A biosensor strip for sample measurement, its electrode structure, and a sample measurement method utilizing the same.

Abstract

To provide a biosensor strip for a specimen measurement, an electrode structure thereof, and a method for specimen measurement using the electrode structure.SOLUTION: The electrode structure of the biosensor strip according to one embodiment includes: a strip recognition electrode 131 used for recognizing that the biosensor strip has been inserted into a specimen measurement device; a specimen recognition electrode 132 used for recognizing a specimen; and a working electrode 134 and a working reference electrode 135 used for measuring the specimen.SELECTED DRAWING: Figure 4

Description

Technical Field

[0001] The following embodiments relate to a biosensor strip for specimen measurement, its electrode structure, and a specimen measurement method using the same.

Background Art

[0002] Quantitatively and qualitatively analyzing an analyte present in a biological sample (bio sample) can be said to be an important matter both chemically and clinically. For example, measuring blood glucose levels for diabetic patients or measuring cholesterol, which is a risk factor for adult diseases, are typical examples of this.

[0003] Although it is well-known in the art, in an electrochemical biosensor that utilizes the activity of an enzyme, it is extremely important to measure the enzyme activity of a specific substance in a biological sample (hereinafter referred to as "specimen"), such as saliva or blood, for example, glucose, uric acid, protein, DNA, sucrose in clinical chemistry tests, or GOT (Glutamate - Oxaloacetate Transaminase) or GPT (Glutamate - Pyruvate Transaminase) in liver function tests, with rapidity and reproducibility. Here, a biosensor is composed of a recognition site that identifies the measurement target and a change site that converts it into an electrical signal.

[0004] A biological substance is used for the recognition site. When the biological substance recognizes the measurement target, a chemical change or a physical change occurs. The site that converts such a change into an electrical signal is the change site, and the general term for the recognition site and the change site is called a biosensor (Bio sensor) electrode (electrode).

[0005] Currently, the most common measurement method for commercially available strip-type biosensors involves using capillary action, a force stronger than gravity made possible by plasma or chemical surfactant treatment during the manufacturing process, to bring the sample into contact with and flow into the biosensor's sample insertion channel. After accumulating the sample in the sample insertion channel, a sample measurement signal is applied to the working electrode and working reference electrode to measure the response signal to the sample.

[0006] Conventional biosensors, in order to measure a sample, recognize whether the sample has come into contact with the biosensor strip by applying a sample recognition signal to the working electrode and the working reference electrode, thereby recognizing the point of contact and inflow of the sample.

[0007] However, in such cases, the working electrode and working reference electrode used in the sample measurement process are also used in the process of recognizing contact with the sample, so an electric double layer (EDL) may be formed on the surface of the working electrode and working reference electrode before the sample is measured. An electric double layer appears when an electric field is applied at the boundary between different substances (electrode, sample, or solution), and the electric double layer capacitance (DLC), which is the accumulated capacitance of the electric double layer, can be included in the response current signal to the sample that is measured when a sample measurement signal is applied, even if it is only a small amount. Therefore, conventional techniques that form an electric double layer on the working electrode and working reference electrode before sample measurement have the problem of reducing the accuracy of sample measurement.

[0008] This has created a need for technologies that can solve the problems inherent in conventional biosensors. [Overview of the Initiative] [Problems that the invention aims to solve]

[0009] One embodiment proposes an electrode structure for a biosensor strip that includes a strip recognition electrode and a sample recognition electrode, which are individually provided to operate electrically independently of the working electrode and the working reference electrode, in order to improve the accuracy of sample measurement by preventing the formation of an electrical double layer on the surfaces of the working electrode and the working reference electrode before sample measurement.

[0010] Furthermore, in order to miniaturize the biosensor strip, one embodiment proposes an electrode structure for a biosensor strip in which the strip recognition electrode and the sample recognition electrode share a common recognition reference electrode.

