Bamboo sap particle composite, method for producing the same, and use

Abstract

This invention relates to a bamboo sap particle complex, a method for producing the same, and its use. The bamboo sap particle complex comprises bamboo sap and an inorganic ion mixture. The method for producing the bamboo sap particle complex involves mixing the bamboo sap and the inorganic ion-containing mixture in an aqueous solution, stirring and centrifuging to remove the precipitate, and using the bamboo sap particle complex in a hair growth product. The advantage is that the bamboo sap particle complex has a better hair growth effect compared to minoxidil. The hair growth effect of the bamboo sap particle complex of this invention is close to that of bamboo sap with a relatively high mass concentration. This indicates that the bamboo sap particle complex can improve the skin penetration of trace amounts of bamboo sap, effectively promote the secretion of IGF-1, VEGF, and FGF-2, and enhance the hair growth effect of trace amounts of bamboo sap.

Description

[Technical Field]

[0001] This invention relates to the field of biotechnology, and more particularly to bamboo sap particle composites, methods for producing the same, and their use. [Background technology]

[0002] Hair is an appendage of the skin, and influenced by factors such as genetics, environment, and workplace stress, modern people face the risk of early and rapid hair loss, which has a serious impact on their work and studies.

[0003] Minoxidil (topical solution) is currently the only medication available worldwide for treating hair loss. It has 35 years of clinical experience since its approval by the U.S. Food and Drug Administration in August 1988. However, it does not work for everyone; according to FDA data, the effectiveness of minoxidil is 39%, and it is limited to cases of alopecia on the crown of the head. At the same time, the following side effects may occur during the use of minoxidil solution to treat hair loss: 1. Hirsutism, particularly noticeable on the face, neck, upper and lower extremities; 2. Tachycardia; 3. Allergic reactions, including localized redness and itching of the skin.

[0004] Considering the side effects of minoxidil and the need to improve the effectiveness of hair loss treatment, many hospital dermatology departments directly inject insulin-like growth factor 1 (IGF-1), vascular endothelial growth factor (VEGF), or basic fibroblast growth factor (FGF-2) into the areas of hair loss. However, this treatment is not only expensive, but direct injection can cause wounds and scars on the scalp, and postoperative symptoms such as bruising, swelling, and pain may occur. Furthermore, such treatments require a relatively long treatment period of six months to a year. Currently, proteins produced using genetic engineering technology (IGF-1, VEGF, and FGF-2) have a relatively short period of clinical use, and their safety for human use needs to be further confirmed.

[0005] In China, bamboo is a very common plant and has long been considered to have important ecological functions. It reduces soil erosion and can store large amounts of carbon and carbon dioxide, meaning it has a strong carbon sequestration capacity. Furthermore, bamboo can absorb wastewater and reduce metal toxicity in the soil. Bamboo also has certain benefits for human health and can be used to control diseases such as diabetes, high cholesterol, and high blood pressure. Conventional techniques have shown that applying bamboo sap alone to areas of hair loss promotes hair growth compared to a blank control group with no treatment, but the hair growth effect of bamboo sap is not yet widely utilized. Possible reasons for this include: firstly, a lack of scientific experimental proof and insufficient verification of the mechanism; secondly, low skin penetration of the active ingredients; and thirdly, the fact that the unique odor of bamboo sap is unacceptable to users.

[0006] In existing hair loss treatments, minoxidil has a treatment efficiency of only 39% and is limited to cases of hair loss on the crown of the head, making it inefficient. Furthermore, treatments such as directly injecting the hair growth factor IGF-1 into the scalp are not only expensive but also lack established reliability, necessitating the use of new treatment methods to replace these existing methods. Bamboo sap has low skin penetration of its active ingredients, often requiring large quantities to be effective, and its distinctive odor is unacceptable to users. Moreover, the reliability of hair growth using bamboo sap has not been proven through scientific experiments, and therefore it is not widely used. For the reasons stated above, this application proposes a bamboo sap particle complex, a method for manufacturing the same, and its use in order to improve existing hair loss treatments. [Overview of the project]

[0007] In existing hair loss treatments, minoxidil has a treatment efficiency of only 39%, is limited to cases of hair loss on the crown of the head, and is not highly effective. Furthermore, treatments such as directly injecting the hair growth factor IGF-1 into the scalp are not only expensive but also lack established reliability. Bamboo sap has hair growth effects, but its active ingredients have low skin penetration, so it is often necessary to use large quantities of bamboo sap to see results. Its unique odor is also unacceptable to users, and the reliability of hair growth using bamboo sap has not been proven through scientific experiments, thus it is not widely used. This application provides a bamboo sap particle complex, a method for producing the same, and its use for treating hair loss.

