore, it was confirmed that the extract from Production Example 1, the hydrolysate from Production Example 4, the extracts from Production Examples 7-10, and the mixture from Production Example 11 according to the present invention have the effect of promoting integrin α9 synthesis. Therefore, it is suggested that the present invention, which uses these as active ingredients, can be applied to the skin to promote integrin α9 synthesis, thereby suppressing or restoring lymphatic vessel dysfunction and improving dermal deterioration, edema, etc.

[0089] Test Example 2. Evaluation test of the effect of promoting the synthesis of the chemokine CCL21 in lymphoid endothelial cells. Human cutaneous lymphoid endothelial cells (HDLEC Adult) were placed in a 96-well microplate containing endothelial cell proliferation medium MV2 kit (Takara Bio) in 1 × 10⁶ units. 4Cells were seeded per well and pre-cultured for 1 day at 37°C and 5.0% CO2. Then, the same medium containing the prepared sample solution was added, and the cells were cultured for a further 3 days under the same conditions. For the sample solution, the hydrolysate from Production Example 12, the hydrolysate from Production Example 15, the extract from Production Example 17, and the extract from Production Example 19 were used, and their concentrations were adjusted so that the final concentration as a solution in the medium was 1.0%. Subsequently, the amount of chemokine CCL21 in the culture supernatant was measured using the 6Ckine / CCL21 Human ELISA Kit (Invitrogen). In addition, the same procedure was performed using human cutaneous lymphoid endothelial cells (HDLEC Adult) and human cutaneous lymphoid endothelial cells (HDLEC Juvenile) in the case of no sample added (control) by adding the same concentration of solvent instead of the above sample solution. The test results were calculated by determining the relative degree of chemokine CCL21 synthesis with each sample added to human cutaneous lymphoid endothelial cells (HDLEC Adult) without sample addition, or the relative degree of chemokine CCL21 synthesis in human cutaneous lymphoid endothelial cells (HDLEC Juvenile), and expressing it as the amount of chemokine CCL21 synthesized (%).

[0090] The results of Test Example 2 are shown in Figure 2. As shown in Figure 2, it was confirmed that the hydrolysates of Production Example 12, Production Example 15, Production Example 17, and Production Example 19 according to the present invention all have the effect of promoting the secretion of the chemokine CCL21 from lymphoid endothelial cells. Here, "chemokine CCL21" is a chemokine (a substance that has the ability to migrate inflammatory cells, etc.) secreted from lymphatic vessels, and it is known that inflammatory cells, etc. are guided into the lymphatic vessels and discharged from the dermis by this chemokine CCL21. If inflammatory cells are not guided into the lymphatic vessels and remain in the dermis, phenomena such as the breakdown of the extracellular matrix occur, which is thought to not only impair the function of lymphatic vessels but also cause skin aging (wrinkles, sagging, etc.). Furthermore, as shown in Figure 2, it was confirmed that the secretion of chemokine CCL21 was reduced in adult-derived lymphatic cells (HDLEC Adult) compared with young-derived cells (HDLEC juvenile). In other words, it was suggested that the secretion of the chemokine CCL21 decreases with age. Furthermore, since the hydrolysates of Production Example 12, Production Example 15, Production Example 17, and Production Example 19 of the present invention have the effect of promoting the secretion of the chemokine CCL21 by lymphatic endothelial cells, it is suggested that the present invention, which uses these as active ingredients, can, when applied to the skin, induce inflammatory cells to the lymphatic vessels, prevent the accumulation of inflammatory cells in the dermis, and promote the suppression or recovery of lymphatic vessel function decline. It is also suggested that it can prevent skin aging associated with lymphatic vessel function decline (e.g., age spots, wrinkles, sagging, etc.).

[0091] Prescription example 1. Lotion [Ingredients] Part Squalane 0.1 Extract from Production Example 1: 1.0 Fermented product from manufacturing example 14: 10.0 Dipotassium glycyrrhizinate 0.5 Sodium citrate 0.1 Citric acid 0.1 Phenoxyethanol 0.02 Pentanediol 0.02 1,3-Butylene glycol 0.1 Purified water, in an amount that makes the total volume 100 parts

[0092] Prescription example 2. Lotion In the lotion of Formulation Example 1, a lotion was obtained with the same composition as Formulation Example 1, except that hydrolyzed product 1.0 of Production Example 4 was used instead of the extract of Production Example 1.

[0093] Prescription example 3. Lotion In the lotion of Formulation Example 1, a lotion was obtained with the same composition as Formulation Example 1, except that the extract 1.0 of Manufacturing Example 7 was used instead of the extract of Manufacturing Example 1.

[0094] Prescription example 4. Lotion In the lotion of Formulation Example 1, a lotion was obtained with the same composition as Formulation Example 1, except that mixture 1.0 of Production Example 11 was used instead of the extract of Production Example 1.