[0011] Furthermore, one embodiment relates to the interfacial material properties. Dispensing To improve poor diffusion, we propose an electrode structure for a biosensor strip that includes a dummy electrode to allow the sample to come into contact with the sample insertion channel over a wider area than a pre-set size. However, the technical problems that the present invention aims to solve are not limited to those described above, and may be broadly expanded within the scope of the technical idea and domain of the present invention without departing from it. [Means for solving the problem]

[0012] According to one embodiment, the electrode structure of a biosensor strip for sample measurement may include a strip recognition electrode used to recognize that the biosensor strip has been inserted into a sample measuring instrument, a sample recognition electrode used to recognize a sample, and a working electrode and a working reference electrode used to measure the sample.

[0013] In one aspect, the strip recognition electrode and the sample recognition electrode may be provided separately so as to operate electrically independently with the working electrode and the working reference electrode.

[0014] In other aspects, the working protrusions formed on the working electrode in the region corresponding to the sample insertion channel of the biosensor strip and the at least two or more working reference protrusions formed on the working reference electrode in the region corresponding to the sample insertion channel may be arranged alternately, protruding in one direction perpendicular to the direction in which the working electrode and the working reference electrode are extended.

[0015] Another aspect may be that the recognition reference electrodes for the strip recognition electrode and the sample recognition electrode, respectively, are provided in common with the strip recognition electrode and the sample recognition electrode.

[0016] In another aspect, the sample recognition projection formed in the region of the biosensor strip corresponding to the sample insertion channel of the sample recognition electrode and the recognition reference projection formed in the region of the biosensor strip corresponding to the sample insertion channel of the recognition reference electrode may be characterized in that they are arranged facing each other and protruding in one direction perpendicular to the direction in which the sample recognition electrode and the recognition reference electrode are extended.

[0017] In another aspect, the electrode structure of the biosensor strip may further include a dummy electrode provided on one side of the sample insertion channel of the biosensor strip.

[0018] In another aspect, the strip recognition electrode, the sample recognition electrode, the working electrode, and the working reference electrode may be characterized by being formed to extend in one direction while being parallel and spaced apart from each other.

[0019] According to one embodiment, a biosensor strip for measuring a sample may include a lower plate having an electrode structure that includes a strip recognition electrode used to recognize that the biosensor strip has been inserted into a sample measuring instrument, a sample recognition electrode used to recognize a sample, a working electrode and a working reference electrode used to measure the sample, a middle plate including a sample insertion channel into which an enzyme compound that reacts with the sample is inserted when positioned above the lower plate, and an upper plate including an insertion port for inserting the sample into the sample insertion channel when positioned above the middle plate.

[0020] In one aspect, the strip recognition electrode and the sample recognition electrode on the lower plate may be provided separately so as to operate electrically independently with the working electrode and the working reference electrode.

[0021] In other respects, the recognition reference electrodes for the strip recognition electrode and the sample recognition electrode on the lower plate may be characterized in that they are provided in common for both the strip recognition electrode and the sample recognition electrode.

[0022] In another aspect, the lower plate may further include a dummy electrode provided on one side of the sample insertion channel of the biosensor strip.

[0023] According to one embodiment, the sample measurement system may include a biosensor strip having an electrode structure that includes a strip recognition electrode used to recognize that the biosensor strip has been inserted into the sample measurement device, a sample recognition electrode used to recognize a sample, a working electrode and a working reference electrode used to measure the sample, and the sample measurement device which recognizes whether the biosensor strip has been inserted, recognizes whether a sample has come into contact with the biosensor strip if the biosensor strip has been inserted into the sample measurement device, and if the sample has come into contact with the biosensor strip, applies a sample measurement signal to the working electrode and the working reference electrode to measure a response signal to the sample.