[0008] The above-described object of the present invention is realized in the following forms.

[0009] In the first aspect, the present invention provides a bamboo sap particle composite comprising bamboo sap and an inorganic ion mixture, wherein the bamboo sap and the inorganic ion mixture form a particle composite.

[0010] Preferably, the inorganic ion mixture is Ca 2+ CO3 2- , PO4 3- Cl - kaNa + Includes.

[0011] More preferably, the inorganic ion mixture is carbonate apatite particles.

[0012] Preferably, the relative content of bamboo sap and inorganic ion mixture is 1-16% and 84-99% by mass.

[0013] More preferably, the relative content of the bamboo sap and inorganic ion mixture is 3-4% and 96-97% by mass.

[0014] More preferably, the relative content of the bamboo sap and inorganic ion mixture is 3.25% and 96.75% by mass.

[0015] The bamboo liquid particle complex is used for manufacturing a hair growth product.

[0016] The bamboo liquid particle complex is used for hair growth.

[0017] In a second aspect, the present invention provides a method for manufacturing the bamboo liquid particle complex according to the first aspect, comprising:

[0018] S1. A step of preparing a preliminary preparation liquid containing bamboo liquid and an inorganic ion-containing mixture with distilled water;

[0019] S2. A step of stirring the preliminary preparation liquid prepared in S1, centrifuging after uniformly stirring, and the obtained precipitate becomes the bamboo liquid particle complex. The present invention provides a method for manufacturing a bamboo liquid particle complex.

[0020] Preferably, in S1, by mass%, the bamboo liquid content is 1.5 - 25%, and the inorganic ion-containing mixture is 100 - 2500 mM;

[0021] [[ID=,24]]In S2, the stirring temperature is 4 - 37 °C, the stirring time is 1 - 30 min, the stirring time decreases as the stirring temperature rises, and centrifugation is carried out at 3000 - 5000 rpm for 2 - 5 min.

[0022] More preferably, in S1, by mass%, the bamboo liquid content is 5%, and the inorganic ion-containing mixture contains 440 mM of NaHCO3, 9 mM of NaH2PO4, and 58 mM of CaCl2;

[0023] In S2, the stirring temperature is 20 °C, the stirring time is 3 min, and centrifugation is carried out at 4000 rpm for 3 min.

[0024] In a third aspect, the present invention provides the use of the bamboo liquid particle complex according to the first aspect for manufacturing a hair growth product.

[0025] In the fourth aspect, the present invention provides a method for promoting hair growth, comprising administering an effective amount of the bamboo sap particle complex described in the first aspect to a subject requiring it. The advantageous technical effects of the present invention are as follows:

[0026] The bamboo sap particle complex of the present invention, its manufacturing method, and its use demonstrate that the bamboo sap particle complex exhibits a better hair growth effect compared to minoxidil. Furthermore, the hair growth effect of the bamboo sap particle complex is close to that of bamboo sap at a relatively high mass concentration. This indicates that the bamboo sap particle complex can improve the skin penetration of trace amounts of bamboo sap, effectively promote the secretion of IGF-1, VEGF, and FGF-2, and enhance the hair growth effect of trace amounts of bamboo sap. [Brief explanation of the drawing]