[0095] Prescription example 5. Lotion In the lotion of Formulation Example 1, a lotion was obtained with the same composition as Formulation Example 1, except that hydrolyzed product 1.0 of Production Example 12 was used instead of the extract of Production Example 1.

[0096] Prescription example 6. Lotion In the lotion of Formulation Example 1, a lotion was obtained with the same composition as Formulation Example 1, except that hydrolyzed product 1.0 of Production Example 15 was used instead of the extract of Production Example 1.

[0097] Prescription example 7. Lotion In the lotion of Formulation Example 1, a lotion was obtained with the same composition as Formulation Example 1, except that the extract 1.0 of Production Example 17 was used instead of the extract of Production Example 1.

[0098] Prescription example 8. Lotion In the lotion of Formulation Example 1, a lotion was obtained with the same composition as Formulation Example 1, except that the extract 1.0 from Production Example 19 was used instead of the extract from Production Example 1.

[0099] Prescription example 9. Cream [Ingredients] Part Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl isostearate 5.0 Tri(caprylic / caprin / myristic / stearic acid) glyceryl 1.0 Methylphenylpolysiloxane 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 1.0 Niacinamide 5.0 Extract from Production Example 1: 1.0 Hydrolyzed product of manufacturing example 15: 1.0 Behenyl alcohol 2.0 Lactic acid fermented rice 2.0 Hydrogenated lecithin 0.5 Xanthan gum 1.0 Carboxyvinyl polymer 0.01 Titanium dioxide 0.01 1,3-Butylene glycol 3.0 Glycerin 0.05 Phenoxyethanol 0.1 Purified water, in an amount that makes the total volume 100 parts

[0100] Prescription example 10. Cream In the cream of Formulation Example 9, a cream was obtained with the same composition as Formulation Example 9, except that hydrolyzed product 1.0 of Production Example 4 was used instead of the extract of Production Example 1.

[0101] Prescription example 11. Cream In the cream of Formulation Example 9, a cream was obtained with the same composition as Formulation Example 8, except that the extract 1.0 from Production Example 7 was used instead of the extract from Production Example 1.

[0102] Prescription example 12. Cream In the cream of Formulation Example 9, a cream was obtained with the same composition as Formulation Example 9, except that the mixture 1.0 of Production Example 11 was used instead of the extract of Production Example 1.

[0103] Prescription example 12. Cream In the cream of Formulation Example 9, a cream was obtained with the same composition as Formulation Example 9, except that hydrolyzed product 1.0 of Production Example 12 was used instead of the extract of Production Example 1.

[0104] Prescription example 13. Cream In the cream of Formulation Example 9, a cream was obtained with the same composition as Formulation Example 9, except that the fermented product 1.0 of Production Example 16 was used instead of the extract of Production Example 1.

[0105] Prescription example 14. Cream In the cream of Formulation Example 9, a cream was obtained with the same composition as Formulation Example 9, except that the extract 1.0 from Manufacturing Example 17 was used instead of the extract from Manufacturing Example 1.

[0106] Prescription example 15. Cream In the cream of Formulation Example 9, the extract 1.0 from Production Example 18 was used instead of the extract from Production Example 1, except that the cream was obtained with the same composition as Formulation Example 9.

[0107] Prescription example 16. Cream In the cream of Formulation Example 9, a cream was obtained with the same composition as Formulation Example 8, except that the extract 1.0 from Production Example 19 was used instead of the extract from Production Example 1.

[0108] Prescription example 17. Cream In the cream of Formulation Example 9, 1.0 part of tranexamic acid and 0.1 part of sodium pyrosulfite were used instead of 5.0 parts of niacinamide, except that a cream with the same composition as Formulation Example 8 was obtained.

[0109] Prescription example 18. Emulsion [Ingredients] Part Squalane 5.0 Hexaran 3.0 Hexyldecyl isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 laurate 5.0 Polyglyceryl-10 isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Niacinamide 5.0 Ascorbic acid glucoside 2.0 Tranexamic acid 2.0 Dipotassium glycyrrhizinate 0.1 Arbutin 3.0 Extract from Production Example 1: 1.0 Extract from manufacturing example 19: 1.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Isopropylmethylphenol 0.1 Water-soluble collagen 0.1 Hydrolyzed collagen 0.1 Sodium hyaluronate 0.01 Glycerin 3.0 1,3-Butylene glycol 2.0 1,2-Hexanediol 0.1 1,3-Propanediol 0.1 Potassium hydroxide (appropriate amount) Purified water, in an amount that makes the total volume 100 parts

[0110] Prescription example 19. Emulsion An emulsion was obtained with the same composition as in Formula Example 18, except that 2.0 parts of magnesium L-ascorbyl phosphate were used instead of 2.0 parts of ascorbic acid glucoside in the emulsion of Formula Example 18.