[0024] According to one embodiment, a specimen measurement method executed by a specimen measurement system including a biosensor strip and a specimen measurement device includes: recognizing whether the specimen measurement device is inserted into the biosensor strip by using a strip recognition electrode included in the biosensor strip; when the biosensor strip is inserted into the specimen measurement device, recognizing whether a specimen contacts the biosensor strip by using a specimen recognition electrode included in the biosensor strip; and when the specimen contacts the biosensor strip, measuring a response signal for the specimen by applying a specimen measurement signal to a working electrode and a working reference electrode included in the biosensor strip.

[0025] According to one aspect, the step of measuring the response signal for the specimen is independently executed from the step of recognizing whether the biosensor strip is inserted into the specimen measurement device and the step of recognizing whether the specimen contacts the biosensor strip by being separately provided such that the strip recognition electrode and the specimen recognition electrode operate electrically separately from the working electrode and the working reference electrode.

[0026] According to another aspect, the specimen measurement method may further include the step of widely contacting the specimen with a size greater than or equal to a preset size in a specimen insertion channel of the biosensor strip by using a dummy electrode provided on one side of the specimen insertion channel of the biosensor strip.

Advantages of the Invention

[0027] One embodiment can achieve the technical effect of preventing the formation of an electric double layer on the surfaces of the working electrode and the working reference electrode before specimen measurement and improving the accuracy of specimen measurement by proposing an electrode structure of a biosensor strip including a strip recognition electrode and a specimen recognition electrode that are separately provided to operate electrically separately from the working electrode and the working reference electrode.

[0028] Furthermore, one embodiment proposes an electrode structure for a biosensor strip in which the strip recognition electrode and the sample recognition electrode share a common recognition reference electrode, thereby achieving the technical effect of miniaturizing the biosensor strip.

[0029] Furthermore, one embodiment proposes an electrode structure for a biosensor strip that includes a dummy electrode to bring the sample into contact with the sample insertion channel over a wider area than a predetermined size, thereby achieving the technical effect of improving poor dispensing and diffusing properties with respect to interfacial material properties.

[0030] However, the effects of the present invention are not limited to those described above and may be extended in various ways without departing from the technical idea and scope of the present invention. [Brief explanation of the drawing]

[0031] [Figure 1] This figure shows a sample measurement system in one embodiment. [Figure 2] Figure 1 shows the sample collection equipment. [Figure 3] This figure shows the biosensor strip shown in Figure 1. [Figure 4] This figure shows the electrode structure of the lower plate of the biosensor strip shown in Figure 3. [Figure 5] This is a flowchart illustrating a sample measurement method in one embodiment. [Figure 6] Figure 5 shows an example illustrating the sample measurement method. [Modes for carrying out the invention]

[0032] Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. However, the present invention should not be limited or restricted by these embodiments. The same reference numerals in the drawings indicate the same components.

[0033] Furthermore, the terminology used herein is intended to adequately describe preferred embodiments of the invention, but this may vary depending on the intent of the audience or operator, or the conventions of the art to which the invention belongs. Therefore, the definitions of such terms should be based on the overall content of this specification. For example, the singular form as used herein includes the plural form unless otherwise specified in the text. Also, the terms “comprises” and / or “comprising” as used herein do not mean that the components, stages, actions, and / or elements referred to exclude the existence or addition of one or more other components, stages, actions, and / or elements. Furthermore, terms such as “first,” “second,” etc., are used herein to describe various areas, directions, shapes, etc., but such areas, directions, shapes should not be limited by such terms. These terms are merely used to distinguish one given area, direction, or shape from other areas, directions, or shapes. Therefore, a part referred to as the first part in one embodiment may be referred to as the second part in another embodiment.

[0034] Furthermore, it should be understood that the diverse embodiments of the present invention, while distinct from one another, do not necessarily have to be mutually exclusive. For example, certain shapes, structures, and characteristics described herein may be realized in other embodiments in relation to one embodiment, without departing from the technical spirit and scope of the present invention. Also, it should be understood that the position, arrangement, or configuration of individual components within the scope of each presented embodiment can be modified without departing from the technical spirit and scope of the present invention.