[0027] [Figure 1a] This is a TEM transmission electron microscope (TEM) observation of the bamboo sap particle composite in an embodiment of the present invention (1). [Figure 1b] This is a TEM transmission electron microscope (TEM) observation of the bamboo sap particle composite in an embodiment of the present invention (2). [Figure 2] This figure shows the measurement results of a bamboo sap particle composite in an embodiment of the present invention, measured using a Malvern particle size analyzer. [Figure 3] This is a diagram showing the hair growth status on the back of a mouse in Experimental Example 1 of the present invention. [Figure 4] This is a piecewise statistical graph showing the change in area of ​​mouse hair in Experimental Example 1 of the present invention. [Figure 5] This is a statistical diagram of hair growth for AUC-Day12 and AUC-Day16 in Experimental Example 1 of the present invention. [Figure 6] This is an immunohistochemical expression diagram of IGF-1 in Experimental Example 1 of the present invention. [Figure 7] This is an immunohistochemical expression diagram of VEGF in Experimental Example 1 of the present invention. [Figure 8] This is an immunohistochemical expression diagram of FGF-2 in Experimental Example 1 of the present invention. [Figure 9] This is a diagram illustrating the experimental process for Experimental Example 2 of the present invention. [Figure 10a]1H NMR spectrum of Solution A in Experimental Example 2 of the present invention. [Figure 10b] 1H NMR spectrum of Solution B in Experimental Example 2 of the present invention. [Figure 11] Diagram of the hair growth situation on the back of a mouse in Experimental Example 3 of the present invention. [Figure 12] Line statistical chart of the area change of mouse hair in Experimental Example 3 of the present invention. [Figure 13] Hair growth statistical chart of AUC-Day14 in Experimental Example 3 of the present invention.

Mode for Carrying Out the Invention

[0028] [[ID=1⑨]] Hereinafter, referring to the drawings of the embodiments of the present application, the configurations of the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, not all of the embodiments. Based on the embodiments of the present application, any other embodiments made by those skilled in the art without creative efforts are all included in the scope of rights of the present application.

Embodiment

[0029] In a first aspect, the present invention provides a bamboo liquid particle complex containing a bamboo liquid and an inorganic ion mixture, which forms a particle complex with the bamboo liquid and the inorganic ion mixture.

[0030] Among them, the inorganic ion mixture contains Ca 2+ , CO3 2- , PO4 3- , Cl - , Na + . [

[0031] Furthermore, the inorganic ion mixture is carbonated apatite particles.

[0032] Carbonated apatite has a structure obtained by substituting some hydroxyl groups of hydroxyapatite (Ca 10 (PO4)6(OH)2) with CO3 groups, and the general formula is Ca 10-m X m (PO4)6(CO3) 1-nY n The compound is represented by the formula. In the formula, X is an element that can partially substitute for Ca in carbonate apatite, preferably Na, Sr, Mn, or a rare earth element. m is usually a number between 0 and 1, preferably between 0 and 0.1, more preferably between 0 and 0.01, and even more preferably between 0 and 0.001. Y is a group or element that can partially substitute for CO3 in carbonate apatite, preferably OH, F, or Cl. n is usually a number between 0 and 0.1, preferably between 0 and 0.01, more preferably between 0 and 0.001, and even more preferably between 0 and 0.0001.

[0033] Of this, the relative content of bamboo sap and inorganic ion mixture is 1-16% and 84-99% by mass, respectively.

[0034] In some examples, the relative content of bamboo sap and carbonate apatite particles is 1% and 99% by mass, respectively.

[0035] In some examples, the relative content of bamboo sap and carbonate apatite particles is 16% and 84% by mass, respectively.

[0036] In some examples, the relative content of bamboo sap and carbonate apatite particles is 8% and 92% by mass, respectively.

[0037] Preferably, the relative content of bamboo sap and inorganic ion mixture is 3-4% and 96-97% by mass.

[0038] More preferably, the relative content of the bamboo sap and inorganic ion mixture is 3.25% and 96.75% by mass.

[0039] In the second aspect, the present invention relates to a method for producing the bamboo sap particle composite described in the first aspect,

[0040] S1, a step of preparing a preliminary solution containing bamboo sap and an inorganic ion-containing mixture with distilled water,

[0041] The present invention provides a method for producing a bamboo sap particle composite, comprising the steps of: stirring the pre-prepared solutions prepared in S2 and S1 to ensure uniform mixing, then centrifuging, and the resulting precipitate becoming the bamboo sap particle composite.

[0042] In S1, the bamboo sap content is 1.5-25% by mass, and the inorganic ion-containing mixture is 100-2500 mM. In S2, the stirring temperature is 4-37°C, the stirring time is 1-30 min, the stirring time decreases as the stirring temperature increases, and the mixture is centrifuged at 3000-5000 rpm for 2-5 min.

[0043] In some examples, in S1, the bamboo sap content is 1.5% by mass, and the inorganic ion-containing mixture is 100 mM. In S2, the stirring temperature is 4°C, the stirring time is 1 min, the stirring time decreases as the stirring temperature increases, and the mixture is centrifuged at 3000 rpm for 2 min.