[0111] Prescription example 20. Emulsion An emulsion was prepared in the same manner as in Formula Example 18, except that 2.0 parts of 3-O-ethyl ascorbic acid were used instead of 2.0 parts of ascorbic acid glucoside in the emulsion of Formula Example 18.

[0112] Prescription example 21. Emulsion An emulsion was prepared in the same manner as in Formula Example 18, except that 3.0 parts of glyceryl ascorbate were used instead of 2.0 parts of glucoside ascorbate.

[0113] Prescription example 22. Emulsion An emulsion was prepared in the same manner as in Prescription Example 18, except that 3.0 parts of ascorbyl tetrahexyldecanoate were used instead of 2.0 parts of ascorbic acid glucoside in the emulsion of Prescription Example 18.

[0114] Prescription example 23. Pack [Ingredients] Part Dipropylene glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetanol 3.0 Behenyl alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.1 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Niacinamide 5.0 Hydrolyzed product of manufacturing example 4: 1.0 Hydrolyzed product of manufacturing example 12: 1.0 Xanthan gum 2.0 Polyglyceryl-6 myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated lecithin 3.0 Hydroxylated lecithin 3.0 Purified water, in an amount that makes the total volume 100 parts

[0115] Prescription Example 24: Hair Shampoo [Ingredients] Part Sodium laureth sulfate 10.0 Glyceryl monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl alcohol 2.0 Behenyl alcohol 2.0 Dimethicone 3.0 Niacinamide 1.0 Extract from manufacturing example 7: 1.0 Extract from manufacturing example 17: 1.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene glycol 2.0 Purified water, in an amount that makes the total volume 100 parts

[0116] Prescription Example 25: Hair Conditioner [Ingredients] Part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetanol 3.0 Stearyl alcohol 1.0 Niacinamide 1.0 Mixture of Production Example 11 1.0 Extract from manufacturing example 19: 1.0 Allantoin 0.1 Isopropylmethylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water, in an amount that makes the total volume 100 parts

[0117] Prescription Example 26. Cleansing Cosmetics [Ingredients] Part Potassium cocoyl glycine 5.0 Glycerin 10.0 Glyceryl caprylate 1.0 Sodium lauroyl aspartate 10.0 Niacinamide 5.0 Extract from Production Example 1: 1.0 Hydrolyzed product of manufacturing example 12: 1.0 Cetanol 3.0 Myristyl alcohol 3.0 Isopropylmethyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.05 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.1 Triclosan 0.1 Trichlorocarbanide 0.5 Trichlorohydroxydiphenyl ether 0.2 Concentrated benzalkonium chloride solution 50 0.2 Benzalkonium chloride 0.1 Purified water, in an amount that makes the total volume 100 parts

[0118] Prescription Example 27: Sheet Mask A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients. [Ingredients] Part Glycerin 3.0 L-ascorbic acid 2-glucoside 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Niacinamide 5.0 Extract from Production Example 1: 0.2 Hydrolyzed product of manufacturing example 4: 0.2 Extract from manufacturing example 7: 0.2 Mixture of Production Example 11 0.2 Hydrolyzed product of manufacturing example 12: 0.2 Hydrolyzed product of manufacturing example 16: 0.2 Extract from manufacturing example 17: 0.2 Extract from manufacturing example 19: 0.2 1,3-Butylene glycol 2.0 Hexanediol 0.1 Potassium hydroxide (appropriate amount) Purified water, in an amount that makes the total volume 100 parts

[0119] Prescription example 26. Serum [Ingredients] Part Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Hyaluronic acid hydrolysate 0.1 Water-soluble collagen 0.1 Ceramide 0.1 Niacinamide 3.0 Extract from Production Example 1: 0.2 Hydrolyzed product of manufacturing example 4: 0.2 Extract from manufacturing example 7: 0.2 Mixture of Production Example 11 0.2 Hydrolyzed product of manufacturing example 12: 0.2 Hydrolyzed product of manufacturing example 16: 0.2 Extract from manufacturing example 17: 0.2 Extract from manufacturing example 19: 0.2 Citric acid 0.3 Sodium citrate 0.3 Purified water, in an amount that makes the total volume 100 parts

Claims

[Claim 1] A topical skin composition for suppressing or restoring lymphatic vessel dysfunction, comprising one or more of the following as active ingredients: perilla extract, rice leaf hydrolysate, lotus extract, peony extract, angelica extract, mandarin orange extract, rice extract or its hydrolysate or fermented rice product, yeast extract, black soybean hydrolysate, fermented soybean product, and eggplant extract.

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