[0035] The biosensor strip according to the embodiment, its electrode structure, a sample measurement system including the same, and a sample measurement method using the same will be described below with reference to the drawings.

[0036] Figure 1 shows a sample measurement system according to one embodiment, Figure 2 shows the sample collection device shown in Figure 1, Figure 3 shows the biosensor strip shown in Figure 1, and Figure 4 shows the electrode structure of the lower plate of the biosensor strip shown in Figure 3.

[0037] A sample measurement system 100 according to one embodiment may include a sample measurement device 110, a biosensor strip 120, and a sample collection device 160.

[0038] As shown in Figure 2, the sample collection device 160 includes a sample collection unit 161 containing a sample collection swab 161-1 for collecting samples, a filter 162, and a compression tube 163.

[0039] In this case, if the sample is saliva, the sample collection unit 161 may collect the saliva through the sample collection swab 161-1. The saliva collected by the sample collection unit 161 may have interfering substances removed from it via a filter 162 inserted into the compression tube 163, and then be provided to the outside, i.e., to the biosensor strip 120, through the compression tube 163.

[0040] In other words, after collecting saliva, the sample collection device 160 compresses the sample collection unit 161 in the direction of the compression tube 163 within the compression tube 163, and can remove interfering substances contained in the saliva via the filter 162.

[0041] The filter 162 may consist of at least one layer. In particular, the thickness or number of layers of the filter 162 may be adjusted considering the degree to which interfering substances to be removed from the sample are to be removed, and the degree of filtering may be adjusted considering the size of the interfering substances to be removed from the sample. For example, if the goal is to remove large molecules and foreign matter present in saliva as interfering substances, the filter 162 may be formed to have a filtering degree that is sufficient to remove large molecules and foreign matter.

[0042] When a sample collected by the sample collection device 160 comes into contact with and is inserted through the sample collection device 160, the biosensor strip 120 may provide the sample measuring device 110 with a response signal in response to the strip recognition signal received from the sample measuring device 110, a response signal in response to the sample recognition signal received from the sample measuring device 110, and a response signal in response to the sample measurement signal received from the sample measuring device 110, while inserted into the sample measuring device 110. This allows the sample measuring device 110 to recognize the insertion of the biosensor strip 120 and contact with the sample based on the response signals received from the biosensor strip 120, and to measure the sample.

[0043] Such a biosensor strip 120 may consist of a lower plate 130, a middle plate 140, and an upper plate 150, as shown in Figure 3. More specifically, as shown in Figure 4, the biosensor strip 120 may include a lower plate 130 on which an electrode structure is formed, a middle plate 140 located above the lower plate 130 and including a sample insertion channel 141 into which an enzyme compound that reacts with the sample is inserted, and an upper plate 150 located above the middle plate 140 and including an insertion port 151 for inserting the sample into the sample insertion channel 141 and an outlet port 152 for discharging air.

[0044] Here, the sample insertion channel 141 is obvious to those skilled in the art, so a detailed explanation thereof will be omitted.

[0045] The enzyme compound 142 may include an enzyme for selectively reacting with glucose contained in the sample, a polymer for attaching the enzyme to the electrode placed on the lower plate 130, and a catalyst for promoting the reaction between the enzyme and glucose.

[0046] In this case, the catalyst may contain 1 to 10% of at least one of ferrocene, ferrocene derivatives, quinone, quinone derivatives, hexaamineruthenium(III) chloride, Prussian blue, or ferricyanide (if Prussian blue is used, the catalyst may contain 0.001 to 5% Prussian blue); the enzyme may contain 0.001 to 5% by weight of at least one of glucose oxidase and glucose dehydrogenase (GDH); and the polymer may contain 1 to 30 units of at least one of Chitosan, PVP, Nafion, polyethylene glycol, polyvinyl pyrrolidone, polyvinyl alcohol, agarose, or trehalose.