[0044] In some examples, in S1, the bamboo sap content is 25% by mass, and the inorganic ion-containing mixture is 2500 mM. In S2, the stirring temperature is 37°C, the stirring time is 30 min, the stirring time decreases as the stirring temperature increases, and the mixture is centrifuged at 5000 rpm for 5 min.

[0045] Application Examples

[0046] The bamboo sap particle composite comprises bamboo sap and carbonate apatite particles, with the bamboo sap and carbonate apatite particles forming a particle composite, and the relative mass content of bamboo sap and carbonate apatite particles being 3.25% and 96.75%, respectively.

[0047] The method for producing the above-mentioned bamboo sap particle composite includes the following steps.

[0048] S1. Prepare 100 mL of an aqueous solution containing bamboo sap and an inorganic ion-containing mixture, of which the bamboo sap content is 5% by mass. Add 440 mM NaHCO3, 9 mM NaH2PO4, and 58 mM CaCl2.

[0049] After stirring at 20°C for 3 minutes, the mixture is centrifuged at 4000 rpm for 3 minutes, and the resulting precipitate is the bamboo sap particle composite.

[0050] In particular, the bamboo sap may be selected from one or a combination of the following: bamboo charcoal ash extract, bamboo ethanol infusion, bamboo vinegar, or boiled bamboo leaf extract.

[0051] As one example, the method for producing bamboo sap may be selected from the following options.

[0052] 1. The bamboo is crushed and dried as necessary, then dry-distilled under reduced pressure conditions, and the distillate is collected. The reduced pressure conditions are 100 mmHg or less, preferably 10 to 60 mmHg, and more preferably 15 to 45 mmHg. During reduced-pressure dry distillation, the distillation temperature of the dry distillation product depends on the degree of reduced pressure; it is lower when the degree of reduced pressure is high and higher when the degree of reduced pressure is low. For example, when dry distillation is performed under reduced pressure at 20 mmHg, the distillation temperature of the dry distillation product is 180 to 200°C.

[0053] 2. The crushed bamboo is subjected to vacuum carbonization in a vacuum distillation apparatus, and the distillate is carbonized under conditions of approximately 200°C and 20 mmHg, and the distillate is diluted with ethanol.

[0054] 3. A simple purification method for crude bamboo vinegar mainly uses the natural sedimentation method, where crude bamboo vinegar is placed in a container and left to stand for a certain period of time, allowing it to naturally separate into layers. The upper layer is a light-colored, clear bamboo vinegar, and the lower layer is a dark brown bamboo tar. Alternatively, benzene and vanadium chloride, which are organic solvents, can be mixed and stirred with the bamboo vinegar in different proportions. After standing, two layers will form. After separating the two layers, the solution can be taken out, and the solvent can be extracted and recovered by distillation. Currently, other simple purification methods are also used. For example, adsorption purification methods using charcoal, activated carbon, or other materials are used, where charcoal, bamboo charcoal, activated carbon, etc., are mixed and stirred with crude bamboo vinegar in a certain proportion, left to stand, and then filtered.

[0055] In one select embodiment of the present invention, the method for producing the bamboo sap includes crushing the bamboo, optionally drying the bamboo, dry distilling the bamboo under reduced pressure, and collecting the distillate.

[0056] Optionally, the reduced pressure conditions for the carbonization may be 100 mmHg or less, and may be 10-60 mmHg, 15-45 mmHg, or 20 mmHg.

[0057] The carbonization temperature is arbitrarily 180-200°C, and arbitrarily 200°C.

[0058] Optionally, the bamboo sap production method further includes a step of distilling the distillate and / or a step of vacuum filtration of the distillate.

[0059] Optionally, the bamboo sap production method further comprises diluting the distillate with ethanol.

[0060] The present invention provides a use for manufacturing a hair growth product using the manufactured bamboo sap particle complex.

[0061] The above-mentioned hair growth product includes a hair growth liquid, a hair growth ointment, and a hair growth spray. By adding the bamboo sap particle complex of this invention to the hair growth product, a remarkable hair growth effect can be achieved.

[0062] In one of these uses, the precipitate produced by the above method is dissolved in 50 mL of distilled water, placed in a spray bottle, and sprayed onto the area where hair growth is desired to promote hair growth.