[0047] The lower plate 130 may have an electrode structure formed thereon, which includes a strip recognition electrode 131, a sample recognition electrode 132, a recognition reference electrode 133, an action electrode 134, an action reference electrode 135, a dummy electrode 136, and spare electrodes 137 and 138.

[0048] The strip recognition electrode 131 may be used to recognize whether the biosensor strip 120 has been inserted into the sample measuring device 110. For example, a strip recognition signal may be applied from the sample measuring device 110 through the strip recognition electrode 131 and the recognition reference electrode 133, and a response signal in response to this may be provided from the biosensor strip 120 to the sample measuring device 110 through the strip recognition electrode 131 and the recognition reference electrode 133. Thus, the sample measuring device 110 can use the strip recognition electrode 131 to recognize whether the biosensor strip 120 has been inserted into the sample measuring device 110. Whether or not the biosensor strip 120 has been inserted into the sample measuring device 110 may be determined by the intensity of the response signal in response to the strip recognition signal, or by the generation and provision status of the response signal.

[0049] The sample recognition electrode 132 may be used to recognize a sample. More specifically, the sample recognition electrode 132 may be used to recognize whether a sample has come into contact with the biosensor strip 120. For example, a sample recognition signal may be applied from the sample measuring instrument 110 through the sample recognition electrode 132 and the recognition reference electrode 133, and a response signal in response to this signal may be provided from the biosensor strip 120 to the sample measuring instrument 110 through the sample recognition electrode 132 and the recognition reference electrode 133. Thus, the sample measuring instrument 110 can use the sample recognition electrode 132 to recognize whether a sample has come into contact with the biosensor strip 120. Whether a sample has come into contact with the biosensor strip 120 may be determined by the intensity of the response signal in response to the sample recognition signal, or by the occurrence and provision of the response signal.

[0050] The sample recognition projection 132-1 formed on the sample recognition electrode 132 in the region corresponding to the sample insertion channel 141 of the biosensor strip 120, and the recognition reference projection 133-1 formed on the recognition reference electrode 133 in the region corresponding to the sample insertion channel 141 of the biosensor strip 120, may be arranged facing each other at a distance from each other, with the projections protruding in one direction perpendicular to the direction in which the sample recognition electrode 132 and the recognition reference electrode 133 are extended.

[0051] In this case, the recognition reference electrode 133 for the strip recognition electrode 131 and the sample recognition electrode 132 may be provided in common for both the strip recognition electrode 131 and the sample recognition electrode 132. That is, the strip recognition electrode 131 and the sample recognition electrode 132 may share one recognition reference electrode 133. This makes it possible to miniaturize the biosensor strip 120, unlike when the strip recognition electrode 131 and the sample recognition electrode 132 each have their own recognition reference electrode.

[0052] The working electrode 134 and the working reference electrode 135 may be used to measure the sample. Specifically, a sample measurement signal is applied from the sample measuring device 110 through the working electrode 134 and the working reference electrode 135, and a response signal for the sample in response to this signal is provided from the biosensor strip 120 to the sample measuring device 110 through the working electrode 134 and the working reference electrode 135. Therefore, the sample measuring device 110 can measure the sample by measuring the response signal for the sample using the working electrode 134 and the working reference electrode 135. The sample measurement result may be determined by the intensity of the response signal in response to the sample measurement signal, or by the generation and provision of the response signal. For example, the sample measurement result may be shown as a multi-level measurement value depending on the intensity of the response signal in response to the sample measurement signal.

[0053] The working electrode 134-1 formed in the region of the biosensor strip 120 corresponding to the sample insertion channel 141, and the two or more working reference protrusions 135-1, 135-2 formed in the region of the working reference electrode 135 corresponding to the sample insertion channel 141, may be arranged alternately, protruding in one direction perpendicular to the direction in which the working electrode 134 and the working reference electrode 135 are extended.

[0054] Thus, the device is configured such that one working projection 134-1 is positioned between two or more working reference projections 135-1 and 135-2, and they are arranged alternately. By forming the device so that the area of ​​one working projection 134-1 is smaller than the area of ​​each of the two or more working reference projections 135-1 and 135-2, the device can accurately measure the sample measurement signal and the response signal that responds to the sample measurement signal when the sample comes into contact with the device. This improves the accuracy of sample measurement.

[0055] The strip recognition electrode 131, sample recognition electrode 132, recognition reference electrode 133, working electrode 134, and working reference electrode 135 described above may be formed by extending in one direction while being parallel and spaced apart from each other.

[0056] In the electrode structure described above, the strip recognition electrode 131 and the sample recognition electrode 132 may be individually provided to operate electrically independently with the working electrode 134 and the working reference electrode 135. Therefore, the accuracy of sample measurement can be improved by preventing the formation of an electrical double layer on the surfaces of the working electrode 134 and the working reference electrode 135 before sample measurement.

[0057] The dummy electrode 136 is intended to improve poor dispensing and diffusion properties with respect to the interfacial material properties by bringing the sample into contact with the sample insertion channel 141 over a wider area than a predetermined size, and may be provided on one side of the region of the lower plate 130 corresponding to the sample insertion channel 141.

[0058] The spare electrodes 137 and 138 are electrodes provided as spares in the electrode structure of the biosensor strip 120 described above, and may be used as one of the strip recognition electrode 131, sample recognition electrode 132, recognition reference electrode 133, working electrode 134, and working reference electrode 135 as needed.

[0059] Although not shown in the drawings, the lower plate 130 on which the electrode structure described above is formed may be formed by stacking multiple metal layers. For example, the lower plate 130 may be realized as a four-layer structure by stacking an insulating substrate layer (not shown), a first metal layer (not shown) made of copper formed on the insulating substrate layer, a second metal layer (not shown) made of nickel formed on the first metal layer, and a third metal layer (not shown) made of gold formed on the second metal layer. In this case, the electrode structure described above may be formed on each of the first, second, third, and fourth metal layers.

[0060] The sample measuring device 110, upon insertion of the biosensor strip 120 into which the sample has come into contact and into which the sample has been introduced, sequentially applies a strip recognition signal, a sample recognition signal, and a sample measurement signal to the biosensor strip 120. By receiving response signals for each of the strip recognition signal, sample recognition signal, and sample measurement signal, the device recognizes that the biosensor strip 120 has been inserted and that the sample has come into contact with the biosensor strip 120, and then measures the sample.

[0061] The results of measuring the sample may be provided as multiple measurement values ​​depending on the intensity of the response signal in response to the sample measurement signal, and different graphic effects may be applied depending on the measurement value, and these may be displayed through the display means of the sample measuring device 110.

[0062] Figure 5 is a flowchart showing a sample measurement method according to one embodiment, and Figure 6 is a diagram showing an example to illustrate the sample measurement method shown in Figure 5. The sample measurement method described below is assumed to be mainly performed by the sample measurement system 100 described with reference to Figures 1 to 4.

[0063] Before steps 510-530, the sample measurement system 100 (more precisely, the sample collection device 160) may collect the subject's saliva using the sample collection unit 161 of the sample collection device 160, as shown in Figures 6(a) and (b), and remove interfering substances from the saliva sample by inserting it into a compression tube 163 equipped with a filter 162. Next, the biosensor strip 120 may be inserted into the sample measurement device 110 as shown in Figure 6(c), and the sample may be inserted and flowed from the sample collection device 160 into the insertion port 151 of the biosensor strip 120, as shown in Figure 6(d), thereby bringing the sample into contact with the sample insertion channel 141 of the biosensor strip 120.

[0064] At this time, the sample inserted into and flowing into the biosensor strip 120 can be brought into contact with the sample insertion channel 141 of the biosensor strip 120 over a width greater than a preset size by the dummy electrode 136 provided on one side of the sample insertion channel 141 of the biosensor strip 120.