[0063] Verification of the morphology and particle size of bamboo sap particle composites

[0064] The morphology of the bamboo sap particle composite was observed using a TEM transmission electron microscope. As shown in Figures 1a and 1b, Figure 1a shows spherical particles smaller than 100 nm, and Figure 1b shows micron aggregates composed of nanoparticles (400-800 nm). After measurement using a Malvern particle size analyzer, as shown in Figure 2, the particle size distribution of the bamboo sap particle composite was d(0.1): 4.347 μm, d(0.5): 29.247 μm, and d(0.9): 51.978 μm.

[0065] Experimental Example 1: Research experiment on the effect of bamboo sap particle complex on hair growth in mice

[0066] Experimental mouse: C57BL / 6 male, 7 weeks old

[0067] Experimental environment: Temperature 20-26°C, humidity 40-70%

[0068] Experimental grouping: The experiment was divided into four groups, with three animals per group.

[0069] 1) is the control group (no treatment was applied).

[0070] 2) is a 5% bamboo sap (sprayed twice per animal per day), an aqueous solution containing bamboo sap, the bamboo sap content is in mass%, and it is the same concentration of bamboo sap used in step S1 for manufacturing the bamboo sap particle composite.

[0071] 3) is a bamboo sap particle composite (sprayed twice per animal per day), and the spray is obtained by dissolving the precipitate obtained in step S2 of the above application example in 50 mL of distilled water.

[0072] 4) is 5% minoxidil (2 sprays / animal / day), an aqueous solution containing minoxidil, and the minoxidil content is in mass%.

[0073] 5) is apatite (spray twice per animal per day), and the apatite is carbonate apatite.

[0074] Construction of animal models: After adapting all animals to the animal room, the backs of the mice were depilated (depilation area approximately 3 x 2 cm). 2 After shaving, the appropriate drug for each group was sprayed onto the hairless area (with the spray nozzle approximately 6 cm away from the hairless area), and administration began on the day of shaving, once a day for a total of 16 days. On the day of shaving (day 1 of drug administration), and on days 2, 4, 6, 8, 10, 12, 14, 15, 16, and 17 of drug treatment, the hair growth status on the back of the mice was photographed daily.

[0075] Sampling: On day 17, skin tissue was taken from the back of the mice. Each mouse's skin tissue was divided into two parts, and one part was fixed with fixative before being stained with HE pathologically. The expression of IGF-1, VEGF, and FGF-2 was measured by immunohistochemistry.

[0076] The HE staining method described in this invention can utilize methods commonly used in the industry, and optionally, the procedure for the HE staining experiment may be:

[0077] The process involves sectioning, burning, dewaxing and rehydration, staining, and microscopic examination.

[0078] The procedure for the HE staining experiment is as follows:

[0079] 1. Paraffin Sections: Fixed tissue samples are dehydrated for 60 minutes each in 75%, 85%, 95% I, 95% II, 100% I, and 100% II ethanol solutions, then cleared in xylene I and II for 45 minutes each, and finally impregnated in paraffin I, paraffin II, and paraffin III for 50-60 minutes each, before being embedded in paraffin and sectioned.

[0080] 2. Dewaxing and rehydration of paraffin sections: After burning the paraffin sections, place them in xylene I for 10 minutes, xylene II for 10 minutes, anhydrous ethanol I for 3 minutes, anhydrous ethanol II for 3 minutes, 95% alcohol for 3 minutes, 80% alcohol for 3 minutes, and pure water for 2 minutes.

[0081] 3. Hematoxylin staining: Stain in hematoxylin solution for 3-5 minutes, wash with tap water, separate with 1% hydrochloric acid alcohol, wash with tap water, bring out the blue color with a blue coloring agent, and rinse with running water.

[0082] 4. Eosin staining: Place the sections in eosin staining solution and stain for 3-5 minutes, then rinse with running water.

[0083] 5. Dehydration and sealing: The sections are sequentially placed in 80%, 95%, 100% I, and 100% II ethanol solutions for rapid dehydration, and then sealed with a high-quality sealing adhesive.

[0084] 6. Microscopic examination: Take images.

[0085] The staining results showed that the cell nucleus was blue and the cytoplasm was red.

[0086] The immunohistochemical testing method described in this invention can utilize methods commonly used in the industry, and may optionally involve paraffin section immunohistochemistry, the procedure of which is as follows:

[0087] The process involves paraffin embedding of the section, cauterization, dewaxing and rehydration, antigen recovery, removal of endogenous peroxidase, blocking, primary antibody incubation, secondary antibody incubation, DBA staining, restaining, dehydration and clearing, sealing, and microscopic examination.

[0088] 1. Paraffin Sections: Fixed tissue samples are dehydrated for 60 minutes each in 75%, 85%, 95% I, 95% II, 100% I, and 100% II ethanol solutions, then cleared in xylene I and II for 45 minutes each, and finally impregnated in paraffin I, paraffin II, and paraffin III for 50-60 minutes each, before being embedded in paraffin and sectioned.

[0089] 2. Dewaxing and rehydration of paraffin sections: After burning the paraffin sections, place them in xylene I for 10 minutes, xylene II for 10 minutes, anhydrous ethanol I for 3 minutes, anhydrous ethanol II for 3 minutes, 95% alcohol for 3 minutes, 80% alcohol for 3 minutes, and pure water for 2 minutes.

[0090] 3. Antigen recovery of tissue sections: High-pressure recovery (Place the tissue sections in the recovery kit, add antigen recovery solution (citrate buffer), heat in a pressure cooker until the gas is automatically released, remove from the heat source after 2 minutes and allow to cool, discard the antigen recovery solution, and rinse the tissue sections with PBS).

[0091] 4. Removal of endogenous peroxidase: Transfer the sections to a humidified container, add freshly prepared 3% hydrogen peroxide, and remove the endogenous peroxidase inhibitory solution.

[0092] Incubate at room temperature for 10 minutes, then rinse thoroughly with PBS.

[0093] 5. Non-specific blocking: Wash the slide by immersing it in PBS three times for 5 minutes each time. Absorb the PBS around the tissue with absorbent paper, drop 5% BSA onto the slide, and block at 37°C for 30 minutes.

[0094] 6. Application of primary antibody: Absorb the blocking solution around the tissue with absorbent paper, do not wash, and apply a sufficient amount of diluted antibody to each slide.

[0095] Primary antibodies: VEGF (1 / 100) and IGF-1 (1 / 100) are added dropwise, placed in a humidified container, and incubated overnight at 4°C.

[0096] 7. Application of secondary antibody: Remove from the humidified container incubated overnight at 4°C, let stand at room temperature for 45 minutes, wash the slide three times by immersion in PBS for 5 minutes each time, add chili root enzyme-labeled goat anti-rabbit IgG (H+L) (1:100), incubate at 37°C for 30 minutes, and rinse thoroughly with PBS.

[0097] 8. Staining and restaining: Allow to develop color with DAB for 3-5 minutes, assess the degree of staining under a microscope, rinse with PBS or tap water for 1 minute, restain with hematoxylin for 3 minutes, separate with hydrochloric acid alcohol, treat with a bluing agent, and rinse with tap water.

[0098] 9. Dehydration and Clearing: The sections are sequentially placed in 80%, 95%, 100% I, and 100% II ethanol solutions for rapid dehydration, cleared with xylene I and II, sealed with a neutral resin adhesive, and examined under a microscope.

[0099] According to the staining results, hematoxylin-stained cell nuclei are blue, while positive expression by DAB is brownish-yellow.

[0100] Experimental results:

[0101] 1. As shown in Figure 3, 5% bamboo sap and bamboo sap particle complex promoted hair growth on the backs of mice. Hair growth was observed in mice treated with 5% bamboo sap and bamboo sap particle complex on day 14 or day 16 of drug spray treatment, respectively.

[0102] 2. As shown in Figure 4, the changes in mouse hair area were significantly more pronounced in mice treated with 5% bamboo sap and bamboo sap particle complex compared to mice treated with other drugs.

[0103] 3. As shown in Figure 5, the hair growth at AUC-Day12 and AUC-Day16 in the bamboo sap particle complex treatment group showed a significantly greater hair growth effect compared to the control group, the 5% minoxidil group, and the apatite group, in terms of AUC-Day12 for mouse hair growth. AUC-Day16 for the 5% bamboo sap and bamboo sap particle complex treatment groups was clearly higher than that of the 5% minoxidil group or the other groups. (Statistical values ​​are shown as mean ± standard deviation. Student's T-test was used for statistical evaluation, and a P<0.05 result was considered statistically significant.)