[0065] In step 510, the sample measurement system 100 (more precisely, the sample measurement device 110) may recognize whether the biosensor strip 120 has been inserted into the sample measurement device 110 by utilizing the strip recognition electrode 131 contained in the biosensor strip 120. More specifically, the sample measurement device 110 may recognize and determine whether the biosensor strip 120 has been inserted into the sample measurement device 110 by applying a strip recognition signal through the strip recognition electrode 131 and the recognition reference electrode 133, and receiving a response signal in response from the biosensor strip 120 through the strip recognition electrode 131 and the recognition reference electrode 133.

[0066] In step 520, the sample measurement system 100 (more precisely, the sample measurement device 110) may, when the biosensor strip 120 is inserted into the sample measurement device 110 (when it is recognized in step 510 that the biosensor strip 120 has been inserted into the sample measurement device 110), use the sample recognition electrode 132 contained in the biosensor strip 120 to recognize whether a sample has come into contact with the biosensor strip 120. More specifically, the sample measurement device 110 may recognize and determine whether a sample has come into contact with the biosensor strip 120 by applying a sample recognition signal through the sample recognition electrode 132 and the recognition reference electrode 133, and by providing a response signal in response to this signal from the biosensor strip 120 through the sample recognition electrode 132 and the recognition reference electrode 133.

[0067] In step 530, if the sample comes into contact with the biosensor strip 120 (or more precisely, if the biosensor strip 120 is recognized to have come into contact with the sample in step 520), the sample measurement system 100 (or more precisely, the sample measurement device 110) may apply a sample measurement signal to the working electrode 134 and the working reference electrode 135 contained in the biosensor strip 120, as shown in Figure 6(e), to measure the response signal to the sample. More specifically, the sample measurement device 110 may measure the sample by applying a sample measurement signal through the working electrode 134 and the working reference electrode 135, and measuring the response signal in response to this signal provided from the biosensor strip 120 through the working electrode 134 and the working reference electrode 135.

[0068] In particular, step 530, which measures the response signal to the sample, can be performed independently of step 510, which recognizes whether the biosensor strip 120 has been inserted into the sample measuring instrument 110, and step 520, which recognizes whether the sample has come into contact with the biosensor strip 120, by individually providing the strip recognition electrode 131 and the sample recognition electrode 132 to operate electrically independently with the working electrode 134 and the working reference electrode 135.

[0069] As described above, embodiments have been explained based on limited embodiments and drawings, but those skilled in the art will be able to make various modifications and variations from the above description. For example, the described technique may be performed in a different order than described, and / or the components of the described system, structure, apparatus, circuit, etc. may be combined or assembled in a different manner than described, or opposed or replaced by other components or equivalents, and still achieve suitable results.

[0070] Therefore, even if the embodiment is different, it falls within the scope of the attached claims if it is equivalent to the claims.

Claims

1. A biosensor for detecting glucose in saliva, A working electrode and a working reference electrode for detecting glucose, wherein the working electrode includes at least one working projection, and the working reference electrode includes at least one working reference projection; A sample recognition electrode for recognizing the aforementioned saliva, wherein the sample recognition electrode includes at least one sample recognition projection; At least one dummy electrode; and The substrate on which the working electrode, the working reference electrode, the sample recognition electrode, and the dummy electrode are arranged. Equipped with, At least a portion of each of the action projection, the action reference projection, the sample recognition projection, the dummy electrode, and the substrate constitutes a region corresponding to the sample insertion channel of the biosensor. A reagent layer is placed in the aforementioned sample insertion channel. At least a portion of the dummy electrode is a biosensor for detecting glucose in saliva, positioned at its longitudinal end within a region corresponding to the sample insertion channel.

2. A biosensor for detecting glucose in saliva, A working electrode and a working reference electrode for detecting glucose, wherein the working electrode includes at least one working projection, and the working reference electrode includes at least one working reference projection, A sample recognition electrode for recognizing the aforementioned saliva, wherein the sample recognition electrode includes at least one sample recognition projection, A lower plate containing at least one dummy electrode, An intermediate plate is located above the aforementioned lower plate and includes a sample insertion channel in which a reagent layer is placed, An upper plate is positioned above the middle plate and includes a specimen insertion port and an air outlet, Equipped with, At least a portion of each of the aforementioned action projection, action reference projection, specimen recognition projection, dummy electrode, and lower plate constitutes a region corresponding to the specimen insertion channel. At least a portion of the dummy electrode is a biosensor for detecting glucose in saliva, positioned at its longitudinal end within a region corresponding to the sample insertion channel.

3. In claim 1, A biosensor for detecting glucose in saliva, wherein at least a portion of the action projection, the action reference projection, the sample recognition projection, the dummy electrode, and the substrate each constitutes a region corresponding to the reagent layer.

4. In claim 2, A biosensor for detecting glucose in saliva, wherein at least a portion of the action projection, the action reference projection, the sample recognition projection, the dummy electrode, and the lower plate constitutes a region corresponding to the reagent layer.

5. In claim 3 or 4, The region corresponding to the reagent layer overlaps with the region corresponding to the sample insertion channel, and is a biosensor for detecting glucose in saliva.

6. In claim 3 or 4, The region corresponding to the reagent layer is a biosensor for detecting glucose in saliva, which coincides with the region corresponding to the sample insertion channel.

7. In claim 1 or 2, A biosensor for detecting glucose in saliva, wherein the reagent layer comprises an enzyme for selectively reacting with the glucose, a polymer for attaching the enzyme to a region corresponding to the reagent layer, and a catalyst for promoting the reaction between the enzyme and the glucose.

8. In claim 1 or 2, The reagent layer is a biosensor for detecting glucose in saliva, which contacts a region corresponding to the sample insertion channel with the dummy electrode over a size greater than or equal to a predetermined size.

9. In claim 1 or 2, The dummy electrode is a biosensor for detecting glucose in saliva, configured to improve the dispensing and diffusivity of the reagent layer.

10. In claim 1, At least a portion of the dummy electrode is positioned in a region on the substrate corresponding to the sample insertion port of the biosensor, and is a biosensor for detecting glucose in saliva.

11. In claim 2, At least a portion of the dummy electrode is placed in the region on the lower plate corresponding to the sample insertion port. A biosensor for detecting glucose in saliva.

12. In claim 1 or 2, A biosensor for detecting glucose in saliva, wherein at least the portion of the dummy electrode other than the portion constituting the region corresponding to the sample insertion channel is located outside the region corresponding to the sample insertion channel, extending beyond the region corresponding to the sample insertion channel.

13. In claim 1 or 2, In the region corresponding to the sample insertion channel, the action projection and the action reference projection are arranged alternately. The sample recognition projection is a biosensor for detecting glucose in saliva, positioned adjacent to the action projection or the action reference projection, and spaced parallel to the action projection and the action reference projection.

14. In claim 13, The aforementioned action projections and the aforementioned action reference projections are biosensors for detecting glucose in saliva, with each having a different number and area.

15. In claim 1 or 2, The aforementioned sample recognition electrode is a biosensor for detecting glucose in saliva, which is used to recognize the saliva.

16. In claim 1, Further equipped with strip recognition electrodes, The strip recognition electrode is for recognizing the biosensor, and the biosensor is for detecting glucose in saliva.

17. In claim 2, The aforementioned lower plate further comprises strip recognition electrodes, The strip recognition electrode is for recognizing the biosensor, and the biosensor is for detecting glucose in saliva.

18. In a system for measuring glucose concentration in saliva, Sample collection equipment, A biosensor according to claim 1 or 2, Sample measuring equipment, A system for measuring glucose concentration in saliva, including [specific component / tool].

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