[0104] 4. IGF-1 expression, as examined by immunohistochemistry, is shown in Figure 6, and VEGF expression is shown in Figure 7. Compared to the control group and the apatite group, the 5% bamboo sap, bamboo sap particle complex, and 5% minoxidil-treated group all showed increased IGF-1 and VEGF expression. As shown in Figure 8, FGF-2 expression was also shown in the 5% bamboo sap and bamboo sap particle complex compared to the control group, the 5% minoxidil-treated group, and the apatite group.

[0105] Bamboo sap particle composites were manufactured according to the above method, with relative contents of bamboo sap and carbonate apatite particles of 1%, 99%, 16%, 84%, 8%, and 92%, respectively. The above hair growth verification experiments were conducted, and the results were as shown above. The differences are that the hair growth effect of the bamboo sap particle composites with relative contents of 1%, 8%, and 16% increased sequentially, and that the bamboo sap particle composites were superior to single-component bamboo sap compared to the hair growth effect of spraying single bamboo sap with mass percentages of only 1%, 8%, and 16%. [Table 1]

[0106] The "+" symbol indicates the degree of hair growth; the better the hair growth, the more "+" symbols there are.

[0107] Experimental Example 2: Quantitative analysis of bamboo sap contained in bamboo sap particle composites

[0108] As shown in Figure 9, the experimental procedure involved separating the bamboo sap particle composite, which was prepared using 25 mL of 5% bamboo sap (Solution A) by mass, and dissolving the bamboo sap particle composite by adding EDTA, ultimately resulting in a total of 2.8 mL of liquid (Solution B).

[0109] NMR nuclear magnetic resonance (maleic acid internal standard method) measurements were performed on the liquids described above, and the results are shown in Figures 10a and 10b. Figure 10a shows the nuclear magnetic resonance hydrogen spectrum of solution A, and Figure 10b shows the nuclear magnetic resonance hydrogen spectrum of solution B.

[0110] By comparing the nuclear magnetic resonance hydrogen spectra of solution A and solution B, it was found that the ratio of the bamboo sap concentration in solution B to the bamboo sap concentration in solution A is 0.381.

[0111] Solution B contains 5% × 0.381 × 2.8 = 0.0533 g of bamboo sap, which corresponds to 0.2% bamboo sap by mass.

[0112] Experimental Example 3: Research experiment on the effect of 0.2% bamboo sap on hair growth in mice.

[0113] Experimental mouse: C57BL / 6 male, 7 weeks old

[0114] Experimental environment: Temperature 20-26°C, humidity 40-70%

[0115] Experimental grouping: The experiment was divided into two groups, with three animals in each group.

[0116] 1) is the control group, similar to Experimental Example 1, 2) is 0.2% bamboo sap (2 sprays / animal / day), mass %

[0117] Construction of animal models: After adapting all animals to the animal room, the backs of the mice were depilated (depilation area approximately 3 x 2 cm). 2 After shaving, the appropriate drug for each group was sprayed onto the hairless area (with the spray nozzle approximately 6 cm away from the hairless area), and administration began on the day of shaving, once a day for a total of 14 days. On the day of shaving (day 1 of drug administration), and on days 2, 4, 6, 8, 10, 12, and 14 of drug treatment, the hair growth status on the back of the mice was photographed daily.

[0118] Experimental results:

[0119] 1. As shown in Figure 11, the hair growth on the back of the mice did not show any significant hair growth in the mice treated with 0.2% bamboo sap compared to the control group.

[0120] 2. As shown in Figure 12, the mice treated with 0.2% bamboo extract did not show significant hair growth compared to the control group in terms of changes in mouse hair area.

[0121] 3. Regarding hair growth at AUC-Day14, as shown in Figure 13, mice treated with 0.2% bamboo extract did not show a significant hair growth effect at AUC-Day14 compared to the control group. (Statistical values ​​are shown as mean ± standard deviation. Student's T-test was used for statistical evaluation, and a P<0.05 result was considered significant).

[0122] By comparing the results with Experimental Examples 1, 2, and 3, we arrive at the following experimental conclusion: The bamboo sap particle complex of this invention exhibits a better hair growth effect compared to minoxidil. The hair growth effect of the bamboo sap particle complex of this invention is close to that of 5% bamboo sap, but since the bamboo sap particle complex contains only 0.2% bamboo sap, it can be seen that the bamboo sap particle complex can improve the skin penetration of trace amounts of bamboo sap, effectively promote the secretion of IGF-1, and enhance the hair growth effect of bamboo sap.

[0123] Furthermore, since this invention is manufactured using natural bamboo sap, it is safer than conventional techniques that treat hair loss by injecting minoxidil or the direct hair growth factor IGF-1.

[0124] All embodiments described for carrying out the present invention are preferred embodiments of the present invention and do not limit the scope of the present invention. Furthermore, since the above embodiments can be used in combination with each other, all equivalent modifications made in accordance with the structure, shape, and principle of the present invention are included within the scope of the present invention.

Claims

1. A bamboo sap particle composite comprising bamboo sap and an inorganic ion mixture, characterized in that the bamboo sap and the inorganic ion mixture form a particle composite.

2. The inorganic ion mixture is Ca 2+ CO 3 2- , PO 4 3- , Cl - Na + The bamboo sap particle composite according to claim 1, characterized by containing the following:

3. The bamboo sap particle composite according to claim 2, characterized in that the inorganic ion mixture contains carbonate apatite particles.

4. The bamboo sap particle composite according to claim 1, characterized in that the relative content of bamboo sap and inorganic ion mixture is 1 to 16% and 84 to 99% by mass.

5. The bamboo sap particle composite according to claim 4, characterized in that the relative content of bamboo sap and inorganic ion mixture is 3-4% and 96-97% by mass.

6. The bamboo sap particle composite according to claim 5, characterized in that the relative content of the bamboo sap and inorganic ion mixture is 3.25% and 96.75% by mass.

7. The method for producing the bamboo sap includes crushing the bamboo, optionally drying the bamboo, dry distilling the bamboo under reduced pressure, and collecting the distillate. Optionally, the reduced pressure conditions for the carbonization may be 100 mmHg or less, but may also be 10 to 60 mmHg, 15 to 45 mmHg, or 20 mmHg. The carbonization temperature is optionally 180 to 200°C, and optionally the carbonization temperature is 200°C. Optionally, the method further comprises the steps of distilling the distillate and / or further filtering the distillate under reduced pressure. Optionally, the method further comprises diluting the distillate with ethanol. The bamboo sap particle composite according to any one of claims 1 to 6.

8. The bamboo sap production method is characterized by crushing bamboo, drying it as necessary, then dry-distilling the bamboo under reduced pressure conditions and collecting the distillate, wherein the reduced pressure conditions are 100 mmHg or less, preferably 10 to 60 mmHg, more preferably 15 to 45 mmHg, and during reduced-pressure dry-distillation, the distillation temperature of the dry-distillation product depends on the degree of reduced pressure, being lower when the degree of reduced pressure is high and higher when the degree of reduced pressure is low, for example, when dry-distillation is performed under reduced pressure at 20 mmHg, the distillation temperature of the dry-distillation product is 180 to 200°C, as described in any one of claims 1 to 6.

9. A method for producing a bamboo sap particle composite according to any one of claims 1 to 8, S1, a step of preparing a preliminary solution containing bamboo sap and an inorganic ion-containing mixture with distilled water, The process includes the steps of stirring the pre-prepared liquid prepared in S2 and S1, stirring it uniformly, and then centrifuging it so that the resulting precipitate becomes the bamboo sap particle composite, A method for producing a bamboo sap particle composite characterized by the above.

10. In S1, the bamboo sap content is 1.5 to 25% by mass, and the inorganic ion-containing mixture is 100 to 2500 mM. In S2, the stirring temperature is 4 to 37°C, the stirring time is 1 to 30 min, the stirring time decreases as the stirring temperature increases, and the mixture is centrifuged at 3000 to 5000 rpm for 2 to 5 min. The method according to feature 9.

11. In S1, in terms of mass%, the bamboo liquid content is 5%, and the inorganic ion-containing mixture contains 440 mM of NaHCO 3 , 9 mM of NaH 2 PO 4 , 58 mM of CaCl 2 and In S2, the stirring temperature is 20°C, the stirring time is 3 min, and the mixture is centrifuged at 4000 rpm for 3 min. The method according to the present invention, characterized by the present invention.

12. Use of the bamboo sap particle composite according to any one of claims 1 to 8 for manufacturing a hair growth product.

Images